ANALISIS HASIL FRAKSINASI PROTEASE DAN LIPASE YANG BERASAL DARI SALURAN PENCERNAAN UDANG VANAME (Litopenaeus vannamei)
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Abstract
Udang vaname merupakan komoditi perikanan dengan nilai konsumsi yang tinggi, sehingga berdampak pula dengan tingginya limbah udang yang berupa kepala dan kulit. Saluran pencernaan yang terhubung dengan kepala udang vaname (Litopenaeus vannamei) mengandung enzim pencernaan, diantaranya protease dan lipase. Penelitian ini bertujuan untuk mendapatkan fraksi protein yang memiliki aktivitas protease dan lipase tertinggi. Metode pemisahan yang dilakukan adalah sentrifugasi dilanjutkan dengan pengendapan menggunakan garam ammonium sulfat dan dialisis. Dialisat dimurnikan dengan kromatografi filtrasi gel pada retensi volume sebanyak 10 tetes tiap tabung. Aktivitas enzim proteolitik dan lipolitik fraksi diukur menggunakan spektrofotometer. Hasil menunjukkan bahwa fraksi 102 memiliki nilai aktivitas protease tertinggi sebesar 96,3924 U mL–1, sedangkan fraksi 100 memiliki aktivitas lipase tertinggi sebesar 531,07 U mL–1. Penelitian ini menunjukkan bahwa pada saluran pencernaan udang vaname terdapat aktivitas protease dan lipase yang meningkat seiring dengan tingkat kemurniannya.
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