EKSTRAKSI DAN IDENTIFIKASI METABOLIT SEKUNDER DARI ISOLAT AL6 SERTA POTENSINYA SEBAGAI ANTIBAKTERI TERHADAP Escherichia coli
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Abstract
Metabolit sekunder berupa antibiotik dapat diproduksi oleh bakteri rizosfer. Isolat bakteri AL6, salah satu isolat bakteri dari rizosfer Saccarum officinarum L., diketahui dapat menghasilkan senyawa antibiotik. Penelitian ini bertujuan mengetahui aktivitas antibiotik dari ekstrak etil asetat antibiotik AL6 yang dihasilkan isolat bakteri AL6, menganalisis kadar hambat minimum (KHM), serta kemiripan zat aktif menggunakan GCMS. Ekstrak etil asetat yang diperoleh diuji KHM-nya pada konsentrasi 1,25%, 2,5%, 5,0%, 10,0%, 20%, dan 40%. Deteksi bercak yang berpotensi sebagai antibiotik dilakukan menggunakan kromatografi lapis tipis (KLT) bioautografi. Senyawa yang berperan dalam aktivitas antibiotik dianalisis menggunakan GCMS. Kadar hambat minimal yang diperoleh mencapai 2,5%. Hasil uji KLT bioautografi memperlihatkan bercak aktif sebagai antibiotik terhadap Escherichia coli pada Rf 0,94. Komponen senyawa yang terdeteksi menggunakan GCMS dan diduga sebagai antibiotik antara lain chloroform; ethane, 1,1-dimethoxy-(CAS) dimethyl acetal; dan 1,3-dioxolane, 2-methoxymethyl-2,4,5-trimethyl.
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