ANTIBAKTERI EKSTRAK KAPANG ENDOFIT DARI AKAR KAYU JAWA (Lannea coromandelica (Houtt.) Merr.)
Main Article Content
Abstract
Isolation of endophytic fungi from the roots of Lannea coromandelica could be one way to know the presence of antibacterial activity that serves as a traditional medicinal remedy. This study aims to determine the presence of endophytic fungi at the root of L. coromandelica and their extract potential as an antibacterial agent. Endophytic fungi isolation was performed by direct technique and their macroscopic as well as microscopic characteristics were observed. Screening tests were conducted using methanol and ethyl acetate solvents. Antibacterial tests were conducted on Escherichia coli, Bacillus subtilis, and Staphylococcus epidermidis. This study resulted in seven isolates of endophytic fungi (RLC 1A, RLC 1B, RLC 1C, RLC 2, RLC 3, RLC 4, RLC 5) originated from the root of L. coromandelica. RLC 5 isolate exhibited the largest diameter of inhibition activity on the screening tests and the largest diameter on the antibacterial tests. The supernatant extract of RLC 5 isolate showed the highest antibacterial activity against the test bacterium S. epidermidis.
Isolasi kapang endofit dari akar Lannea coromandelica dapat menjadi salah satu cara untuk mengetahui adanya aktivitas antibakteri yang berfungsi sebagai obat tradisional. Penelitian ini bertujuan untuk mengetahui keberadaan kapang endofit pada akar L. coromandelica dan potensinya sebagai agen antibakteri. Isolasi kapang endofit dilakukan dengan teknik langsung lalu diamati karakteristik makroskopik dan mikroskopiknya. Uji penapisan dilakukan menggunakan pelarut metanol dan etil asetat. Uji antibakteri dilakukan terhadap Escherichia coli, Bacillus subtilis, dan Staphylococcus epidermidis. Penelitian ini mendapatkan tujuh isolat kapang endofit (RLC 1A, RLC 1B, RLC 1C, RLC 2, RLC 3, RLC 4, RLC 5) yang berasal dari akar L. coromandelica. Isolat RLC 5 memperlihatkan diameter daya hambat terbesar pada uji penapisan dan diameter terbesar pada uji aktivitas antibakteri. Ekstrak supernatan isolat RLC 5 menunjukkan aktivitas antibakteri tertinggi terhadap bakteri uji S. epidermidis.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
a). Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Attribution-NonCommercial-ShareAlike 4.0 International that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b). Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
References
Alam B, Hossain S, Habib R, Rea J, Islam A (2012) Antioxidant and analgesic activities of Lannea coromandelica Linn. Bark extract. Int J Pharmacol 8: 224–233. doi:10.3923/ijp.2012.224.233
Amelia P, Ivada PAK, Fitriana N, Komala I, Bahri S, Hanafi M (2021) Antioxidant and antimicrobial activity of secondary metabolite produced by endophytic fungi isolated from Lannea coromandelica (Houtt.) Merr. Int J Pharm Sci Res 12: 1588–1592. doi:10.13040/IJPSR.0975-8232.12(3).1588-92
Bahri S, Amelia P, Hardini A, Ramadhan F, Muhammad AA (2021) Aktivitas antibakteri kapang endofit dari kulit batang tanaman Kayu Jawa (Lannea coromandelica (Hout.) Merr.) terhadap bakteri Streptococcus mutans dan Shigella dysenteriae. J Biotek Medisiana Indones 10: 41–48. doi:10.22435/jbmi.v10i1.5028
Dinos GP, Athanassopoulos CM, Missiri DA, Giannopoulou PC, Vlachogiannis IA, Papadopoulos GE, Papaioannou D, Kalpaxis DL (2016) Chloramphenicol derivatives as antibacterial and anticancer agents: Historic problems and current solutions. Antibiotics 5: 20. doi: 10.3390/antibiotics5020020
Fadliah S, Mu’nisa A, Rachmawaty R (2018) Analisis fitokimia air rebusan daun Kayu Jawa (Lannea coromandelica). J Bionature 19: 73–77. doi:10.35580/bionature.v19i1.7450
Fathoni R, Radiastuti N, Wijayanti F (2017) Identifikasi jenis cendawan pada kelelawar (Ordo Chiroptera) di Kota Tangerang Selatan. J Mikol Indones 1: 28. doi: 10.46638/jmi.v1i1.11
Gauniyal P, Teotia US (2015) Antimicrobial activity of sixteen medicinal plants against oral flora and its efficacy comparison with 2% chlorhexidine. Int J Multidiscip Sci Emerg Res 4:12
Gunjal JN, Patil MS, Chittam KP (2021) Lannea coromandelica: An overview. Int J Pharm Biol Sci Arch 9: 102–107. doi: 10.32553/ijpba.v9i1.181
Indrawati, Sabilu Y, Ompo A (2014) Pengetahuan dan pemanfaatan tumbuhan obat tradisional masyarakat Suku Moronene di Desa Rau-Rau Sulawesi Tenggara. Biowallacea 1: 25–38
Indrayangingsih WOI, Ibrahim N, Anam S (2015) Studi etnofarmasi tumbuhan berkhasiat obat pada Suku Buton di Kecamatan Binongko, Kabupaten Wakatobi, Sulawesi Tenggara. Galen J Pharm 1: 79–84. doi:10.22487/j24428744.2015.v1.i2.6236
Ismail I, Paturusi AAE, Aridani I (2016) Aktivitas antimikroba hasil fraksinasi kortex Kayu Jawa (Lannea coromandelica (Houtt.) Merr.). Biogenesis 4: 122–130. doi: 10.24252/bio.v4i2.2518
Jia M, Chen L, Xin HL, Zheng CJ, Rahman K, Han T, Qin LP (2016) A friendly relationship between endophytic fungi and medicinal plants: A systematic review. Front Microbiol 7: 906. doi: 10.3389/fmicb.2016.00906
JosephStalin D, Babu TS, Kumar S (2013) An investigation on the phytochemistry and in-vitro cytotoxic effects of the aqueous extract of Lannea coromandelica Bark. Pharma Sci Monitor 4: 251–259. Corpus ID: 59124704
Katzung BG, Kruidering-Hall M, Trevor AJ (2019) Katzung & Trevor’s Pharmacology: Examination & Board Review, 12 Edition. McGraw-Hill Education, New York
Khairiah N, Nintasari R (2017) Isolasi dan uji aktivitas antimikroba kapang endofit dari Kayu Ulin (Eusideroxylon zwageri Teijsm & Binn.). J Riset Ind Hasil Hutan 9: 65–74. doi: 10.24111/jrihh.v9i2.3373
Kumala S, Hayatul I (2013) Isolation IPG3-1 and IPG3-3, endophytic fungi from delima (Punica granatum Linn.) twigs and in vitro assessment of their anti microbial activity. Int Res J Pharm 4: 49–53. doi: 10.7897/2230-8407.04611
Oroh SB, Kandou FEF, Pelealu J, Pandiangan D (2015) Uji daya hambat ekstrak metanol Selaginella delicatula dan Diplazium dilatatum terhadap bakteri Staphylococcus aureus dan Escherichia coli. J Ilm Sains 15: 52–58. doi: 10.35799/jis.15.1.2015.8238
Premjanu N, Jaynthy C, Diviya S (2016) Antifungal activity of endophytic fungi isolated from Lannea coromandelica–An insilico approach. Int J Pharm Pharm Sci 8: 207–210. Corpus ID: 88905816
Radiastuti N (2015) Diversity of culturable endophytic fungi in Cinchona calisaya Wedd.: Molecular phylogeny and alkaloid profile. Dissertation, Bogor University
Radiastuti N, Mutea D, Sumarlin LO (2017) Endophytic Colletrotrichum spp. from Cinchona calisaya Wedd. and it’s potential quinine production as antibacterial and antimalaria. AIP Conf Proc 1813: 020022. doi: 10.1063/1.4975960
Roberson RW, Abril M, Blackwell M, Letcher P, McLaughlin DJ, Mouriño-Pérez R, Riquelme M, Uchida M (2010) Hyphal structure, in cellular and molecular biology of filamentous fungi. In: Borkovich KA, Ebbole DJ (Eds). Cellular and Molecular Biology of Filamentous Fungi. ASM Press, Washington DC, pp 8–24. doi: 10.1128/9781555816636.ch2
Rukachaisirikul V, Sommart U, Phongpaichit S, Sakayaroj J, Kirtikara K (2008) Metabolites from the endophytic fungus Phomopsis sp. PSU-D15. Phytochemistry 69: 783?787. doi: 10.1016/j.phytochem.2007.09.006
Strobel G, Daisy B (2003) Bioprospecting for microbial endophytes and their natural products. Microbiol Mol Biol Rev 67: 491–502. doi: 10.1128/MMBR.67.4.491?502.2003
Sudha V, Govindaraj R, Baskar K, Al-Dhabi NA, Duraipandiyan V (2016) Biological properties of endophytic fungi. Braz Arch Biol Technol 59: e16150436. doi: 10.1590/1678-4324-2016150436
Sunny F, Kurniati TH, Hatmanti A (2015) Isolasi dan karakterisasi bakteri penghasil senyawa antibakteri yang berasosiasi dengan karang batu dari perairan Bitung dan spons dari Selat Makassar. Bioma 11: 42-49. doi: 10.21009/bioma11(1).5
Suriani, Muis A (2016) Prospek Bacillus subtilis sebagai agen pengendali hayati patogen tular tanah pada tanaman jagung. J Penelitian Pengembangan Pertanian 35: 37-45. doi: 10.21082/jp3.v35n1.2016.p37-45
Tan RX, Zhou WX (2001) Endophytes: A rich source of functional metabolites. Nat Prod Rep 18: 448?459. doi: 10.1039/ b100918o
Zakiyah A, Radiastuti N, Sumarlin LO (2015) Aktivitas antibakteri kapang endofit dari tanaman kina (Cinchona calisaya Wedd.). AL-Kauniyah J Biol 8: 51–58. doi: 10.15408/kauniyah.v8i2.2690
Zhang B, Liu S, Lei Q, Zhou J, Long C (2020) Phytochemical constituents and pharmacological activities of a traditional medicinal plant, Glochidion eriocarpum (Phyllanthaceae). In: Khasim SM, Long C, Thammasiri K, Lutken H (Eds). Medicinal Plants: Biodiversity, Sustainable Utilization and Conservation. pp 431-441. Springer Nature Singapore, Singapore. doi: 10.1007/978-981-15-1636-8_25
Zheng YK, Qiao XG, Miao CP, Liu K, Chen YW, Xu LH, Zhao LX (2016) Diversity, distribution and biotechnological potential of endophytic fungi. Ann Microbiol 66: 529–542. doi: 10.1007/s13213-015-1153-7
Zulfa I (2016) Isolasi dan uji aktivitas antibakteri kapang endofit akar tanaman Kayu Jawa (Lannea coromandelica (Houtt.) Merr.). Skripsi, UIN Syarif Hidayatullah Jakarta