OPTIMIZATION OF INTRANASAL COVID-19 VACCINE FORMULATION WITH Lactococcus lactis pNZ HCR BACTERIA AS VECTOR IN LIQUID AEROSOL PREPARATION

Main Article Content

Valentina Yurina

Abstract

Vaccination is an effective method to suppress COVID-19 transmission, but injection-based vaccination is less effective due to its inability to induce mucosal immunity. This study aimed to determine the effects of vaccine formulations on bacteria viability and antigen expression to find the optimal formulation. Three intranasal preparation formulations (F1, F2, and F3) were created with different ingredient compositions, along with a control. Physicochemical tests were conducted on day 0 and day 14 to assess bacterial viability, and antigen expression was evaluated using the western blot method. Formula 2, containing sodium alginate (0.615%), trehalose (4.125%), polyvinyl alcohol (0.1%), and calcium chloride (5%), exhibited the best viability test results, although no significant differences were observed among the groups. The study concluded that variations in composition concentrations could affect bacterial stability, with Formula 2 showing the best results in terms of bacteria viability and antigen expression up to 14 days after formulation.

Article Details

How to Cite
Yurina, V. (2023). OPTIMIZATION OF INTRANASAL COVID-19 VACCINE FORMULATION WITH Lactococcus lactis pNZ HCR BACTERIA AS VECTOR IN LIQUID AEROSOL PREPARATION. Jurnal Bioteknologi Dan Biosains Indonesia, 10(1), 97–104. Retrieved from https://ejournal.brin.go.id/JBBI/article/view/1742
Section
Articles

References

Annas S, Zamri-Saad M (2021) Intranasal vaccination strategy to control the covid-19 pandemic from a veterinary medicine perspective. Animals 11. doi: 10.3390/ani11071876

Azizpour M, Hosseini SD, Jafari P, Akbary N (2017) Lactococcus lactis: A new strategy for vaccination. Avicenna J Med Biotechnol 9:163–168

Baraniuk C (2021) How long does covid-19 immunity last? BMJ 373:n1605. https://doi.org/10.1136/bmj.n1605

Carvalho RDDO, do Carmo FLR, de Oliveira Junior A, Langella P, Chatel J-M, Bermúdez-Humarán LG, Azevedo V, de Azevedo MS (2017) Use of Wild Type or Recombinant Lactic Acid Bacteria as an Alternative Treatment for Gastrointestinal Inflammatory Diseases: A Focus on Inflammatory Bowel Diseases and Mucositis. Front Microbiol 8. https://doi.org/10.3389/fmicb.2017.00800

Fuad E, Gunawan R, Amien J Al, Elviani U (2019) Perangkat Media Terapi Bagi Anak Penderita Fobia Jarum Suntik (Trypanophobia) Menggunakan Teknologi Augmented Reality. J MEDIA Inform BUDIDARMA 3:48–53. https://doi.org/10.30865/mib.v3i1.1063

Haindl R, Neumayr A, Frey A, Kulozik U (2020) Impact of cultivation strategy, freeze-drying process, and storage conditions on survival, membrane integrity, and inactivation kinetics of Bifidobacterium longum. Folia Microbiol (Praha) 65:1039–1050. doi: 10.1007/s12223-020-00815-3

Her JY, Kim MS, Lee KG (2015) Preparation of probiotic powder by the spray freeze-drying method. J Food Eng 150:70–74. https://doi.org/10.1016/j.jfoodeng.2014.10.029

Ishrath A, Ahmed MM, Pal N, Muppidi S (2021) Covid-19 (Pandemic): A Review Article. J Res Med Dent Sci 9:281–288

Ishrath A, Ahmed MM, Pal N, Muppidi S (2021) Covid-19 (Pandemic): A Review Article. J Res Med Dent Sci 9:281–288

Jeyanathan M, Afkhami S, Smaill F, Miller MS, Lichty BD, Xing Z (2020) Immunological considerations for COVID-19 vaccine strategies. Nat Rev Immunol 20:615–632. doi: 10.1038/s41577-020-00434-6

Li L, Ma Y (2014) Effects of metal ions on growth, B-oxiation system, and thioesterase activity of Lactococcus lactis. J Dairy Sci 97:5975–5982. doi: 10.3168/jds.2014-8047

Mancha-Agresti P, de Catro CP, dos Santos JSC Arauja MA, Pereira VB, LeBlanc JG, Leclercq SY, Azevedo V V (2017) Recombinant invasive Lactococcus lactis carrying a DNA vaccine coding the Ag85A antigen increases INF-y, IL- 6, and TNF-a cytokines after intranasal immunization. Front Microbiol 8:1–12. doi: 10.3389/fmicb.2017.01263

Mendoza GM, Pasteris SE, Otero MC, Fatima Nader-Macías ME (2013) Survival and beneficial properties of lactic acid bacteria from raniculture subjected to freeze-drying and storage. J Appl Microbiol 116:157–166. https://doi.org/10.1111/jam.12359

Moreno-Fierros L, García-Silva I, Rosales-Mendoza S (2020) Development of SARS-CoV-2 vaccines: should we focus on mucosal immunity? Expert Opin Biol Ther 20:831–836. https://doi.org/10.1080/14712598.2020.1767062

Mudgal R, Nehul S, Tomar S (2020) Prospects for mucosal vaccine: shutting the door on SARS-CoV-2. Hum Vaccines Immunother 16:2921–2931. https://doi.org/10.1080/21645515.2020.1805992

Nagpal PS, Kesarwani A, Sahu P, Upadhyay P (2019) Aerosol immunization by alginate coated mycobacterium (BCG/MIP) particles provide enhanced immune response and protective efficacy than aerosol of plain mycobacterium against M.tb. H37Rv infection in mice. BMC Infect Dis 19:1–14. https://doi.org/10.1186/S12879-019-4157-2/FIGURES/6

Rockinger U, Funk M, Winter G (2021) Current Approaches of Preservation of Cells During (freeze-) Drying. J Pharm Sci 110:2873–2893. doi: 10.1016/j.xphs.2021.04.018

Rowe RC, Sheskey PJ, Quinn ME (2009) Handbook of Pharmaceutical Excipients, 6th edn. Pharmaceutical Press, London

Russell MW, Moldoveanu Z, Ogra PL, Mestecky J (2020) Mucosal Immunity in COVID-19: A Neglected but Critical Aspect of SARS-CoV-2 Infection. Front Immunol 11. https://doi.org/10.3389/fimmu.2020.611337

Rzymski P, Camargo CA, Fal A, Flisiak R, Gwenzi W, Kelishadi R, Leemans A, Nieto JJ, Ozen A, Perc M, Poniedzia?ek B, Sedikides C, Sellke F, Skirmuntt EC, Stashchak A, Rezaei N (2021) COVID-19 Vaccine Boosters: The Good, the Bad, and the Ugly. Vaccines 2021, Vol 9, Page 1299 9:1299. https://doi.org/10.3390/VACCINES9111299

Shah SM, Alsaab HO, Rawas-Qalaji MM, Uddin MN (2021) A review on current covid-19 vaccines and evaluation of particulate vaccine delivery systems. Vaccines 9. doi: 10.3390/vaccines9101086

Shi W, Kou Y, Jiang H, Gao F, Kong W, Su W, Xu F, Jiang C (2018) Novel intranasal pertussis vaccine based on bacterium-like particles as a mucosal adjuvant. Immunol Lett 198:26–32. https://doi.org/10.1016/J.IMLET.2018.03.012

Shigemori S, Shimosato T (2017) Applications of Genetically Modified Immunobiotics with High Immunoregulatory Capacity for Treatment of Inflammatory Bowel Diseases. Front Immunol 8:25. https://doi.org/10.3389/fimmu.2017.00022

Soysal G, Durukan E, Akdur R (2021) The evaluation of vaccine hesitancy and refusal for childhood vaccines and the covid-19 vaccine in individuals aged between 18 and 25 years. Turkish J Immunol 9:120–127. doi: 10.4274/tji.galenos.2021.35229

Tan EW, Tan KY, Phang LV, Kumar PV, In LLA (2019) Enhanced gastrointestinal survivability of recombinant Lactococcus lactis using a double coated mucoadhesive film approach. PLoS One 14:1–15. doi: 10.1371/journal.pone.0219912

Wyszynska A, Kobierecka P, Bardowski J, Jagusztyn-Krynicka EK (2015) Lactic acid bacteria—20 years exploring their potential as live vectors for mucosal vaccination. Appl Microbiol Biotechnol 99:2967–2977. https://doi.org/10.1007/s00253-015-6498-0

Yurina V (2020) Coronavirus epitope prediction from highly conserved region of spike protein. Clin Exp Vaccine Res 9:169–173. https://doi.org/10.7774/cevr.2020.9.2.169

Yurina V, Adianingsih OR, Widodo N (2021) Oral and Intranasal Immunization with Recombinant Food-Grade Lactococcus Lactis Expressing High Conserved Region of SARS-CoV-2 Spike Protein Triggers Immunity Responses in Mice. Res Sq 1–13. https://doi.org/10.21203/rs.3.rs-951426/v1