DAYA REGENERASI KALUS DAN TUNAS IN VITRO PADI VARIETAS TARABAS PADA BERBAGAI KONSENTRASI 2,4-D

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Wahyu Indra Duwi Fanata
Dalliyah Hadrotul Qudsiyah

Abstract

Badan Penelitian dan Pengembangan Pertanian serta Pemerintah Provinsi Jawa Barat mengembangkan varietas unggul baru dengan standar padi Japonica yaitu varietas Tarabas. Namun, kesetaraan varietas Tarabas dengan varietas Japonica asli dalam kemampuan embriogenesis somatik belum dilaporkan. Penelitian ini membandingkan respons kultur jaringan antara beras Tarabas dan padi Japonica varietas Hwayoung. Induksi kalus dari embrio matang dengan beberapa konsentrasi 2,4-D menunjukkan respons pembentukan kalus yang sama pada kedua varietas padi. Kalus yang diinduksi 1 ppm 2,4-D menunjukkan laju pembentukan tunas yang lebih tinggi. Di sisi lain, kalus Hwayoung yang diinduksi konsentrasi 2,4-D yang lebih tinggi menunjukkan penghambatan dalam pembentukan tunas. Di lain pihak, pembentukan kalus padi Tarabas tidak terpengaruh oleh peningkatan konsentrasi 2,4-D dan mampu menunjukkan 100% laju regenerasi tanaman pada minggu keempat di media regenerasi walaupun pertumbuhan tunas tidak secepat pada perlakuan 1 ppm 2,4-D. Karena itu, hasil penelitian ini menunjukkan bahwa varietas Tarabas memiliki kapasitas embriogenesis somatik yang setara dengan padi japonica dan padi Tarabas mempunyai potensi sebagai obyek penelitian di bidang bioteknologi.

Article Details

How to Cite
Wahyu Indra Duwi Fanata, & Dalliyah Hadrotul Qudsiyah. (2021). DAYA REGENERASI KALUS DAN TUNAS IN VITRO PADI VARIETAS TARABAS PADA BERBAGAI KONSENTRASI 2,4-D. Jurnal Bioteknologi Dan Biosains Indonesia, 7(2), 250–258. https://doi.org/10.29122/jbbi.v7i2.4404
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References

Ahmad P, Ashraf M, Younis M, Hu X, Kumar A, Akram NA, Al-Qurainy F (2012) Role of transgenic plants in agriculture and biopharming. Biotechnol Adv 30: 524-540. doi: 10.1016/j.biotechadv.2011.09.006

Ali S, Qing-Zhong X, Xian-Yin Z (2004) Assessment of various factors involved in the tissue culture system of rice. Rice Sci 11: 345-349. Corpus ID: 54818359

Azizah KA, Restanto DP, Sugiharto B (2017) Enhancement of regeneration efficiency through callus induction media using 2,4-dicholorophenoxyacetic acid in indica rice (Oryza sativa L. var. Ciherang). J Ilmu Dasar 18: 91-98. doi: 10.19184/jid.v18i2.2592

Ben Amer IM, Börner A (1997) The relationship between green spot initiation and plantlet regeneration of wheat callus grown under short-term conditions. Plant Cell Tiss Organ Cult 50: 67-69. doi: 10.1023/A:1005855912655

Binte Mostafiz S, Wagiran A (2018) Efficient callus induction and regeneration in selected indica rice. Agronomy 8: 77-94. doi: 10.3390/agronomy8050077

Cortleven A, Schmülling T (2015) Regulation of chloroplast development and function by cytokinin. J Exp Bot 66: 4999-5013 doi: 10.1093/jxb/erv132

Fanata WID, Lee KH, Son BH, Yoo JY, Harmoko R, Ko KS, Ramasamy NK, Kim KH, Oh DB, Jung HS, Kim JY, Lee SY, Lee KO (2013) N-glycan maturation is crucial for cytokinin-mediated development and cellulose synthesis in Oryza sativa. Plant J 73: 966-979. doi: 10.1111/tpj.12087

Feng F, Li Y, Qin X, Liao Y, Siddique KHM (2017) Changes in rice grain quality of indica and japonica type varieties released in China from 2000 to 2014. Front. Plant Sci 8: 1863. doi: 10.3389/fpls.2017.01863

Fraiture MA, Roosens NHC, Taverniers I, De Loose M, Deforce D, Herman P (2016) Biotech rice: Current developments and future detection challenges in food and feed chain. Trends Food Sci Technol 52: 66-79. doi: 10.1016/j.tifs.2016.03.011

He Z, Xia X, Peng S, Lumpkin TA (2014) Meeting demands for increased cereal production in China. J Cereal Sci 59: 235-244. doi: 10.1016/j.jcs.2013.07.012

Hiei Y, Komari T (2008) Agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed. Nat Protoc 3: 824-834. doi: 10.1038/nprot.2008.46

Hoque ME, Ali MS, Karim NH (2007) Embryogenic callus induction and regeneration of elite Bangladeshi indica rice cultivars. Plant Tiss Cult Biotechnol 17: 65-70. doi: 10.3329/ptcb.v17i1.1122

Huang WL, Lee CH, Chen YR (2012) Levels of endogenous abscisic acid and indole-3-acetic acid influence shoot organogenesis in callus cultures of rice subjected to osmotic stress. Plant Cell Tiss Organ Cult 108: 257-263. doi: 10.1007/s11240-011-0038-0

Joyia FA, Khan MS (2013) Scutellum-derived callus-based efficient and reproducible regeneration system for elite varieties of indica rice in Pakistan. Int J Agric Biol (Pakistan) 15: 27-33

Lee ST, Huang WL (2014) Osmotic stress stimulates shoot organogenesis in callus of rice (Oryza sativa L.) via auxin signaling and carbohydrate metabolism regulation. Plant Growth Regul 73: 193-204. doi: 10.1007/s10725-013-9880-x

Libin A, King PJ, Ong KH, Chubo JK, Sipen P (2012) Callus induction and plant regeneration of Sarawak rice (Oryza sativa L.) variety Biris. Afr J Agric Res 7: 4260-4265. doi: 10.5897/ajar12.587

Mitsuoka K, Honnda H, Xing XH, Unno H (1994) Effect of intracellular 2,4-D concentration on plantlet regeneration of rice (Oryza sauva L.) callus. Appl Microbiol Biotechnol 42: 364-366. doi: 10.1007/BF00902743

Mohammed S, Samad AA, Rahmat Z (2019) Agrobacterium-mediated transformation of rice: Constraints and possible solutions. Rice Sci 26: 133-146. doi: 10.1016/j.rsci.2019.04.001

Rahman ZA, Ramli A, Hosni H, Kamaruzaman R, Seman ZA, Othman AN, Zainal Z, Uddain J, Subramaniam S (2015) Efficient plant regeneration of Malaysian aromatic rice (Oryza sativa L.) through somatic embryogenesis. Emirates J Food Agric 27: 857-863. doi: 10.9755/ejfa.2015-07-535

Sah SK, Kaur A (2013) Genotype independent tissue culture base line for high regeneration of japonica and indica rice. Res J Biotechnol 8: 96-101

Sah SK, Kaur A, Sandhu JS (2014a) High frequency embryogenic callus induction and whole plant regeneration in japonica rice Cv. Kitaake. J Rice Res 2: 125. doi: 10.4172/jrr.1000125

Sah SK, Kaur A, Kaur G, Cheema GS (2014b) Genetic transformation of rice: Problems, progress and prospects. J Rice Res 3: 132. doi: 10.4172/2375-4338.1000132

Sahoo KK, Tripathi AK, Pareek A, Sopory SK, Singla-Pareek SL (2011) An improved protocol for efficient transformation and regeneration of diverse indica rice cultivars. Plant Methods 7: 49. doi: 10.1186/1746-4811-7-49

Shahsavari E, Maheran AA, Akmar ASN, Hanafi MM (2010) The effect of plant growth regulators on optimization of tissue culture system in Malaysian upland rice. Afr J Biotechnol 9: 2089-2094. doi: 10.5897/AJB10.1925

Sidorov VA (2013) Plant tissue culture in biotechnology: Recent advances in transformation through somatic embryogenesis. Biotechnol Acta 6: 118-131. doi: 0.15407/biotech6.04.118

Wani SH, Sanghera GS, Gosal SS (2011) An efficient and reproducible method for regeneration of whole plants from mature seeds of a high yielding Indica rice (Oryza sativa L.) variety PAU 201. N Biotechnol 28: 418-422. doi: 10.1016/j.nbt.2011.02.006

Zhao W, Zheng S, Ling HQ (2011) An efficient regeneration system and Agrobacterium-mediated transformation of Chinese upland rice cultivar Handao297. Plant Cell Tiss Organ Cult 106: 475-483. doi: 10.1007/s11240-011-9946-2