EKSTRAK KAYU TEGERAN (Cudrania javanensis Trécul) SEBAGAI ANTI JAMUR Peniophora sp.
Main Article Content
Abstract
Jamur Peniophora sp. dapat mendegradasi zat warna alam sehingga warna yang dihasilkan pada kain batik lebih pudar. Penelitian ini dilakukan untuk mempelajari aktivitas anti jamur dari ekstrak kayu tegeran (Cudrania javanensis) terhadap Peniophora sp. yang diisolasi dari ekstrak air kulit mahoni (Swietenia mahagoni). Ekstraksi menggunakan metanol dan air, menghasilkan ekstrak kayu tegeran, yang kemudian konsentrasinya divariasi menjadi 100, 250 dan 500 ppm. Analisis fitokimia ekstrak kayu tegeran dilakukan, dan hasilnya menunjukkan bahwa nilai rata-rata total polisakarida dan terpenoid ekstrak metanol dan air kayu tegeran tidak berbeda nyata (p<0,05). Sedangkan total polifenol kedua ekstrak berbeda nyata (p<0,05). Total polifenol ekstrak metanol kayu tegeran (484,723 mg GAE/g) lebih besar dibandingkan dengan ekstrak air (389,903 mg GAE/g). Hasil menunjukkan bahwa aktivitas anti jamur ekstrak metanol lebih baik dibandingkan dengan ekstrak air kayu tegeran, dan juga lebih tinggi dibandingkan dengan kontrol ketoconazole (100 ppm). Konsentrasi minimum ekstrak metanol kayu tegeran yang dapat menghambat pertumbuhan jamur Peniophora sp. adalah 500 ppm dengan nilai penghambatan (AFA) sebesar 76,30%.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
a). Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Attribution-NonCommercial-ShareAlike 4.0 International that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b). Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
References
Alhakmani F, Kumar S, Khan SA (2013) Estimation of total phenolic content, in-vitro antioxidant and anti-inflammatory activity of flowers of Moringa oleifera. Asian Pac J Trop Biomed 3:623-627. doi: 10.1016/S2221-1691(13)60126-4
Atika V, Salma IR (2017) Kualitas pewarnaan ekstrak kayu tegeran (Cudrania javanensis) pada batik. Majalah Ilmiah Dinamika Kerajinan dan Batik 34:11-18. doi: 10.22322/dkb.v34i1.2642.g2301
Chen L, Zhou Q, Li B, Liu SJ, Dong JX (2015) A new flavonoid from Cudrania cochinchinensis. Nat Prod Res 29:1217-1221. doi: 10.1080/14786419.2014.997234
Chien TV, Anh NT, Thanh NT, Thao TTP, Loc TV, Sung TV (2018) Two new prenylated isoflavones from Maclura cochinchinensis collected in Hoa Binh province Vietnam. Nat Prod Res 33:212-218. doi: 10.1080/14786419.2018.1443096
Chiou WF, Chen CC, Lin IH, Chiu Jh, Chen YJ (2011) 1,3,5-Trihydroxy-4- prenylxanthone represses lipopolysaccharide-induced iNOS expression via impeding posttranslational modification of IRAK-1. Biochem Pharmacol 81:752-760. doi: 10.1016/j.bcp.2010.12.022
Darsih C, Apriyana W, Hayati SN, Rosyida VT, Hernawan, Poeloengasih CD (2017) The elevation effect on water-soluble polysaccharides and DPPH free radical scavenging activity of Ganoderma lucidum K. IOP Conference Series: Mater Sci Eng 172:012010. doi: 10.1088/1757-899X/172/1/012010
EOL (2014) Maclura cochinchinensis (Lour.) Corner. Encyclopedia of life. https://eol.org/pages/2907426. Diakses 02 Januari 2019
Felsenstein J (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783-791. doi: 10.1111/j.1558-5646.1985.tb00420.x
Hiraishi A, Kamagata Y, Nakamura K (1995) Polymerase chain reaction amplification and restriction fragment length polymorphism analysis of 16S rRNA genes from methanogens. J Ferment Bioeng 79:523-529. doi: 10.1016/0922-338X(95)94742-A
Kalidindi N, Thimmaiah NV, Jagadeesh NV, Nandeep R, Swetha S, Kalidindi B (2015) Antifungal and antioxidant activities of organic and aqueous extracts of Annona squamosa Linn. leaves. J Food Drug Anal 23:795-802. doi: 10.1016/j.jfda.2015.04.012
Kalita M, Sarmah GK, Bora MN, Das B, Kotoky J (2009) Molecular and crystal structure of an isoflavonoid, 5,7,4׳-trihydroxy-6,3’-diprenylisoflavone from Cudrania javanensis. Indian J Chem Sec B 48:1324-1328. doi: 10.1002/chin.200947195
Kongkiatpaiboon S, Tungsukruthai P, Sriyakool K, Pansuksan K, Tunsirikongkon A, Pandith H (2017) Determination of morin in Maclura cochinchinensis heartwood by HPLC. J Chromatogr Sci 55:346-350. doi: 10.1093/chromsci/bmw191
Kumar S, Nei M, Dudley J, Tamura K (2008) MEGA:A biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9:299-306. doi: 10.1093/bib/bbn017
Kummee S, Intaraksa N (2008) Antimicrobial activity of Desmos chinensis leaf and Maclura cochinchinensis wood extracts. Songklanakarin. J Sci Technol 30:635-639
Leakaya N, Sato VH, Chewchinda S (2018) Antioxidant activity, total phenolic, total flavonoid content and HPTLC analysis of morin in Maclura cochinchinensis heartwood extract. Thai J Pharm Sci 42:27-31
Lin M-S, Yu Z-R, Wang B-J, Wang C-C, Weng Y-M, Koo M (2015) Bioactive constituent characterization and antioxidant activity of Ganoderma lucidum extract fractionated by supercritical carbon dioxide. Sains Malays 44:1685-1691
Liu ZP, Wei WX, Zhou M, Gan CF, Liu S (2013) A new prenylated xanthone from root barks of Cudrania cochinchinensis. Chin Herbal Med 5:101-103. doi: 10.3969/j.issn.1674-6348.2013.02.003
Moorthy KK, Subramaniam P, Senguttuvan J (2013) In vitro antifungal activity of various extracts of leaf and stem parts of Solena amplexicaulis (Lam.) Gandhi. Int J Pharm Pharm Sci 5:745-747
Mori M, Aoyama M, Doi S, Kanetoshi A, Hayashi T (1997) Antifungal activity of bark extracts of deciduous trees. Holz als Roh-und Werkstoff 55:130-132
MycoCosm (2019) Peniophora sp. The Fungal Genomics Resource. https://genome.jgi.doe.gov/Lopni1/Lopni1.home.html. Diakses 10 juni 2019
Nakashima KI, Ogiwara T, Hirai T, Tanaka T, Murata H, Kaburagi K, Fujii-Kuriyama Y, Hayashi H, Inoue M (2017) Gerontoxanthone B from Maclura cochinchinensis var. gerontogea exhibits anti-inflammatory potential as an aryl hydrocarbon receptor agonist. Bioorg Med Chem 25:4253-4258. doi: 10.1016/j.bmc.2017.05.047
Paringkarn S, Sato VH, Parichatikanond W, Chewchinda S (2018) The study of hypouricemic effect of Maclura cochinchinensis heartwood extract. 34th International Annual Meeting in Pharmaceutical Sciences and 2nd CU FPhS-RIKEN CDB Symposium. Thai J Pharm Sci 42:98-101
Sarmah GK, Sharma RK (2010) Isolation and identification of a biologically active isoflavonoid from Cudrania javanensis. J Phytol 2:47-49
Septhum C, Rattanaphani S, Bremner JB, Rattanaphani V (2009) An adsorption study of alum-morin dyeing onto silk yarn. Fiber Polym 10:481-487
Shankar S, Shikha N (2012) Laccase production and enzymatic modification of lignin by a novel Peniophora sp. Appl Biochem Biotechnol 166:1082-1094. doi: 10.1007/s12010-011-9496-4
Shankar S, Shikha N (2015) Effect of metal ions and redox mediators on decolorization of synthetic dyes by crude laccase from a novel white rot fungus Peniophora sp. (NFCCI-2131). Appl Biochem Biotechnol 175:635-647. doi: 10.1007/s12010-014-1279-2
Swargiary A, Ronghang B (2013) Screening of phytochemical constituents, antioxidant and antibacterial properties of methanolic bark extracts of Maclura cochinchinensis (Lour) corner. Int J Pharm Biol Sci 4:449-459
Wang L, Kuang L, Pan X, Liu J, Wang Q, Du B, Li D, Luo J, Liu M, Hou A, Qian M (2010) Isoalvaxanthone inhibitis colon cancer cell proliferation, migration and invasion through inactivating Rac1 and AP-1. Int J Cancer 127:1220-1229. doi: 10.1002/ijc.25119
Wang C-J, Chen C-C, Tsay H-J, Chiang F-Y, Wu M-F, Shiao Y-J (2013) Cudrania cochinchinensis attenuates amyloid β protein-mediated microglial activation and promotes glia-related clearance of amyloid β protein. J Biomed Sci 20:55. doi: 10.1186/1423-0127-20-55
White TJ, Bruns TD, Lee SB, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (Eds). PCR protocols and Applications: A laboratory manual Pp 315-322. Academic Press, San Diego
Zheng ZP, Zhu Q, Fan CL, Tan HY, Wang M (2011) Phenolic tyrosinase inhibitors from the stems of Cudrania cochinchinensis. Food Funct 2:259-264. doi: 10.1039/c1fo10033e
Zhou Q, Chen L, Yin H-J, Li B, Tian Y, Chen H-W, Xiao Y-H, He X-H, Zeng Y-L, Dong J-X (2014) Two new flavonols from Cudrania cochinchinensis. J Asian Nat Prod Res 16:976-981. doi: 10.1080/10286020.2014.923843