PERBANDINGAN AKTIVITAS ANTIBAKTERI EKSTRAK KASAR RESIN DAN DAUN GAHARU (Gyrinops versteegii)
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Abstract
Penelitian ini bertujuan untuk membandingkan aktivitas antibakteri daun gaharu dan resin gaharu G. versteegii menggunakan beberapa pelarut pengekstrak dan konsentrasi ekstrak yang berbeda. Bahan daun dan resin gaharu dikeringkan dan dicacah, kemudian diekstrak menggunakan metode maserasi. Uji aplikasi ekstrak pada Staphylococcus aureus ATCC25923 dilakukan menggunakan rancangan faktorial, yaitu faktor pertama bahan ekstrak (resin, daun), faktor kedua pelarut pengekstrak (etanol, metanol), dan faktor ketiga konsentrasi ekstrak (0,25; 0,5; dan 1 g mL–1). Data zona hambat terhadap bakteri uji dianalisis menggunakan ANOVA, BNJ dan standard error. Rerata zona hambat ekstrak daun gaharu (7 mm) lebih besar secara signifikan dibandingkan dengan rerata zona hambat ekstrak resin (6,5 mm). Faktor pelarut pengekstrak dan konsentrasi ekstrak tidak berpengaruh signifikan terhadap zona hambat. Pelarut pengekstrak dan konsentrasi ekstrak memberikan pengaruh interaksi berbeda-beda terhadap zona hambat. Dapat disimpulkan bahwa bahan ekstrak daun gaharu memiliki aktivitas antibakteri lebih bagus dibandingkan dengan ekstrak resin gaharu dengan konsentrasi ekstrak efisien sebesar 0,25 g mL
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