2026-10-20T22:41:13Z https://ejournal.brin.go.id/annales/oai

oai:ejournal.brin.go.id:article/2921 2026-10-20T13:37:07Z annales:ART

The Screening of Antibacterial Activity of Selenium Nanoparticles Produced by Indonesian Marine Actinomycetes Ratnakomala, Shanti Atikana, Akhirta Sari, Nurul Fitri Ridwan, Roni Fahrurrozi, Fahrurrozi Lisdiyanti, Puspita actinomycetes selenium nanparticles antimicrobial activities Actinomycetes strains can reduce SeO3-2 (selenite) and (or) SeO4-2 (selenate) to Se0, which is less toxic by forming selenium nanoparticles (SeNPs). This study aimed to screen the actinomycetes strains capable of biosynthesizing and producing SeNPs with antibacterial activity. This study showed that the antibacterial properties of SeNPs were synthesized biologically by the three strains of actinomycetes, e.g., the strains BLH 5-21, BLH 5-22, and BLH 12-1. The nanoparticles synthesized by the three actinomycetes strains were active against the pathogenic tested bacteria: Bacillus subtilis BTCC B-612, Staphylococcus aureus BTCC B-611, Micrococcus luteus BTCC B-552, Escherichia coli BTCC B-609, and Candida albicans Y-033. The PSA and SEM images showed that the selenium nanoparticles from strain BLH 12-1 had a spherical shape, and their mean diameter was between 64-110 nm, with an average size of 98 nm. The biogenic selenium nanoparticles have exhibited promising application prospects in medicine, biosensors, and environmental remediation. These microorganisms might be explored as the potential of microbial nano factory for synthesizing metal nanoparticles. National Research and Innovation Agency (BRIN) 2025-12-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/2921 10.55981/ann.bogor.2025.2921 Annales Bogorienses; Vol. 29 No. 2 (2025): Annales Bogorienses; 11-19 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/2921/13278 Copyright (c) 2025 Shanti Ratnakomala, Akhirta Atikana, Nurul Fitri Sari, Roni Ridwan, Fahrurrozi Fahrurrozi, Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/2943 2026-07-30T15:29:47Z annales:ART

oai:ejournal.brin.go.id:article/4149 2026-10-19T03:56:40Z annales:ART

ANTI-DIABETIC EFFECT OF 70% ETHANOL EXTRACT OF Anredera cordifolia (Teen) Stennis (BINAHONG) LEAVES ON GLUCOSE TRANSPORTER 4 (GLUT4) AND PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMA (PPAR-γ) GENES EXPRESSION IN 3T3-L1 ADIPOCYTE CELLS Asri Sulfianti Anredera cordifolia anti-diabetic expression GLUT4 PPAR-γ The evidence of Anredera cordifolia (Binahong) as anti-diabetic at molecular stage has not been elucidated. Present study, we investigate the antidiabetic potential of Binahong by evaluating its effect on two diabetic gene markers expression, Translocation of Glucose Transporter 4 (GLUT4) and Peroxisome Proliferator-Activated Receptor Gamma (PPAR-γ) in 3T3-L1 adipocytes. The investigation has been done by semi quantitative real time PCR. Our finding shown, the GLUT4 MRNA levels were dose dependent excess in adipose treated by 6.25-12.5 mg/ml of 70% Binahong ethanol extract. Though, at 50 mg/ml dose, the GLUT4 expression was up down to 1.2 fold. In addition, the pattern of PPAR-γ MRNA levels in 3T3-L1 were almost same. Every Binahong extracts gave increment on PPAR-γ MRNA levels except 3.125, 6.25, and 50 mg/ml doses. In undifferentiated adipocytes, GLUT4 and PPAR-γ mRNA were barely expressed. The up regulation of those MRNA levels probably occurred because of Binahong has an active compound. However, it is not yet known which compounds who regulate the expression and induces translocation of GLUT4. We hypothesis that it acts as ligand for the PPAR-γ transcriptional factor which activates the GLUT4 transcription and plays orchestra in glucose homeostasis at molecular level.   National Research and Innovation Agency (BRIN) 2022-06-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article https://ejournal.brin.go.id/annales/article/view/4149 10.14203/ann.bogor.2021.v25.n1.1-7 Annales Bogorienses; Vol. 25 No. 1 (2021); 1-7 2407-7518 0517-8452 Copyright (c) 2022 Asri Sulfianti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4150 2026-10-19T03:56:40Z annales:ART

COMPARATIVE ANALYSIS OF VIRULENCE FACTOR BETWEEN DIFFUSIBLE SIGNAL FACTOR BIOSENSOR AND INDONESIAN Xanthomonas oryzae pv oryzae Fatimah Iqbal Kusnandarsyah Apon Zaenal Mustopa Nurlaili Ekawati Cellulose Diffusible signal factor Exopolysaccharide Xanthomonas oryzae pv. Oryzae (Xoo) is the causal agent of bacterial blight in rice (Oryza sativa L.). In the understanding of virulence determinants of three Indonesian Xoo, extracellular polysaccharide (EPS) production, cellulose and diffusible signal factor (DSF) were identified. DSF signaling system in Xoo is involved in the regulation of virulence factor production. In this study the comparative analysis was conducted with the DSF biosensor isolate (Xoo-dsf). The results showed that high yield of EPS and cellulose activity revealed from isolate Xoo1130 and Xoo1122 compare to Xoo93229 while Xoo-dsf giving similar result with Xoo1130. The comparative analysis of Xoo1130 and Xoo-dsf showed that Xoo1130 produced three DSF-family signals (DSF, BDSF and CDSF) similar to the DSF biosensor isolate. It assumed that Xoo1130 uses a similar mechanism in Xoo-dsf to autoregulate the biosynthesis of the DSF-family signals. National Research and Innovation Agency (BRIN) 2022-06-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4150 10.14203/ann.bogor.2021.v25.n1.8-14 Annales Bogorienses; Vol. 25 No. 1 (2021); 8-14 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4150/2953 Copyright (c) 2022 Fatimah, Iqbal Kusnandarsyah, Apon Zaenal Mustopa, Nurlaili Ekawati https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4156 2026-10-19T03:56:40Z annales:ART

Fermentation Tofu Using Consortium Lactic Acid Bacteria Isolated from Whey Tofu as Biocoagulant and Bioperservation Siti Eka Yulianti Dea Indriani Astuti Tofu is a traditional food that has high protein content and is prone to spoilage. A fermentation process with consortium lactic acid bacteria (LAB) that can produce bacteriocin has is carried out to get tofu that is more durable with better taste.  The Bacteria which are used in this study are Lactobacillus fermentum, Leuconostoc mesenteroides, and Lactobacillus plantarum.  L. fermentum is isolated from whey tofu. The antibacterial ability of each bacteria is measured by bacteriocins assay after incubation at 0 hours; 4 hours; and 8 hours in a row are L. fermentum 0; 2506.429 AU/mL; 1502.679 AU/mL;  L. mesenteroides 0 AU/mL; 2506.43 AU/mL; 2939.44 AU/mL; L. plantarum 760.39 AU/mL; 3341.25 AU/mL; 3889.29 AU/mL. Ratio inoculum bacteria (a:b:c) which are used are 2:1:1, 1:1:2, and 1:1:1 by using percentage of inoculum 10%. The Optimum inoculum age of these bacterium is 6 hours with µmax each bacteria in a row is 0.04 h-1, 0.02 h-1, dan 0.02 h-1. The fermentation is processed in 8 hours at 37ᵒC of temperature without agitation. From this optimation, the highest bacteriocin activity in tofu curd is 1767.857 AU/mL, which is in a ratio of 1:1:2 at the 4th hour. National Research and Innovation Agency (BRIN) 2022-06-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4156 10.14203/ann.bogor.2021.v25.n1.15-27 Annales Bogorienses; Vol. 25 No. 1 (2021); 15-27 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4156/2954 Copyright (c) 2022 Siti Eka Yulianti, Dea Indriani Astuti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4157 2026-10-19T03:56:40Z annales:ART

Acid-producing Bacteria and Yeasts Isolated from Arabica Coffee Plantations in Central Java and East Java, Indonesia Dinihari Indah Kusumawati Toto Iswanto Puspita Lisdiyanti Java coffee Lactobacillus Pediococcus pentosaceus Leuconostoc pseudomesenteroides Candida tropicalis The converting process of coffee cherries to green coffee beans was conducted through the fermentation process using lactic acid bacteria (LAB) and yeasts to degrade coffee's mucilage. This present study aims to isolate acid producing bacteria and yeasts from three type of samples obtained from Arabica Coffee Plantations in Central Java and East Java, Indonesia. A total of 31 bacteria and 15 yeasts were obtained from this study. From 31 bacteria, 6 strains were identified as Lactobacillus sp. isolated from all samples; 15 strains were identified as P. pentosaceus; 4 strains as L. pseudomesenteroides; 1 strain as K. quasipneumoniae; and 5 strains as A. tropicalis from exocarp of coffee fruit. While from 15 yeast, 8 strains were identified as C. tropicalis isolated from soil; 2 strains as P. kudriavzevii from Luwak feces, and 5 strains as C. fabianii from exocarp of coffee fruit. A total of 25 strains of lactic acid bacteria can release lactic acid bacteria on MRS agar supplemented with 0.5% CaCO3, but 1 strain of K. quasipneumoniae and 5 strains of A. tropicalis produced acid weakly. Four strains of C. tropicalis showed the ability to form thin clear zone formation contrariwise with four other strains. This study adduced that waste materials and soil from the plantation coffee’s area are potential sources to obtaining acid-producing bacteria which is utilized in fermentation process of coffee cherries.  National Research and Innovation Agency (BRIN) 2022-06-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4157 10.14203/ann.bogor.2021.v25.n1.28-40 Annales Bogorienses; Vol. 25 No. 1 (2021); 28-40 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4157/2955 Copyright (c) 2022 Dinihari Indah Kusumawati, Toto Iswanto, Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4158 2026-10-19T03:56:40Z annales:ART

POTENTIAL OF GROWTH HORMONES IAA OF ENDOPHITIC MICROBES FROM NEEM PLANTS FOR CAPITAL OF INDUSTRIAL OPPORTUNITY Sylvia J R Lekatompessy Nuriyanah Liseu Nurjanah Eleazar Handoyo Rumella Simarmata Tiwit Widowati neem IAA hormone industrial opportunity endophytic microbes Indonesia has a tropical climate that is rich in diversity of microorganisms with useful potential and has important values that can be developed in the fields of industry, health and agriculture. Neem is a plant commonly used as an insecticide for pest control. Biotechnology continues to develop in exploiting the unique and interesting potential of endophytic microbes to obtain useful bioactive compounds extracted from plants, without having to take large amounts of plant biomass. This study aims to explore other potentials of endophytic microbes from neem plants, namely as a producer of IAA growth hormone to support plant growth. The results of the test of growth hormone-producing microbes, from 19 isolates of endophytic bacteria tested, obtained 3 isolates of IAA hormone-producing bacteria. 36 isolates of endophytic fungi were tested, 18 isolates of endophytic fungi were positive for IAA hormone production. One of the isolates of endophytic fungi that produces the highest IAA hormone, has been identified molecularly and the result is Colletotrichum gloeosporioides. The potential value derived from the endophytic microbes of neem plants can be developed into biotechnology products that have high commercial value by utilizing potential genes to support plant growth. National Research and Innovation Agency (BRIN) 2022-06-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4158 10.14203/ann.bogor.2021.v25.n1.41-54 Annales Bogorienses; Vol. 25 No. 1 (2021); 41-54 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4158/2956 Copyright (c) 2022 Sylvia J R Lekatompessy, Nuriyanah, Liseu Nurjanah, Eleazar Handoyo, Rumella Simarmata, Tiwit Widowati https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4281 2026-10-19T03:56:40Z annales:ART

Synthesis and Characterization of Alginate-Cellulose Xanthate Beads from Corn Stalk with NaCl As Porogen Rohmatullah, Wahyu Adhi Putra Yulianti, Eny Khoiroh, Lilik Miftahul Mahmudah, Rif'atul cornstalk cellulose beads Alginate porogen cross-linked In this study, the synthesis of porous beads from corn stalks was carried out. The cellulose extracted from corn stalks was converted into cellulose xanthate and combined with alginate to form porous alginate-cellulose xanthate beads by the ionic gelation method. This study attempted to use sodium chloride (NaCl) as a porogen and zinc acetate as a crosslinker. Beads were characterized to determine the porosity, swelling properties, and functional groups using Fourier Transform Infra-Red (FTIR). The geometry of beads was analyzed by optical microscopy, and its surface morphology was analyzed by Scanning Electron Microscopy-Energy Dispersive X-ray (SEM-EDX). The results showed that corn stalks as agricultural waste material could be used to synthesize porous beads material. The swelling and porosity of beads increased with increasing concentration of NaCl. The presence of porogen has increased beads formation. The results demonstrate the crosslinks between zinc acetate and alginate were successfully characterized using FTIR. NaCl concentration of 9.5% resulted in the highest swelling properties (52.80%) and porosity (81.4%) of the beads. National Research and Innovation Agency (BRIN) 2021-01-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4281 10.14203/ann.bogor.2020.v24.n2.59-65 Annales Bogorienses; Vol. 24 No. 2 (2020): Annales Bogorienses; 59-65 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4281/3097 Copyright (c) 2021 Wahyu Adhi Putra Rohmatullah, Eny Yulianti, Lilik Miftahul Khoiroh, Rif'atul Mahmudah https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4282 2026-10-19T03:56:40Z annales:ART

Study on Endophytic Fungi Associated with Moringa oleifera Lam. Collected from Lombok Island, West Nusa Tenggara Ramadhani, Indriati Rohadi, Hasnadiazahra Yuliani, Yeni Ilyas, Muhammad endophytic fungi identification isolation ITS rDNA Moringa oleifera Lam An assemblage of endophytic fungi was isolated from Moringa oleifera Lam. collected from Lombok island, West Nusa Tenggara Province, Indonesia. Fungal endophytes were isolated using surface sterilization methods with slight modification. Forty-six selected endophytic fungal strains were isolated from the leaves, petioles, and stems of M. oleifera. The fungal strains identification through morphological observation and ITS rDNA-based molecular analysis showed that fungal endophytes were associated with host plants belonging to the taxa Alternaria, Cladosporium, Colletotrichum (Glomerella), Corynespora, Curvularia (Cochliobolus), Fusarium, Mucor, Ochrocladosporium, Phomopsis (Diaporthe), and Trametes. In this study, endophytic Phomopsis dominating the obtained strains, whereas 26% (12/46) strains were isolated from host plant samples. National Research and Innovation Agency (BRIN) 2021-01-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4282 10.14203/ann.bogor.2020.v24.n2.66-73 Annales Bogorienses; Vol. 24 No. 2 (2020): Annales Bogorienses; 66-73 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4282/5904 Copyright (c) 2021 Indriati Ramadhani, Hasnadiazahra Rohadi, Yeni Yuliani, Muhammad Ilyas https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4284 2026-10-19T03:56:40Z annales:ART

The Purification of Rennin-Like Protease from Lactobacillus paracasei Isolated from Ettawa Goat Milk Putranto, Wendry Setiyadi Mustopa, Apon Zaenal Kusumawati, Arizah Prastyowati, Anika lactobacillus rennin like protease purification There is a protease produced by bateria that has characteristics similar to rennin from a calf.  Rennin has the ability to clot casein in milk. Rennin-like protease (RLP) is produced by bacteria extracellularly. Lactic Acid Bacteria (LAB) have the potential to be developed for RLP production because they are safe and non-pathogenic bacteria. Rennin is needed in the process of milk coagulation to subsequently obtain a curd in the process of making cheese. In this study, the LAB isolated from Ettawa goat milk (isolate 2.12) which produced RLP was 99% identical to Lactobacillus paracasei based on 16S rRNA gene sequence analysis. The purification of the RLP L. paracasei 2.12 with 60% ammonium sulfate deposition, dialysis, and filtration gel chromatography Sephadex G-50 showed a single 38 kDa protein band with SMCA/SPA was 4.48 higher than that of the calf rennet with a ratio value of 1, therefore in this study, RLP L. paracasei 2.12  was developed as an alternative to renin in cheese making. National Research and Innovation Agency (BRIN) 2021-01-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4284 10.14203/ann.bogor.2020.v24.n2.74-80 Annales Bogorienses; Vol. 24 No. 2 (2020): Annales Bogorienses; 74-80 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4284/3098 Copyright (c) 2021 Wendry Setiyadi Putranto, Apon Zaenal Mustopa, Arizah Kusumawati, Anika Prastyowati https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4285 2026-10-19T03:56:40Z annales:ART

Inhibitory Potential of Black Seed (Nigella sativa L.) Bioactive Compounds Towards Main Protease of SARS-CoV-2: In Silico Study Ferdian, Pamungkas Rizki Elfirta, Rizki Rabeca Emilia, Qori Ikhwani, Azra Zahrah Nadhirah COVID-19 Nigella sativa L molecular docking SARS-CoV-2 COVID-19, caused by SARS-CoV-2, has become a massive worldwide concern of the 21st century. One potential strategy to block the biochemical pathway of SARS-CoV-2 was by inhibiting the main protease (Mpro), which is a key enzyme on viral replication. Black seed (Nigella sativa L.) has a long history for its use as a traditional medicine. Therefore, we hypothesised that the black seed contains numerous active compounds that could potentially confer inhibitory activity against SARS-CoV-2 viral Mpro. In this study, 24 active compounds from black seed were tested. Compounds were screened using Lipinski's Rules and admetSAR, then docked to viral Mpro 7BQY by AutoDockTools-1.5.6 and AutoDock Vina using a site directed docking approach resulting in affinity energy (∆G) and binding data. We found that the most potential active compound of N. sativa is 3-[(4-Methylphenyl)sulfanyl]-1,3-diphenyl-1-propanone, since its affinity energy was -7.6 kCal.mol-1. Its similarity to N3 inhibitor based on Ligplot analysis and DS were 86.7% and 76.19%, respectively, and the occupancy on binding site based on Ligplot analysis and DS were 90.91% and 81.82%, respectively. These findings can be used as a starting point for further investigation using in vitro and in vivo studies.  National Research and Innovation Agency (BRIN) 2021-01-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4285 10.14203/ann.bogor.2020.v24.n2.81-94 Annales Bogorienses; Vol. 24 No. 2 (2020): Annales Bogorienses; 81-94 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4285/3099 Copyright (c) 2021 Pamungkas Rizki Ferdian, Rizki Rabeca Elfirta, Qori Emilia, Azra Zahrah Nadhirah Ikhwani https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4286 2026-10-19T03:56:40Z annales:ART

Enhancement of Yield, Starch, and Amylose Content of Two Indonesian Cassava Genotypes by Producing Gamma Irradiated-Induced Mutants Sudarmonowati, Enny Hartati, N. Sri Supatmi, Supatmi Indonesia cassava genotypes gamma irradiation yield starch amylose content As an important carbohydrate-source food crop and raw materials of starch-based industries,  accelerated cassava breeding has been extensively pursued through various procedures, among others, is mutant induction using gamma irradiation combined with molecular marker. Iding, a local genotype cassava has been compared with a national variety Adira 4 for its yield attributes following gamma irradiation at various dose levels ( 2, 20, 30, and 50 krad). The mutation rate was conducted based on morphological characteristics and identification using RAPD by employing random primer OPB-10. Evaluation was conducted on mutant lines producing high potential yield,  high starch and amylose contents. The fourth generation of irradiated Adira 4 (2 and 30 krad) and Iding (2 krad) exhibited higher yield and amylose content as compared to their representative controls. The yield of 30 krad irradiated Adira 4 indicated by mean tuber weight per individual could reach more than twice of that of control and almost 1.5 times of that irradiated with 2 krad. The levels of amylose content of all irradiated ones, regardless of genotypes and gamma irradiation doses  (2 and 30 krad) were higher than that of control. In most parameters, irradiated Adira 4 was superior to irradiated Iding. National Research and Innovation Agency (BRIN) 2021-01-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4286 10.14203/ann.bogor.2020.v24.n2.95-105 Annales Bogorienses; Vol. 24 No. 2 (2020): Annales Bogorienses; 95-105 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4286/3100 Copyright (c) 2021 Enny Sudarmonowati, N. Sri Hartati, Supatmi Supatmi https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4287 2026-10-19T03:56:40Z annales:ART

Catechin Contents, Antioxidant, and Antibacterial Activities of Different Types of Indonesian Tea (Camellia sinensis) Nuryana, Isa Ratnakomala, Shanti Fahrurrozi, Fahrurrozi Juanssilfero, Ario Betha Andriani, Ade Putra, Filemon Jalu Nusantara Rezamela, Erdiansyah Wulansari, Restu Prawira-Atmaja, M. Iqbal Lisdiyanti, Puspita Antibacterial Camellia sinensis Catechin EGCG Tea Tea is one of the most popular beverages in the world. Produced from Camellia sinensis leaves, tea has been studied to provide health benefits due to the content of important metabolites. This study aimed to investigate the catechin contents, antioxidant, and antibacterial activities of Indonesian tea varieties, namely green tea, black tea, and white tea. Tea infusion was prepared by extracting 1 g of each sample into 10 mL of distilled water and incubated at 80 °C for 60 min. The catechin and epigallocatechin gallate (EGCG) contents of tea extracts were determined using high-performance liquid chromatography (HPLC). Antioxidant activity was measured using the free radical method with 2,2-diphenyl-1-pycrylhidrazyl (DPPH), while antimicrobial activity was assesed using paper disc diffusion assay. The results indicated that green tea had the highest contents of catechin (646 ± 17.14 mg/L) and EGCG (997.8 ± 36.72 mg/L), and antioxidant activity with IC50 of  5.65 µg/mL. Furthermore, green tea and white tea extracts showed inhibitory activity against Gram-positive bacteria such as Micrococcus luteus, Bacillus subtilis, and Staphylococcus aureus whereas black tea had no activity against all bacterial strains tested. Generally, we concluded that white tea and green tea contributed to the higher content of catechins and exhibited strong antioxidant and antibacterial properties. National Research and Innovation Agency (BRIN) 2021-01-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4287 10.14203/ann.bogor.2020.v24.n2.106-113 Annales Bogorienses; Vol. 24 No. 2 (2020): Annales Bogorienses; 106-113 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4287/3101 Copyright (c) 2021 Isa Nuryana, Shanti Ratnakomala, Fahrurrozi Fahrurrozi, Ario Betha Juanssilfero, Ade Andriani, Filemon Jalu Nusantara Putra, Erdiansyah Rezamela, Restu Wulansari, M. Iqbal Prawira-Atmaja, Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4296 2026-10-19T03:56:40Z annales:Pre

Front Pages Volume 25 No 2 (2021) Rahman, Andri National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/4296 Annales Bogorienses; Vol. 25 No. 2 (2021): Annales Bogorienses; i-iii 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4296/3105 Copyright (c) 2026 Andri Rahman https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4297 2026-10-19T03:56:41Z annales:REV

Mini Review: Prostate Cancer Diagnosis and Therapy Fathurahman, Alfi Taufik Swasthikawati, Sri Irawan, Herman Supriatna, Dadang Wardiana, Andri prostate cancer prostate-specific antigen (PSA) docetaxel androgen deprivation therapy (ADT) theranostics Cancer, a non-communicable illness, is the leading cause of death worldwide. In 2030, cancer is expected to exceed 21 million cases and 13 million cancer deaths globally. One of the most prevalent and significant types is prostate cancer (PCa). It is commonly associated with adenocarcinoma, which develops from the mucous glands within the organ. This review highlights available detection systems, and therapeutic options in PCa management. One prevention of deadly PCa is early diagnoses, such as prostate-specific antigen (PSA) screening or genomic profiling. Further testing like MRI or CT scan may also be needed to detect cancers that have progressed to other body regions. There are several possible treatments for PCa, including watchful cancer waiting, surgery, radiotherapy, hormone therapy, and chemotherapy. Based on current studies, androgen deprivation therapy (ADT) combined with docetaxel therapy enhanced great results to treat advanced PCa. The latest development, called theranostics, is a single entity that can perform both diagnostic and therapeutic functions. It can detect disease borders, track therapy in real-time, and provide prognostic data. The FDA has already authorized two prostate-specific membrane antigen (PSMA) positron emission tomography (PET) devices: including Gallium 68 PSMA-11 (Ga 68 PSMA-11) and Pylarify (piflufolastat F 18). National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/4297 10.14203/ann.bogor.2021.v25.n2.55-65 Annales Bogorienses; Vol. 25 No. 2 (2021): Annales Bogorienses; 55-65 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4297/5634 Copyright (c) 2026 Alfi Taufik Fathurahman, Sri Swasthikawati, Herman Irawan, Dadang Supriatna, Andri Wardiana https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4299 2026-10-19T03:56:41Z annales:ART

Factors Affecting Expression Level of Recombinant Human Epidermal Growth Factor in Escherichia coli BL21(DE3) and Size Exclusion Purification Thereof Pratiwi, Riyona Desvy Agustina, Nabella Aminah, Aminah Fuad, Asrul Muhamad IPTG concentration incubation time incubation temperature recombinant human epidermal growth factor Escherichia coli BL21(DE3) Recombinant human epidermal growth factor (rhEGF) has been developed to provide the protein for therapeutic uses. Isopropyl β-d-1-thiogalactopyranoside (IPTG)-induced expression in Escherichia coli BL21(DE3) has shown the most effective system among other inducers systems in a similar host. However, suitable conditions related to IPTG concentration, incubation time, and incubation temperature are different depending on the amino acid content of the recombinant protein. This study aimed to evaluate the effects of various IPTG concentration, incubation time, and incubation temperature on rhEGF concentration. According to each analysis of those factors, induction with 0.05 mM IPTG for 2 h at 23°C was the most appropriate condition to obtain the highest concentration of rhEGF. The rhEGF was positively confirmed with a monoclonal antihEGF antibody and purified in high purity reaching 95.2%, yet recovery was low (1.44%) due to loss in fractions containing endogenous proteins. Therefore, further studies related to type of matrix, column length, and sample concentration in applying size exclusion chromatography are requested for higher recovery National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4299 10.14203/ann.bogor.2021.v25.n2.66-72 Annales Bogorienses; Vol. 25 No. 2 (2021): Annales Bogorienses; 66-72 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4299/3106 Copyright (c) 2026 Riyona Desvy Pratiwi, Nabella Agustina, Aminah Aminah, Asrul Muhamad Fuad https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4300 2026-10-19T03:56:41Z annales:ART

Screening of Active Compounds and LC50 Toxicity Assay of Sunda Porcupine’s (Hystrix javanica F. Cuvier 1823) Quills Crude Extract Budiman, Muhamad Arif Ferdian, Pamungkas Rizki Handayani, Tri Hadi Nugroho, Herjuno Ari Elfirta, Rizki Rabeca Farida, Wartika Rosa Hystrix javanica quills crude extract active compound toxicity Sunda porcupine (Hystrix javanica F. Cuvier 1823) is an endemic fauna from Indonesia that its quills are believed to have medical benefits by local people in some regions of Indonesia. However, the benefits have never been well recorded nor proven scientifically. Local people believe the Sunda porcupine’s quills have efficacy to relieve property for treating toothache. There is limited research on Sunda porcupine’s quills, especially the active compounds, which may affect toothache. This research aims to perform basic pharmacological experiments on Sunda porcupine’s quill samples, which includes screening for the active compounds and determining the LC50 toxicity using brine shrimp lethality test (BSLT) method. Sunda porcupine’s quills were first prepared into simplicia powder (60 mesh in size) and then extracted with 70% ethanol by maceration to produce crude extract. We found that the crude extract of Sunda porcupine’s quills contains some active compounds, including alkaloids, flavonoids, saponins, triterpenoids, steroids, and peptides. The LC50 value of the crude extract was 2,683.19 ppm; thus, categorized as non-toxic. These findings can be used to identify the active compounds in Sunda porcupine’s quills and can be used as a background for further research. National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4300 10.14203/ann.bogor.2021.v25.n2.73-81 Annales Bogorienses; Vol. 25 No. 2 (2021): Annales Bogorienses; 73-81 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4300/3107 Copyright (c) 2026 Muhamad Arif Budiman, Pamungkas Rizki Ferdian, Tri Hadi Handayani, Herjuno Ari Nugroho, Rizki Rabeca Elfirta, Wartika Rosa Farida https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4313 2026-10-19T03:56:41Z annales:ART

Furfural and 5-(Hydroxymethyl) furfural Tolerance Candida strains in Bioethanol Fermentation Ahmad Thontowi Urip Perwitasari Lutfi Nia Kholida Atit Kanti Yopi Bambang Prasetya Furfural 5-Hydroxymethylfurfural Candida bioethanol The toxic fermentation inhibitors in lignocellulosic hydrolysates pose significant problems for the production of second-generation biofuels and biochemicals. Among these inhibitors, 5-(hydroxymethyl)furfural (HMF) and furfural are specifically well known. This study investigated the furfural and 5-HMF tolerance in Candida strains, which could be used for the development of advanced generation bioethanol processes. The 10 isolates of Candida were selected based on the cell growth and bioethanol production on YPD medium containing several concentrations of furfural and 5-HMF by using spectrophotometer and HPLC. Candida parapsilosis Y80 could grow and produce bioethanol in the medium that contains furfural and 5-HMF with a concentration of 60 mM and 40 mM, respectively. Based on the results, C. parapsilosis Y80 has potential activity in the development of bioethanol fermentation. National Research and Innovation Agency (BRIN) 2020-08-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4313 10.14203/ann.bogor.2020.v24.n1.1-10 Annales Bogorienses; Vol. 24 No. 1 (2020): Annales Bogorienses; 1-10 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4313/3119 Copyright (c) 2020 Ahmad Thontowi, Urip Perwitasari, Lutfi Nia Kholida, Atit Kanti, Yopi, Bambang Prasetya https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4314 2026-10-19T03:56:41Z annales:ART

Evaluation of Alternative Components in Growth Media of Lactobacillus brevis for Halal Probiotic Preparation Pratiwi, Riyona Desvy Zanjabila, Sabighoh Fairuza, Dian Aminah, Aminah Praharyawan, Swastika Fuad, Asrul Muhamad halal growth medium Lactobacillus brevis S-layer protein Probiotic has been widely used in functional food because of numerous advantages for health. MRS broth is commonly used as standard medium in studying lactobacilli. However, in some communities - like muslim and vegetarian society, components in MRS broth/medium become an issue. Beef extract and peptone – animal derived substances as nitrogen sources in the MRS medium should be avoided for the vegetarian. Meanwhile, for the muslim society, all components must be halal-certified including those animal derived ingredients. Therefore, several alternative sources for beef extract and peptone substitution were studied. Combination of alternative nitrogen sources was applied. In order to increase the effect of the alternative nitrogen sources, alternative carbon sources were also included. This is the first report about effects of L. brevis media components on cells growth to expression level of surface layer protein (Slp). Whey, lactose, sucrose, and galactose showed high contribution to L. brevis growth. However, the tested concentration of those substances were not sufficient to obtain equal bacterial growth and Slp expression than that of MRS broth. In addition, yeast extract appeared necessary to maintain cell wall and Slp expression. National Research and Innovation Agency (BRIN) 2020-08-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4314 10.14203/ann.bogor.2020.v24.n1.11-17 Annales Bogorienses; Vol. 24 No. 1 (2020): Annales Bogorienses; 11-17 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4314/3120 Copyright (c) 2020 Riyona Desvy Pratiwi, Sabighoh Zanjabila, Dian Fairuza, Aminah Aminah, Swastika Praharyawan, Asrul Muhamad Fuad https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4315 2026-10-19T03:56:41Z annales:ART

Overexpression of Sugarcane Sucrose Transporter 1 (SoSUT1) Gene Increases Rice Yield Putri, Cesha Ananda Soegiharto, Bambang Dewanti, Parawita Rice Sucrose Sucrose Transporter Transgenic Plant The growth and development of plants are determined by photosynthesis, which ultimately results in sucrose. Sucrose is synthesized in the source then translocated to all parts of the plant (sink). The translocation process of sucrose from source to sink is controlled by sucrose protein called sucrose transporter. SoSUT1 is a gene that encodes a sucrose transporter 1 (SUT1) protein in sugarcane. Rice transformation with the SoSUT1 causes overexpression SUT, which is expected to increase the translocation of sucrose into the seed of rice plants. This research was conducted by introducing SoSUT1 in rice plants Inpari 14 SS. Transformation using Agrobacterium tumefaciens vector in apical bud explant Indica rice cv. Inpari 14 SS results in 26 events positive rice contains genes SoSUT1. This study aims to elucidate the inheritance of transgene in the next generation and to characterize its effect on the morphology and the yields of a subsequent generation. The study is conducted by planting the seeds of T1 and T2 plants in media containing Hygromycin and using PCR analysis for further analysis. As the results, from 26 events on the T1 plant, only 3 events of T3 plants were confirmed on the T3 plant. The overexpression of the SoSUT1 gene could increase the number of tillers, the number of productive tillers, panicle length, and panicle exit length, also increasing the number of spherical grains, reducing the number of empty grains and increasing the weight of 1000 grains. National Research and Innovation Agency (BRIN) 2020-08-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4315 10.14203/ann.bogor.2020.v24.n1.18-26 Annales Bogorienses; Vol. 24 No. 1 (2020): Annales Bogorienses; 18-26 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4315/3121 Copyright (c) 2020 Cesha Ananda Putri, Bambang Soegiharto, Parawita Dewanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4391 2026-10-19T03:56:41Z annales:ART

Antidiabetic, Antioxidants and Antibacterial Activities of Lactic Acid Bacteria (LAB) from Masin (Fermented Sauce from Sumbawa, West Nusa Tenggara, Indonesia) Manguntungi, Baso Vanggy, Leggina Rezzy Fidien, Khadijah Alliya Fidien Saputri, Dinar Suksmayu Saputri Mustopa, Apon Zaenal Ekawati, Nurlaili Nurfatwa, Maritsa Prastyowati, Anika Irawan, Shasmita Antidiabetic Antioxidant Antibacterial Lactic Acid Bacteria (LAB) Masin The study aimed to determine the effectiveness of metabolites from Lactic Acid Bacteria (LAB) derived Masin (fermented sauce from Sumbawa) as antioxidant, antidiabetic, and antibacterial compounds. The LAB isolates were isolated from various strains of Staphylococcus piscifermentan which consisted of Staphylococcus piscifermentans strain CIP103958 (code: 2), strain BULST54 (code: 17), strain SK03 (code: 11), strain ATCC 51136 (code: 34), strain PCM 2409 (code: 28) and strain PU-87 (code: 5). The Metabolites of LAB were analyzed by the bioprospecting test to indicate antidiabetic, antioxidant and antibacterial activities. The isolate (Code: 5) at 500 ug/ml showed the most effective antioxidant activity up to 71%. The isolate (code: 28), at  300 ug/ml revealed to have the most antidiabetic activity up to  43 %. The isolate (code: 2) showed moderate antibacterial activity with  the inhibition zone of 5.59 mm. The results of the antidiabetic, antioxidant and antibacterial activity showed that the  secondary metabolites produced by LAB from the Masin have broad activities as an  antidiabetic, antioxidant and antibacterial. National Research and Innovation Agency (BRIN) 2020-08-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4391 10.14203/ann.bogor.2020.v24.n1.27-34 Annales Bogorienses; Vol. 24 No. 1 (2020): Annales Bogorienses; 27-34 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4391/3190 Copyright (c) 2020 Baso Manguntungi, Leggina Rezzy Vanggy, Khadijah Alliya Fidien, Dinar Suksmayu Saputri, Apon Zaenal Mustopa, Nurlaili Ekawati, Maritsa Nurfatwa, Anika Prastyowati, Shasmita Irawan https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4392 2026-10-19T03:56:41Z annales:ART

Assessment of Bacterial Leaf Blight Disease of Indonesian Rice Germplasms Using SSR Markers Fatimah, Fatimah Tasliah, Tasliah Prasetiyono, Joko Mahrup, Mahrup Bacterial leaf blight Molecular marker SSR Xanthomonas oryzae Xa7 Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is a serious disease in rice plants worldwide. Yield losses caused by Xoo can be as high as 50% in some parts of Asia. Xa7 gene can potentially confer a broad resistance to BLB. Evaluation of disease resistance characteristics in early breeding generations of rice is important to develop varieties with better resistance. This study reports the evaluation of 167 Indonesian rice germplasms against three BLB isolates/pathotypes in a green house setting and the genotyping of 56 Indonesian rice germplasm using 12 SSR markers linked to Xa7 BLB resistance gene. The majority of the indigenous rice germplasms was found to be susceptible to three BLB isolates/pathotypes tested. Sate Liko from Bantul, Yogyakarta, Horeg from Cirebon, West Java and Sijem from Malang, East Java revealed consistent resistance to three isolates/pathotypes tested based on BLB evaluation in a greenhouse, UPGMA analysis, and genotyping. Pathotype XII displayed more virulence to Indonesian rice varieties tested compared to pathotypes VI and V. The association analysis using the General linear model identified six markers associated with BLB resistance and two markers were highly associated (RM20589 and RM20590). This information will be useful for future studies of BLB resistance in rice plants. National Research and Innovation Agency (BRIN) 2020-08-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4392 10.14203/ann.bogor.2020.v24.n1.35-49 Annales Bogorienses; Vol. 24 No. 1 (2020): Annales Bogorienses; 35-49 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4392/3191 Copyright (c) 2020 Fatimah Fatimah, Tasliah Tasliah, Joko Prasetiyono, Mahrup Mahrup https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4393 2026-10-19T03:56:41Z annales:ART

In Vitro Ruminal Fermentation and Degradability of Rice Husk on Rice Bran Substitution Fidriyanto, Rusli Ridwan, Roni Astuti, Wulansih Dwi Rohmatussolihat, Rohmatussolihat Nurul Fitri Sar Watman, Muh Widyastuti, Yantyati rice bran rice husk digestibility in vitro Rice bran is a by-product of the rice milling process and has been well used as livestock feed. Rice bran is often adulterated with rice husk. The objective of this study was to evaluate the in vitro ruminal fermentation characteristics of rice bran with various compositions of rice husk and assess the relationship between rice husk addition and rice bran quality. The experiment was arranged in a completely randomized design with rice husk addition as a factor and three replications. Data of proximate value, gas production, ruminal degradability, and volatile fatty acid production were analyzed by analysis of variance. Moreover, significant effects of each treatment in the in vitro fermentation were further analyzed by Duncan's multiple range test (P<0.05). It was shown that the addition of rice husk to rice bran could increase acetic acid level, but it reduced potential gas production, gas production rate, organic matter and dry matter digestibility, and propionic acid level. Interestingly, the linear regression of dry matter digestibility, organic matter digestibility, and potential gas production showed the high adjusted R2 values. Moreover, this study also revealed that 10% of rice husk substitution on rice bran could significantly reduce the dry matter digestibility. National Research and Innovation Agency (BRIN) 2020-08-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4393 10.14203/ann.bogor.2020.v24.n1.50-58 Annales Bogorienses; Vol. 24 No. 1 (2020): Annales Bogorienses; 50-58 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4393/3192 Copyright (c) 2020 Rusli Fidriyanto, Roni Ridwan, Wulansih Dwi Astuti, Rohmatussolihat Rohmatussolihat, Nurul Fitri Sar, Muh Watman, Yantyati Widyastuti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4490 2026-10-19T03:56:41Z annales:ART

Potency of Curcuma aeruginosa as A Chemopreventive Candidate Agent on 7,12-Dimethylbenz[a]anthracene (DMBA)-Induced Rat Spleen Sulfianti, Asri Hasanah, Nur Wibowo, Agung Eru Agustini, Kurnia Artika, I Made chemoprevention cancer Curcuma aeruginosa DMBA wistar spleen Present investigation shows that the extract of C. aeruginosa attenuates DMBA-induced spleen carcinogenesis in Wistar rats. Three-week female Wistar rats were treated with three different C. aeruginosa extract doses (CA1: 40 mg/200 g body weight, BW; CA2: 80 mg/200 g BW; CA3: 160 mg/200 g BW) and were induced with DMBA after one-week administration of these doses. A commercial immunostimulant, and DMBA only were also given to each group as positive and negative control, respectively. The development of tumors was evaluated by investigating the incidence of tumor and tumor multiplicity during the experiment. Spleen mass index and histological parameters such as white pulp, centrum germinativum, and marginalis zone were also examined. Based on our study, the administration of C. aeruginosa extract during and after carcinogen induction gave several impacts on rat carcinogenesis. At the extract dose of 80 mg/200 g BW, tumor incidence of animals were least (P<0.05). However, all doses did not show any effect to the spleen mass index, though the highest dose (160 mg/200 g BW) was found to cause changes in white pulp and marginalis zone boards. This trend indicates that it takes higher dose to cause an immune response effect reaching the organs. National Research and Innovation Agency (BRIN) 2020-05-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4490 10.14203/ann.bogor.2019.v23.n2.58-65 Annales Bogorienses; Vol. 23 No. 2 (2019): Annales Bogorienses; 58-65 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4490/3275 Copyright (c) 2020 Asri Sulfianti, Nur Hasanah, Agung Eru Wibowo, Kurnia Agustini, I Made Artika https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4496 2026-10-19T03:56:41Z annales:ART

Laccase and Manganese Peroxidase (MnP) Activities in the White-Rot Fungus Trametes hirsuta in Response to Aromatic Compounds Nuryana, Isa Ilmiah, Zidny Andriani, Ade Yopi, Yopi Aromatic compounds Glucose Yeast Peptone medium Laccase Manganese peroxidase Trametes hirsuta The current interest in exploring white-rot fungi has been concentrated in increasing their ligninolityc enzyme production such as laccase and manganese peroxidase (MnP) due to the great value in industrial application. The presence of appropiate inducers can enhance the enzyme production. Hence, the aim of the study was to investigate the activity of  laccase and MnP in response to various aromatic compounds. The fungus Trametes hirsuta was cultured in Glucose Yeast Peptone (GYP) broth (pH 5.0) with the addition of 0.5% of sorghum biomass as substrate. The cultures were then incubated on rotary shaker at 150 rpm at 27oC. The 7-day-old cultures were then supplemented with different aromatic compounds, namely caffeic acid, gallic acid, syringic acid, trans-cinnamic acid, vanillin, and veratryl alcohol. Our results demonstrated that veratryl alcohol, gallic acid and vanillin gave an inductive effect on the value of laccase and MnP activities, with vanillin showing the highest induction. On the contrary, the level of laccase and MnP activities remained low in the presence of aromatic compound such as syringic acid, trans-cinnamic acid and caffeic acid. To conclude, our study reveals that aromatic compounds can be potential as inducers and may contribute to the improvement of laccase and MnP productivity by T. hirsuta. National Research and Innovation Agency (BRIN) 2020-05-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4496 10.14203/ann.bogor.2019.v23.n2.66-71 Annales Bogorienses; Vol. 23 No. 2 (2019): Annales Bogorienses; 66-71 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4496/3283 Copyright (c) 2020 Isa Nuryana, Zidny Ilmiah, Ade Andriani, Yopi Yopi https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4500 2026-10-19T03:56:41Z annales:ART

Development of Mice Embryo (Mus musculus L.) after Closed Pulled Straw Vitrification in CZB Medium Gunawan, Muhammad Kaiin, Ekayanti Mulyawati Rusherdiannita, Raden Cindy Alipin, Kartiawati Mice Blastocyst Close Pulled Straw (CPS) Cryoprotectants CZB Medium The objective of our present experiment was to investigate the effects of closed pulled straw (CPS) vitrification on the viability and development of mouse embryo. The experiment was arranged according to completely randomized design (CRD) consisting of 4 treatments, namely not vitrification (NV) is control, CPS 1 (mCZB Hepes + 20% Bovine Serum Albumin (BSA) +0.5 M sucrose + 10% EG + 10% DMSO), CPS 2 (mCZB Hepes + 20% BSA + 0.5 M sucrose + 15% EG + 15% DMSO), and CPS 3 (mCZB Hepes + 20% BSA +0.5 M sucrose + 20% EG + 20% DMSO) with 6 replications. The viability of embryos (%) was determined after 24 - 72 h of the culture period, while we also observed the percentage of embryos reaching the blastocyst stage (early blastocyst, expanded, hatching, and hatched). As a result, the treatments did not give a significant difference in the viability of embryos (P<0,05) but showed significant effects on embryo development (P<0,05). Furthermore, this present work conclusively found that CPS vitrification in CZB medium with cryoprotectants ethylene glycol (EG) and dimethyl sulfoxide (DMSO) noticeably influenced the development of mice embryo to reach the blastocyst stage, but showed no remarkable difference in the viability of embryo after culture for 24 – 72 h. National Research and Innovation Agency (BRIN) 2020-05-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4500 10.14203/ann.bogor.2019.v23.n2.72-80 Annales Bogorienses; Vol. 23 No. 2 (2019): Annales Bogorienses; 72-80 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4500/3286 Copyright (c) 2020 Muhammad Gunawan, Ekayanti Mulyawati Kaiin, Raden Cindy Rusherdiannita, Kartiawati Alipin https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4502 2026-10-19T03:56:41Z annales:ART

Cytotoxic Activity of Flavonoid Compounds Isolated from Tetrameles Nudliflora Leaves Megawati, Megawati Darmawan, Akhmad Fajriah, Sofa Tetrameles nudiflora flavonoid kaempferol quercetin breast carcinoma MCF-7 cancer cell lines Tetrameles nudliflora is one of the endemic plants in Wallace region, especially in Mekongga Forest, Southeast Sulawesi, Indonesia. As part of our project in the field of natural drug discovery based on local bioresources, this study aimed to isolate secondary metabolite compounds from the ethyl acetate fractions of the leaves of T. nudiflora and evaluate their cytotoxic activities against breast carcinoma cancer lines, MCF-7. The methods used were various chromatography techniques such as vacuum column chromatography, gravitation column chromatography, thin layer chromatography, preparative thin layer chromatography, and re-crystallization using two different organic solvents. In the present study, two flavonoid compounds, kaempferol (1) and quercetin (2) were identified. The structures of 1-2 were determined using serial spectroscopic techniques, including mass spectrometry, one- and two-dimensional nuclear magnetic resonance. Moreover, using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay method, both compounds showed in vitro cytotoxic activity IC50 of 34.162 and 133.148 μg mL-1, respectively against MCF-7 cell lines. National Research and Innovation Agency (BRIN) 2020-05-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4502 10.14203/ann.bogor.2019.v23.n2.81-86 Annales Bogorienses; Vol. 23 No. 2 (2019): Annales Bogorienses; 81-86 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4502/3287 Copyright (c) 2020 Megawati Megawati, Akhmad Darmawan, Sofa Fajriah https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4504 2026-10-19T03:56:41Z annales:ART

Field Evaluation of Conde Progenies with Early Heading Date and Resistance to Bacterial Leaf Blight Fatimah, Fatimah Prasetiyono, Joko Dadang, Ahmad Tasliah, Tasliah Masumah, Masumah Hd2 Maros Sukamandi Tropical region Xa7 Heading date is one of key traits in rice adaptation to different environments. Rice with early maturity is very useful for increasing rice production extensively especially in Indonesia. The improvement of rice variety Conde for heading date and resistance to bacterial leaf blight (BLB) was developed through Marker Assisted Backcrossing approach (MAB). Xa7 derived from Conde as recipient parents for resistance to BLB and Hd2 gene derived from Nipponbare as a donor parent for heading date. The aim of this research was to evaluate the performance of heading date and agronomic traits of 85 BC2F4 lines in two field stations, Sukamandi (West Java) and Maros (South Sulawesi). Molecular analysis of Xa7 on chromosome 6 and Hd2 gene on chromosome 7 and agronomic performance was evaluated. Foreground analysis resulted 41% for Xa7 and 66.7% for Hd2 gene in homozygous condition. The majority of lines have similar heading date and yield with Conde and more resistance to bacterial leaf blight (BLB) than Conde. Six lines flowered earlier 5 days than Conde in Sukamandi and three lines flowered earlier 3 days in Maros. Twelve lines with flowered earlier than Conde, resistance to BLB and similar yield with Conde were selected for background analysis resulted 93% genome recovery. These lines would be further evaluated in preliminary yield trail for their stability. National Research and Innovation Agency (BRIN) 2020-05-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4504 10.14203/ann.bogor.2019.v23.n2.87-96 Annales Bogorienses; Vol. 23 No. 2 (2019): Annales Bogorienses; 87-96 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4504/3288 Copyright (c) 2020 Fatimah Fatimah, Joko Prasetiyono, Ahmad Dadang, Tasliah Tasliah, Masumah Masumah https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4505 2026-10-19T03:56:41Z annales:ART

Diversity and Antimicrobial Activity of Lichens-Associated Actinomycetes in Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) Indonesia Susanti, Agustina Eko Ratnakomala, Shanti Mangunwardoyo, Wibowo Lisdiyanti, Puspita Actinomycetes Antimicrobial Diversity Identification 16srRNA Lichens     Bioprospecting has developed to all biological taxa including procaryotic. Actinomycetes become interesting procaryotic because of the ability to produce important secondary metabolite for human life. Actinomycetes are known as the largest antibiotic producer that has a broad range habitat. Some research has been done to find new antibiotic from the various habitat of actinomycetes. One of the interesting habitats of actinomycetes which never been explored in Indonesia is lichens... Lichens as the symbiotic structure of alga and fungi areknown as the ecological niche of various kinds of microorganisms including actinomycetes. Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) have various species of trees as the habitat of lichens. These areas are known as one of the research locations to explore the biodiversity of Indonesia. The aims of this research is to study the diversity and antimicrobial potency of actinomycetes isolated from 10 lichen samples with various type of thallus; crustose, fructose and foliose. Lichen samples were grown on the bark of 9 trees species in CSC and CBG. Isolation process used three agar media; HV, YIM6 and YIM711 with cycloheximide and nalidixic acid. Molecular identification based on 16S rRNA gene sequence. Antimicrobial activity was tested to 65 isolates by agar diffusion method to Bacillus subtilis BTCC B.612, Escherichia coli BTCC B.614, Candida albicans BTCC Y.33, Staphylococcus aureus BTCC B.611, Micrococcus luteus BTCC B.552. Isolation process retrieved 125 isolates with the highest number grow on HV agar medium. Based on the sample, the highest number of actinomycetes were isolated from crustose lichen attached on the bark of Averrhoea carambola. A total 69 isolates were identified as the genera Actinoplanes, Amycolatopsis, Angustibacter, Kribbella, Micromonospora, Mycobacterium, and Streptomyces. The screening process showed 24 isolates have antimicrobial activity, with the highest inhibitory activity against Micrococcus luteus BTCC B.552. National Research and Innovation Agency (BRIN) 2019-10-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4505 10.14203/ann.bogor.2019.v23.n1.1-12 Annales Bogorienses; Vol. 23 No. 1 (2019): Annales Bogorienses; 1-12 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4505/3289 Copyright (c) 2019 Agustina Eko Susanti, Shanti Ratnakomala, Wibowo Mangunwardoyo, Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4506 2026-10-19T03:56:41Z annales:ART

Morphology and Molecular Characterization of Newly Isolated Microalgae Strain Chlorella volutis LIPI13-WKT066 from Wakatobi Islands and Its Potential Use Rahman, Delicia Yunita Praharyawan, Swastika Fadiyah, Farizul Raharjo, Sapto Susilaningsih, Dwi Chlorella volutis Wakatobi islands Marine environment Health supplement Morphology and molecular characterization of microalgae isolated from Wakatobi Marine National Park was conducted. An understanding of the characteristics of morphology, molecular, as well as metabolites profile of the microalgae species is potentially useful for its future application. The primary aim of this study was to isolate, identify and characterize the microalgae strain isolated from Wakatobi Marine National Park labeled as LIPI13-WKT066 with the emphasis on the evaluation of amino acid content as a basis for its health application. Morphological observation under the microscope and molecular identification suggested that the microalgae strain of LIPI13-WKT066 belong to the strain under species of Chlorella volutis. Metabolite characterization of the microalgae strain showed that the content of protein (11.9%), lipid (12.4%) and carbohydrate (4.7%) was in the regular range. Further analysis of its amino acid content revealed the potency of the microalgae strain to be used as antihypertensive agent. National Research and Innovation Agency (BRIN) 2019-10-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4506 10.14203/ann.bogor.2019.v23.n1.13-19 Annales Bogorienses; Vol. 23 No. 1 (2019): Annales Bogorienses; 13-19 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4506/3295 Copyright (c) 2019 Delicia Yunita Rahman, Swastika Praharyawan, Farizul Fadiyah, Sapto Raharjo, Dwi Susilaningsih https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4513 2026-10-19T03:56:41Z annales:ART

Enzyme Production from Cassava Peels by Aspergillus Awamori KT-11: The Making of Natural Sweetener from Several Tubbers Melliawati, Ruth Rahman, Farida Aspergillus awamori KT-11 amylase tuber sugar The use of cassava (Manihot esculenta Crantz) peel for enzyme production has not been widely used. The purpose of this study was to produce complex amylase enzymes from cassava peel by A. awamori KT-11 and apply them in the manufacture of natural sweeteners. Enzyme production is carried out on red and white cassava peel. Media of cassava peel sterilized, inoculated with 1%  A. awamori KT-11, incubated for 5 days, then dried at 50°C and mashed. Making sugar is done on cassava flour, sweet potato ( Ipomoea batatas L), taro (Colocasia esculenta) and cocoyam (Xanthosoma sagittifolium) with different concentrations of 10%, 15%, 20%, and 15% and 20% enzyme concentrations. The hydrolysis process is carried out for 3 days at 60°C. The enzyme activity in red cassava peel was 405,006 U/mL and white cassava peel was 321,239 U/ml. The sugar produced in cassava, taro, sweet potato, and Cocoyam was 101.38 mg/mL, 81.18 mg/mL, 55.929 mg/mL, and 42.874 mg/mL, respectively. The results of TLC showed that cassava and taro sugar  contain maltose, lactose and glucose, sweet potatoes contained glucose and dextrin and Cocoyam containing fructose. The sweetness level of sugar from cassava, taro, sweet potato and Cocoyam is 14 brix, 12 brix, 9 brix and 9 brix, respectively. National Research and Innovation Agency (BRIN) 2019-10-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4513 10.14203/ann.bogor.2019.v23.n1.20-29 Annales Bogorienses; Vol. 23 No. 1 (2019): Annales Bogorienses; 20-29 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4513/3298 Copyright (c) 2019 Ruth Melliawati, Farida Rahman https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4516 2026-10-19T03:56:41Z annales:ART

Overexpression of OsHox-6 Gene Enhanced Tiller Number in Rice But Induced Yield Penalty Rahmawati, Syamsidah Chairunnisa, Chairunnisa Erdayani, Eva Nugroho, Satya Estiati, Amy rice drought IR64 Sub1 HD-Zip OsHox-6 OsHox-6, belongs to the transcription factor homeodomain leucine zipper (HD-Zip) protein sub-family I, has unknown function. This study was aimed to characterize the phenotypes of two homozygous transgenic rice lines (S29-62-2 and S.40.4-158-1) containing an extra copy of OsHox-6 gene under the control of a rice constitutive promoter, OsLEA3, and to evaluate their tolerance to water stress. A real-time quantitative PCR (qRT-PCR) showed that the transcript expression of OsHox-6 gene in the transgenic lines increased 5-10 folds under a normal irrigation and 10-20 folds after exposure to water stress conditions as compared to its wild type control. Transgenic plants overexpressing OsHox-6 exhibited phenotypic alteration at the normal irrigation by inducing tiller formation, suggesting a decrease in the apical dominance. Transgenic plants also showed significant enhancement in the total grain number, however, the number of empty grains  also increased significantly (~16-22%).  After imposed to the water stress, the number of empty grains in the transgenic lines was even higher (up to 83% in average). Furthermore, observations on the water loss rates, relative water contents and drought resistance indices (DRI) suggested that the overexpression of OsHox-6 did not significantly increase tolerance to water stress.  Further research is required to reveal the detailed mechanisms of OsHox-6 in response to water and other abiotic stresses. National Research and Innovation Agency (BRIN) 2019-10-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4516 10.14203/ann.bogor.2019.v23.n1.30-40 Annales Bogorienses; Vol. 23 No. 1 (2019): Annales Bogorienses; 30-40 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4516/3325 Copyright (c) 2019 Syamsidah Rahmawati, Chairunnisa Chairunnisa, Eva Erdayani, Satya Nugroho, Amy Estiati https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4555 2026-10-19T03:56:41Z annales:ART

Identification of Aquatic Plant Species from Bantimurung Waterfall and Their Phytochemical Compounds Analysis Nugraha, Media Fitri Isma Maharani, Fasya Hadaina Novita, Hessy Rajamuddin, Muh Alias L Yunita, Rossa Reflinur, Reflinur Enggarini, Wening Julzarika, Atriyon Elya, Berna Aquatic plants Antioxidant activity Flavonoid Phenolic A total of 21 aquatic plant species were collected from Bantimurung waterfall areas, South Sulawesi, Indonesia, in October 2017. These plant materials were subjected to both species and phytochemicals identification. The aims of this study were to determine the species or taxonomic rank of Indonesian aquatic plants collected from Bantimurung waterfall, South Sulawesi, Indonesia and to identify their chemical compounds (phytochemicals) as a candidate for new herbal medicine. Plant genetic materials used in this study were collected from Bantimurung Bulusaraung waterfall and were then identified based on standard botanical techniques for species identification in the Herbarium Bogoriense, Research center for Biology Indonesian Institute of Science (LIPI), Cibinong, West Java. The samples were subjected to the phytochemistry screening such as alkaloids, flavonoids, tannins, saponins, glycosides, terpenoids and anthraquinone followed the procedures of Indonesian Materia Medika and Harborne.  Results showed that all collected aquatic plant samples were able to be identified, including their species names. Phytochemical screening of each sample revealed the presence of glycoside in all of the tested species. However, no alkaloids, anthraquinones, and terpenoids were observed in those tested plant samples. Of the total 21 aquatic plants, 14 species contained flavonoids, 8 species contained phenol compound, and 10 species contained saponins. Among these species Donnax canniformis possessed good antioxidant activity, which correlated to its total phenolic and flavonoid contents. Our results would be beneficial for any future effort in the development of new herbal drugs derived from aquatic plants. National Research and Innovation Agency (BRIN) 2019-10-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4555 10.14203/ann.bogor.2019.v23.n1.41-48 Annales Bogorienses; Vol. 23 No. 1 (2019): Annales Bogorienses; 41-48 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4555/3329 Copyright (c) 2019 Media Fitri Isma Nugraha, Fasya Hadaina Maharani, Hessy Novita, Muh Alias L Rajamuddin, Rossa Yunita, Reflinur Reflinur, Wening Enggarini, Atriyon Julzarika, Berna Elya https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4556 2026-10-19T03:56:41Z annales:ART

Understanding the Increased Fruit Content of Soluble Proteins, Sugars, Certain Enzyme Activities, and Certain Polyphenols in Context to Date Fruit Ripening Alqarni, Saad Salman M. Alqarni Bazzi, Mohammad D. Dates Ripening Enzymes Fruit Introduction: To understand the increased fruit content of soluble proteins, sugars, certain enzymes activities, and certain polyphenols in context to date fruit ripening. Materials and Methods: The study collected four varieties of date fruits from a farm in Riyadh, Saudi Arabia. In it, 10-14-day intervals separated the various stages of maturity. The process of sample preparation and extraction includes the samples of fruits at different stages of maturation. Results and Discussion: The Polyphenols oxidize activity appeared to decline with further maturation of date fruit. There was an increase in the protein content and the reducing sugar in all varieties of dates. The changes in the total sugar and reducing sugar occurred due to the increased enzyme’s activity. Conclusion: The reducing sugar increased in all varieties of the date fruit. The coordination of the enzyme might influence the quality of the ripening of date fruits. National Research and Innovation Agency (BRIN) 2019-10-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4556 10.14203/ann.bogor.2019.v23.n1.49-57 Annales Bogorienses; Vol. 23 No. 1 (2019): Annales Bogorienses; 49-57 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4556/3330 Copyright (c) 2019 Saad Salman M. Alqarni Alqarni, Mohammad D. Bazzi https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4557 2026-10-19T03:56:41Z annales:ART

Partial Purification, Characterization, and Application of Extracellular Aspartic Protease from Lactobacillus casei WSP in Producing the Bioactive Peptides with Antibacterial and Antioxidant Activity Solikhin, Akhmad Mustopa, Apon Zaenal Suharsono, Suharsono Putranto, Wendry Setiyadi Aspartic protease Antibacterial Antioxidant Peptide    Lactobacillus casei WSP-derived an aspartic protease was sequentially purified by using chromatography gel filtration sephadex G-50. It resulted in a 22.81-fold increase of specific activity (51.5 U/mg) with a final yield of 1.9%. The estimated molecular weight of the purified enzyme was 37 kDa and showed gelatinolytic activity in zymogram assay. The enzyme exhibited optimum activity at 40ºC and pH 6 with casein as the substrate. Enzyme activity was significantly inhibited by pepstatin A (0.5 mM and 1 mM), confirming that this enzyme is a group of aspartic proteases, while other inhibitors such as EDTA, PMSF and iodoacetic acid showed no inhibition effect on the activity of enzyme. The addition of metal ion to the enzyme decreased enzyme activity, indicating the proteolytic enzyme was metal ion- dependent. Denaturant such as DDT tended to increase caseinolytic activity. Furthermore, this enzyme was capable of generating the new peptides from skimmed milk with the size 8 kDa, 10 kDa and 15 kDa. These peptides have potential as antibacterial and antioxidant agents.   National Research and Innovation Agency (BRIN) 2018-12-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4557 10.14203/ann.bogor.2018.v22.n2.47-56 Annales Bogorienses; Vol. 22 No. 2 (2018): Annales Bogorienses; 47-56 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4557/3331 Copyright (c) 2018 Akhmad Solikhin, Apon Zaenal Mustopa, Suharsono Suharsono, Wendry Setiyadi Putranto https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4558 2026-10-19T03:56:41Z annales:ART

Optimization of Expression Condition, Two Dimensional And Melting Point-Based Characterization of Recombinant Human Interferon Alpha-2a Fusion and Non Fusion Forms Ningrum, Ratih Asmana Wardhani, Widdya Kusuma Wahyuni, Ike Mustopa, Apon Zaenal Recombinant Human Interferon Alpha-2a Pichia pastoris overproduction 2D-SDS PAGE and melting point      Recombinant Human Interferon Alpha-2a (rhIFNα-2a) is a therapeutic protein that used in hepatitis and cancer treatments. In our previous research, we developed higher molecular weight of the protein through human serum albumin fusion. The fusion and non fusion form of rhIFNα-2a were produced in Pichia pastoriswith 86 kDa and 19 kDa in size respectively. In previous research, protein yield was not reproducible due to unoptimized expression conditions. This reseach was aimed to optimize expression condition process and to characterize the fusion and non fusion forms of rhIFNα-2a. The parameters to observe in overproduction include nutrient (media and methanol concentration) and non nutrient (temperature andincubation period). Affinity and size exclusion cromatographicwere compared in protein purification. BCA assay was used to determine quantity of protein. Protein characterization was conducted using two-dimensional SDS PAGE and denaturation analyses. The optimal condition of expression was achieved using complex media with 1% of methanol for 3 day incubation period at 25°C. The protein yield was reproducible and higher comparing to previous research. Affinity chromatography resulted in higher purity of the proteins comparing to size exclusions. Characterization using two dimensional gel analysis revealed that isoelectric point of rhIFNα-2a is 6.5 for fusion form and 6.0 for non fusion form. The melting points of fusion protein were 56°C and 62°C whilst that of non fusion was 56°C. National Research and Innovation Agency (BRIN) 2018-12-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4558 10.14203/ann.bogor.2018.v22.n2.57-64 Annales Bogorienses; Vol. 22 No. 2 (2018): Annales Bogorienses; 57-64 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4558/3332 Copyright (c) 2018 Ratih Asmana Ningrum, Widdya Kusuma Wardhani, Ike Wahyuni, Apon Zaenal Mustopa https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4875 2026-10-19T03:56:42Z annales:ART

DNA Condensation Study of Fully Synthesized Lipopeptide-Based Transfection Agent for Gene Delivery Vehicle Tarwadi, Tarwadi Rachmawati, Heni Kartasasmita, Rahmana E. Pambudi, Sabar Arbianto, Alfan Danny Restiani, Dewi Esti Asyarie, Sukmadjaja lipopeptide transfection agent gene delivery and DNA condensation study    The main requirement of transfection agent has to condense DNA in nanoparticle size, protect the DNA from nucleases and other degrading enzymes during its transport in cell cytoplasm and nucleus and should not toxic to target cells. In this research, lipopeptide composed of palmitoyl (C-16) and short peptide sequence have been designed fully synthesized and tested to DNA condensation capability and toxicity. The DNA condensation study was performed using EtBr exclusion assay and cytotoxicity determination was carried out by colorimetric MTT assay. It was revealed that lipopeptide-based transfection agent of Pal-CKKHH and Pal-CKKHH-YGRKKRRQRRR-PKKKRKV condensed DNA molecules efficiently. The lipopeptide was less toxic compared to Lipofectamine and Poly-L-Lysine, that shown by 90% of CHO-K1 cells remained viable when they were treated with 4.36 µM Pal-CKKHHYGRKKRRQRRR-PKKKRKV. Meanwhile, there were only ~75% and 80% of CHO-K1 viable cells when it was treated with PLL and Lipofectamine®2000, respectively. Moreover, cell viability of HepG2 was ~ 75% after treated with 2.18 µM of Pal-CKKHH-YGRKKRRQRRR-PKKKRKV and decreased to ~65% when the lipopeptide concentration increased to 8.72 M. In summary, the synthesized lipopeptide condenses DNA molecules efficiently, less toxic than Lipofectamine®2000 and PLL and has possibility to be explored as a non-viral gene delivery vehicle. National Research and Innovation Agency (BRIN) 2018-12-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4875 10.14203/ann.bogor.2018.v22.n2.65-74 Annales Bogorienses; Vol. 22 No. 2 (2018): Annales Bogorienses; 65-74 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4875/3615 Copyright (c) 2018 Tarwadi Tarwadi, Heni Rachmawati, Rahmana E. Kartasasmita, Sabar Pambudi, Alfan Danny Arbianto, Dewi Esti Restiani, Sukmadjaja Asyarie https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4876 2026-10-19T03:56:42Z annales:ART

Non Invasive Detection of Dengue Viruses from Saliva: In vitro Study Saksono, Budi nanGlycoscience real time RT-PCR Nanotechnology non-invasive detection       In the previous paper, we had succeeded in developing an early detection system of dengue viruses using Sugar liganded Gold Nano Particle (SGNP) only from 6 μL serum. It has been reported that dengue virus is also detected in the saliva and urine of the patient. The evidences lead to the possibility of developing non-invasive methods of dengue virus detection. In this in vitro study, we evaluated the utility of SGNP to capture and concentrate dengue virion in 10% saliva solution. The results showed that dengue virion was successfully detected in 10% of saliva solution. Analysis of virion stability during storage showed that virions in salivary samples were stable up to 3 days at temperature wherease the RNA has significantly degraded. Although still a preliminary study, the data obtained show the prospect of SGNP as a non-invasive dengue virus detection method, as well as the development of POC (Point of Care) method. Clinical trials using saliva from dengue viruses infected patients need to be done to prove the effectiveness of the SGNP method. National Research and Innovation Agency (BRIN) 2018-12-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4876 10.14203/ann.bogor.2018.v22.n2.75-80 Annales Bogorienses; Vol. 22 No. 2 (2018): Annales Bogorienses; 75-80 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4876/3617 Copyright (c) 2018 Budi Saksono https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4877 2026-10-19T03:56:42Z annales:ART

Amelioration of Salt Tolerance in Soybean (Glycine Max. L) by Plant-Growth Promoting Endophytic Bacteria Produce 1-Aminocyclopropane-1-Carboxylase Deaminase Simarmata, Rumella Ngadiman, Ngadiman Rohman, Saifur Simanjuntak, Partomuan      Salinity is a major abiotic stress that can induce ethylene synthesis beyond the normal limits as plants response to stress and hence reduces crop productivity. The 1-aminocyclopropane-1-carboxylase deaminase (ACCD)-producing bacteria can reduce excessive ethylene synthesis by taking ACC (ethylene precursor) as a nitrogen source. This study showed the possibility of using endophytic bacteria in order to reduce the undesirable effects of salinity. Strain Pseudomonas putida PIR3C and Roultella terrigena PCM8 exhibited promising performance for promoting the growth of plant under salinity stress conditions. The results showed that bacterial inoculation was effective even in the presence of higher salinity levels. Strain P. putida PIR3C was the most efficient strain compared to the other strains and significantly increased shoot length, root length, dry weight, germination percentage, and reduced stem diameter. The role of ACCD in reducing ethylene production under salinity stress conditions was also studied by measuring the evolution of ethylene in vitro by soybean seeds treated with some ACCD bacterial strain. The maximum ethylene lowering capacity was observed in R. terrigena PCM8, the strain reduced ethylene production from 622.81 nmol.g-1(control) to 352.78 nmol.g-1 (43% reduction). The production of α-ketobutyrate, chlorophyll content and germination percentage from P. putida PIR3C was higher than other strains. The results suggested that strain P. putida PIR3C and R. terrigena PCM8 can be employed for salinity tolerance in soybean seedlings and may have better prospects for an amelioration of stress condition.  National Research and Innovation Agency (BRIN) 2018-12-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4877 10.14203/ann.bogor.2018.v22.n2.81-93 Annales Bogorienses; Vol. 22 No. 2 (2018): Annales Bogorienses; 81-93 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4877/3618 Copyright (c) 2018 Rumella Simarmata, Ngadiman Ngadiman, Saifur Rohman, Partomuan Simanjuntak https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4878 2026-10-19T03:56:42Z annales:ART

Molecular Evaluation for Drought Tolerant Using Marker Assisted Breeding Method Fatimah, Fatimah Prasetiyono, Joko Trijatmiko, Kurniawan Rudi Sustiprijatno, Sustiprijatno Cabacu Inpari 30 marker assisted backcrossing pyramid Situ Bagendit    The sustainability and increasing the national rice production require the readiness of food and agriculture sector cope with the impacts of climate change, land degradation, drought area, sloping production and the raising of population growth. Adaptation plays an important role in ensuring the sustainability of food security. This research aimed to develop drought-tolerant variety of Inpari 30 (submergence tolerance variety) and Situ Bagendit through marker-assisted backcrossing-through pyramiding gene of identified QTLs for foreground selection and to explore SSRs and 6K SNPs for background selection distributed in 12 rice chromosome of drought tolerant donor (Cabacu) and recipient rice (Inpari 30 and Situ Bagendit). The foreground selection revealed that flanking SSRs of each QTLs (qRPF2.1, qGPP2.1, qSPP4.1 and Sub1) was less than 2 cM. The background selection through polymorphic survey of Rice 6K SNP primers revealed 2457 (53,3%) polymorphic SNPs on Inpari 30 vs Cabacu and 2563 (55,6%) polymorphic SNPs on Situ Bagendit vs Cabacu with the average distance about 0.74 cM/chromosome. The genotypic selection of F1 Inpari 30/Cabacu and F1 Situ Bagendit/Cabacu have already in heterozygote condition for these 4 QTLs target. These lines was continued for backcross breeding to develop BC1F1 Inpari 30/Cabacu and BC1F1 Situ Bagendit/Cabacu generation. National Research and Innovation Agency (BRIN) 2018-12-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4878 10.14203/ann.bogor.2018.v22.n2.94-100 Annales Bogorienses; Vol. 22 No. 2 (2018): Annales Bogorienses; 94-100 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4878/3619 Copyright (c) 2018 Fatimah Fatimah, Joko Prasetiyono, Kurniawan Rudi Trijatmiko, Sustiprijatno Sustiprijatno https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4879 2026-10-19T03:56:42Z annales:ART

Molecular Docking and Molecular Dynamics Study of Alcoholic Compounds as Mycobactericidal Agents Using InhA, MabA and PanK as Receptors Syahputra, Gita Arwansyah, Arwansyah Kusharyoto, Wien      Tuberculosis (TB) infection is one of the primaryinfectious diseases in many developing countries; even there are minor cases in some developed countries. TB infection spread through the air and ismore probable when using improper disinfectant on medical and laboratory equipment which related to TB research. The appropriate disinfectants which are commonly usedin laboratory equipment can reduce the risk of transmission of TB disease. Alcoholic compoundsare one of the common disinfectants with a broad spectrum activity towardsmicrobes,viruses, and fungus. We employed molecular docking and molecular dynamics simulation to support virtual screening and ligand-receptor complex binding observation in searching for an appropriate mycobactericidal agent.Based on the analysis of molecular docking and molecular dynamics, pentadecanol has potency as a mycobactericidal agent with PanK as itsspecific receptor. The Gibbs free energy (∆G) for the interaction of pentadecanol with PanKhas been found to be -5.5 kcal/mol. Molecular dynamics analysis at 300K and 1 atm for 5 ns showed a little change in the confirmation of the binding site, whilepentadecanolwas still being bound by its binding siteon PanK. National Research and Innovation Agency (BRIN) 2018-12-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4879 10.14203/ann.bogor.2018.v22.n2.101-115 Annales Bogorienses; Vol. 22 No. 2 (2018): Annales Bogorienses; 101-115 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4879/3627 Copyright (c) 2018 Gita Syahputra, Arwansyah Arwansyah, Wien Kusharyoto https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4883 2026-10-19T03:56:42Z annales:ART

Medium Optimization for Antimicrobial Production By Newly Screened Lactic Acid Bacteria Rohmatussolihat, Rohmatussolihat Lisdiyanti, Puspita Yopi, Yopi Widyastuti, Yantyati Sukara, Endang Antimicrobial Lactobacillus plantarum Response Surface Methodology Lactic acid bacteria (LAB) are important for prevention of spoilage and pathogenic bacterial growth in foods due to their ability to generate antimicrobial substances. The objective of this study was to screen LAB for antimicrobial activity and to optimize culture medium for antimicrobial production using Response Surface Methodology (RSM) with Central Composite Design (CCD). Optimization of antimicrobial production of selected LAB was conducted with different combinations of glucose, NaCl, inoculum, and temperature. Our experimental results showed that from 129 LAB isolates, 55 showed significant inhibition against Bacillus subtilis, Escherichia coli, Micrococcus luteus, Staphylococcus aureus, Aspergillus niger, and Candida albicans. No isolates inhibited the growth of Aspergillus flavus. Lactobacillus plantarum LIPI13-2-LAB011 was selected for further study on culture medium optimization to inhibit the growth of C. albicans. From statistical analysis, the production of antimicrobial substances was significantly influenced by temperature, NaCl, and concentration of glucose. Furthermore, the optimum concentrations of glucose, concentration of inoculum, temperature, and NaCl were 1.63 %, 3.03%, 33.74°C, and 3.4%, respectively, with a maximum predicted inhibition index of 1.916, which increased 3.56-fold compared to that obtained in medium before optimization processes. The result was confirmed as when the optimum concentration of nutritions used, the inhibition index increased 3.12-fold. National Research and Innovation Agency (BRIN) 2018-06-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4883 10.14203/ann.bogor.2018.v22.n1.1-11 Annales Bogorienses; Vol. 22 No. 1 (2018): Annales Bogorienses; 1-11 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4883/3634 Copyright (c) 2018 Rohmatussolihat Rohmatussolihat, Puspita Lisdiyanti, Yopi Yopi, Yantyati Widyastuti, Endang Sukara https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4884 2026-10-19T03:56:42Z annales:ART

Medium Chain and Long Chain Alkanes Hydroxylase Producing Whole Cell Biocatalyst From Marine Bacteria Thontowi, Ahmad Yetti, Elvi Yopi, Yopi alkane alkane hydroxylase marine bacteria Alkanes are  major component of crude oil that could be hydrolyzed by the enzyme of alkane hydroxylase. The are three types of alkane hydroxylase based on the chain length of alkane such as short-chain length/SCL (C2-C4), medium-chain length/MCL (C5-C17), and long-chain length/LCL (C>18). The aims of this study were to characterize and identify alkanes-degrading bacteria from these bacteria. The 30 strains from marine were grown on MCL (Pentane-C5H12, Decane-C10H22, and Pentadecane-C15H32) and LCL (n-Paraffin-C12H19C17 and branch of Pristane-C19H40). The study showed twenty-nine isolates have the ability to degrade alkanes compounds, whereas 14 isolates have grown ability on MCL and LCL medium, 11 isolates have the ability to grow on MCL and n-LCL, 3 isolates have the ability only to grow on MCL medium and 1 isolate has the ability only grow on n-LCL medium. The growth test result indicated that 29 isolates have medium-chain alkane monooxygenase and long-chain alkane hydroxylase. Based on 16S rDNA gene analysis, we obtained twenty nine of oil- degrading bacteria, namely a-proteobacteria (57 %), g-proteobacteria (30 %), Flavobacteria (7 %), Bacilli (3%) and Propionibacteriales (3 %). g-Proteobacteria and a-proteobacteria which seems to play an important role in the alkane biodegradation. National Research and Innovation Agency (BRIN) 2018-06-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4884 10.14203/annalesbogorienses.2018.v22.n1.12-19 Annales Bogorienses; Vol. 22 No. 1 (2018): Annales Bogorienses; 12-19 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4884/3636 Copyright (c) 2018 Ahmad Thontowi, Elvi Yetti, Yopi Yopi https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4885 2026-10-19T03:56:42Z annales:ART

Biological Analysis of Leydig Cells-Conditioned Medium to Support Rat Bone Marrow Mesenchymal Stem Cells Differentiation Kaiin, Ekayanti Mulyawati Prasetyaningtyas, Wahono Esthi Mohamad, Kusdiantoro Djuwita, Ita Yusuf, Tuty Laswardi Setiadi, Mohamad Agus Conditioned medium Leydig cells mesenchymal stem cells 3β-HSD testoterone The developed Leydig cells-conditioned medium (LCM) contains bioactive materials secreted by Leydig cells in vitro.  LCM was used to evaluate the ability of bone marrow mesenchymal stem cells differentiation. Bone marrow mesenchymal stem cells (1x 106 cell/ml) were cultured in : 1) DMEM supplemented with 10% NBCS as a control (M), 2) M supplemented with 10 ng/ml testosterone; 3) M supplemented with 50%  LCM ; 4) M supplemented with 50% LCM and 2.5 IU/ml hCG. Bone marrow mesenchymal stem cells that were cultured with  LCM has a positive reaction (57.4%) to histochemistry staining 3β-HSD and produced 1.87 ng/ml testosterone. Supplementation of hCG to LCM  increased the positive number of Leydig cells and testosterone production by 74.6% and 12.33 ng/ml (P<0.05). It can be concluded that Leydig cells-conditioned medium can support differentiation of bone marrow mesenchymal stem cells into Leydig cells. National Research and Innovation Agency (BRIN) 2018-06-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4885 10.14203/ann.bogor.2018.v22.n1.20-26 Annales Bogorienses; Vol. 22 No. 1 (2018): Annales Bogorienses; 20-26 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4885/3640 Copyright (c) 2018 Ekayanti Mulyawati Kaiin, Wahono Esthi Prasetyaningtyas, Kusdiantoro Mohamad, Ita Djuwita, Tuty Laswardi Yusuf, Mohamad Agus Setiadi https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4887 2026-10-19T03:56:42Z annales:ART

Molecular Detection of Resistance To Bacterial Leaf Blight on Conde Indonesian Rice Variety Fatimah, Fatimah Prasetiyono, Joko Polosoro, Aqwin Baroya, Mushlihatun BTB/POZ domain dominant gene Oryza sativa Xanthomonas oryzae pv. oryzae Xa7 Rice bacterial leaf blight (BLB) disease caused by Xanthomonas oryzae pv. oryzae (Xoo) affected grain yield and decreasing rice production in rice growing countries. Conde, Indonesian rice variety, exhibits high resistance to most of the Indonesian races of (BLB) and has been used in Indonesia for cultivated rice. This study was aimed to conduct the molecular detection between proximal markers in chromosome 6 and relative expression of Conde rice variety compare to IRBB7 in Xa7 region. The population screening, BLB evaluation and molecular detection around the Xa7 region were conducted. The results showed from the collection of individual recombinants between resistant and susceptible parents narrow the region containing the BTBPOZ domain. The sequence alignment of Xa7LD37 in two resistant and three susceptible cultivars demonstrated a perfect association. The sequence alignment in exon region of Loc_Os06g46240 in Nipponbare, IRBB7, and IR64 identified indel/SNPs in this region leading to nucleotide substitution and frameshift resulting in amino acid change between resistant and susceptible cultivars. It was predicted that Conde revealed the similar gene action with Xa7 gene for BLB that encodes a BTB POZ domain. National Research and Innovation Agency (BRIN) 2018-06-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4887 10.14203/ann.bogor.2018.v22.n1.27-34 Annales Bogorienses; Vol. 22 No. 1 (2018): Annales Bogorienses; 27-34 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4887/3641 Copyright (c) 2018 Fatimah Fatimah, Joko Prasetiyono, Aqwin Polosoro, Mushlihatun Baroya https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4888 2026-10-19T03:56:42Z annales:ART

Growth and Proline Content of Irradiated in Vitro Shoots of Ubi Kuning Cassava Genotype Cultured at Different Temperatures Supatmi, Supatmi Rahman, Nurhamidar Hartati, N. Sri Ubi Kuning gamma irradiation temperatures proline growth performances in vitro Cassava (Manihot esculenta) is an important crop to food security under climate change due to its various tolerance mechanism under stress conditions. However, the sustainable growth of cassava in the field depends on many factors especially temperature. The objective of the research was to investigate the growth performances and proline contents of irradiated Ubi Kuning at dosage of 10 Gy, cultured in Murashige Skoog (MS) hormone-free solid medium for 4 weeks at three different temperature treatments i.e 25oC, 28oC and 30oC. Each treatment consisted of 3 clone explants with 5 replicates. Results show that growth performances of irradiated plantlets were better compared to that of non-irradiated plantlets in terms of plant height and number of leaves at all temperature tested. The best growth performances were obtained from irradiated plantlets grown under 30oC. The proline content of irradiated Ubi Kuning was high when they were grown under 25 oC and 30oC, implying that these plantlets had the possibility to tolerant to lower and higher-temperature condition. This study is initially useful to find out the growth ability of irradiated Ubi Kuning in response to lower and higher temperature. National Research and Innovation Agency (BRIN) 2018-06-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4888 10.14203/ann.bogor.2018.v22.n1.35-46 Annales Bogorienses; Vol. 22 No. 1 (2018): Annales Bogorienses; 35-46 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4888/3642 Copyright (c) 2018 Supatmi Supatmi, Nurhamidar Rahman, N. Sri Hartati https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4908 2026-10-19T03:56:42Z annales:ART

Optimization of Substrate and Starter Cell Concentrations for Dibenzothiopene Biodegradation by Indigeneous Marine Bacteria Mauricauda olearia LBF-1-0009, Alcanivorax xenomutants LBF-1-0018, and Stakelama pacifica LBF-1-0031 Yetti, Elvi Thontowi, Ahmad Yopi, Yopi optimization substrate concentration cell concentration dibenzothiophene biodegradation marine bacteria Dibenzothiophene (DBT) and its derivatives have been widely used as model organic sulfur compounds in petroleum, included their biodegradation process. The abilities of microorganisms to degrade pollutants are significantly influenced by various factors such as microbial species, nutrients and environmental parameters. In this research, we carried out further study to determine optimal condition for DBT biodegradation regarding with substrate and strains cell concentration by several indigenous marine bacteria from Indonesia. These three isolates were belong to Mauricauda olearia, Alcanivorax xenomutants, and Stakelama pacifica, with homology result 99% each. Optimal dibenzothiophene as substrate reached by all isolates is 100 ppm, while cell concentration or microbial numbers that gave highest growth for all isolates is 20 based on conversion of OD600 nm measurement. National Research and Innovation Agency (BRIN) 2017-12-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4908 10.14203/ann.bogor.2017.v21.n2.38-44 Annales Bogorienses; Vol. 21 No. 2 (2017): Annales Bogorienses; 38-44 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4908/3645 Copyright (c) 2017 Elvi Yetti, Ahmad Thontowi, Yopi Yopi https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4909 2026-10-19T03:56:42Z annales:ART

Optimization of Somatic Embryogenesis Induction of Cassava (Manihot esculenta Crantz) Susanti, Idha Suharsono, Suharsono Widyastuti, Utut Siregar, Ulfah Juniarti Tjahjoleksono, Aris Manihot esculenta embryogenic callus picloram The embryogenesis (SE) has important role for genetic engineering of cassava (Manihot esculenta Crantz). However, the success of SE induction depend on plant growth regulator s (PGR)s and treatment enriched in induction media.  This experiment tried to induce callus formation of cassava from several in vitro explants: immature leaf, apical bud, and internode; and to develop somatic embryogenesis of cassava in several media enriched with tyrosine and copper sulphate (CuSO4) added into media enrich with picloram as treatment.  Different response of explants source to callus induction treatment from those three varieties in callus induction as well as friable callus formation were found in this experiment. The best medium to induce varied with variety; MS media supplemented 12 mg/L picloram + 0.5 mg/L CuSO4 was the best for “Adira 4” and  half MS and half GD media supplemented 12 mg/L picloram + 100 mg/L tyrosine for “Malang 6”.  All treatments resulted somatic embryo which developed indirectly and in morphologically normal somatic embryos National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4909 10.14203/ann.bogor.2017.v21.n2.45-51 Annales Bogorienses; Vol. 21 No. 2 (2017): Annales Bogorienses; 45-51 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4909/3646 Copyright (c) 2017 Idha Susanti, Suharsono Suharsono, Utut Widyastuti, Ulfah Juniarti Siregar, Aris Tjahjoleksono https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4910 2026-10-19T03:56:42Z annales:ART

Improvement of HER2I655V TARMS-PCR Performance by DNA Quality Analysis Budiarto, Bugi Ratno Azamris, Azamris Desriani, Desriani TARMS-PCR HER2I655V DNase I Polymorphism Reliable TARMS-PCR is a prerequisite in constructing a solid conclusion in genetic diagnostics. The validity of data generated by this molecular technique is hampered by a false positive result. In attempt to develop a  TARMS-PCR for HER2I655V genotyping with no interfering of bias we used DNase I to eliminate DNA contaminant resided in PCR reagent. TARMS-PCR without enzyme treatment using recombinant plasmids that contained HER2I655V gene with represented its alleles was used to evaluate the presence of false positive  result while DNase I treated-PCR reagent was used in TARMS-PCR to evaluate the effective dose of the enzyme and further to adjust the TARMS-PCR conditions.  PCR master mix kit used in this study produced a false positive result on HER2I655V TARMS-PCR as proven by the presence of multiple PCR products in Non-Template Control (NTC) and 0.1 U of the enzyme could eliminate this DNA contaminant effectively, although this pretreatment altered the specificity of HER2I655V TARMS-PCR genotyping on certain genotype. Combination of touchdown TARMS-PCR with another allele-specific primer recovered specificity of detection on this model system. Interestingly, this optimized HER2I655V TARMS-PCR can only be used for genotyping the clinical samples if only further optimization was done using genomic DNA as template National Research and Innovation Agency (BRIN) 2017-12-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4910 10.14203/ann.bogor.2017.v21.n2.52-62 Annales Bogorienses; Vol. 21 No. 2 (2017): Annales Bogorienses; 52-62 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4910/3647 Copyright (c) 2017 Bugi Ratno Budiarto, Azamris Azamris, Desriani Desriani https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4913 2026-10-19T03:56:42Z annales:ART

Pectinase Production and Clarification Treatments of Apple (Malus Domestica) Juice Berutu, Cocok Ana Maryani Fahrurrozi, Fahrurrozi Meryandini, Anja Bacillus clarification apple juice pectinase Pectinases are a group of an enzyme that break down pectin, a polysaccharide that is found in plant cell walls. Today, the application of pectinolytic enzymes plays an important role in food technology for the maceration of fruits and vegetables, including for the extraction and clarification of juice. This research aimed to produce pectinase enzyme for clarifying of apple juice. A microbial culture was selected from cocoa bean fermentation samples and identified as Bacillus sp.. The highest enzyme activity was investigated after 48 hours of incubation. Citrus pectin as the carbon source and peptone as the nitrogen source was found as the best component for pectinase production. The optimum condition of pectinase activity was observed at pH 5, temperature 40 °C and the crude enzyme had the higher activity at one hour storage. Apple juice was treated with the enzyme at different concentrations (0%, 0.5%, 1%, 2%, 4%). Apple juice clarification was evaluated for its percent clarity and viscosity. The result showed that enzyme treatment at 4% in apple juice promoted juice clarification and decreased pH and viscosity. In conclusion, the quality of apple juice can be improved by enzymatic treatment using pectinase. National Research and Innovation Agency (BRIN) 2017-12-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4913 10.14203/ann.bogor.2017.v21.n2.63-68 Annales Bogorienses; Vol. 21 No. 2 (2017): Annales Bogorienses; 63-68 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4913/3649 Copyright (c) 2017 Cocok Ana Maryani Berutu, Fahrurrozi Fahrurrozi, Anja Meryandini https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/4914 2026-10-19T03:56:42Z annales:ART

ILE655VAL Genotyping Study of HER2 - Positive Breast Cancer of Patients from Padang - Indonesia With High Resolution Melting Technique Wulandari, Dwi Azamris, Azamris Nurhayati, Isna Warisman, M. Ali Budiarto, Bugi Ratno Desriani, Desriani Breast cancer HER2 I655V HRM allele Transtuzumab has proven to be a great improvement in the treatment of HER2 + of breast cancer patients, but it is associated with relevant adverse cardiac events and with significantly elevated cost of treatment.  One of the risk factors for cardiotoxicity due to transtuzumab is the I655V HER2 polymorphism (GTC> ATC mutation) in which the allele mutant (Ile val or val / val) has a higher risk than the wild type (Ile/Ile). The detection of specific alleles is very important for therapeutic decision-making. In this study, our group have developed a HRM with EvaGreen dye method to discriminate specific allele of I655V HER2 (wild type, heterozygote mutant or homozygote mutant) in 66 frozen section samples of HER2+ of breast cancer patients. Our group reported the wild type is the most prevalent allele (77,27%), whereas heterozygous mutation is significantly present in this research (21,21%) and only 1,52% of sample detected as minor allele.  It showed that only one sample detected as a minor allele (val/val) and may have relatively low abundance in the population. This method has been compared with sanger sequencing and giving 100% of validity. National Research and Innovation Agency (BRIN) 2017-12-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/4914 10.14203/ann.bogor.2017.v21.n2.69-75 Annales Bogorienses; Vol. 21 No. 2 (2017): Annales Bogorienses; 69-75 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/4914/3650 Copyright (c) 2017 Dwi Wulandari, Azamris Azamris, Isna Nurhayati, M. Ali Warisman, Bugi Ratno Budiarto, Desriani Desriani https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/6574 2026-10-19T03:56:42Z annales:REV

The Emergence of Biosimilars in Indonesia: Guidelines, Challenges, and Prospects Wardiana, Andri Ningrum, Ratih Asmana Biosimilar Indonesia Guidelines Challenges Prospects According to the Food and Drug Administration (FDA), biosimilar is defined as a product which is highly similar to the reference product without clinically meaningful differences in safety, purity and potency. Indonesia is a developing country which has more than 250 million people. In 2008, the world market of biosimilar was USD 100 billions with 10-20% of domestic need from total market. Even though the need is very high, Indonesia still has not been able to produce Biosimilar independently. To stimulate the domestic production on biosimilar, National Agency for Drug and Food Control (NADFC) Republic of Indonesia has assigned Regulation of Biosimilar as Peraturan Kepala Badan Pengawas Obat Dan Makanan Republik Indonesia Nomor 17 Tahun 2015 Tentang Pedoman Penilaian Produk Biosimilar. The guidance covers the quality requirement and evaluation of Biosimilar products. Indonesian Ministry of Health has a strategic plan in biopharmaceutical covering biosimilar, which is going to be developed from 2015 to 2025. The strategy is expected to initiate biosimilar production in Indonesia. This review focuses on the guidelines of biosimilars in Indonesia compared to other international regulatory bodies, as well as the challenges and prospects of biosimilars development.  National Research and Innovation Agency (BRIN) 2016-12-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/6574 10.14203/ann.bogor.2016.v20.n2.37-46 Annales Bogorienses; Vol. 20 No. 2 (2016): Annales Bogorienses; 37-46 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/6574/4914 Copyright (c) 2016 Andri Wardiana, Ratih Asmana Ningrum https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7092 2026-10-19T03:56:42Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita The editors would like to announce that, in the years of 2002 and 2003, The Research Centre for Biotechnology The Indonesian Institute of Sciences (LIPI) did not publish Annales Bogorienses New Series due to some management problems. The last issue waspublished with Volume 8, Number 2, 2001. Under new editors, in this year of 2004, continuing the previous volume, this journal is published again starting with Volume 9, Number 1. In this ninth edition of Annales Bogorienses, we would also like to introduce anew look of this journal to our readers. We will I continue to publish this journal periodically twice a year (in June and December).Many thanks to all authors for their efforts, attentions, and time throughout the editing process that make this current edition become possible. Tolerance of our editorial judgments is much appreciated. We encourage our readers to submit original papers on topics of interest to life sciences researchers. June, 2004M. Ahkam SubrotoChief Editor National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7092 10.14203/annalesbogorienses.2004.v9.n1.i Annales Bogorienses; Vol. 9 No. 1 (2004): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7092/5368 Copyright (c) 2004 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7093 2026-10-19T03:56:42Z annales:ART

Sensitivity Improvement of A Direct Competitive Elisa for Atrazine by Exploiting Low Cross-Reactivity of An Atrazine-Specific Recombinant Antibody Fab-Fragment Kusharyoto, Wien Schmid, Rolf D. atrazine direct-competitive ELISA cross-reactivity recombinant Fab-fragment structural modeling The hapten-binding site of the antibody Fab-fragment K411B specific towards the herbicide atrazine (2-chloro-4-(ethylamino)-6-(isopropyJamino)-1,3,5-triazine) was modified by means of structural modeling and site-directed mutagenesis. A triple mutant (GlnL89Glu/ValH37Ile/GluL3Val) of the Fab-fragment showed an increased affinity towards the hapten H/Cl/C6 (4-amino-6-chloro-l,3,5-triazine-2-(6-aminohexanecarboxylic acid) compared to the affinity of the wild-type Fab-fragment towards the same hapten. However, the mutant exhibited substantially lower affinity towards the hapten H/Cl/C6 than towards atrazine and the hapten iPr/Cl/C6 (4-chloro-6-(isopropylamino)-1,3,5-triazine-2-(6-aminohexanecarboxylic acid), which is usually used in the synthesis of enzyme tracers in ELISA for atrazine. Advantage was taken of the low cross-reactivity and increased affinity of the mutant Fab fragment towards H/Cl/C6 to improve the sensitivity of a direct-competitive ELISA tor atrazine. H/Cl/C6 was covalently conjugated with horseradish peroxidase (HRP), and the conjugate H/CI/C6-HRP was used as enzyme tracer in the ELISA for atrazine. An eight-fold improvement in sensitivity of a direct-competitive ELISA for atrazine could be achieved using the tracer H/Cl/C6-HRP compared to the sensitivity of ELISA using the tracer iPr/Cl/C6-HRP. The detection limit for atrazine was as low as 0.01 i g/l. National Research and Innovation Agency (BRIN) 2004-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7093 10.14203/ann.bogor.2004.v9.n1.1-8 Annales Bogorienses; Vol. 9 No. 1 (2004): Annales Bogorienses; 1-8 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7093/5369 Copyright (c) 2004 Wien Kusharyoto, Rolf D. Schmid https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7094 2026-10-19T03:56:42Z annales:ART

An Application of Reverse Transcriptase Polymerase Chain Reaction in A Relative Quantification of Gene Expression Santoso, Adi RT-PCR Northern blot NPA gene expression quantification The ability to quantify steady state levels of individual messenger-RNA (mRNA) transcripts has been the key issue for study on the control of gene expression. Although two available techniques, Northern blot and nuclease protection assays (NPA) have been widely used tor detecting mRNA, these techniques have critical limitations. The most obvious limitation of these two techniques is the required number of target mRNAs to be detected. Reverse transcription-polymerase chain reaction (RT-PCR), which has been accepted as a highly sensitive and specific method, provides a means for detecting and quantifying gene expression using, theoretically only a single molecule of mRNA. The sensitivity and reliability of RT-PCR is dependent upon both the RT and PCR steps. The PCR step has been problematic because of the exponential nature of this reaction where small variation can lead to dramatic changes in final result. Therefore, the use of RT-PCR for quantification of gene expression requires pre-experimental planning and design. In this experiment, the procedure for pre-experimental planning, linear range determination and subsequent relative quantification of gene expression are described in detail. A study of ornithine decarboxyJase gene, a gene involved in the polyamine biosynthesis and temporally expressed, during embryogenesis of Musca domestica (housefly) was used as the model. The results show that during early embryogenesis (t-1 to t-4) the expression level was very low. The increase in expression profile was observed started at t-5, peaked at t-9, and followed by substantial decrease from t-10 to t-12. National Research and Innovation Agency (BRIN) 2004-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7094 10.14203/ann.bogor.2004.v9.n1.9-19 Annales Bogorienses; Vol. 9 No. 1 (2004): Annales Bogorienses; 9-19 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7094/5370 Copyright (c) 2004 Adi Santoso https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7095 2026-10-19T03:56:42Z annales:ART

Maternal Contribution in Revealing The Effects of Methoxyacetic Acid (MAA) Administered Before Implantation on The Embryonic Development of Swiss Webster Mice (Mus musculus) Kaiin, Ekayanti M Sumarsono, Sony H. Surjono, Tien W. Sudarwati, Sri methoxyacetic acid (MAA) preimplantation embryo donor recipient embryo transfer mice Maternal contribution to and direct action of methoxyacetic acid (MAA) on the embryonic development bad been examined by conducting embryo transfer. To reveal the maternal contribution, compacted morulae and early blastocysts, which were collected from untreated Swiss Webster donor mice on day 3 of gestation, were transferred to day 2 pseudopregnant recipients, after having been treated with 2.0 mmo/lkg body weight (b.w.) MAA by gavage on day 1 of pseudopregnancy. Direct effect of MAA on the embryonic development were observed by transferring compacted morulae and early blastocysts, similarly recovered from day 3 pregnant donor mice, after MAA treatment on day 2 of gestation with the same method and dosing, to untreated day 2 pseudopregnant recipients. Control donor mice and recipient were given distilled water only as the MAA solvent. Observations on fetuses resulting from embryo transfer wert: carried out on day 16 of gestation . Administration of MAA to the donors tended to decrease the unplantatlon rate and the survival rate of the implanted embryos. When MAA was given to the recipients the implantation rate and survival rate of embryos transferred decreased significantly (p<0.05) but the survival rate of implanted embryos were significantly higher (p<0.05) Lf compared to those of MAA treated donors. The intrauterine death tended to inc rease either in the treated donors or recipients. There was no effect of MAA on the fetal body weight and in producing fetal malformations. It is concluded that at the beginning of implantation, maternal contribution in revealing the effects of MAA on the embryonic development of Swiss Webster mice is predominant, whereas after implantation took place, the quality of the embryos become more important for their survival.  National Research and Innovation Agency (BRIN) 2004-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7095 10.14203/ann.bogor.2004.v9.n1.20-26 Annales Bogorienses; Vol. 9 No. 1 (2004): Annales Bogorienses; 20-26 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7095/5371 Copyright (c) 2004 Ekayanti M Kaiin, Sony H. Sumarsono, Tien W. Surjono, Sri Sudarwati https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7096 2026-10-19T03:56:43Z annales:ART

The Use of 16S rRNA and nodC Gene Sequence in Resolving The Phylogenetic Relationship of Rhizobia Associated With Paraserianthes falcataria (L.) Nielsen Plant Prana, Titik K Sone, Teruo Kawasaki-Nakagawa, Hiroko Yokota, Atsushi Seki, Tatsuji Tomita, Fusao nodC gene 16S rRNA gene rhizobia phylogenetic tree Studies on genetic position of several isolates living symbiotically on Paraserianthes falcataria have been carried out using amplification and sequencing techniques of 16S rDNA and specific gene for nodulation. The phylogenetic tree constructed based on 16S rDNA showed that rhizobia growing symbiotically on Paraserianthes falcataria consisted of 3 groups. The first group, was fast growing rhIzobia which have close relationship to Rhizobium tropicii, the second and the third groups were slow-growing rhizobia which have close relationship to Bradyrhizobium elkanii and Bradyrhizobium japonicum. However, the phylogenetic tree constructed on the basis of partial nodC gene indicated the existence of an independent group, since they did not show any significant degree of relationship with the existing groups. This was also supported by differences in physiological characteristic i.e Indole Acetic Acid production and salt tolerance of the isolates. These differences shows us that direct sequencing method of certain specific genes could give a more specific result than would display more clearly the degree of relationship at species level. National Research and Innovation Agency (BRIN) 2004-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7096 10.14203/ann.bogor.2004.v9.n1.27-36 Annales Bogorienses; Vol. 9 No. 1 (2004): Annales Bogorienses; 27-36 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7096/5372 Copyright (c) 2004 Titik K Prana, Teruo Sone, Hiroko Kawasaki-Nakagawa, Atsushi Yokota, Tatsuji Seki, Fusao Tomita https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7097 2026-10-19T03:56:43Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita The editors would like to announce that, in the year of 2002 and 2003, The Research Centre for Biotechnology, The Indonesian Institute of Sciences (LIPI) did not publish Annales Bogorien es New Series due to some management problems. The last issue was published with Volume 8, Number 2, 2001. Under new editors, in this year of 2004, continuing the previous volume thi journal is published again starting with Volume 9, Number 1. In this ninth edition of Annales Bogorienses, we would also like to introduce a new I ok of this journal to our readers. We will continue to publish this journal periodically twice a year (in June and December).Many thank to all the author for their efforts, attention and time throughout the editing process that make this current edition become possible. Tolerance of our editorial judgment is mu h appreciated. We encourage our readers to submit original papers on topics of interest to life sciences researcher . December, 2004M. Ahkam Subroto Chief Editor National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7097 10.14203/annalesbogorienses.2004.v9.n2.i Annales Bogorienses; Vol. 9 No. 2 (2004): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7097/5373 Copyright (c) 2004 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7098 2026-10-19T03:56:43Z annales:ART

Modification of Plasmids and Cry Genes of Bacillus thuringiensis subsp. kurstaki HD-I After Treatment With Ethylmethanosulfonate (EMS) and UV Light Jusuf, Eddy UV and EMS mutagenesis autolysin deficient mutant plasmid cry genes Bacillus thuringiensis subsp. kurstaki HD-1 Bacillus thuringiensis subsp. kurstaki HD-1 is a potential insecticidal bacterium producing five type of a-endotoxin crystal proteins. This bacterium is widely commercialized due to its wide spectrum toxicity against both Lepidopteran and Dipteran larvae. The objective of this work was to create autolysin deficient mutant causing cell fails to lyse. This mutant yield intact cells within spore and a-endotoxin crystal protein protected inside, from which an undamaged active bio-insecticide would be obtained. Two methods of mutagenesis, 2% of ethylmethanosulfonate and 10, 25, and 50 second of UV light exposure, resulted in mot (loss of motility) mutation. Observation showed that 14 of 20 survived mutants have lost some of its plasmids (varied from one to five). while the other six maintained their plasmids. By employing the polymerase chain reaction (PCR) the change on the cry genes was studied. National Research and Innovation Agency (BRIN) 2004-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7098 10.14203/ann.bogor.2004.v9.n2.39-49 Annales Bogorienses; Vol. 9 No. 2 (2004): Annales Bogorienses; 39-49 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7098/5374 Copyright (c) 2004 Eddy Jusuf https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7099 2026-10-19T03:56:43Z annales:ART

Cytological Analysis of Root Cultures of Artemisia cina Ermayanti, Tri Muji Yanti, Oktavia Hafiizh, Erwin Al untransformed roots transformed roots chromosome number genetic stability Artemisia cina Artemisia cina is a medicinal plant species producing bioactive compounds which are potential a antitumor, antifungal and antibacterial. The aim of this study was to analyze the stability of chromosome number in root cultures of A. cina. Transformed root culture was established by infection of leaves of A. cina with Agrobacterium rhizogenes strains 07-20001, ATCC 15834, A4; and Agrobacterium tumefaciens strain R1000. Roots isolated from glasshouse plants, plantlets grown in solid and liquid MS medium were utilized for investigation of chromosome examination of untransformed roots. Chromosome examination was conducted by squashing method and chromosome numbers were calculated under microscope. The results showed that both untransformed and transformed root had in lability in the chromosome number, but had the modal number of chromosome x=8 with the diploid number of 2n =4x = 32. Roots isolated from glasshouse plants of A. cina had 53.7~ of cell with the diploid numbers of 2n = 3 and 46.3% of cells had chromosome numbers ranged from 2n = 12 to 2n = 64. Untransformed roots isolated from plantlets cultured in solid medw had only 36.1% or cells with chromo orne number of 20 = 32, and untransformed roots grown in liquid medium had 49.4% of cells with 2n =32. The chromosome numbers of A. cina transformed roots was affected by trains of Agrobacterium. Root transformed with the bacterium Strain 07-20001 showed the highest in normal chromosome number of 2n = 32 (62.4%) followed by roots transformed with strains ATCC 15834 (61.9%). R1000 (43.6%) and A4 (43.0%). The range of the chromosome number of untransformed roots was from 2n=17 to  2n=64 whilst that of transformed roots was from 2n=11 to 2n=66. National Research and Innovation Agency (BRIN) 2004-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7099 10.14203/ann.bogor.2004.v9.n2.50-58 Annales Bogorienses; Vol. 9 No. 2 (2004): Annales Bogorienses; 50-58 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7099/5375 Copyright (c) 2004 Tri Muji Ermayanti, Oktavia Yanti, Erwin Al Hafiizh https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7100 2026-10-19T03:56:43Z annales:ART

Denitrification of Activated Sludge in The Presence of Different Organic Substrates Agustiyani, Dwi Yamagishi, Takao Denitrifying microorganism denitrification nitrogenous oxide activated sludge The effect of organic carbon denitrifying activity was studied in batch reactor. Fom reactors were operated in parallel under anoxic condition in four different donor electrons. which were acetic acid (Reactor A), methanol (Reactor M), phenol (Reactor P), and glucose (Reactor G). The reactors were fed with the artificial waste, which contain 721.8 mg/l NaNO3. The concentration organic carbon added to the reactors were varied from TOD:N ratio of 0.5:1; 1: 1, 1.5:1, to 2:1. The denitrification activity was estimated by measuring the reduction rate of nitrogenous oxide and N2O gas production. The denitrification capacity of adapted-sludge was also investigated, and the rates were estimated from the cumulative N2O (without acetylene inhibition) and N2 gas production. Reduction rate of nitrogenous oxide in all reactors increased during the investigation; lhe increase reduction rate were correlated to the increase of organic carbon concentration. The maximum reduction rate of nitrogenous oxide in Reactor A was higher than those of the others. However, reduction rate in Reactor M was more constant, so that nitrogenous oxides existed in this reactor was removed faster. The highest potential denitrification rate (N20 production) was observed in sludge of Reactor A. However, N2 gas recovery trom nitrate and nitrite transformed by sludge of Reactor M was the highest. Linear correlation between nitrogenous oxide reduction with gas production was observed in Reactor A, M and P, but not in Reactor G. National Research and Innovation Agency (BRIN) 2004-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7100 10.14203/ann.bogor.2004.v9.n2.59-71 Annales Bogorienses; Vol. 9 No. 2 (2004): Annales Bogorienses; 59-71 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7100/5376 Copyright (c) 2004 Dwi Agustiyani, Takao Yamagishi https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7101 2026-10-19T03:56:43Z annales:ART

Development of Somatic Embryo in Lithospermum erythrorhizon Siebb. et Zucc and The Study on The Effect of Methyl Jasmonate on its Maturation Mariani, Totik Sri Ramayanti, Octavia Yazaki, Kazufumi Miyake, Hiroshi Somatic embryogenesis Lithospermum erythrorhizon methyl jasmonate A research on the somatic embryogenesis in Lithospermum erythrorhizon has been conducted. Embryogenic callus was inoculated in EIM9 liquid medium (modification of LS medium), i.e. L2PVP (initiation medium) and the development of somatic embryo was observed. Ten uM and 100 uM methyl jasmonate was added into L3PVP medium (differentiation medium) for somatic embryo maturation. The purposes of this research were to observe the development of somatic embryo and to observe the effect of methyl jasmonate on maturation of the somatic embryo in L. erythrorhizon. The results showed that somatic embryogenesis in L. erythrorhizon derived from single cells differentiated further forming proembryo, globular heart, torpedo and cotyledon stage. Treatment with 10 uM and 100 uM methyl jasmonate induced maturation of somatic embryos (cotyledon stage) after transferring them to embryo development medium (L4PVP). National Research and Innovation Agency (BRIN) 2004-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7101 10.14203/ann.bogor.2004.v9.n2.72-79 Annales Bogorienses; Vol. 9 No. 2 (2004): Annales Bogorienses; 72-79 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7101/5377 Copyright (c) 2004 Totik Sri Mariani, Octavia Ramayanti, Kazufumi Yazaki, Hiroshi Miyake https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7102 2026-10-19T03:56:43Z annales:ART

PCR Amplification of Ornithine Decarboxylase (ODC) Gene Fragment from Tobacco (Nicotiana tabacum L.) cv. Temanggung Djajanegara, Ira Pambudi, Sabar Lestari, Retno Artanti, Nina ornithine decarboxylase tobacco PCR In order to create an antisense construct of the gene encoding Ornithine Decarboxylase (ODC) from tobacco (Nicoticum tabacum L.) cv. Temanggung, the target gene must be isolated. In this paper. we present the PCR amplification of a fragment from putative gene encoding ODC from tobacco cv. Temanggung. Leaf genomic DNA was isolated and used as the template for PCR. PCR optimization was done by adjusting the annealing temperature and the cycle number. Verification of the fragment obtained was also done using the second primer pairs. National Research and Innovation Agency (BRIN) 2004-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7102 10.14203/ann.bogor.2004.v9.n2.80-86 Annales Bogorienses; Vol. 9 No. 2 (2004): Annales Bogorienses; 80-86 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7102/5378 https://ejournal.brin.go.id/annales/article/view/7102/5379 Copyright (c) 2004 Ira Djajanegara, Sabar Pambudi, Retno Lestari, Nina Artanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7104 2026-10-19T03:56:43Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7104 10.14203/annalesbogorienses.2005.v10.n1.i Annales Bogorienses; Vol. 10 No. 1 (2005): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7104/5381 Copyright (c) 2005 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7105 2026-10-19T03:56:43Z annales:ART

Investigation of Functional Difference between TecIII and TecIV in Mammals COS-1 Cells Using GFP Fusion Proteins Atmosukarto, Ines I. C. Booker, Grant W, tyrosine kinase Tec GFP fusion Signal transduction cascades are critical components of intra-and inter-cellular communication. Key component of such cascades includes tyrosine kinases. One such family of tyrosine kinase is the Tec family of tyrosine kinases. This family of tyrosine kinases is expressed mainly in cells of the hematopoietic lineage, and mutations in at least one of its one member of this family, Btk, has so far been associated with the human immunodeficiency disorder X-Linked Agammaglobulinemia. Two major isoforms of the Tec transcript, referred to as TecIII and TecIV have been detected in various mouse embryonic and adult tissues, as well as in a number of different hematopoietic cell lines: TecIV is the full length Tec protein with functional PH, TH, SH3, SH2, and Kinase domains, while TecIII is gen rated by the splicing out of exon 8 sequences to yield a shorter peptide with a non-functional SH3 domain. Using GFP-TecIII fusion proteins, this shorter isoform of Tec was shown to have biological characteristics that differed from TecIV. National Research and Innovation Agency (BRIN) 2005-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7105 10.14203/ann.bogor.2005.v10.n1.1-7 Annales Bogorienses; Vol. 10 No. 1 (2005): Annales Bogorienses; 1-7 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7105/5382 Copyright (c) 2005 Ines I. C. Atmosukarto, Grant W, Booker https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7154 2026-10-19T03:56:43Z annales:ART

Identification of Degradation Pathway of Vinyl Acetate Using Bacterial Isolate V2 and Characterization of The Involved Enzymes Soenarko, Bambang Sulistinah, Nunik Nieder, Maria Meyer, Ortwin vinyl acetate microbial degradation vinyl acetate-esterase aldehyde dehydrogenase alcohol dehydrogenase bacterial isolate V2 Vinyl acetate is a toxic substance, but has a high commercial value. In this study we show that vinyl acetate is subject to microbial degradation at rates of up to 6.38 and 1 mmol 1 per g (dry weight) under aerobic and anaerobic conditions, respectively. It was hydrolyzed by bacterium V2 to ethanol, acetaldehyde and acetate. The enzymes involved in the metabolism of vinyl acetate were vinyl acetate esterase, aldehyde dehydrogenase, and alcohol dehydrogenase, which localized in the cytoplasmic fraction. The Km values of vinyl acetate esterase and alcohol dehydrogenase were 6.13 mM and 0.24 mM. respectively. Vinyl acetate esterase hydrolyzed the ester to acetate and vinyl alcohol. The latter isomerized spontaneously to acetaldehyde and was then converted to acetate. The acetaldehyde was disproportionated into ethanol and acetate. The acetate was then converted to acetyl coenzyme A and oxidized through the tricarboxylic acid cycle and the glyoxylate bypass. National Research and Innovation Agency (BRIN) 2005-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7154 10.14203/ann.bogor.2005.v10.n1.8-14 Annales Bogorienses; Vol. 10 No. 1 (2005): Annales Bogorienses; 8-14 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7154/5415 Copyright (c) 2005 Bambang Soenarko, Nunik Sulistinah, Maria Nieder, Ortwin Meyer https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7155 2026-10-19T03:56:43Z annales:ART

Construction of A Recombinant Virus Between Poliovirus and Coxsackie A Virus 11 Utama, Andi Shimizu, Hiroyuki poliovirus recombination CAV-11 Recent outbreaks of circulating vaccine-derived poliovirus (cVDPV) revealed the possibility of recombination between vaccine strains poliovirus (PV) and cluster C enterovirus. Based on genetic analysis, it is assumed that coxsackie A virus 11 (CAV-11), one f the cluster C enterovirus, may naturally recombine with PV. To elucidate this hypothesis, the chimeric virus between PJ156, a type I cVDPV isolate isolated from an acute flaccid paralysis (AFP) case in the Philippines in 2001, and CAV-11 (PJ156/CAV-11) was constructed by using long-PCR method. As the result , PJ156/CAV-11 was viable in HEp-2 cell line. The PJ156/CAV-11 exhibited mostly similar phenotype with parental PJ156 in term of plaque size, viral growth and neuroviruJence. These results suggested that recombination between PV and CAV-11 might naturally occur during transmission of vaccine strains in the community. The effect of recombination on the viral phenotype is significantly depending on the counterpart virus. National Research and Innovation Agency (BRIN) 2005-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7155 10.14203/ann.bogor.2005.v10.n1.15-22 Annales Bogorienses; Vol. 10 No. 1 (2005): Annales Bogorienses; 15-22 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7155/5416 Copyright (c) 2005 Andi Utama, Hiroyuki Shimizu https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7157 2026-10-19T03:56:43Z annales:ART

Subcloning, Expression, and Characterisation of A Recombinant Antibody Fab-Fragment Specific Towards 2,4-D Kusharyoto, Wien 2,4-D antibody competitive ELISA Fab-fragment IMAC A generic strategy was established for subcloning the VH and VL gene of antibody variable domains into the piasmid pASK85 for the expression of Fab antibody fragments. pASK85 bear coding sequences for murine constant domains including a His6-tag at the carboxy-terminal end of the constant heavy-chain domain. The VH and YL gene derived from the monoclonal antibody E2/B5 specific towards 2,4-dichlorophenoxyacetic acid (2,4-D) were used in this study. Escherichia coli was used as host cells for the biosynthesis of the Fab-fragment. The Fab-fragment was subsequently purified from the periplasmic extract in a single step by immobilised metal-ion affinity chromatography (IMAC). The production level obtained were 0.5-0.8 mg purified Fab-fragments per liter E. coli culture. The sensitivity and cross-reactivity of the Fab-fragment determine by direct competitive ELISA were similar to those of the parental monoclonal antibody E2/B5. National Research and Innovation Agency (BRIN) 2005-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7157 10.14203/ann.bogor.2005.v10.n1.23-30 Annales Bogorienses; Vol. 10 No. 1 (2005): Annales Bogorienses; 23-30 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7157/5418 Copyright (c) 2005 Wien Kusharyoto https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7158 2026-10-19T03:56:43Z annales:ART

The Effect of Honey on Bacterial Growth, Protein Degradation, Amino Acids Contents, and Volatile Compounds of Milks at Storage Khusniati, Tatik Widyastuti, Yantyati pa teurized milks skim honey protein aminoacids volatile compounds Pasteurized milks spoiled at refrigerated storage due to growth of psychrotrophic bacteria. Honey which contain antibacterial and aromatic compounds may be used as supplement to inhibit psychrotrophic bacterial activities. To know nutritional and flavor compounds of milks with and without honey, effect of honey on bacterial growth protein degradation, amino acids, and volatile compounds of stored milk were detected. Bacterial growth, protein degradation, amino acids, and favor compounds were detected by total plate counts. formol titration, HPLC, and GCMS, respectively. The results how that bacterial growth and protein degradations in honey milks were lower than that without honey. Bacterial growth (5.2x103 -9.3x106 cfu/mL) and protein degradation (2.37-2.59%) in honey whole milks were lower than that (6.2x104-6.5x107 cfu/mL) (2.54-2.88%) in skim milks, respectively P<0.05). At 10 days after use by date, changing between amino acids contents in whole milks with and without honey were more significant than that or skim milk (P<0.05); and volatile compounds percentages in honey whole milks were higher than that without honey. While that in honey skim milks vice versa. Honey caused decreasing bacterial growth and protein degradation, changing amloo a id 'contents and producing volatile compounds of stored milk, and honey whole milk were better than honey skim milks. National Research and Innovation Agency (BRIN) 2005-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7158 10.14203/ann.bogor.2005.v10.n1.31-39 Annales Bogorienses; Vol. 10 No. 1 (2005): Annales Bogorienses; 31-39 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7158/5419 Copyright (c) 2005 Tatik Khusniati, Yantyati Widyastuti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7173 2026-10-19T03:56:43Z annales:ART

Agrobacterium-Mediated Transformation of Javanica Rice Plants With A Cry1b Gene Under The Control of Wound-Inducible Gene Promoter Estiati, Amy Rachmawati, Syamsidah Astuti, Dwi Loedin, Inez Hortenza Slamet Agrobacterium cry1B gene wound inducible gene promoter yellow stem borer A cry1B synthetic gene of Bacillus thuringiensis has been used for the transformation of the javanica rice plants cv. Rojolele to confer resistance to an important pest yellow stem borer (Scirpophaga incertulas). Embryogenic callus were co-cultivated with the EHA105 strain of Agrobacterium tumefaciens harbouring binary vector pCAMBIA I301 containing cry1B gene under the control of wound inducible gene promoter (mpi), hygromycin resistance gene (hpt) as a selectable marker and intron-containing b-glucuronidase (gus intron) gene as a reporter gene driven by CaMV35S promoter. Previously. our histochemical assay and PCR analysis had proved the integration of cry1B gene into the genome of rice plants at first generation. However, the existence of the gene should remain stable throughout generation. In this study, the presence of the cry1B transgene in rice transgenic plants at second generation was confirmed by Polymerase Chain Reaction (PCR). Insertion of the cry1B gene in the genome of PCR positive plants was verified by Southern blot analysis and showed that integration of cry1B into the genomic DNA of javanica rice plants cv. Rojolele. An effective resistance of transgenic plants against stem borer was verified in bioassays. National Research and Innovation Agency (BRIN) 2007-06-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7173 10.14203/ann.bogor.2007.v11.n1.1-5 Annales Bogorienses; Vol. 11 No. 1 (2007): Annales Bogorienses; 1-5 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7173/5431 Copyright (c) 2007 Amy Estiati, Syamsidah Rachmawati, Dwi Astuti, Inez Hortenza Slamet Loedin https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7179 2026-10-19T03:56:43Z annales:ART

Motif II of Japanese Encephalitis Virus NS3 Protein is Not Essential for RNA Binding Activity Utama, Andi Shimidzu, Hiroyuki Japanese encephalitis RNA binding motif The role of motif II (DEAH; Asp285-Glu286-Ala287-His288) of Japanese encephalitis virus (JEV) NS3 protein on RNA binding activity was studied. A point mutation was introduced to the motif and the RNA binding activity of each mutant protein was analyzed. Truncated form of each protein with a His-tag was expressed m Escherichia coli BL21(DE3)pLysS and purified by metal affinity resin. Asp-285 and Glu-286 was respectively substituted with Ala. Ala-287 was replaced by Cys, Gly, or Ser. His-288 was mutated to other 19 amino acids. In total, 24 mutant proteins were produced and analyzed. As results, all mutants showed quite similar RNA binding activity indicating that motif II of JEV NS3 is not related to RNA binding activity. The same finding was reported for hepatitis C viruc (HVC) NS3 protein, suggesting lhe similar structure of NS3 protein in the flavivirus. National Research and Innovation Agency (BRIN) 2007-06-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7179 10.14203/ann.bogor.2007.v11.n1.15-21 Annales Bogorienses; Vol. 11 No. 1 (2007): Annales Bogorienses; 15-21 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7179/5434 Copyright (c) 2007 Andi Utama, Hiroyuki Shimidzu https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7183 2026-10-19T03:56:43Z annales:ART

Identification and Activity of The Retrotranposon Tos17 in Indonesian Javanica Rice CV. Rojolele and Japonica Rice CV. Gajahmungkur Nugroho, Satya Loedin, Inez Hortenza Slamet Ouwerkerk, Pieter B. F. Oryza sativa rice javanica retrotransposon Tos17 Retrotransposons are mobile genetic elements that transpose via an RNA intermediate that is revers transcribed before integration into a new location within the host genome. They are ubiquitous in eukaryotic organisms and constitute a major portion of the nuclear genome (often more than half of the total DNA) in plants. Tos17 is a rice endogenous retrotransposon that has been studied thoroughly. Tos17 has been shown to be an efficient insertional mutagen and saturation mutagenesis tool for gene tagging and functional genomics in Japonica rice cv nipponbare. In Javanica rice, however, the presence and activity of Tos17 has not been described thus far. while in some Indica rice Tos17 has been found to be inactive. Javanica rice, also known as tropical Japonica rice, has many cultivars which may serve as potential genetic resources of great interest for breeding programmes. Here, the presence and activities of retrotransposon Tos17 in Javanica rice cv Rojolele was described and compared to those of Japonica rice and Japonica rice cv Gajahmungkur. We identified five and three copies of Tos17 in Rojolele and Gajahmungkur, respectively, with different activities. National Research and Innovation Agency (BRIN) 2007-06-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7183 10.14203/ann.bogor.2007.v11.n1.23-29 Annales Bogorienses; Vol. 11 No. 1 (2007): Annales Bogorienses; 23-29 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7183/5437 Copyright (c) 2007 Satya Nugroho, Inez Hortenza Slamet Loedin, Pieter B. F. Ouwerkerk https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7185 2026-10-19T03:56:43Z annales:REV

Present Status and Challenges on Agricultural Microbe Bio-Prospecting in Indonesia Sukara, Endang Food security is one of the essential needs which can never be ignored by any society, including in particular Indonesia, as the world population is expected to rise to around 10 billion marked by 2025. The problem is that the agriculture in developed and developing countries that follow the path of developed countries experiences create a range of serious environmental degradation. An intensive agricultural production through the use of pesticide and herbicides, forest clearing and continued use of land resources for the agricultural purposes are not sustainable practices. In consequences, degradation of ecosystem, oil erosion, and low productivity of land, poor water quality and lost of biological diversity (including microbial and genetic resources) occurred at alarming state. Meanwhile, the efforts in addressing the above situation is traditionally focused on plant phen types (breeding. plant genetic engineering,  etc.) but the important role of microbial communities that interact with plants to influence plant health and productivity has been largely ignored. The interaction of microbes with plants and its relevant to soil fertility and productivity, the biocontrol properties of microbes and challenges toward sustainable agriculture is highlighted. National Research and Innovation Agency (BRIN) 2007-06-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7185 10.14203/ann.bogor.2007.v11.n1.31-39 Annales Bogorienses; Vol. 11 No. 1 (2007): Annales Bogorienses; 31-39 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7185/5445 Copyright (c) 2007 Endang Sukara https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7209 2026-10-19T03:56:43Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita In this issue, 5 original research papers are presented. The fIrst paper explains the authors attempts to purify and characterize an extracellular enantio seJective lipase from Aspergillus niger, an important component for the manufacture of c1ural drugs such asibuprofen. The second papers describe the identification of a highly abundant lectin in Arabidopsis thaliana. with potential application for biopesticides against nematode. In the third paper, the authors explain an association of a polymorphism in the a-lactoglobulingene on milk yield and quality in local sheep. The fourth paper describes the authors efforts to construct a synthetic human errythropoietin (EPO) in an attempt to obtain high-yield production of the protein in yeast. And the fifth papers describes the newly develop a method to do micropropagation of buah merah (Pandanus conoideus Lam), which is a potentially important source for anticancer medication. The Editor National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7209 10.14203/annalesbogorienses.2008.v12.n1.i Annales Bogorienses; Vol. 12 No. 1 (2008): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7209/5453 Copyright (c) 2008 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7210 2026-10-19T03:56:43Z annales:ART

Rapid Purification and Partial Characterization of an Extracellular Enantioselective Lipase from Aspergillus niger Malle, Domininggus Garingan, Gina Peralta, Milagros Revilleza, Maria Jamela lipase Aspergillus niger purification enantioselectivity chiral drug ibuprofen Lipases [EC. 3. 1.1 .3] are capable of hydro lyzing ester bonds of triglycerides and have received much attention from protein researchers after these enzymes have shown potential applications such us, in the manufacture of commercially important crural drugs due to their enantioselectivity properties. A novel extracellular lipase produced by Aspergillus niger was purified and partially characterized. Using the culture medium as crude extract, the Lipase was purified to 25 .7 folds after Ultrafiltration and DEAE ion exchange chromatography. The enzyme was characterized to have optimum activity at neutral pH and at 30-35°C, while its kinetic parameters, Vmax and Km, were determined to be 1.80 U/mL/min and 0.2 mL, respectively. SDS-PAGE analysis revealed the presence of at least two bands (±87 and ±72 kDa), while native PAGE showed a single band. The two bands could represent subunits in a complex or isoforms of the enzyme. The use of the enzyme in the hydrolysis of a racemic mixture of 2-arylpropionic butyl ester analyzed after HPLC demonstrated an apparent predominant enantioselectivity for the S (+) enantiomer. Thus, this lipase is a promising enzyme for the chiral drug preparation of single enantiomer of 2-arylpropionic acid (ibuprofen). National Research and Innovation Agency (BRIN) 2008-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7210 10.14203/ann.bogor.2008.v12.n1.1-8 Annales Bogorienses; Vol. 12 No. 1 (2008): Annales Bogorienses; 1-8 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7210/5454 Copyright (c) 2008 Domininggus Malle, Gina Garingan, Milagros Peralta, Maria Jamela Revilleza https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7211 2026-10-19T03:56:43Z annales:ART

A Highly Abundant Lectin Protein in Arabidopsis thaliana Confers Resistance Against Pathogens Omega, Maria Prihtamala Thomas-Hall, Skye Schenk, Peer Kobe, Bostjan Arabidopsis thaliana lectin's structure and function pathogen resistance Lectins are glycoproteins that recognize and bind to specific carbohydrates. They are involved in a range of biological functions, such as plant defence, storage proteins seed germination, and plant microbe interactions. Lectin 3.1 (At3g15356) is a protein in plant model, Arabidopsis thaliana, that has been shown to be up-regulated in all defence pathways especially in response to methyl ester jasmonale (MJ). All that was known about the gene was that it had go d homology to the beta domain of legume lectins. That aim of this project was to characterize the structure and function of the lectin protein using CD spectra and X-ray crystallography. AT-DNA insertion line for the lectin gene and a number of 35S over-expression line that had varying levels of expression had been generated, but none of these showed any obvious phenotype. Two protein bands were observed on comassie stained SDS-PAGE gels in the over-expression lines and in MJ induced wild-type (WT). The two protein bands represented two isoforms of the lectin 3.1 protein; in a glycosylation assay the larger protein band was shown to be heavily glycosylated. A nematode (M. incognita) disease assay discovered that the lectin overexpression lines had les nematode eggs compared to that of the WT and that the insertion line bad more nematode eggs than the WT. This data provide evidence that lectin 3.1 improves plant resistance against M. incognita infection. interestingly, the nematode gut lining contains fucose with which lectin 3.1 binds to. National Research and Innovation Agency (BRIN) 2008-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7211 10.14203/ann.bogor.2008.v12.n1.10-15 Annales Bogorienses; Vol. 12 No. 1 (2008): Annales Bogorienses; 9-15 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7211/5455 Copyright (c) 2008 Maria Prihtamala Omega, Skye Thomas-Hall, Peer Schenk, Bostjan Kobe https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7212 2026-10-19T03:56:43Z annales:ART

Association between Polymorphism of β-Lactoglobulin Gene on Milk Yield and Quality in Local Sheep at Jonggol Animal Science Teaching and Research Unit (JASTRU) Sumantri, Cece Nurhayati, Nurhayati D. Farajallah, Ahmad Anggraeni, A. Anggraeni polymorphism b-lactoglobulin gene PCR-SSCP local sheep The aims of this study were to identify the effects of polymorphism of b-lactoglobulin gene on milk yield and percentage of milk protein and fat in local sheep. A total number of 83 heads of lactating ewes raised under an extensive management at Jonggol Animal Science Teaching and Research Unit (JASTRU) of the Faculty of Animal Science, Bogor Agricultural University were studied. Research activities were carried out through some steps involving blood collection. DNA isolation, DNA amplification, and separation of DNA fragments by electrophoresis with silver staining method. By using polymerase chain reaction (PCR) then genotyped by single strand conformation polymorphism technique (SSCP), it was successfully amplified a fragment length at 420 bp in b-lactoglobulin genelocated on the exon 7. The electrophoresis pattern revealed 5 types of b-lactoglobulin gene and designated as A, B, C, D, and E types. The proportion for those respective 5 type from the highest to the lowest were for A (27.71%), C (16.87%), D (12.05%), E (10.84%), and B (9.64%), respectively. There were no significant effect of polymorphisms in b-lactoglobulin gene on both milk yield and quality in local sheep at JASTRU. National Research and Innovation Agency (BRIN) 2008-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7212 10.14203/ann.bogor.2008.v12.n1.17-24 Annales Bogorienses; Vol. 12 No. 1 (2008): Annales Bogorienses; 17-24 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7212/5456 Copyright (c) 2008 Cece Sumantri, Nurhayati D. Nurhayati, Ahmad Farajallah, A. Anggraeni Anggraeni https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7230 2026-10-19T03:56:43Z annales:ART

Construction of an EPO (Human-Erythropoietin) Synthetic Gene Through a Recurvise-PCR Method Fuad, Asrul Muhamad Gusdinar, Tutus Retnoningrum, Debbie Sofie Natalia, Dessy human erythropoietin (hEPO) erythropoiesis EPO-synthetic gene recursive-PCR Pichia pastoris hematopoietic cytokine Human erythropoietin (hEPO) is an important glycoprotein in human that is coded by a single gene named EPO (erythropoietin). EPO is a glycoprotein hormone that promotes erythropoiesis, which is the formation process of mature red blood cell (erythr eyte ) in human bodies. It is widely used for treatment of anemia in patient with chronic renal failure. Therefore EPO has been classified as hematopoietic cytokine. Recombinant hEPO (rhEPO) has been commercially available, such a Epogen. It is produced in mammalian cell, such as CHO (Chinese hamster ovary) ceil for the reason of it· complex structure as a glyco-protein. In an effort to use and optimize heterologous EPO gene expression in an alternative eukaryotic host cells such as yeast, an EPO synthetic gene (EPOsyn) was constructed. The synthetic gene had been designed to contain optimal Pichia pastoris codon usage. It had been constructed by a recursive-PCR method in two-step PCR reactions. The gene was assembled from 8 single strands synthetic oligonucleotides having an average length of 90 nt with 20 to 30 overlap region between two adjacent oligos. The synthetic gene has less GC content (45.31%) compared its native (human) gene (59.08%). The synthetic gene has been cloned in pCR2.1 cloning plasmid and sequenced. From 8 independent clones, it was revealed that the error rate was 1.59%, in which 1.42% was due to deletions and 0.17% due to substitutions. Design of the gene sequences, construction method and DNA sequence analysis of the gene will be discussed in this paper. National Research and Innovation Agency (BRIN) 2008-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7230 10.14203/ann.bogor.2008.v12.n1.25-38 Annales Bogorienses; Vol. 12 No. 1 (2008): Annales Bogorienses; 25-38 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7230/5471 Copyright (c) 2008 Asrul Muhamad Fuad, Tutus Gusdinar, Debbie Sofie Retnoningrum, Dessy Natalia https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7231 2026-10-19T03:56:43Z annales:ART

In Vitro Propagation of Buah Merah (Pandanus Conoideus Lam) Through Lateral Bud Proliferation Imelda, Maria Wulansari, Aida Sumarnie, Sumarnie buah merah (Pandanus conoideus) lateral buds BAP TDZ KN Pandanus conoideus Lam or 'Buah merah' of the Pandanaceae is a native to East Indonesia, particularly Papua and North Maluku. Traditionally the fruits are used for health promotion and maintenance as well as for curing several illnesses. Recently, it has been reported that the fruit its are potential for cancer medication. As a result, there has been an overexploitation of the plants from their habitats. In order to anticipate their possible disappearance due to overexploitation in the wild, an efficient and effective technology for the mass propagation, conservation, and cultivation of these plant should be developed. Generally buah merah is propagated vegetatively by offshoots and stem cuttings or generatively by seeds. Micropropagation has many advantages over the conventional method because the technique allows mass clonal and pathogen-free production of plants at a high rate of multiplication all year round. In this research the effects of 0.1-0.2 mg/L thidiazuron (TDZ), 0.5-1.0 mg/L benzyl amino purine (BAP), and 0.25-0.5 mg/L kinetin (KN) on shoot bud induction and proliferation of P. conoideus were investigated using nodal infections or lateral buds of P. conoideus on modified Murashige and Skoog (MS) medium. Shoots were rooted on MS medium without plant growth regulator (PGR). The result showed that lateral bud of Pandanus start d to initiate growth after 4-7 days in culture. The best medium for shoot proliferation was MS containing either 0.5 mg/l BAP with 0.1 mg/l TDZ or 1 mg/l BAP with 0.5 mg/l KN giving a multiplication rate of 16.5 shootlets per shoot bud explant after 8 weeks. Rooting of shoots was successfully conducted on MS medium without PGR. Acclimatization of rooted plantlets was achieved on a mixed medium of cocopeat and soil (1:1). National Research and Innovation Agency (BRIN) 2008-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7231 10.14203/ann.bogor.2008.v12.n1.39-43 Annales Bogorienses; Vol. 12 No. 1 (2008): Annales Bogorienses; 39-47 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7231/5472 Copyright (c) 2008 Maria Imelda, Aida Wulansari, Sumarnie Sumarnie https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7233 2026-10-19T03:56:43Z annales:ART

Effect of Culture Conditions on Phytase Production by Aspergillus ficuum in Solid-state Fermentation Using Rice Bran as Substrate Kusharyoto, Wien Sari, Martha Ridwanuloh, Asep Muhamad phytase solid-state fermentation Aspergillus ficuum nutritional factors rice bran Phytic acid is an antinutritional factor that forms 1–2% of most of the seeds and their co-products representing more than 60% of their total phosphorus. Monogastric and agastric animals are unable to utilize phytate phosphorus either due to lack of or insufficient amount of phytate degrading enzymes. Phytases (myo-inositol hexakisphosphate-phosphohydrolase) are a special class of phosphatases that catalyze the hydrolysis of phytic acid in a stepwise manner to lower inositol phosphates, myo-inositol and inorganic phosphate. Phytases are found naturally in plants and microorganisms and a sizeable number of phytases have been purified and characterized from various fungi, yeasts, and bacteria. The present investigation involves studies on the effect of moisture content, pH value and different media ingredients such as carbon, nitrogen, and surfactants on the production of phytase by the fungus Aspergillus ficuum DSM 932 in solid-state fermentation (SSF) using rice bran as substrate. The production of phytase by SSF was favored, when the fungus was grown at a moisture content of 60% and pH 7.0, resulted in a phytase activity of 5.2 units/g dry substrate. There was a 20% increase in phytase yield in the presence of sucrose in SSF medium, while glucose and fructose were not effective in enhancing the phytase activity when used individually. Yeast extract was found to be a favorable nitrogen source for phytase production by SSF, which resulted in a 20% increase in phytase activity. There was no significant effect in increasing phytase production with the use of either soy peptone or tryptic soy as nitrogen source. Approximately 30% inhibition in phytase activity was shown in the presence of the surfactant Tween-80 or Triton X-100 in the SSF. By supplementing rice bran with sucrose and yeast extract, and performing the SSF in tray bioreactors, a phytase activity of 6.76 units/g dry substrate could be obtained. National Research and Innovation Agency (BRIN) 2009-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7233 10.14203/ann.bogor.2009.v13.n1.1-11 Annales Bogorienses; Vol. 13 No. 1 (2009): Annales Bogorienses; 1-11 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7233/5473 Copyright (c) 2009 Wien Kusharyoto, Martha Sari, Asep Muhamad Ridwanuloh https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7234 2026-10-19T03:56:43Z annales:ART

Agrobacterium-mediated Transformation of Banana Musa acuminata AA cv "“Mas Lampung” with hpt Gene Using Sterile Corm as Target Tissue Estiati, Amy Nena, Ade Witjaksono, Witjaksono Agrobacterium hpt gene transgenic banana Mas Lampung Musa acuminate corm slices PCR The protocol for Agrobacterium-mediated transformation of local banana plants cv “Mas Lampung” (AA) has been established. A selectable marker gene (hpt) has been used to study the transformation using in vitro corm slices as target tissues. Banana in vitro corm slices were co-cultivated with the EHA105 strain of Agrobacterium tumefaciens harbouring binary vector pCAMBIA 1301 containing hygromycin resistance gene (hpt) as a selectable marker and intron-containing β-Glucuronidase (gus-intron) gene as a reporter gene driven by CaMV 35S promoter. Polymerase Chain Reaction (PCR) were used to examine the existence of hpt gene in plants resulted from the transformation. Using primer pairs specific for hpt gene, our PCR analysis on leaves showed the presence of the hpt transgene in banana transgenic plants at first generation (T0) of transformation. To prove the existence of hpt gene in the fruits of transgenic banana plants, PCR analysis were also carried out. The data showed that the hpt gene could be amplified from banana fruits of tested samples. These result demonstrates that the Agrobacterium-mediated transformation method used in this experiment has been successful to transfer gene into banana plants. Thus, the transformation method reported here could be used as a standard protocol to transfer another useful genes into local banana plants cv. “Mas Lampung”. Furthermore, the presence of transgene in fruits of banana transgenic plants is important achievement especially for transgene that is expected to be expressed in the fruit including to introduce vaccine genes into banana fruits for edible vaccine.  National Research and Innovation Agency (BRIN) 2009-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7234 10.14203/ann.bogor.2009.v13.n1.12-20 Annales Bogorienses; Vol. 13 No. 1 (2009): Annales Bogorienses; 12-20 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7234/5475 Copyright (c) 2009 Amy Estiati, Ade Nena, Witjaksono Witjaksono https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7236 2026-10-19T03:56:43Z annales:ART

Propagation of Sukun (Artocarpus altilis (Parkinson) Fosberg) Through In Vitro Shoot Proliferation Imelda, Maria Wulansari, Aida Sari, Laela Sukun (Artocarpus altilis) in vitro shoot proliferation benzyl amino purine (BAP) adenine sulphate (AS) coconut water (CW) indole butyric acid (IBA) Sukun (Artocarpus altilis (Parkinson) Fosberg) of the Moraceaae is a big tree which can grow to 15-20 m in height and native to the Asia-Pasific region. Beside its delicious fruit, sukun is also known as a traditional herbal medicinal plant in the region, including Indonesia. Nearly all parts of the plant, such as roots, stems and leaves are believed by local communities to be capable of curing liver disease, hypertension, cardiac arrest, toothache, renal problem and even skin itchiness. The collaborative research between LIPI and PR China, on developing herbal medicines indicated that sukun has a great potential for treating cardiovascular disease. However, the availability of raw materials still poses a big constraint for the industry of herbal medicines. Generally, sukun is propagated by root or stem cuttings, since in Indonesia sukun does not produce any seeds. However such method only produces limited planting materials. In general tissue culture propagated plants have many advantages, namely being clonal, free from pest and diseases, more uniform, and allowing a high rate of plant multiplication. Therefore, the technique for sukun propagation has been developed by the LIPI Research Centre for Biotechnology. In this research the effects of 1-5 mg/l benzyl amino purine (BAP) and 20-40 mg/l adenine sulphate (AS)on shoot bud proliferation were investigated using lateral shoot buds on a modified Murashige and Skoog (MS) medium with addition of 150 ml/l coconut water (CW). Shoots were rooted on MS medium without plant growth regulators (PGRs). The results showed that the best medium for in vitro shoot proliferation was a modified MS medium containing 2 mg/l BAP, 40 mg/l AS and 150 ml/l CW. The best medium for rooting is MS medium containing 1 mg indole butyric acid (IBA), producing roots within 3 weeks.  National Research and Innovation Agency (BRIN) 2009-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7236 10.14203/ann.bogor.2009.v13.n1.21-28 Annales Bogorienses; Vol. 13 No. 1 (2009): Annales Bogorienses; 21-28 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7236/5476 Copyright (c) 2009 Maria Imelda, Aida Wulansari, Laela Sari https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7237 2026-10-19T03:56:44Z annales:ART

Identification of Drought Tolerant Related Insertional Mutant Lines Using PEG 6000 Nugroho, Satya Windiastri, Vincentia Esti Widyajayanti, Dwi Pantouw, Carla Frieda Oryza sativa insertion mutant drought PEG 6000 Degradation Index Vigor Index Drought is one of the most important abiotic stresses in rice (Oryza sativa) productivity. The development of drought tolerant cultivars are therefore highly desireable. We have developed insertional mutant based on the Japonica rice cv Nipponbare rice by transposons Ac/Ds insertions containing activation-tag and gene trap. Screening of the mutant population for drought tolerant related phenotypes is of our priority. The screening protocol based on PEG 6000 has been developed and was being used to screen 70 mutant lines tocharacterize their responds to the treatment based on different parameters (number of leaf, total weight, plant height, root length and number of germinating seeds). These characters were used to score the Degradation Index and Vigour Index. Results showed varyingresponds of the lines to the osmotic pressure. Some lines showing a good performance indicated by lower Degradation Index and higher Vigour Index have been identified. Some inconsistencies in the performances scored by both indices were thought to be due to seed quality. National Research and Innovation Agency (BRIN) 2009-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7237 10.14203/ann.bogor.2009.v13.n1.29-36 Annales Bogorienses; Vol. 13 No. 1 (2009): Annales Bogorienses; 29-36 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7237/5477 Copyright (c) 2009 Satya Nugroho, Vincentia Esti Windiastri, Dwi Widyajayanti, Carla Frieda Pantouw https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7239 2026-10-19T03:56:44Z annales:REV

Gene Flow from Genetically Modified Rice to Their Weedy and Wild Relatives and Its Environmental Consequences Estiati, Amy Nugroho, Satya Rice is one of the most important food crops in the world. Nearly half of the world population consume rice as their staple food (FAO, 2004). With the increasing of world population, the need to provide more food supplies including rice is obvious. Biotechnology is expected to play major roles in the improvement of crop productivity and quality. Unlike transgenic maize, eventhough many research have been done to improve rice performance by genetic engineering, transgenic rice has not been released or commercialized. Among those research to improve rice performance are the attempt to introduce biotic and abiotic stress tolerant traits, herbicide tolerant trait and beta-carotin biosynthetic pathway. Currently, several genetically modified rice cultivars have been and being tested on limited field trials. Undoubtedly, biotechnology will benefit agriculture and thus providing enough food source to keep up with the ever-increasing needs in the future. However, the release and utilization of such technology in agriculture is still arising concerns about the impact to the environment. Therefore the possibility of the transgene escape to the environment needs to be analyzed. For example, whether the application of such technology can create superweed that resulted in the environment problems. On the other hand, cultivated rice may acquire genes for weediness from pollen-mediated gene flow of weedy or wild rice occurring inside or near cultivated rice fields, which leads to persistence and invasiveness of the cultivated rice, although the chance is low. This article discuss the possibility of the occurrence gene flow from the application of genetically modified rice to surrounding rice plants including its wild relatives and weed, and the requirements or precautions needed to be done to prevent gene flow.  National Research and Innovation Agency (BRIN) 2009-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7239 10.14203/ann.bogor.2009.v13.n1.37-45 Annales Bogorienses; Vol. 13 No. 1 (2009): Annales Bogorienses; 37-45 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7239/5479 Copyright (c) 2009 Amy Estiati, Satya Nugroho https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7243 2026-10-19T03:56:44Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7243 10.14203/annalesbogorienses.2010.v14.n1.i Annales Bogorienses; Vol. 14 No. 1 (2010): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7243/5482 Copyright (c) 2010 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7244 2026-10-19T03:56:44Z annales:REV

Application of Microwave Heating in Biomass Hydrolysis and Pretreatment for Ethanol Production Hermiati, Euis Mangunwidjaja, Djumali Sunarti, Titi C. Suparno, Ono Prasetya, Bambang biomass pretreatment ethanol microwave Recently, due to depletion of fossil derived energy stock in the world, there are growing interests in utilizing biomass as sources of bioethanol. There are basically two types of biomass that are usually used or converted to ethanol, starchy and lignocellulosic biomass. The conversion of starchy and lignocellulosic materials are widely explored, however, there are still some drawbacks, such as high enzyme cost and intensive energy needed. Therefore, lower cost as well as energy and time efficient process technology in biomass conversion to ethanol is important in enhancing the use of biomass to substitute fossil fuel. Microwave heating offers some advantages to overcome these drawbacks, especially due to its quick heat transfer and its heating selectivity. In conventional heating the heat was transferred through conduction or convection process which took longer time. Thus, by using microwave, degradation of starchy and lignocellulosic biomass could be completed in shorter time than by using conventional heating method. The roles of microwave heating in the degradation of biomass, especially starchy and lignocellulosic biomass and its relation to the hydrolysis and pretreatment of that particular biomass for ethanol production are reviewed and discussed. National Research and Innovation Agency (BRIN) 2010-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7244 10.14203/ann.bogor.2010.v14.n1.1-9 Annales Bogorienses; Vol. 14 No. 1 (2010): Annales Bogorienses; 1-9 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7244/5483 Copyright (c) 2010 Euis Hermiati, Djumali Mangunwidjaja, Titi C. Sunarti, Ono Suparno, Bambang Prasetya https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7245 2026-10-19T03:56:44Z annales:ART

Control of Biodegradability of Polyurethane Foam Based on Palm Oil by Ratio of Soft Segment on the Polymer Backbone Haryono, Agus Triwulandari, Evi Sondari, Dewi Randy, Ahmad Ridwanuloh, Asep Muhamad biodegradable polyurethane palm oil polyols soft segment Polyurethane is polymer synthetic which is formed by reaction between polyols and polyisocyanates. Polyols are compounds which contain two or more hydroxyl groups. Polyurethane can be applied in various forms, such as foam, elastomeric, coating and adhesive. In this work, we developed polyurethane foams by using polyols based on palm oil. Palm oil based polyols as soft segments in the polyurethane backbone accelerate biodegradable process of the polyurethane. Biodegradability is the ability of material to be utilized as a carbon source by microorganisms and converted safely into carbon dioxide, biomass and water. Commercially available polyurethane foams are usually produced from petrochemical based polyols that they are not biodegradable. The biodegradability study was conducted by using Aspergillus niger (fungi) and Pseudomonas aeruginosa (bacteria), with variation of method and media for biodegradation. Rigid polyurethane foam was synthesized by using one shoot process method, which is reaction between palm-based polyol mixture and methylene diphenyl diisocyanate (MDI) at room temperature. The effect of variation of ratio of polyisocyanates (NCO) as hard segment and polyols (OH) as soft segment to the properties of polyurethane foams were studied in detail. Study of the effect NCO/OH ratio was conducted with variation ratio of 0.8, 1.0 and 1.2. Increasing hard segment (NCO) ratio to the soft segment (OH) affected in the decreasing of hydrophilicity of foam. Thus, the penetration of water into bulk of polymer decreased. The biodegradability of foam was also affected by the soft segment ratio on the polymer backbone. National Research and Innovation Agency (BRIN) 2010-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7245 10.14203/ann.bogor.2010.v14.n1.11-17 Annales Bogorienses; Vol. 14 No. 1 (2010): Annales Bogorienses; 11-17 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7245/5484 Copyright (c) 2010 Agus Haryono, Evi Triwulandari, Dewi Sondari, Ahmad Randy, Asep Muhamad Ridwanuloh https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7246 2026-10-19T03:56:44Z annales:ART

Screening for Natural Producers Capable of Producing 1,3-Propanediol from Glycerol Andriani, Dian Kusharyoto, Wien Prasetya, Bambang Willke, Thomas Vorlop, Klaus Dieter 1,3-propanediol enrichment culture glycerol palm oil screening Glycerol is a renewable resource found as the main by-product in the transesterification of triglycerides and fat saponification. Due to the increased production of plant oils, especially palm oil in developing countries, and their larger use by the oleochemical industry, glycerol surpluses are on the world market and this may result in a decrease in glycerol price. As a consequence, biotechnological processes have been developed to convert this substrate into value-added products, such as 1,3-propanediol (1,3-PD). The microbial production of 1,3-PD could be competitive to chemical routes assuming that it is based on cheap raw material and an optimised process. In the screening for 1,3 PD–producing bacteria, raw glycerol as by-product from rapeseed oil processing unit was used as a carbon source compared with commercial glycerol. By using increasing concentration of both glycerols from 50 to 150 g/l, two potential bacteria were obtained from soil samples. BMP-1 was obtained from an enrichment culture using 50 g/l commercial glycerol, while BMR-1 was obtained from an enrichment culture using 100 g/l raw glycerol. The highest conversion yield obtained using the isolate BMP-1 was around 0.62 g 1,3-PD formed per mol glycerol consumed, and 0.73 mol 1,3-PD formed per mol glycerol using the isolate BMR-1. No bacteria were obtained from cultures using 150 g/l commercial and raw glycerol, respectively, which indicated that higher concentration of glycerol has inhibition effect.  National Research and Innovation Agency (BRIN) 2010-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7246 10.14203/ann.bogor.2010.v14.n1.19-24 Annales Bogorienses; Vol. 14 No. 1 (2010): Annales Bogorienses; 19-24 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7246/5485 Copyright (c) 2010 Dian Andriani, Wien Kusharyoto, Bambang Prasetya, Thomas Willke, Klaus Dieter Vorlop https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7247 2026-10-19T03:56:44Z annales:ART

The Influence of Harvesting Period on Lipid Associated Antioxidant Activity of Semicontinuously Grown Chlorella vulgaris Chrismadha, Tjandra Sartika, Diani Setyaningsih, Iriani Uju, Uju microalgae Chlorella vulgaris lipid antioxidant harvesting period A green alga, Chlorella vulgaris, was grown semi-continuously at various harvesting periods, and the lipid content and its associated antioxidant activity was examined. The harvesting periods were 9, 18, and 27 days, while the algal culture was placed in 10 L glass bottles provided with aeration for gas exchange and mixing. Light source was obtained from 440 watt cool fluorescent lamps placed at one side of the culture. Room temperature was 26-32C. Algal lipid extraction was done based on liquid phase separation ofmethanol:chloroform:water and the antioxidant activity was examined by means of oxidation inhibitory in ethanol emulsified limnoleic acid. In addition, a TLC analysis was performed to identify the antioxidant compounds soluble in the lipid. The results showed that harvesting period had a significant influence on the algal lipid content, which were 11.94, 12.96, and 16.51% of the dry weight in the culture with harvesting periods of 9, 18, and 27 days, respectively. No remarkable effect of the harvesting period on the antioxidant activity, which were observed to inhibit oxidation of linoleic acid up to 67-71%. There were five compounds found can be associated with the algal antioxidant activity, which were pheophorbide-a, chlorophyll-b, chlorophyll-a, phaeophytin-a, -carotene, and an unidentified one.  National Research and Innovation Agency (BRIN) 2010-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7247 10.14203/ann.bogor.2010.v14.n1.25-30 Annales Bogorienses; Vol. 14 No. 1 (2010): Annales Bogorienses; 25-30 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7247/5486 Copyright (c) 2010 Tjandra Chrismadha, Diani Sartika, Iriani Setyaningsih, Uju Uju https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7248 2026-10-19T03:56:44Z annales:ART

Screening and Optimization of Cellulase Production of Bacillus subtilis TD6 Isolated from Takifugu rubripes Fish Andriani, Dian Park, Don Hee cellulase-producing bacteria Takifugu rubripes Bacillus subtilis carboxymethyl cellulose DNS method Cellulase enzymes have attracted considerable attention in recent years due to their great biotechnological and industrial potential. Cellulase enzymes provide a key opportunity for achieving tremendous benefits of biomass utilization through the bioconversion of the most abundant cellulosic material into the simplest carbohydrate monomer, glucose. Nowadays, the sources of cellulase-producing bacteria have been broadened into the presence of symbiotic bacteria in herbivorous animal and also from marine. Takifugu rubripes or known as Puffer fish is a unique poisonous vertebrate but nevertheless is considered a delicacy in Korea. The diet of the puffer fish includes mostly algae. This dietary habit considers Puffer fish as host of cellulase-producing bacteria, especially on its gut. In the present study an attempt has been made to search for the cellulolytic bacteria in the gut of Takifugu rubripes. Fifty five microorganisms have been isolated using 1% (w/v) carboxymethyl cellulose (CMC) as substrate. Congo red dye test and DNS method were then used for screening the extracellular cellulase activity of the strains. Among them, TD6 strain has shown the highest performance in term of cellulase activity. In order to evaluate the optimum culture condition of the isolate TD6 for cellulase production, the strain was grown at various temperatures, pH, carbon sources, and nitrogen sources. Under optimum condition, the maximum specific activity of 2.13 U/mg protein was achieved after growth the strain with 1.5% CMC at 45ºC pH 6 for 3 days, respectively. Based on 16S rRNA gene analysis it is proposed that the strain was identified as Bacillus subtilis.  National Research and Innovation Agency (BRIN) 2010-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7248 10.14203/ann.bogor.2010.v14.n1.31-37 Annales Bogorienses; Vol. 14 No. 1 (2010): Annales Bogorienses; 31-37 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7248/5487 Copyright (c) 2010 Dian Andriani, Don Hee Park https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7249 2026-10-19T03:56:44Z annales:ART

Rapid Assessment of Diverse Trichodermal Isolates of Indonesian Origin for Cellulase Production Fahrurrozi, Fahrurrozi Ratnakomala, Shanti Lisdiyanti, Puspita Anindyawati, Trisanti Sukara, Endang Trichoderma cellulase Culture Collection Trichoderma is a well-known candidate to be promoted as cellulase producer for the hidrolysis of lignocellulose that contain in woody biomass. The number of trichodermal isolates in our laboratory collected from diverse ecosystem types in Indonesia increases significantly during the last 5 years. It is our aim to assess the cultures for its ability in producing cellulase. Sixty-six trichodermal isolates used in this experiment are obtained from Biotechnology Culture Collection (BTCC), Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI). The 31 isolates were isolated from District of Liwa (South Sumatra, Indonesia) and the 35 isolates from District of Maros (South Sulawesi, Indonesia). The isolates were screened qualitatively, 7 isolates from Liwa and 12 isolates from Maros showed cellulolytic activity. From the results of quantitative test, two strains (ID08-T004 and ID08-T63) showed the higher cellulolytic activity among the selected strains, 133.5 and 133.5 U/ml, respectively. These extracellular enzymes were characterized their temperature and pH optimum. The temperature optimum for both enzymes was the same, 50C, with activity 213.6 U/ml for enzyme extracted from ID08-T004 and 197.3 U/ml for enzyme from ID08-T0063. The pH optimum was pH 5 of ID08-T004 with activity 137.7 U/ml and pH 6 for ID08-T063 with activity 75.0 U/ml. The enzymes from ID08-T004 and ID08-T063 were stable in their temperature and pH optimal condition even after 90 minutes incubation with activity 179.0 U/ml and 86.7 U/ml, respectively. The enzyme stability was approximately 150 minutes for both enzymes in the temperature and pH optimum.  National Research and Innovation Agency (BRIN) 2010-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7249 10.14203/ann.bogor.2010.v14.n1.39-46 Annales Bogorienses; Vol. 14 No. 1 (2010): Annales Bogorienses; 39-46 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7249/5488 Copyright (c) 2010 Fahrurrozi Fahrurrozi, Shanti Ratnakomala, Puspita Lisdiyanti, Trisanti Anindyawati, Endang Sukara https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7250 2026-10-19T03:56:44Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7250 10.14203/annalesbogorienses.2010.v14.n2.i Annales Bogorienses; Vol. 14 No. 2 (2010): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7250/5489 Copyright (c) 2010 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7251 2026-10-19T03:56:44Z annales:ART

Conservation of Major L1 and Variability of Minor L2 Capsid Late Protein Genes in Human Papillomavirus of Indonesia Variants Nuswantara, Sukma Prana, Titik K. Wulandari, Dwi Widyowati, Henni Anzela, Vera Levy, Dea Cahyadi, Petrus Tjandra, Lukas D Human Papilloma Virus (HPV) L1 gene L2 gene cervical cancer high risk HPV Human Papilloma Virus (HPV) has an outstanding feature for its vast intraspecies variability. Of all known 100 types or more, 15 types of them are classified as high risk because of their occurrence in more than 95% of cervical cancer cases. Among all genes in their genome, E6 and E7 genes are considered oncogenes and have close relevance with their pathogenicity, whilst L1 and L2 genes produce capsid proteins that directly interact with their host receptors. Considering the importance of L1 and L2 in host-receptor relationship, we tried to investigate their molecular variability thereby uncover their specificity as Indonesian variants. Here we reported about the conservation of L1 minor capsid protein and variability of L2 capsid protein among high-risk types Human Papilloma Virus (HPV). The results indicated that L1 DNA was relatively more conserved than its L2 counterpart. Also it was indicated that the middle part of either L1 or L2 CDS‟ showed more DNA variability than those at their upstream sequences. It is concluded that L2 middle sequences are important factors for intraspecific variations found in HPV of Indonesian variants. National Research and Innovation Agency (BRIN) 2010-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7251 10.14203/ann.bogor.2010.v14.n2.1-8 Annales Bogorienses; Vol. 14 No. 2 (2010): Annales Bogorienses; 1-8 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7251/5511 Copyright (c) 2010 Sukma Nuswantara, Titik K. Prana, Dwi Wulandari, Henni Widyowati, Vera Anzela, Dea Levy, Petrus Cahyadi, Lukas D Tjandra https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7278 2026-10-19T03:56:44Z annales:ART

The Development of A Bioassay Based on Heterologous Expression of M2 Ion-Channel Protein Prasetyoputri, Anggia Yuliaty, Neti Tuharea, Warda Febyanti, Alisin Sunarko, Bambang Atmosukarto, Ines I. C. influenza M2 bioassay gene synthesis antiviral Emerging resistant viral strains combined with the limited availability of antivirals in a pandemic scenario highlight the need for the development of novel influenza antivirals. A bioassay based on the M2 protein of influenza virus - a potential target for antivirals - was developed to screen endophytic microbial extracts. M2 can be synthesized using PCR, thus eliminating the need for the handling of infectious specimen. Following cloning of the M2 gene into a pET backbone, the resultant plasmid was transformed into BL21 (DE3) pLyss E. coli cells. Cultures of these cells were set up at 37C following inoculation with a starter culture, to reach an OD at 600nm (OD600) of 0.4-0.6. Once at the required OD, the culture was split in two aliquots and expression of the M2 protein was induced in one of the duplicates with the addition of isopropyl β-D-thiogalactopyranoside (IPTG). Bacterial growth was monitored at 60-minute intervals. Exogenous expression of the M2 protein has been reported to decrease host cells viability, resulting in lower OD600 values. Our results suggest that the M2 protein was expressed and that overexpression of this protein resulted in consistently lower OD600 values of induced cultures compared with that of uninduced cultures. Based on this principle, extracts can be screened for their ability to block M2 function as identified by increased OD600 values.  National Research and Innovation Agency (BRIN) 2010-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7278 10.14203/ann.bogor.2010.v14.n2.9-14 Annales Bogorienses; Vol. 14 No. 2 (2010): Annales Bogorienses; 9-14 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7278/5512 Copyright (c) 2010 Anggia Prasetyoputri, Neti Yuliaty, Warda Tuharea, Alisin Febyanti, Bambang Sunarko, Ines I. C. Atmosukarto https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7280 2026-10-19T03:56:44Z annales:ART

Construction of pY-Af Vector for Expression of Thermostable α-L-Arabinofuranosidase in Saccharomyces cerevisiae Wirajana, I Nengah Puspaningsih, Ni Nyoman Tri Wasito, Eddy Bagus Kusuma, Soekry Erfan Kimura, Tetsuya Sakka, Kazuo α-L-arabinofuranosidase Saccharomyces cerevisiae expression vector In this research, construction of expression vector for thermostable α-L-arabinofuranosidase in Saccharomyces cerevisiae BJ1824 was conducted. The Escherichia coli/S. cerevisiae shuttle vector, pYES2 was used as parental vector in construction. The abfA gene encoding α-L-arabinofuranosidase from Geobacillus thermoleovorans IT-08 was amplified by PCR, in which the plasmid pTP510 was used as a template. The amplification product was treated with SacI and XhoI and then subcloned to the pYES2 vector, which was previously digested with SacI and XhoI. The recombinant plasmid was designated as pY-Af and propagated first in E. coli Top10, and then transformed into S. cerevisiae BJ1824. For α-Larabinofuranosidase (AbfA) production, the yeast transformants were grown in YNBG selective medium and YPG rich medium, using galactose as an inducer. The AbfA activity was assayed by measuring the amount of p-nitrophenol (pNP) released from p-nitrophenyl-α-L-arabinofuranoside (pNPA) substrate at pH 6.0 and 70oC for 30 min. The recombinant AbfA activity was detected in either of culture medium (0.98%), cellassociated (14.17%) and intracellular (84.85%) when recombinant yeast was grown in YPG rich medium. National Research and Innovation Agency (BRIN) 2010-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7280 10.14203/ann.bogor.2010.v14.n2.15-20 Annales Bogorienses; Vol. 14 No. 2 (2010): Annales Bogorienses; 15-20 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7280/5515 Copyright (c) 2010 I Nengah Wirajana, Ni Nyoman Tri Puspaningsih, Eddy Bagus Wasito, Soekry Erfan Kusuma, Tetsuya Kimura, Kazuo Sakka https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7282 2026-10-19T03:56:44Z annales:ART

Improvement of Genetic Transformation Efficiency in Vanda tricolor Orchid Using Acetosyringone Dwiyani, Rindang Purwantoro, Azis Indrianto, Ari Semiarti, Endang Vanda tricolor Agrobacterium orchid protocorms acetosyringone Vanda tricolor Lindl. var. suavis is an Indonesian wild orchid which is now extremely rare in nature due to its habitat destruction. Development of an appropriate method for improving Vanda orchid through genetic modification could be valuable for horticulture and, indirectly, also for conservation. In this research, a method of Agrobacterium-mediated transformation of two V. tricolor obtained from Salak Mount, West Java and Merapi Mount, Yogyakarta in Indonesia protocorms was improved using acetosyringone (AS). Concentrations of 0 and 25 ppm AS were used in transformation of pG35S binary vector containing kanamycin resistance geneinto V. tricolor protocorms. The result showed that 25 ppm AS was required on inoculation with Agrobacterium solution, without AS on cocultivation. Five weeks after treatment on the 300 ppm kanamicyn containing medium, green protocorms were obtained, that was 11.01% for V. tricolor from Salak Mount with pre-culture treatment prior to inoculation, 9.39% for V. tricolor from Merapi Mount with pre-culture treatment prior to inoculation, and 1.37% for V. tricolor from Merapi Mount without pre-culture treatment prior to inoculation. The best condition to set high efficiency of transformation is pre-culture protocorms prior inoculation, soaking protocorm on 25 ppm AS for 30 minutes, then co-cultivate its on AS-free callus induction medium. National Research and Innovation Agency (BRIN) 2010-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7282 10.14203/ann.bogor.2010.v14.n2.27-32 Annales Bogorienses; Vol. 14 No. 2 (2010): Annales Bogorienses; 27-32 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7282/5517 Copyright (c) 2010 Rindang Dwiyani, Azis Purwantoro, Ari Indrianto, Endang Semiarti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7285 2026-10-19T03:56:44Z annales:ART

Site-Directed Mutagenesis of Glu-269 L-Arabinose Isomerase from Geobacillus stearothermophilus Isolated from Tanjung Api Poso, Indonesia Fitriani, Dewi Wulandari, Puspita Suci Saksono, Budi site-directed mutagenesis Q269K L-arabinose isomerase Geobacillus stearothermophilus Industrializing of tagatose requires enzymes that meet to industrial need such as thermophile, slightly acidic and metal independent. Previously, we cloned, sequenced and expressed L-arabinose isomerase from Geobacillus stearothermophilus isolated from Tanjung Api, Poso, Indonesia. Based on DNA alignment analysis, the gene had high homology with those of G. stearothermophilus T6 (Gene Bank Acc No: AAD45718) which has optimum activity at high temperature and alkaline condition. In this paper, we described site-directedmutagenesis approach to mutate Glu-269 (Q269) to Lys-269 (K269) to decrease the optimum pH of the strain. Sequencing result showed that mutagenesis had been successful to mutate amino acid at position 269 from glutamine (Q) into lysine (K). Expression of mutant Q269 showed protein with molecular mass ~56 kDa.  National Research and Innovation Agency (BRIN) 2010-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7285 10.14203/ann.bogor.2010.v14.n2.33-37 Annales Bogorienses; Vol. 14 No. 2 (2010): Annales Bogorienses; 33-37 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7285/5519 Copyright (c) 2010 Dewi Fitriani, Puspita Suci Wulandari, Budi Saksono https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7288 2026-10-19T03:56:44Z annales:Pre

EDITOR's PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7288 10.14203/annalesbogorienses.2011.v15.n1.i Annales Bogorienses; Vol. 15 No. 1 (2011): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7288/5521 Copyright (c) 2011 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7289 2026-10-19T03:56:44Z annales:ART

Protective Effect of CD4+CD25+ Regulatory T Cells on Mice Model of Rheumatoid Arthritis Rifa'i, Muhaimin CD4+CD25+ Regulatory T cells Rheumatoid Arthritis Andrographis paniculata autoimmune Naturally occurring CD4+CD25+ regulatory T (Treg) cells, a component of the innate immune response which play a key role in the maintenance of self-tolerance, have become the focus of numerous studies over the last decade. These cells have the potential to be exploited to treat autoimmune disease. These cells inhibit the immune response in an antigen-nonspecific manner by interacting with other T cells. These T cell populations actively control the properties of other immune cells by suppressing their functional activity to prevent autoimmunity but also influence the immune response to allergens as well as against tumor cells and pathogens. In this experiment we showed that induced regulatory T cells have a protective effect on mice model of rheumatoid arthritis (RA). RA mice which were injected intraperitoneally with Andrographis paniculata substrate or injected with induced regulatory T cells showed the effects of recovery. We further showed that the generation of leukocyte including B cells can be promoted by the administration of A. paniculata substrate. Tissue damage from free radicals that arise due to imperfect metabolism can be prevented by such treatment in RA model mice. Recovery effects occurred in RA model mice involves the increasing number of CD4+CD25+ regulatory T cells. National Research and Innovation Agency (BRIN) 2011-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7289 10.14203/ann.bogor.2011.v15.n1.1-6 Annales Bogorienses; Vol. 15 No. 1 (2011): Annales Bogorienses; 1-6 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7289/5534 Copyright (c) 2011 Muhaimin Rifa'i https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7301 2026-10-19T03:56:44Z annales:ART

In vitro Growth Response of Taraxacum officinale Weber ex F.H. Wigg Regenerated from Different Type of Explants Ermayanti, Tri Muji Martin, Andri Fadillah Taraxacum officinale Weber ex F.H. Wigg growth response plant growth regulators different explants regeneration Dandelion (Taraxacum officinale Weber ex F.H. Wigg) is a medicinal plant species, reported to have some active compounds useful as anti-cancer, anti-inflammatory, antioxidants, and diuretics. Study on in vitro secondary metabolic production and tissue culture of these plants has been reported. This research was aimed to investigate plant regeneration from leaf blade, petiole and root as explants grown in selected media. Three different type of explants were cultured in MS solid medium supplemented with BAP (0, 0.5, and 1.0 mg/l) combined with NAA (0, 0.5, 1.0, and 2.0 mg/l) to select the best medium for spontaneous regeneration. Explants were grown for 6 weeks, the viability of explants, and formation of callus, shoots as well as roots were recorded. The results showed BAP at 1 mg/l combined with at 0.5 mg/l of NAA was suitable for shoot formation, whereas NAA alone was suitable for root formation from leaf blade and petiole. Root was the best explant for shoot regeneration, callus was grown at the first two weeks, and multiple shoots were grown after 3 weeks. No roots were found from root explant. The best medium for multiple shoot regeneration was MS containing 1 mg/l BAP without or with addition of 0.5 mg/l NAA. National Research and Innovation Agency (BRIN) 2011-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7301 10.14203/ann.bogor.2011.v15.n1.7-13 Annales Bogorienses; Vol. 15 No. 1 (2011): Annales Bogorienses; 7-13 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7301/5536 Copyright (c) 2011 Tri Muji Ermayanti, Andri Fadillah Martin https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7307 2026-10-19T03:56:44Z annales:ART

Comparison of the Reduction Effect of Sucrose and Table Sugar Concentration on Growth Characteristics of Red Ginger (Zingiber officinale Rocs.) Cultured in Liquid Medium Hapsari, Betalini Widhi Ermayanti, Tri Muji Rantau, Deritha E. Rudiyanto, Rudiyanto red ginger (Zingiber officinale Rocs.) reduction of sugar survival rate chlorophyll stomata The aim of this research was to compare the reduction effect of sucrose or table sugar concentration on growth characteristics of red ginger cultured in MS liquid medium. Shoots of red ginger was cultured on MS liquid medium without addition of plant growth regulators, supplemented with 5, 10, and 20 g/l of sucrose or table sugar for 8 weeks. Resulted plantlets were acclimatized in a greenhouse to investigate their growth and survival rate. Numbers of stomata, chlorophyll concentration as well as cross section of leaves from plantlets grown in vitro were compared to those of transplants grown in the greenhouse. The results showed that the use of table sugar at concentration of 20 g/l gave the best growth of red ginger. Meanwhile, the reduction of table sugar from 20 to 10 g/l reduced growth and survival rate of in vitro shoots as well as that of transplants in the greenhouse. Only few shoots formed roots when they were grown on the medium containing 5 g/l of table sugar, and transplants failed to grow in the greenhouse. It found that the chlorophyll content of in vitro plantlets was lower than those of transplants grown in the glasshouse. However, the number of stomata of the in vitro plantlets was higher than that of transplants grown in the glasshouse. There was no anatomical abnormalities found on the cross section of leaves between in vitro plantlets and transplants grown in the greenhouse. The replacement of sucrose with table sugar may reduce the production cost of plantlets.  National Research and Innovation Agency (BRIN) 2011-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7307 10.14203/ann.bogor.2011.v15.n1.15-20 Annales Bogorienses; Vol. 15 No. 1 (2011): Annales Bogorienses; 15-20 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7307/5540 Copyright (c) 2011 Betalini Widhi Hapsari, Tri Muji Ermayanti, Deritha E. Rantau, Rudiyanto Rudiyanto https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7308 2026-10-19T03:56:44Z annales:ART

Phenotypic Screening of Ds Transposon and Activation-tag Insertional Mutant Rice Population for Drought and Salinity Tolerant Related Traits Nugroho, Satya Zannati, Anky Situmorang, Apriadi Windiastri, Vincentia Esti Widyajayanti, Dwi Pantouw, Carla Frieda Astuti, Dwi Indrayani, Sri Rachmawati, Syamsidah Mulyaningsih, Enung Sri Santoso, Tri Joko Trijatmiko, Kurniawan Rudi rice insertion mutant gene discovery drought salinity The studies of rice genes through functional genomics are greatly facilitated by the availability of the complete genome sequences, including the complete physical map of the japonica rice cv. nipponbare. Using the Ac and Ds transposon, that are capable of transposition in various heterologous plants including the monocotyledon rice, combined with the enhancer element for generating activation taged lines, it is possible to discover and isolate functional genes involved in various important agronomical traits; such as those involved in abiotic stress tolerance (drought, high salt) and biotic stresses diseases and pests. We have developed 1,785 first (T0) generation of mutant nipponbare rice by transposons Ac/Ds insertions containing activation-tag, generated by transformation using Agrobacterium-mediated method. Currently, we have generated approximately 1,000 stable lines with transposon Ds and activation-tag insertion ready for screening. Efficient screening methods for mutant Nipponbare rice lines have been established for agronomically important mutant traits. Among the new phenotypes related to important agronomical traits observed were drought and salt tolerant or sensitive, stunted and robust growth, variable root penetration and other interesting traits such as reduced tillering, rolled leaf and thin tiller.  National Research and Innovation Agency (BRIN) 2011-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7308 10.14203/ann.bogor.2011.v15.n1.21-25 Annales Bogorienses; Vol. 15 No. 1 (2011): Annales Bogorienses; 21-25 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7308/5541 Copyright (c) 2011 Satya Nugroho, Anky Zannati, Apriadi Situmorang, Vincentia Esti Windiastri, Dwi Widyajayanti, Carla Frieda Pantouw, Dwi Astuti, Sri Indrayani, Syamsidah Rachmawati, Enung Sri Mulyaningsih, Tri Joko Santoso, Kurniawan Rudi Trijatmiko https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7309 2026-10-19T03:56:44Z annales:ART

Genetic Transformation of Rice cv. Ciherang Using Double T-DNA Vector Harboring cry1Ab Gene Sulistyowati, Yuli Rachmat, Agus Zahra, Fatimah Rachmawati, Syamsidah Nugroho, Satya double T-DNA transgenic rice Cry1Ab transformation Rice stem borer (Scirpophaga sp.) is considered as one of the primary pests causing yield decrease in Indonesia. Genetic engineering is considered as one of the most effective way to improve the resistance of rice plants to yellow stem borer since no resistance gene has been found in rice and its wild relatives. A double T-DNA binary vector p2TDNAcryIAb wich carry two independent T-DNA was used to transform Indonesian elite rice cv Ciherang using Agrobacterium-mediated transformation to generate transgenic rice that free from selectable marker genes. One T-DNA containing a selectable marker hygromycin phosphotransferase (hpt) gene and the other carrying the cry1Ab gene. As many as 37 putative transgenic plants from 14 embryos were generated. PCR analysis demonstrated that 22 out of 37 putative transgenic rice contain cry1Ab gene. All Ciherang rice carrying the cry1Ab gene expressing Cry1Ab protein as shown by the immunostrip assay. Further work will be carried out to determine the transgenes copy number, to select marker free transgenic rice, and to evaluate the resistance of transgenic rice against neonates larvae of yellow stem borer.  National Research and Innovation Agency (BRIN) 2011-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7309 10.14203/ann.bogor.2011.v15.n1.27-32 Annales Bogorienses; Vol. 15 No. 1 (2011): Annales Bogorienses; 27-32 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7309/5544 Copyright (c) 2011 Yuli Sulistyowati, Agus Rachmat, Fatimah Zahra, Syamsidah Rachmawati, Satya Nugroho https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7321 2026-10-19T03:56:44Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7321 10.14203/annalesbogorienses.2011.v15.n2.i Annales Bogorienses; Vol. 15 No. 2 (2011): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7321/5551 Copyright (c) 2011 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7322 2026-10-19T03:56:44Z annales:ART

Detection of Potyvirus Using RT-PCR and ACP-ELISA of Dioscorea Species and In Vitro Shoot Multiplication of the Virus Free Plants Wulandari, Dyah Retno Ermayanti, Tri Muji Dioscorea spp. Potyvirus RT-PCR ACP-ELISA in vitro shoot multiplication Detection of Potyvirus using Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Antigen Coated Plate-Enzyme Linked Immunoabsorbent Assay (ACP-ELISA) for Dioscorea alata, Dioscorea hispida, and Dioscorea esculenta was conducted in order to establish in vitro culture of virus-free of these species. Plants were collected from Yogyakarta, Lampung, Pasuruan, Jakarta and Bogor. Total RNA of plants grown in a greenhouse was then isolated according to Simple Direct Tube (SDT) method. Total RNA from symptomatic leaf of Yard Long Bean (Vigna unguiculata) infected with Bean Common Mosaic Potyvirus (BCMV) was used as the positive control treatment. RT-PCR assay with degenerate primers MJ1(F) and MJ2(R) was used to identify the Potyviruses infecting Dioscorea. ACP-ELISA with antibodies specific to group Potyvirus was carried out to detect Potyvirus from leaves samples. The Dioscorea virus-free species was then cultured on modified MS medium. Shoot tips or internodes were used as explants. The results showed that using both RT-PCR and ACP-ELISA, all species tested were free from virus. The growth response of explants on MS medium was varied depending on the plant species and the concentration of BAP.  National Research and Innovation Agency (BRIN) 2011-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7322 10.14203/ann.bogor.2011.v15.n2.1-8 Annales Bogorienses; Vol. 15 No. 2 (2011): Annales Bogorienses; 1-8 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7322/5552 Copyright (c) 2011 Dyah Retno Wulandari, Tri Muji Ermayanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7323 2026-10-19T03:56:44Z annales:ART

Micropropagation of Tetramolopium klossii (Moore) Mattfeld, Endemic Subalpine and Alpine Plant Species to New Guinea Ermayanti, Tri Muji Hafiizh, Erwin Al Martin, Andri Fadillah Mandessy, Ary Hermono, Arief Mukhsia, Andi Tetramolopium klossii micropropagation Murashige and Skoog medium shoot multiplication The protocol of micropropagation of Tetramolopium klossii (Moore) Mattfeld through adventitious shoot proliferation was established. This is the first report of T. klossii tissue culture. Seeds were surface-sterilized by dipping them in 10% of ethanol for 1 min followed by soaking in 1% of sodium hypochlorite for 10 min. The sterile seeds were germinated on MS medium solidified with 8 g/l of agar, supplemented with 20 g/l of sucrose. Two-month old seedlings formed some shoot clumps on MS solid medium without addition of any plant growth regulators. Small clumps containing 4-6 adventitious shoots were used for shoot proliferation on MS solid medium containing 0; 0.1; 0.5; 1.0 and 2.0 mg/l of BAP (benzylaminopurine) or kinetin. The results showed that medium MS without cytokinins (control treatment), and the addition of 0.1 or 0.5 mg/l of BAP was more effective for shoot proliferation compared to the medium with addition of BAP at higher concentration and the addition of kinetin. In further subcultures, multiple shoots grown on MS medium without cytokinins was more vigorous than that of shoots grown on other media, so that the shoots were ready for rooting. Medium containing 0.5 mg/l of BAP was the best for maintaining multiple shoot formation. Shoots formed roots on MS medium containing half strength of macro nutrients.  National Research and Innovation Agency (BRIN) 2011-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7323 10.14203/ann.bogor.2011.v15.n2.9-15 Annales Bogorienses; Vol. 15 No. 2 (2011): Annales Bogorienses; 9-15 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7323/5553 Copyright (c) 2011 Tri Muji Ermayanti, Erwin Al Hafiizh, Andri Fadillah Martin, Ary Mandessy, Arief Hermono, Andi Mukhsia https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7341 2026-10-19T03:56:44Z annales:ART

The Investigation of Polycyclic Aromatic Hydrocarbon and Oil Degrading Bacteria Isolated from The Marina Port Ancol, Jakarta Bay Lisdiyanti, Puspita Yopi, Yopi Murniasih, Tutik polycyclic aromatic hydrocarbons crude oil degrading bacteria bioremediation Polycyclic Aromatic Hydrocarbons (PAHs) as well as crude oil are widespread environmental pollutants. The contamination of air, soil, freshwater (surface water and groundwater), and marine environments by PAHs as well as crude oil has been reported. Of concern to public health is the fact that many PAHs or their metabolites are mutagenic, carcinogenic, or both. North Java coastal area such as Jakarta Bay is the polluted marine area in Indonesia as a result from anthropogenic wastes and the oil spill. Although evaporation and photo-oxidation play an important role in oil detoxification, ultimate and complete degradation is accomplished mainly by marine micro flora, and being dominant in this function. Certain bacteria are well-known could consume and degrade the PAHs as well as crude oil. Therefore investigating the potential PAH and oil degrading marine bacteria is important. In this study, we collected sample from oil polluted area in Marina Port Ancol, Jakarta Bay and isolated four PAH substrates and Arabian crude oil degrading marine bacteria using enrichment method and direct isolation method. As result, 223 strains could degrade PAHs, among these strains, 94 strains could degrade phenanthrene, 23 strains degrade fluoranthene, 92 strains could degrade dibenzotiophen, 14 strains could degrade phenotiazin and 106 isolates degrade crude oil.  National Research and Innovation Agency (BRIN) 2011-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7341 10.14203/ann.bogor.2011.v15.n2.17-23 Annales Bogorienses; Vol. 15 No. 2 (2011): Annales Bogorienses; 17-23 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7341/5568 Copyright (c) 2011 Puspita Lisdiyanti, Yopi Yopi, Tutik Murniasih https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7342 2026-10-19T03:56:44Z annales:ART

Alkane Degradation and Detection of Mono-xygenase Gene from Alcanivorax sp. from Jakarta Bay Thontowi, Ahmad Yopi, Yopi biodegradation alkane monooxygenase cloning alkB Alkanes is a major component of crude oil that can be hydrolized by enzyme alkane monooxygenase from bacteria. Nine oil-degrading bacteria were analyzed their capability to degrade alkanes (pristane and paraffin). The result of growth test on paraffin and pristane were showed that 9 isolates could be divided into two groups. First group (BL09, BL31 and BL45) could degrade both paraffin and pristane, and second group (BL01, BL06, BL44, BL057, BL058 and BL071) preferred to degrade paraffin than pristane. Three isolates (BL09, BL31 and BL45) have activity to decrease paraffin and pristane until less 50% remain. Based on homology analysis of 16SrRNA gene sequences showed that isolates No. BL09, BL31 and BL45 were identified as Alcanivorax sp. and the partial sequences of the alkB gene from those three isolates are showing 66-68% of identity compare with some mono-oxygenase gen from database of genbank.  National Research and Innovation Agency (BRIN) 2011-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7342 10.14203/ann.bogor.2011.v15.n2.25-30 Annales Bogorienses; Vol. 15 No. 2 (2011): Annales Bogorienses; 25-30 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7342/5569 Copyright (c) 2011 Ahmad Thontowi, Yopi Yopi https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7343 2026-10-19T03:56:44Z annales:ART

Ecological Study of Rare-Actinomycetes in Soils and Leaf-Litters Lisdiyanti, Puspita Ratnakomala, Shanti Ridwan, Roni Widyastuti, Yantyati Otoguro, Misa Ando, Katsuhiko Actinomycetes microbial diversity A total of 268 strains of Actinomycetes were isolated from soil and leaf-litter samples collected in Jambi (Sumatera) and Cibinong (West Java) using three selective isolation methods. All the isolates were identified by morphological characteristic and by analysis of 16S rDNA sequence. On the basis of their morphology and 16S rDNA sequence, 164 isolates were belonged to the Streptomyces Group and 104 isolates were belonged to the Rare-Actinomycetes (Non-Streptomyces) Group. Furthermore, 40% of isolates in Streptomyces Group and 62% of isolates in Non-Streptomyces Group are supposed to be new taxa. It is indicated the richness of Actinomycetes in these two area. The genus Streptomyces is the most abundant in soil samples, occupying 60-75% of all isolates; while the genus Actinoplanes is mainly found in leaf-litter samples (70%). The use of selective isolation media is important to elucidate the microbial diversity.  National Research and Innovation Agency (BRIN) 2011-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7343 10.14203/ann.bogor.2011.v15.n2.31-36 Annales Bogorienses; Vol. 15 No. 2 (2011): Annales Bogorienses; 31-36 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7343/5570 Copyright (c) 2011 Puspita Lisdiyanti, Shanti Ratnakomala, Roni Ridwan, Yantyati Widyastuti, Misa Otoguro, Katsuhiko Ando https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7344 2026-10-19T03:56:44Z annales:REV

Current Status of Sexing Bovine Spermatozoa In Indonesia Tappa, Baharuddin sperm sexing bovine BSA column Artificial Insemination The government of Indonesia has launched a Program Self-Sufficiency for Beef and Buffalo in Year 2014 (PSDS/K-2014). The objectives of this program include increasing population, improving productivity and increasing beef production, and increasing farmer income and welfare. To achieve the increasing population, the availability of elite cows is required. Currently, the main problem of development cattle in Indonesia is limited number of elite cows in terms of both quantitative and qualitative. One thing that needs to be done to achieve the program is an increase in the genetic quality and capacity through improved livestock production and productivity of livestock including use sexing sperm for artificial insemination (AI). The main application of sexed sperm to date has been to breed dairy heifers to produce female calves. To have calves with the sex according to the expectation, for example, female calve, we can perform the separation of sperm carrying the X chromosome so that the fertilized egg finally will become a female calve. Several methods have been performed to obtain optimal results and decent AI. Research Center for Biotechnology LIPI since 1999 until now, generate 70-90% of sperm Y. The results obtained by concentration of 5% and 10% is the ratio of the concentration of sperm that optimum separation column can be separated by 70-88% X sperm and Y sperm by 70-90%. Sperm motility before and after separation (sexing) is not too early for motility decreased from 76-80% to 67-80%. Difference increasing concentrations of BSA is expected to separate the spermatozoa that have a high motility (spermatozoa Y) will be able to penetrate the concentration of the more dense medium separation, whereas the X sperm will remain in the media that has a lower concentration. Generally indicates that, the separation of sperm quality after freezing is still fit for use for AI is characterized by sperm motility is still around 40-50%. After calves birth, offspring from sexed spermatozoa appear to have no more abnormalities than control spermatozoa. No significant effect on male calf and female calf at birth and weaning. Therefore, there appear to be no detrimental effect of sorting spermatozoa on resulting spermatozoa. Successful use of sexed sperm requires excellent management of cattle, careful handling of sperm and use of skilled inseminators. National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7344 10.14203/annalesbogorienses.2011.v15.n2.37-46 Annales Bogorienses; Vol. 15 No. 2 (2011): Annales Bogorienses; 37-46 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7344/5571 Copyright (c) 2011 Baharuddin Tappa https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7345 2026-10-19T03:56:45Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7345 10.14203/annalesbogorienses.2012.v16.n1.i Annales Bogorienses; Vol. 16 No. 1 (2012): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7345/5572 Copyright (c) 2012 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7346 2026-10-19T03:56:45Z annales:ART

Tissue Culture Propagation of “Hausa potato” (Solenostemon rotundifolius (Poir) JK Morton) Witjaksono, Witjaksono Leksonowati, Aryani Solanostemon rotundifolius hausa potato kentang hitam in vitro propagation acclimatization planting materials We have developed an effective plant propagation method of „hausa potato‟ through shoot proliferation in vitro, acclimatization in the greenhouse and transplantation of the plantlet on the individual media container for planting material production. The shoot cultures were initiated from shoots that grew from tuber collected from the field, disinfested using standard method and transferred to MS medium with 1 mg/l of BA for shoot proliferation. The proliferating shoots were then subcultured to MS medium with no growth regulators to induce rooting and enlarge the shoot. The plantlets were acclimatized in a plastic box containing medium of a mixture of cocopeat, sands, rice husk charcoal and top soil covered with plastic film to maintain humidity. The plantlets survived at 85% and almost 100% grew into planting materials ready for transplanting in the soil. The planting materials survived in the field and produced tuber normally.  National Research and Innovation Agency (BRIN) 2012-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7346 10.14203/ann.bogor.2012.v16.n1.1-8 Annales Bogorienses; Vol. 16 No. 1 (2012): Annales Bogorienses; 1-8 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7346/5573 Copyright (c) 2012 Witjaksono Witjaksono, Aryani Leksonowati https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7347 2026-10-19T03:56:45Z annales:ART

Isolation of cDNAs Encoding Asparagine Synthetase from Coronilla rostrata Artanti, Nina McFarlane, James cDNA nitrogen metabolism asparagine synthetase Coronilla rostrata Asparagine synthetase (AS) is one of the enzyme involves in ammonium metabolism in plant, it catalyzes the transfer of the amino group of glutamine to aspartate giving asparagine. Asparagine serves as a major nitrogen transport and storage molecule in many higher plants. This research used legume plant Coronilla rostrata callus culture for primary and secondary metabolism study. On primary metabolism, the culture were used to study the phenomenon of asparagine accumulation. Because of the responsiveness of the culture, we investigated the use of this culture for model system to conduct molecular biology study of the nitrogen metabolism. The aim of this study was to isolate cDNAs enconding asparagine synthetase from C. rostrata because of the asparagine accumulate in this culture. Several PCR based approaches were conducted such as RT-PCR, LM-PCR, and RACE. Using these methods, two AS cDNAs was isolated from C. rostrata AS1 (GenBank no AY081945) which a complete cDNA sequence and AS2 which a partial cDNA sequence. (GenBank no AF488726). These two cDNAs had high homology with the legume AS. National Research and Innovation Agency (BRIN) 2012-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7347 10.14203/ann.bogor.2012.v16.n1.9-18 Annales Bogorienses; Vol. 16 No. 1 (2012): Annales Bogorienses; 9-18 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7347/5574 Copyright (c) 2012 Nina Artanti, James McFarlane https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7348 2026-10-19T03:56:45Z annales:ART

Segregation Analysis of Transgenic Rice Plants cv Rojolele Harboring cry1B Gene and Plant Selection for Potential to Yellow Stem Borer Estiati, Amy Sulistyowati, Yuli Zahra, Fatimah Nurhasanah, Ade Nena Rachmawati, Syamsidah Loedin, Inez Hortenza Slamet bioassay cry1B gene transgenic rice yellow stem borer Transgenic rice plants harboring resistant gene to yellow stem borer, cry1B gene had been obtained. However, the cry1B gene in second generation of transgenic rice lines still segregating following Mendelian ratio 3:1. For further use in the breeding programs, it is important to ensure that the gene is dominant gene and the plants are homozygous for cry1B gene. Selection of homozygous transgenic rice lines containing cry1B gene at third, fourth and fifth generation had been conducted by PCR. The presence of cry1B gene was determined by showing the PCR product of 1.9 kb. Segregation analysis proved that six transgenic rice lines i.e. 3R7.8.15.1, 3R7.8.15.9, 3R7.8.15.10, 3R7.8.15.15, 3R7.8.15.21, 3R7.8.15.29 are homozygous lines for cry1B gene. Moreover, bioassay studies at vegetative stage on six homozygous transgenic rice lines showed that these transgenic rice lines are potential resistant to yellow stem borer comparing with non-transgenic plants, with the score of 0 (indicated no symptom) for six transgenic lines and score of 9 (more than 60% damaged tillers ) for non-transgenic plants. However, to confirm the efficacy of cry1B gene to yellow stem borer in natural condition, confined field trial in endemic area of yellow stem borer should be conducted.  National Research and Innovation Agency (BRIN) 2012-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7348 10.14203/ann.bogor.2012.v16.n1.19-25 Annales Bogorienses; Vol. 16 No. 1 (2012): Annales Bogorienses; 19-25 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7348/5575 Copyright (c) 2012 Amy Estiati, Yuli Sulistyowati, Fatimah Zahra, Ade Nena Nurhasanah, Syamsidah Rachmawati, Inez Hortenza Slamet Loedin https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7349 2026-10-19T03:56:45Z annales:ART

Cellulolytic Yeast Isolated From Raja Ampat Indonesia Kanti, Atit Sukarno, Nampiah Sukara, Endang Darusman, Latifah K. cellulolytic yeast Raja Ampat waste paper bamboo leaf The objective of this study was to select and characterize three yeast isolates originating from soil of Raja Ampat region of Papua, Indonesia for its potential to produce cellulase . Selection and characterization of cellulolytic yeast was carried out by measuring cellulolytic Index (IS)with congo red method and measurement of Carboxy Methyl Cellulase (CMC-ase) activity through determination of reducing sugar with dinitrosalycilic methods. Cellulolytic Index (IS) of the isolates Sporobolomyces poonsookiae Y08RA07, Rhodosporidium paludigenum Y08RA29, and Cryptococcus flavescens Y08RA33 were 1.40, 2.60 and 1.66, respectively. CMC-ase produced optimum at pH 8 at 37ºC by isolate Y08RA07; whereas for Y08RA29 and Y08RA33 were at pH 6, at 28ºC. Paper waste was good substrate for cellulase enzyme production by isolate Y08RA07, while for two other isolates the best substrate was CMC. Isolate Y08RA29 having highest cellulase activities when grown in CMC, while isolates Y08RA07 and Y08RA33 achieved highest enzyme activity when grown in bamboo leaf.  National Research and Innovation Agency (BRIN) 2012-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7349 10.14203/ann.bogor.2012.v16.n1.27-34 Annales Bogorienses; Vol. 16 No. 1 (2012): Annales Bogorienses; 27-34 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7349/5576 Copyright (c) 2012 Atit Kanti, Nampiah Sukarno, Endang Sukara, Latifah K. Darusman https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7350 2026-10-19T03:56:45Z annales:ART

Diversity of Actinomycetes from Soil Samples Collected from Lombok Island, Indonesia Lisdiyanti, Puspita Tamura, Tomohiko Ratnakomala, Shanti Ridwan, Roni Kartina, Gina Lestari, Yulin Ando, Katsuhiko Widyastuti, Yantyati actinomycetes Streptomyces Group Non-Streptomyces Group Diversity A total of 137 strains of Actinomycetes were isolated from 14 soil samples collected in 3 part of Lombok Island, Indonesia using SDS-YE (SY) and Rehydration and Centrifugation (RC) isolation methods and Humic Acid Vitamin (HV) agar as isolation media. All the isolates were identified by morphological characteristic and by analysis of 16S rRNA gene sequence. On the basis of their morphology and 16S rRNA gene sequence, 67% of isolates were belonged to the Streptomyces Group and 33% of isolates were belonged to the Rare-Actinomycetes (Non-Streptomyces) Group. Nine families and 15 genera were found from the samples. It is indicated the richness of actinomycetes in these area. The genus Streptomyces is the most abundant in all soil samples, occupying 67% of all isolates by using SY method; while the genus Actinoplanes is mainly found by using RC as isolation method. Two strains belong to the genus Virgisporangium and Catenulispora that showed taxonomic interest need further study for describing as ne species. National Research and Innovation Agency (BRIN) 2012-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7350 10.14203/ann.bogor.2012.v16.n1.35-40 Annales Bogorienses; Vol. 16 No. 1 (2012): Annales Bogorienses; 35-40 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7350/5577 Copyright (c) 2012 Puspita Lisdiyanti, Tomohiko Tamura, Shanti Ratnakomala, Roni Ridwan, Gina Kartina, Yulin Lestari, Katsuhiko Ando, Yantyati Widyastuti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7351 2026-10-19T03:56:45Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7351 10.14203/annalesbogorienses.2012.v16.n2.i Annales Bogorienses; Vol. 16 No. 2 (2012): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7351/5578 Copyright (c) 2012 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7352 2026-10-19T03:56:45Z annales:ART

Expression of an Anti-Transferrin Receptor Antibody scFV Fragment in Escherichia coli Using A L-Rhamnose-Based Tightly Regulated Promoter System Andriani, Dian Handayani, Ira Kusharyoto, Wien antibody fragment L-L-rhamnose Rhamex vector scFv transferrin receptor Transferrin receptor (TfR) levels are elevated in various types of cancer cells and correlate with the aggressive or proliferative ability of tumor cells. Therefore, TfR levels are considered useful as a prognostic tumor marker, and TfR is a potential target for drug delivery in therapy of malignant cells. In such kind of targeted delivery system, antibody fragments are frequently used as targeting moiety. Here, we report the generation of an anti-TfR single-chain antibody variable (scFv). The cDNA encoding the variable heavy and light chain domains of the scFv antibody fragment was derived from the anti-TfR monoclonal antibody LUCA31. The gene encoding the anti-TfR scFv fragment was codon optimized for expression in Escherichia coli, subsequently synthesized, and cloned into the pJExpress-804 Rhamex vector. The expression vector utilizes the E. coli rhaB promoter and corresponding regulatory genes, and is tightly regulated by the presence of L-L-rhamnose, it is also tightly regulated in the absence of L-L-rhamnose by the addition of D-glucose The His6-tagged anti-TfR scFv fragment was expressed in E. coli NiCo21 and purified by means of immobilized metal chelate affinity chromatography on TALON™ matrix. In SDS-PAGE, a single band corresponding to a molecular mass of approximately 30 kDa was observed which corresponded to the predicted molecular mass based on the amino acid sequence. National Research and Innovation Agency (BRIN) 2012-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7352 10.14203/ann.bogor.2012.v16.n2.1-6 Annales Bogorienses; Vol. 16 No. 2 (2012): Annales Bogorienses; 1-6 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7352/5579 Copyright (c) 2012 Dian Andriani, Ira Handayani, Wien Kusharyoto https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7353 2026-10-19T03:56:45Z annales:ART

Improved Regeneration, Acclimatization and Shoot Cutting Production of “Gebang” Cassava Derived from Irradiated In Vitro Shoots Supatmi, Supatmi Sudarmonowati, Enny cassava Gebang irradiated shoot young stem cutting/ratooning acclimatization in vitro Gebang is an Indonesian local genotype which has been selected as superior genotype for low amylose cassava. Prior to induction of new mutants of this genotype, series of research have been conducted to improve regeneration and acclimatization as well as shoot cuttings production of irradiated in vitro shoots. Four dosage treatments of gamma ray irradiation i.e. 0; 0.2; 1.0; 1.5; 2.0 krad were applied to 32 in vitro shoots multiplied from apical shoots of plants in the field. The highest multiplication rate and acclimatized shoots were obtained from shoots irradiated with 0.2 krad multiplied on various level of BAP added on MS medium and resulted that MS supplemented with 1 mg/L BAP was the best medium. The phenotypic variation was observed in shoots irradiated with 1.0; 1.5 and 2.0 krad while that irradiated with 0.2 krad performed normal appearances. Subsequent production of propagated young stem cuttings so called “ratooning system” decreased after the second cycle of propagation especially in the survival rate of that irradiated with 0.2 krad. The findings lead to the opportunity to produce cassava propagules derived from irradiated in vitro culture at a higher amount using Gebang genotype as a model. National Research and Innovation Agency (BRIN) 2012-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7353 10.14203/ann.bogor.2012.v16.n2.7-12 Annales Bogorienses; Vol. 16 No. 2 (2012): Annales Bogorienses; 7-12 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7353/5580 Copyright (c) 2012 Supatmi Supatmi, Enny Sudarmonowati https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7354 2026-10-19T03:56:45Z annales:ART

The Effect of Increase in NaCl Concentration on Growth and Proline Content of Purple Yam (Dioscorea alata L.) Grown In Vitro Martin, Andri Fadillah Azizah, Farroh Wulandari, Dyah Retno Ermayanti, Tri Muji purple yam Discorea alata L. NaCl in vitro growth proline content Tuber of purple yam (Dioscorea alata L.) has been used as an alternative food in some areas in Indonesia. The tuber contains high carbohydrate, low glycemic index and gluten free, therefore, study on genetic improvement of this species is needed to increase the productivity and to find out new cultivars which can be cultivated in marginal lands. This research was aimed to investigate the effect of NaCl concentration on growth and proline content of purple yam grown in vitro. Shoot tips were cultured on MS (Murashige and Skoog) medium supplemented with NaCl at concentrations of 25; 50; 100; 200 and 250 mM. After six weeks in culture, height of shoots, number of nodes, number of leaves, as well as proline content were recorded. The results showed that shoots grown on MS medium supplemented with NaCl at 25 and 50 mM had better growth compared to control. The best medium for its growth was MS containing 50 mM of NaCl. Increase in NaCl level’s resulted in decrease of growth. The LD50 value was obtained at 183 mM of NaCl. The Highest proline concentration was achieved by shoots grown on the medium supplemented with 100 mM of NaCl. This result indicated that purple yam was tolerant to the increase of NaCl concentration up to 100 mM, on MS medium without addition of plant growth regulators. National Research and Innovation Agency (BRIN) 2012-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7354 10.14203/ann.bogor.2012.v16.n2.13-18 Annales Bogorienses; Vol. 16 No. 2 (2012): Annales Bogorienses; 13-18 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7354/5581 Copyright (c) 2012 Andri Fadillah Martin, Farroh Azizah, Dyah Retno Wulandari, Tri Muji Ermayanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7355 2026-10-19T03:56:45Z annales:ART

Identification of Cadalene-β-Carboxylic Acid from Barks of Bawang Hutan (Scorodocarpus borneensis Becc.) Kartika, Rudi Bustanussalam, Bustanussalam Farida, Farida Simanjuntak, Partomuan bawang hutan Scorodocarpus borneensis Olaceaeae brine shrimp lethality test One sesquiterpene compound has isolated and identified from ethylacetate extract of bawang hutan (Scorodocarpus borneensis Becc.) barks. The barks were macerated with methanol, and then partitioned with mixture of ethylacetate-water (1:1). Fractionation of the ethylacetate phase by column chromatography gave pure compound. Based on data interpretation from of ultra-violet (UV) spectra, Fourier Transform Infra Red (FT-IR), NMR 1D (1H and 13C-NMR); NMR 2D (HMQC, COSY, HMBC) and comparison with literature, the pure isolated compound was determined as a sesquiterpene compound, cadalene-β-carboxylic acid which exhibit LC50 of 42.32 ppm. National Research and Innovation Agency (BRIN) 2012-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7355 10.14203/ann.bogor.2012.v16.n2.19-22 Annales Bogorienses; Vol. 16 No. 2 (2012): Annales Bogorienses; 19-22 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7355/5582 Copyright (c) 2012 Rudi Kartika, Bustanussalam Bustanussalam, Farida Farida, Partomuan Simanjuntak https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7356 2026-10-19T03:56:45Z annales:ART

Chemical Compositions and Antibacterial Effect of Essential Oil of Key Lime Leaves (Citrus aurantifolia Swingle: Rutaceae) Jamal, Yuliasri Pariwidjayanti, Aris Medta Agusta, Andria essential oil antibacteria key lime Citrus aurantifolia Swingle MIC Steam distillation of essential oil from fresh key leaves of Key lime (Citrus aurantifolia Swingle) gave 0.4% yellow essential oil with distinctive fragrance. Five out of 40 components of the essential oil were identified as the major components with content above 5%, they were geranial (103%). limonene (10.2%), neral (8.94%), caryphyllene (5.72%), and citronellal (5.41%). The essential oil was active against four bacterial test, Bacillus subtilis NBRC 3134, Staphylococcus aureus NBRC 14276, Micrococcus luteus NBRC 14218, and Escherichia coli NBRC 14237. B. subtilis was the most sensitive bacterium with the widest inhibition area at 50% concentration of essential oil. Minimum Inhibition Concentration (MIC) value of essential oil against B. subtilis was 0.125%. National Research and Innovation Agency (BRIN) 2012-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7356 10.14203/ann.bogor.2012.v16.n2.23-28 Annales Bogorienses; Vol. 16 No. 2 (2012): Annales Bogorienses; 23-28 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7356/5583 Copyright (c) 2012 Yuliasri Jamal, Aris Medta Pariwidjayanti, Andria Agusta https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7363 2026-10-19T03:56:45Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7363 10.14203/annalesbogorienses.2013.v17.n1.i Annales Bogorienses; Vol. 17 No. 1 (2013): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7363/5590 Copyright (c) 2013 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7364 2026-10-19T03:56:45Z annales:ART

Growth and Phycocyanin Productitivity of Spirulina fusiformis under Various Light Regimes Chrismadha, Tjandra Waluya, Rizky Agus A blue green algae National Research and Innovation Agency (BRIN) 2013-06-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7364 10.14203/ann.bogor.2013.v17.n1.1-6 Annales Bogorienses; Vol. 17 No. 1 (2013): Annales Bogorienses; 1-6 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7364/5591 Copyright (c) 2013 Tjandra Chrismadha, Rizky Agus Waluya https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7365 2026-10-19T03:56:45Z annales:ART

Identification of Differentially Expressed cDNA in Cassava under Drought Stress Using cDNA-RAPD Approach Hartati, N. Sri Supatmi, Supatmi Aryaningrum, Pramesti Dwi Sudarmonowati, Enny Cassave is an important carbohydrate National Research and Innovation Agency (BRIN) 2013-06-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7365 10.14203/ann.bogor.2013.v17.n1.7-14 Annales Bogorienses; Vol. 17 No. 1 (2013): Annales Bogorienses; 7-14 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7365/5592 Copyright (c) 2013 N. Sri Hartati, Supatmi Supatmi, Pramesti Dwi Aryaningrum, Enny Sudarmonowati https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7367 2026-10-19T03:56:45Z annales:ART

Isolation of Endophytic Microbes from Gunung Halimun National Park, West Java, Indonesia and Bioassay Their Potency for Eradicating Microbial Crops Pathogen Sukara, Endang Melliawati, Ruth Gunung Halimun National Park National Research and Innovation Agency (BRIN) 2013-06-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7367 10.14203/ann.bogor.2013.v17.n1.15-23 Annales Bogorienses; Vol. 17 No. 1 (2013): Annales Bogorienses; 15-24 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7367/5593 Copyright (c) 2013 Endang Sukara, Ruth Melliawati https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7368 2026-10-19T03:56:45Z annales:ART

Isolation of Microorganisms and Its Application for Decolorization of Anthraquinone and Azo Dyes from Textile Wastewater Fathoni, Ahmad Jeong, Soo Kyoung Kim, Joon Kyun The treatment of textile waste water National Research and Innovation Agency (BRIN) 2013-06-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7368 10.14203/ann.bogor.2013.v17.n1.25-31 Annales Bogorienses; Vol. 17 No. 1 (2013): Annales Bogorienses; 25-32 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7368/5595 Copyright (c) 2013 Ahmad Fathoni, Soo Kyoung Jeong, Joon Kyun Kim https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7369 2026-10-19T03:56:45Z annales:ART

Polyaromatic Hydrocarbon Degradation and Dioxygenase Gene Detection from Alteromonas alvinellae Bt05 Thontowi, Ahmad Rahmani, Nanik Yopi, Yopi Bto5 is marine bacterium National Research and Innovation Agency (BRIN) 2013-06-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7369 10.14203/ann.bogor.2013.v17.n1.33-42 Annales Bogorienses; Vol. 17 No. 1 (2013): Annales Bogorienses; 33-42 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7369/5596 Copyright (c) 2013 Ahmad Thontowi, Nanik Rahmani, Yopi Yopi https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7401 2026-10-19T03:56:45Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7401 10.14203/annalesbogorienses.2013.v17.n2.i Annales Bogorienses; Vol. 17 No. 2 (2013): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7401/5627 Copyright (c) 2013 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7402 2026-10-19T03:56:45Z annales:REV

Enhancing the Immunogenicity of Subunit Vaccines by Utilisation of Particulate Vaccine Delivery Systems Prasetyoputri, Anggia Kusharyoto, Wien immune system vaccine particulate delivery system liposome virus-like particles polymeric nanoparticles Control and eradication of a number of infectious diseases are primarily attributed to effective vaccination programs. A concerted effort is still imperative to develop novel vaccines and improve the immunogenicity of existing ones with regards to efficacy, immunogenicity and safety. Rational design of vaccines using subunit vaccines is a potentially safer alternative to conventional vaccines, yet they are poorly immunogenic without additional adjuvant. Using antigen carriers to enhance their immunogenicity in the forms of adsorption or encapsulation with a delivery system has been widely investigated as an alternative to currently available adjuvants. This review aims to elaborate on the existing nanotechnology being used to develop more immunogenic subunit vaccines, with focus on particulate delivery systems for development of prophylactic vaccine candidates.  National Research and Innovation Agency (BRIN) 2013-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7402 10.14203/ann.bogor.2013.v17.n2.1-16 Annales Bogorienses; Vol. 17 No. 2 (2013): Annales Bogorienses; 1-16 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7402/5628 Copyright (c) 2013 Anggia Prasetyoputri, Wien Kusharyoto https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7403 2026-10-19T03:56:45Z annales:ART

Insect Bioassay in Biosafety Containment to Select Transgenic Rice (Oryza sativa L.) Harboring Cry1B Gene Resistant to Yellow Stem Borer (Scrirpophaga incertulas Walk.) Estiati, Amy Nurhasanah, Ade Nena Nugroho, Satya transgenic rice cry1B gene insect bioassay Rojolele yellow stem borer Development of rice varieties resistant to yellow stem borer (YSB) is very crucial. Agrobacterium-mediated transformation of cry1B gene under wound inducible gene promoter mpi (maize proteinase inhibitor) into a local rice variety Rojolele had been conducted. PCR analysis proved that cry1B gene had been integrated into plant genome of 3R25 and 3R5 rice lines. Segregation analysis using PCR for cry1B gene of the two putative transgenic rice lines at third (T2), fourth (T3), fifth (T4), and sixth (T5) generations of 3R25 and 3R5 lines proved that 3R25.7.27, 3R25.7.13.8.2, 3R25.7.13.8.6, 3R25.7.13.8.8, 3R5.26.2, and 3R5.26.5 are homozygous lines for cry1B gene. Insect bioassay on three randomly picked homozygous transgenic rice lines to study the efficacy of cry1B gene toward YSB was conducted in biosafety containment by infestingYSB larvae at first instar into 3R25.7.27, 3R25.7.13.8.6, and 3R5.26.5 transgenic rice lines, using non--transgenic Rojolele, IR64 and IR74 as susceptible controls. The results showed that the percentages of deadhearts symptoms of 3R25.7.27, 3R5.26.2, and 3R25.7.13.8.6 rice lines were lower than those of the susceptible control lines with scores of 0.1 and 0, respectively. While the scores of all three susceptible control plants were 9. The results proved that lines 3R25.7.27, 3R25.7.13.8.6, and 3R5.26.2 were categorized as resistant lines while the non-transgenic Rojolele, IR64, and IR74 were categorized as susceptible lines. The results also showed that the cry1B gene was expressed and produced insecticidal protein CRY1B which were active against YSB to protect rice plant toward YSB infestation.  National Research and Innovation Agency (BRIN) 2013-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7403 10.14203/ann.bogor.2013.v17.n2.17-26 Annales Bogorienses; Vol. 17 No. 2 (2013): Annales Bogorienses; 17-26 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7403/5629 Copyright (c) 2013 Amy Estiati, Ade Nena Nurhasanah, Satya Nugroho https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7404 2026-10-19T03:56:45Z annales:ART

Detection of Keto Synthase (KS) Gene Domain in Sponges and Bacterial Sponges Atikana, Akhirta Naim, Mohamad Azrul Sipkema, Detmer sponges bacterial sponges secondary metabolite PKS gene ketosynthase Sponges are sources of various useful natural products. The natural compounds derived from sponges are mostly complex polyketide. The polyketide synthase genes (PKS) is responsible for the biosynthesis of complex polyketides. During this research, we detected ketosynthase (KS) gene domain from 10 species of marine sponges, collected from Mediterranean Sea (Aplysina aerophoba, Petrocia ficiformis, Axinella damicornis, Axinella veronicas, Crambe crambe) and North Sea (Halichondria panicea, Haliclona xena, Haliclona oculata, Suberites massa, Ephydatia fluviatilis). We also detected KS gene domain from 12 bacterial sponge isolated from H. panicea. We used a PCR-based approach to detect the ketosynthase (KS) gene domain of Polyketosynthase (PKS) genes. Our result showed that the sponges and the bacterial sponges are mostly KS positive (700 bp). The presence of the PKS gene indicates the ability of the sponges to produce bioactive compound and potential natural product. The presence of the KS domain in both of the sponges and the bacterial sponges might also indicate that the bacterial sponges involved in the biosyntesis of secondary metabolite of the sponges.  National Research and Innovation Agency (BRIN) 2013-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7404 10.14203/ann.bogor.2013.v17.n2.27-33 Annales Bogorienses; Vol. 17 No. 2 (2013): Annales Bogorienses; 27-33 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7404/5630 Copyright (c) 2013 Akhirta Atikana, Mohamad Azrul Naim, Detmer Sipkema https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7405 2026-10-19T03:56:45Z annales:ART

Potency of Endophyte Bacterium Isolated from Shorea selanica on Producing IAA Hormone and Supporting the Growth of Soybean Widowati, Tiwit Nuriyanah, Nuriyanah Sukiman, Harmastini endophytes bacteria Indole Acetic Acid soybean stress test Growth of soybean plants was supported by the presence of nitrogen fixing bacteria. Besides nitrogen, other elements such as phosphate, potassium and growth hormones are also required. Endophytic bacteria associated with Shorea selanica were isolated and tested for their ability to produce indole acetic acid (IAA) hormone and exhibit stress tolerance. Colorimetric analysis showed that isolate SSBt2 produced the highest IAA (43.01 μg/ml) in culture supplemented with L-tryptophan. Isolate SSBt2 grew well in the some stress tests, except on heat and oxidative stress. The highest IAA producing strain was selected for determining its capability and compatibility to support the growth of soybean plants in glass house experiment. The results indicated that the endophytic bacteria isolated from S. selanica are compatible to support the growth of soybean. SSBt2 was identified as Enterobacter hormaechei based on 16S rRNA gene analysis. National Research and Innovation Agency (BRIN) 2013-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7405 10.14203/ann.bogor.2013.v17.n2.35-41 Annales Bogorienses; Vol. 17 No. 2 (2013): Annales Bogorienses; 35-41 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7405/5631 Copyright (c) 2013 Tiwit Widowati, Nuriyanah Nuriyanah, Harmastini Sukiman https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7406 2026-10-19T03:56:45Z annales:ART

Isolation and Screening of Surfactant-producing Bacteria from Indonesian Marine Environments and Its Application on Bioremediation Susilaningsih, Dwi Okazaki, Fumiyoshi Yopi, Yopi Widyastuti, Yantyati Harayama, Shigeaki bacteria biosurfactant screening improvement of soil texture Isolation and screening have been undertaken on oil-degrading microbes from Indonesian marine environments. During screening process it has been found many bacterial isolates capable of degrading crude oil. Hence, study has been focused on the biodiversity of biosurfactant-producing bacterial species in Indonesian marine environment and its function for remedial the pollutant in marine and soil areas. A total of 103 out of 463 isolates showed positive surfactant-degrading properties. By means of partial 16S rRNA gene analyses, it has been found that the majority of taxa are related to Alcanivorax, Pseudomonas, Bacillus, Bortetela, Brucella, Acenitobacter, Staphia, Lysobacter, and Talasosophira. Biosurfactant properties assay showed that they were capable of lowering the surface and interfacial water tension from 74 mN/m to 40-65 mN/m and from 24 mN/m to 6-10 mN/m, respectively. In addition, most of the surfactants were capable of emulsifying hydrocarbon (crude oil) of 0.01 to 0.15 units, comparable to 0.08 units of synthetic surfactant (20% Tween). Further observation showed that the majority of the surfactants were able to degrade a long chain of alkane, but not branched alkane, with a recovering rate of 20-80%. The application of the surfactant towards oil polluted model beach was done in laboratory scale and showing the surfactant obtained from microbial broth cultures capable for recovering the oil pollutant significantly, compared to the control (without addition microbial broth).  National Research and Innovation Agency (BRIN) 2013-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7406 10.14203/ann.bogor.2013.v17.n2.43-53 Annales Bogorienses; Vol. 17 No. 2 (2013): Annales Bogorienses; 43-53 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7406/5632 Copyright (c) 2013 Dwi Susilaningsih, Fumiyoshi Okazaki, Yopi Yopi, Yantyati Widyastuti, Shigeaki Harayama https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7409 2026-10-19T03:56:45Z annales:ART

Antioxidant, Antibacterial, and Antidiabetic Activities of Roselle (Hibiscus sabdariffa) Extracts Suharli, Lili Manguntungi, Baso Azis, Andi Asmawati Mustopa, Apon Zaenal Meilina, Lita Pandayu, Iga Firmansyah Vanggy, Leggina Rezzy Irawan, Shasmita antibacterial activity antioxidant activity antidiabetic activity roselle pharmacological bioprospection Hibiscus sabdariffa L., also referred to as roselle, is commonly utilized in the pharmaceutical and food industries. Roselle contains bioactive compounds such as phenolics, alkaloids, tannins, flavonoids, saponins, and organic acids, which have pharmacological properties, such as antioxidant, antibacterial, immune booster, antidiabetic, anti-inflammatory, and anti-hypertensive properties. There are many studies regarding the pharmacological activities of roselle extract and its applications. However, there has been no research to study the effectiveness of the solvent in testing roselle petal extracts against antibacterial, antioxidant, and antidiabetic activities, simultaneously. This research used two kinds of polar solvents, dH2O and ethanol, with various concentrations for antibacterial activity test by five pathogenic bacteria, for antioxidant test by the DPPH method, and for antidiabetic test by the alpha-glucosidase inhibition method. The result showed that the ethanol extract of roselle had higher antibacterial activity compare to the roselle water extract. Antioxidant activity of roselle ethanol extract at 20% concentration had the highest activity 69.75 ± 0.002%; while, the 100% concentration of roselle water extract had the highest antioxidant activity 138.73 ± 0.013%. antidiabetic activity of roselle ethanol and water extract at 100% concentration had the highest activity 1,195.44 ± 0.007% and 1,552.49 ± 0.069%, respectively. National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7409 10.14203/ann.bogor.2021.v25.n2.82-88 Annales Bogorienses; Vol. 25 No. 2 (2021): Annales Bogorienses; 82-88 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7409/5637 Copyright (c) 2026 Lili Suharli, Baso Manguntungi, Andi Asmawati Azis, Apon Zaenal Mustopa, Lita Meilina, Iga Firmansyah Pandayu, Leggina Rezzy Vanggy, Shasmita Irawan https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7413 2026-10-19T03:56:45Z annales:ART

Isolation and Characterization of Bacterial Carbonate Precipitation for Biogrouting Suyanto, Eko Sari, Miranti Nurindah Ratnakomala, Shanti Fahrurrozi, Fahrurrozi Gusmawati, Niken F. calcium carbonate precipitation bacteria urease biogrouting Bacilli Biogrouting is a process that transforms soil or sand into calcarenite or sandstone by bacterial calcium carbonate precipitation . The mechanisms of this process are that the bacteria hydrolyze urea catalyzed by urease, and with the existence of dissolved Ca2+, a solid crystalline calcite or calcium carbonate (CaCO3) will be produced. The main advantage of biogrout is that soil or sand can be strengthened without interfering with the hydraulics of the treated soil or sand and without excavation or replacement. In this study, the isolation and identification of bacterial calcium carbonate precipitation , and characterization of urease produced by bacteria were conducted. In the isolation method, the enrichment method using urea as abacterial carbon source was carried out. The formation of crystalline calcite was observed by a light microscope. The urease enzyme activity was determined by the 3, 5-Dinitrosalicylic acid (DNS) method. The molecular identification of isolates was analyzed by the determination of 16S rRNA gene sequencing. As a result, 19 calcium carbonate precipitation have been isolated from soils, sands, water, and rocks collected from several areas in Indonesia. They showed calcite formation in a medium with urea. Molecular identification of isolates with high urease enzyme activity revealed that the isolates belong to the group Bacilli. The highest enzyme activity produced by ID10-U004 is 374.94 U/mL. The preliminary biogrouting experiment was also conducted using isolated strain, and the reaction was able to make the sand solid and stiff. National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7413 10.14203/ann.bogor.2021.v25.n2.89-97 Annales Bogorienses; Vol. 25 No. 2 (2021): Annales Bogorienses; 89-97 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7413/5640 Copyright (c) 2026 Eko Suyanto, Miranti Nurindah Sari, Shanti Ratnakomala, Fahrurrozi Fahrurrozi, Niken F. Gusmawati https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7656 2026-10-19T03:56:45Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7656 10.14203/annalesbogorienses.2014.v18.n1.i Annales Bogorienses; Vol. 18 No. 1 (2014): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7656/5842 Copyright (c) 2014 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7657 2026-10-19T03:56:45Z annales:REV

Role of Lactobacillus helveticus on Flavor Formation in Cheese: Amino Acid Metabolism Widyastuti, Yantyati Lisdiyanti, Puspita Tisnadjaja, Djadjat Lactobacillus helveticus amino acids catabolism aromatic amino acid branched-chain amino acid sulphur compound cheese flavor Lactic acid bacteria, mainly lactobacilli, play an important role in cheese making. Their role can be divided into starters and non-starters or secondary microorganisms. Lactobacillus helveticus, an obligately homofermenter and thermophilic bacterium, has unique properties as a starter because of its ability to induce strong impact on cheese flavor. The bacteria are known to be prototrophic for 5 amino acids and auxotrophic for 13 amino acids. It is interesting that the conversion of aromatic amino acids, branch chain amino acids, and methionine into volatile and nonvolatile compounds by L. helveticus is thought to represent the rate-limiting step in the formation of mature flavor and aroma in cheese. The addition of a highly autolytic L. helveticus to a starter system could significantly increase the formation of flavor precursor and some volatile compounds during cheese ripening. This article focuses on the contribution of L. helveticus to flavour compound formation in cheese with particular emphasis on amino acid metabolism. National Research and Innovation Agency (BRIN) 2014-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7657 10.14203/ann.bogor.2014.v18.n1.1-11 Annales Bogorienses; Vol. 18 No. 1 (2014): Annales Bogorienses; 1-11 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7657/5843 Copyright (c) 2014 Yantyati Widyastuti, Puspita Lisdiyanti, Djadjat Tisnadjaja https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7658 2026-10-19T03:56:45Z annales:ART

Construction and Expression of Immunotoxin Anti EGFRvIII scFv-HPR Conjugate in Pichia pastoris as A Targeted Drug Candidate for Cancer Therapy Yuliawati, Yuliawati Soejoedono, Retno Damayanti Fuad, Asrul Muhamad EGFRvIII HPR (human pancreatic ribonuclease) immunotoxin conjugate anti-EGFRvIII scFv Pichia pastoris Epidermal growth factor receptor variant III (EGFRvIII) is a mutant of EGFR lacking 267 amino acids (exon-2 through 7) within its extracellular domain, resulting in the formation of a new epitope as a tumor specific target. EGFRvIII is commonly found in GBM, breast, ovarian, prostate, and lung carcinomas. Antibodies or part of antibodies (e.g. single chain variable fragment or scFv) with specific activity against this receptor have been developed. These antibodies are internalized into the cell after receptor binding. Ribonucleases can be cytotoxic due to their inherent ability to degrade RNA, therefore causing cell death via inhibition of protein synthesis. The aim of this research is to construct, clone and express an immunotoxin-based conjugate combining an anti-EGFRvIII scFv with a HPRmut (human pancreatic ribonuclease mutant variant) in Pichia pastoris. The anti-EGFRvIII scFv gene was cloned into yeast expression vector pPICZαA and fused with EGFP gene as a marker. The HPRmut gene was subsequently cloned at the C-terminal of the scFv::EGFP fusion, resulting in the scFv::EGFP::HPR fusion construct. The fusion construct was successfully obtained and nucleotide sequence of plasmid was verified. This construct was used to transform P. pastoris SMD 1168H. The gene fusion was successfully transformed and expressed in P. pastoris with a transformation efficiency of 102 cfu/μg DNA. The transformed yeasts were screened on agar media containing up to 1000 μg/ml zeocin. Yeast transformants showed green fluorescent due to the expression of EGFP gene. The recombinant proteins have been expressed and secreted from P. pastoris as showed by immunoblotting and SDS-PAGE analyses, then purified by affinity chromatography method. The selected yeast transformant produced at least 15.85 mg of purified protein per liter of yeast culture.  National Research and Innovation Agency (BRIN) 2014-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7658 10.14203/ann.bogor.2014.v18.n1.13-23 Annales Bogorienses; Vol. 18 No. 1 (2014): Annales Bogorienses; 13-23 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7658/5844 Copyright (c) 2014 Yuliawati Yuliawati, Retno Damayanti Soejoedono, Asrul Muhamad Fuad https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7659 2026-10-19T03:56:46Z annales:ART

Expression of An Immunogenic Intimin Fragment of EHEC O157:H7 in Escherichia coli Periplasm under The Control of A Rhamnose-Based Regulated Promoter Hariyatun, Hariyatun Suwanto, Antonius Kusharyoto, Wien EHEC O157:H7 immunogenic Intimin fragment E. coli periplasm rhamnose-based regulated promoter Intimin is the main adhesin of Enterohemorrhagic E. coli (EHEC) O157:H7 bacteria which are the most common leading infectious cause of bloody diarrhea and acute kidney failure in children who develop hemolytic uremic syndrome (HUS). Intimin is required for persistent bacterial colonization to eukaryotic host cell and its receptor-binding activity is localized at the C-terminus 282 amino acids (Intimin282). Thus, Intimin282 is an attractive antigen candidate that could be useful in vaccine and diagnostic systems against EHEC infections. Previous studies had reported expression of Intimin in E. coli cytoplasm using commonly used prokaryotic expression systems. However, it usually encountered several problems, i.e. low expression level, leaky expression, inclusion body formation, and truncated protein. The pRHA vector, which is tightly regulated by Lrhamnose and D-glucose, represents a viable alternative E. coli expression system to overcome such problems. Moreover, E. coli periplasm has an advantage of maintaining protein functionality by providing an oxidative environment that is more efficient than cytoplasm. However, to date there is no study about Intimin expressionusing pRHA expression system and/or in E. coli periplasm. Accordingly, we constructed a recombinant pRHA vector harbouring the respective gene to investigate the expression of an immunogenic Intimin fragment of EHEC O157:H7 in E. coli periplasm. The gene encoding His6-tagged Intimin282 (Int282) together with pelB signal sequence was cloned into the pRHA vector, subsequently expressed in E. coli JM109 and purified. Expression and purification of Int282 were verified by SDS-PAGE and Western blot. The result showed that Int282 was successfully expressed in E. coli periplasm with a protein size of approximately 32 kDa, which corresponded with the predicted size of the protein based on its amino acid sequence. National Research and Innovation Agency (BRIN) 2014-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7659 10.14203/ann.bogor.2014.v18.n1.25-34 Annales Bogorienses; Vol. 18 No. 1 (2014): Annales Bogorienses; 25-34 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7659/5846 Copyright (c) 2014 Hariyatun Hariyatun, Antonius Suwanto, Wien Kusharyoto https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7662 2026-10-19T03:56:46Z annales:ART

Somatic Embryo Germination of Jatropha curcas L in Presence of Sucrose and Poly Ethylene Glycol (PEG) Rudiyanto, Rudiyanto Efendi, Darda Ermayanti, Tri Muji Jatropha curcas germination somatic embryo sucrose polyethylene glycol (PEG) Jatropha curcas L. is a potential source of a non-edible biofuel. Conventional propagation of J. curcas technique has some limitations. Somatic embryo can produce a large number of embryos and obtain a large number of plants all year round. Treatment of sucrose in combination with polyethylene glycol (PEG) was proven to enhance germination of somatic embryos in many plant species. The aim of the study was to investigate the effect of sucrose in combination of PEG on somatic embryo germination in J. curcas. Globular somatic embryos at 0.025-0.030 g fresh weight having 0.4-0.5 cm in diameter were grown on MS medium solidified with 3 g/l of Gelzan supplemented with sucrose at 20, 30, 40, and 50 g/l in combination with PEG at 0, 2.5, 5, 10, and 15%. Results showed that the best medium for germination of J. curcas somatic embryo cultures was MS medium supplemented with 20 and 30 g/l of sucrose in combination with 5% of PEG. The numbers of germinated embryos per clump had significant enhancement on those medium compared with the control (PEG free treatment) (2.65 to 5.65) and (2.55 to 5.50). In addition, those treatments resulted in the highest percentage of clumps forming germinated embryos (100%), with an average of normal germinated embryos at 94.163 and 96.065%. The addition of 40 and 50 g/l of sucrose in combination with 15% of PEG caused all embryos to fail at germinating.  National Research and Innovation Agency (BRIN) 2014-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7662 10.14203/ann.bogor.2014.v18.n1.35-43 Annales Bogorienses; Vol. 18 No. 1 (2014): Annales Bogorienses; 35-43 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7662/5849 Copyright (c) 2014 Rudiyanto Rudiyanto, Darda Efendi, Tri Muji Ermayanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7664 2026-10-19T03:56:46Z annales:ART

In vitro Seed Germination and Shoot Multiplication of Seven Endemic Subalpine and Alpine Plant Species Grown on Mount Jaya, Papua, Indonesia Ermayanti, Tri Muji Hafiizh, Erwin Al Mandessy, Ary Setyadi, Gesang Mukhsia, Andi Tetramolopium klossii Deschampsia klossii Papuacalia cartenszensis Epilobium hooglandii Gaultheria novoguinensis Rhododendron correoides Rhododendron culminicolum in vitro germination shoot multiplication endemic alpine and sub-alpine Papua plants Exploitation on plant population may put the endemic plants into an endangered state, hence, these plants will need to be conserved. In order to pursue conservation on endemic plants, we conducted in vitro seed germination and shoot multiplication of seven alpine and sub-alpine species endemic to Mount (Mt.) Jaya, in Papua, Indonesia, i.e. Tetramolopium klossii, Deschampsia klossii, Papuacalia cartenszensis, Epilobium hooglandii, Gaultheria novoguinensis, Rhododendron correoides, and Rhododendron culminicolum. These species are categorized as slow-growth plants found in higher altitude (over 3700 m above sea level) and low temperature of Mt. Jaya. Seeds were surface-sterilized using Na-hypochloride and germinated aseptically on Murashige and Skoog (MS) medium. Dytikinin benzyl adenine (BA) was used for shoot multiplication. Seedling cultures were maintained in a controlled environment with continuous low light intensity (800 lux) and at temperature 26-27oC. Results showed that most species had more than 80% of germination rate on MS medium after a week in culture. BA was required to enhance shoots multiplication. Woody Plant (WP) (Lloyd & McCown, 1981) medium gave better shoot multiplication for R. culminicolum.  National Research and Innovation Agency (BRIN) 2014-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7664 10.14203/ann.bogor.2014.v18.n1.45-53 Annales Bogorienses; Vol. 18 No. 1 (2014): Annales Bogorienses; 45-53 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7664/5852 Copyright (c) 2014 Tri Muji Ermayanti, Erwin Al Hafiizh, Ary Mandessy, Gesang Setyadi, Andi Mukhsia https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7667 2026-10-19T03:56:46Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7667 10.14203/annalesbogorienses.2014.v18.n2.i Annales Bogorienses; Vol. 18 No. 2 (2014): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7667/5853 Copyright (c) 2014 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7668 2026-10-19T03:56:46Z annales:ART

Optimization of Culture Conditions for Production of β-Mannanase by Strain Nonomuraea sp. ID06-379 using Submerged Substrate Fermentation Ratnakomala, Shanti Yopi, Yopi Suhartono, Maggy Thenawidjaja Meryandini, Anja Prasetya, Bambang β-mannanase actinomycetes Nonomuraea sp. ID06-379 optimization medium substrate fermentation. The objective of this study was to investigate the effect of media compositions on the production of β-mannanase by Nonomuraea sp. ID06-379. The study was focused on the influence of carbon, nitrogen, phosphorus and detergents on β-mannanase synthesis through manipulating media compositions on production medium. The results indicated that for carbon sources, locus bean gum (0.745 ± 0.036 U/ml) showed maximum mannanase activity. Malt extract was the best nitrogen source for producing β-mannanase (1.075 ± 0.006 U/ml), (NH4)2HPO4 as phosphate source (1.733 ± 0.026 U/ml) and Tween 80 (1.145 ± 0.003 U/ml) as surfactants effect on increasing permeability of bacterial cell membrane, enhancing membrane transport and excretion of extracellular enzymes into the production media. The results showed that 1% malt extract, 0.5% locus bean gum and 0.05% (NH4)2HPO4 were good substances for nitrogen source, carbon source and phosphate respectively. The highest production of β-mannanase by Nonomuraea sp. ID06-379 (5.33 U/mg) was reached in the medium optimization (Vogel’s minimal medium) contained the following ingredients: 0.5% locus bean gum, 1% malt extract and 0.05% (NH4)2HPO4, under submerged fermentation with shaking at 120 rpm and 28C for 2 days incubation.  National Research and Innovation Agency (BRIN) 2014-12-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7668 10.14203/ann.bogor.2014.v18.n2.1-11 Annales Bogorienses; Vol. 18 No. 2 (2014): Annales Bogorienses; 1-11 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7668/5854 Copyright (c) 2014 Shanti Ratnakomala, Yopi Yopi, Maggy Thenawidjaja Suhartono, Anja Meryandini, Bambang Prasetya https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7669 2026-10-19T03:56:46Z annales:ART

Identification of nifD and nifH Genes of Methanotrophic Bacteria from Rice Field Bintarti, Ari Fina Rusmana, Iman Wahyudi, Aris Tri methanotrophic bacteria nitrogen fixation nitrogenase nifH nifD Metanotrophic bacteria have ability to oxidize methane and fix atmospheric nitrogen, hence the bacteria has an important role as a nitrogen source provider on wetland area like rice fields. Nitrogen fixation process is catalyzed by the nitrogenase enzyme complex, encoded by nifD and nifH genes. However, characteristic of these genes from indigenous-methanotrophic bacteria still poorly understood. Hence, nifD and nifH genes of methanotrophic bacteria isolated from rice fields in Indonesia (BGM3, BGM9, SS1, SS3, SS10, ST18, SP3, and INP4) were identified and characterized. Detection of nifH and nifD genes was conducted by polymerase chain reaction (PCR) amplification. nifH and nifD gene sequences were analyzed using BLAST-X and phylogenetic trees were constructed using Neighbour Joining method. Based on nifH sequences analysis, SS1 closely related to Beijerinckia mobilis and SS3, SS10, ST 18 closely related to Beijerinckia indica subsp. indica ATCC 9039, while, BGM3, INP4, and BGM9 related to nifH of uncultured nitrogen-fixing bacterium. In other hand, sequence analysis of nifD gene showed that SS1, SS3, SS10, ST 18 closely related to B. indica subsp. indicaATCC 9039 and BGM3, BGM9, INP4 closely related to Xanthobacter autotrophicus Py2. Identification by 16S rRNA gene indicated that SS1, SS3, SS10, and ST18 had closeness to Beijerinckia sp. P310-1, while INP4 closely related to Xanthobacter sp. M5C24. National Research and Innovation Agency (BRIN) 2014-12-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7669 10.14203/ann.bogor.2014.v18.n2.13-25 Annales Bogorienses; Vol. 18 No. 2 (2014): Annales Bogorienses; 13-25 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7669/5855 Copyright (c) 2014 Ari Fina Bintarti, Iman Rusmana, Aris Tri Wahyudi https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7670 2026-10-19T03:56:46Z annales:ART

Enhancement of β-Glucosidase Activity in Penicillium sp. by Random Mutation with Ultraviolet and Ethyl Methyl Sulfonate Syafriana, Vilya Nuswantara, Sukma Mangunwardoyo, Wibowo Lisdiyanti, Puspita β-glucosidase UV EMS Penicillium The genus Penicillium has a potential ability to produce β-glucosidase. The aim of the study was to improve the β-glucosidase activity of Penicillium sp. ID10-T065 with physical (Ultraviolet = UV), chemical (Ethyl Methyl Sulfonate = EMS), and combined mutation (UV-EMS). The spores of Penicillium sp. ID10-T065 were exposed into UV irradiation for 3 minutes with dose of 0.1 J/cm2 and 13 cm of distances. Chemical mutation was done by treated spores into 3% of EMS solution for an hour. Combined mutation of UV and EMS were also performed by UV for 3 minutes (0.1 J/cm2, 15 cm) and continued with soaking into 2-3% of EMS solution. The developed mutants were screened, selected and assayed. Comparison of enzyme activities with the wild- type (1.78 U/ml), mutant UV13 (5.53 U/ml) showed a 3.1 fold increase; mutant EM31 (4.26 U/ml) showed a 2.4 fold increase. Meanwhile, mutant UM23 obtained from the multiple exposures showed a decreased activity (1.75 U/ml). Mutant UV13 showed the best enzyme activity to be considered as a potential strain for β-glucosidase producer. This result needs to be further elaborated especially on its genetic stability studies in order for the ascertained as a stable mutant.  National Research and Innovation Agency (BRIN) 2014-12-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7670 10.14203/ann.bogor.2014.v18.n2.27-33 Annales Bogorienses; Vol. 18 No. 2 (2014): Annales Bogorienses; 27-33 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7670/5856 Copyright (c) 2014 Vilya Syafriana, Sukma Nuswantara, Wibowo Mangunwardoyo, Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7671 2026-10-19T03:56:46Z annales:ART

Preparation of An scFv-Based Immunoliposome Specific towards Transferrin Receptor Kusharyoto, Wien Handayani, Ira Sari, Martha Fuad, Asrul Muhamad antibody fragment codon optimization immunoliposome scFv transferrin receptor An ideal therapeutic for cancer would be one that selectively targets to tumor cells, is nontoxic to normal cells, and that could be systemically delivered, thereby reaching metastases as well as primary tumor. Immunoliposomes directed by monoclonal antibody or its fragments are promising vehicles for tumor targeted drug delivery. Transferrin receptors (TfR) levels are elevated in various types of cancer cells and considered to correlate with the aggressive or proliferative ability of tumor cells. Therefore, TfR levels can be elaborated as a prognostic tumor marker, and TfR is a potential target for drug delivery in the therapy of malignant cells. Here, we report the preparation of an anti-TfR single-chain antibody variable (scFv) immunoliposome for tumor targeted delivery vehicle. The cDNA encoding the variable heavy and light chain domains of the anti-TfRscFv antibody fragment was derived from the murine monoclonal antibody Clone E6, which is specific towards transferrin receptor. The gene encoding the anti-TfR scFv fragment was codon optimized for expression in Escherichia coli, subsequently synthesized, and cloned into the expression vector pJexpress404. The His6-tagged anti-TfR scFv fragment was expressed in E. coli and purified by means of immobilized metal-ion affinity chromatography on TALON™ matrix. SDS-PAGE revealed that the scFv fragment had the size of approximately 27 kDa, which corresponded with the predicted size of the protein based on its amino acid sequence. Liposome containing 5% MPB-DOPE were prepared by ethanol injection method. Afterwards, the anti-TfR scFv fragments were covalently conjugated to the liposome to produce the anti-TfR scFv immunoliposome with the size of around 200 to 300 nm.  National Research and Innovation Agency (BRIN) 2014-12-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7671 10.14203/ann.bogor.2014.v18.n2.35-44 Annales Bogorienses; Vol. 18 No. 2 (2014): Annales Bogorienses; 35-44 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7671/5857 Copyright (c) 2014 Wien Kusharyoto, Ira Handayani, Martha Sari, Asrul Muhamad Fuad https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7672 2026-10-19T03:56:46Z annales:ART

Isolation and Characterization of OsNAC6 cDNA from Rice (Oryza sativa L.) cv. Nipponbare, Batutegi, and Rojolele Rachmat, Agus Nugroho, Satya Nurdiani, Dini Swastika, Maria Sukma, Dewi Aswidinnoor, Hajrial Sudarsono, Sudarsono transcription factor stress responsive gene abiotic stress NAC Transcription factors have an important function in regulating gene expression and plant responses to stresses. The ERF, bZIP, WRKY, MYB, and NAC are stress inducible transcription factors. The OsNAC6 is a member of the NAC transcription factor family in rice and its expression is induced by abiotic stresses, wounding and blast disease. Characterization of OsNAC6 gene sequences would give a better understanding on how OsNAC gene functions biologically. The objectives of this research are to isolate the OsNAC6 cDNA from Nipponbare, Batutegi, and Rojolele cultivars, to characterize their DNA sequences, and to compare their sequences to other NAC genes from other plants available in GenBank DNA databases. Isolated cDNA and sequencing of the fragments resulted in a 912 bp DNA sequences. Translation of the sequences yielded a protein consisted of 303 amino acid residue. Blast analysis of amino acid sequences indicated identity of isolated cDNA from three Indonesian rice cultivars are the OsNAC6 gene. Deduced amino acid residues from amplified cDNAs of Nipponbare, Batutegi, and Rojolele cultivars shared 100% sequence identities to rice OsNAC6 (Acc. # BAA89800), 71-100% sequence identity to a number of OsNAC protein from Oryza sativa and 63-83% sequence identity to NAC protein from other plants.  National Research and Innovation Agency (BRIN) 2014-12-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7672 10.14203/ann.bogor.2014.v18.n2.45-55 Annales Bogorienses; Vol. 18 No. 2 (2014): Annales Bogorienses; 45-55 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7672/5858 Copyright (c) 2014 Agus Rachmat, Satya Nugroho, Dini Nurdiani, Maria Swastika, Dewi Sukma, Hajrial Aswidinnoor, Sudarsono Sudarsono https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7675 2026-10-19T03:56:46Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7675 10.14203/annalesbogorienses.2015.v19.n1.i Annales Bogorienses; Vol. 19 No. 1 (2015): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7675/5859 Copyright (c) 2015 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7676 2026-10-19T03:56:46Z annales:ART

Optimization Expression and Stability Test of Recombinant Human Interferon Alfa 2a Fusion Protein in Escherichia coli BL21 (DE3) Santoso, Adi Kusumawati, Arizah rhFN α2a fusion protein E. coli BL21 (DE3) expression stability test The rhIFN α2a is expressed as a fusion protein containing thioredoxine and polyhistidine sites at its N terminal. Our previous research has obtained recombinant human IFN α2a (rhIFN α2a) protein that expressed predominantly as a soluble form in E. coli BL21 (DE3). Through systematic approach of various culture conditions, the aim of current this research is to acquire the best condition and its stability of recombinant rhIFN α2a fusion protein in a culture under study. Expression optimization performed by using three parameters, i.e.: temperature, induction time and inducer concentration. Various IPTG concentrations are 0.25, 0.5, 0.75, and 1.0 mM. The incubation time of bacterial cell culture carried out in 3, 4, and 5 hours at temperature 28, 30, and 37°C. The best condition was used to analyze the stability of rhIFN α2a protein expression up to ten generation. The expressed protein was analyzed using SDS PAGE and CBB staining. The optimal culture condition was found to be 37 °C temperature with 4 hours time of induction and 1 mM IPTG concentration. Stability analysis revealed that the rhFN α2a protein expression remained stable until the tenth generation with molecular weight, approximately, 36 kDa.  National Research and Innovation Agency (BRIN) 2015-06-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7676 10.14203/ann.bogor.2015.v19.n1.1-8 Annales Bogorienses; Vol. 19 No. 1 (2015): Annales Bogorienses; 1-7 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7676/5860 Copyright (c) 2015 Adi Santoso, Arizah Kusumawati https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7677 2026-10-19T03:56:46Z annales:ART

Optimizaton of Cationic Lipid Mediated Transfection of pEGFP-c1 and pJ-EPO Plasmids in Chinese Hamster Ovary (CHO) Cells Attached Culture for Transient and Stable Recombinant Human Erythropoietin (rhEPO) Expression Septisetyani, Endah Puji Kusumawati, Arizah Santoso, Adi cationic lipid CHO cells erythropoietin green fluorescent protein transfection Cationic lipid is one of transfection agents which show high efficiency and low cytotoxicity. The transfection efficiencies can be varied depending upon the type or amount of cationic lipids, the cell line or DNA plasmid being used for transfection. The purpose of this study was to find optimal condition for transfection of CHO-K1 and CHO-S cells with pJ-EPO plasmid (containing human erythropoietin/ hEPO gene) compared with pEGFP-c1 plasmid (containing green fluorescence protein/gfp gene) by cationic lipid Lipofectamin 2000TM (lipofectamin) to generate stable transfectant expressing recombinant human erythropoietin (rhEPO). Optimization was carried out regarding the amount of lipofectamin, DNA concentration, and concentration of antibiotic Geneticin (G418) for selection of stable transfectants. By using standard amount of lipofectamin (10 μl/well) in 6-well plate, highest expression level of green fluorescent protein (GFP) was shown after transfection of CHO-K1 cells with 3 μg/well pEGFP-c1 while highest expression level of rhEPO was observed after transfection of CHO-K1 cells with 6, 8, or 10 μg/well pJ-EPO plasmid. The data also indicated that optimal transfection conditions of CHOK1 and CHO-S cells with pJ-EPO were shown with the use of 4 μg/well DNA in combination with 15 μl lipofectamin. Concentration of G418 used during cells selection also affected the expression where strongest rhEPO expression was shown at 750 ng/μl G418 concentration. Similar to GFP expression profile, rhEPO signal was detected very low during selection process sbased on Western blot data at day 9. Stronger rhEPO signal was observed after day 20 when the stable transfectants have been obtained.   National Research and Innovation Agency (BRIN) 2015-06-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7677 10.14203/ann.bogor.2015.v19.n1.9-16 Annales Bogorienses; Vol. 19 No. 1 (2015): Annales Bogorienses; 9-15 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7677/5861 Copyright (c) 2015 Endah Puji Septisetyani, Arizah Kusumawati, Adi Santoso https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7678 2026-10-19T03:56:46Z annales:ART

In-Silico Cloning and Analysis of Divalent Subunit OMP31-SODc Proteins As A Prophylaxis Vaccine Against Brucella melitensis Infection Wijaya, Sri Kartika Kusumawati, Arizah Wardiana, Andri Rubiyana, Yana Husnaa, Ulfatul Santoso, Adi Brucella melitensis outer membrane proteins OMP31 SODc divalent vaccine structure The urgency to develop a new protein subunit based vaccine candidate against Brucella was provoked by its frequent infection to human and livestock. Since Brucella melitensis is found as the most frequently isolated Brucella species from human, thus the outer membrane of B. melitensis becomes a prominent subcellular localization to search for promising antigen to be developed as vaccine candidate due to its interaction with host cell. Among outer membrane proteins suggested by Vaxign program, OMP31 was found as the most promising candidate. Moreover, analysis on other subcellular localization led our interest to SODc protein, which was expected to support OMP31 in triggering immune response. The OMP31-SODc divalent vaccine candidate was analysed in silico to predict its stable three-dimensional structure, cloning process and expectation on the ease during expression, purification and vaccine delivery to elicit the expected immune response.  National Research and Innovation Agency (BRIN) 2015-06-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7678 10.14203/ann.bogor.2015.v19.n1.17-28 Annales Bogorienses; Vol. 19 No. 1 (2015): Annales Bogorienses; 17-27 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7678/5862 Copyright (c) 2015 Sri Kartika Wijaya, Arizah Kusumawati, Andri Wardiana, Yana Rubiyana, Ulfatul Husnaa, Adi Santoso https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7679 2026-10-19T03:56:46Z annales:ART

In Vitro Induction of Tetraploid Pummelo ’Nambangan’ (Citrus maxima (Burm.) Merr.) by Colchicine Treatment Using Germinated Seed, Shoot Tip and Cotyledonary Node As Explants Wulandari, Dyah Retno Purwito, Agus Susanto, Slamet Husni, Ali Ermayanti, Tri Muji colchicine tetraploid pummelo (Citrus maxima (Burm.) Merr.) flow cytometry stomata Tetraploid citrus are important for interploidal hybridization to create triploid seedless citrus. Colchicine is the most commonly used as antimitotic agent to induce polyploid plants. Tetraploid induction by colchicine in Pummelo ‘Nambangan’ was conducted in vitro using different types of explants. The aim of this research was to induce tetraploid pummelo ‘Nambangan’ by colchicine treatment using germinated seed, shoot tip and cotyledonary node as explants. Tetraploid shoot induction was conducted by soaking germinated seeds, shoot tips and cotyledonary nodes in 0.1 % colchicine for 1, 3 and 5 hours. Regenerant shoots were grown on MS medium and their growth was observed after four weeks in culture. Ploidy level was determined using flow cytometry analysis. Stomata density, length and width of stomatal guard cell were also recorded. The results showed that shoot elongation was inhibited by colchicine treatment. Soaking of shoot tip explants in 0.1 % colchicine for 1 hour resulted in 66.66 % of putative tetraploid shoots. Compared to diploid shoots, tetraploids had lower stomata density but bigger in guard cell size.  National Research and Innovation Agency (BRIN) 2015-06-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7679 10.14203/ann.bogor.2015.v19.n1.29-36 Annales Bogorienses; Vol. 19 No. 1 (2015): Annales Bogorienses; 29-36 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7679/5863 Copyright (c) 2015 Dyah Retno Wulandari, Agus Purwito, Slamet Susanto, Ali Husni, Tri Muji Ermayanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7680 2026-10-19T03:56:46Z annales:ART

Response of Increasing NaCl Concentrations on Growth and Proline Content of Tacca leontopetaloides cultured in vitro Martin, Andri Fadillah Hapsari, Betalini Widhi Ermayanti, Tri Muji Tacca leontopetaloides NaCl salinity stress proline content in vitro The effects of increasing NaCl concentrations on growth and proline content of Tacca leontopetaloides cultured in vitro were investigated. T. leontopetaloides were suspected to have high tolerance against salinity, thus the purpose of this research was to investigate the effect of increasing NaCl concentrations added on growth medium on growth and proline content of T. leontopetaloides grown in vitro. In vitro corms were cultured on MS medium supplemented with NaCl at concentrations of 0, 10, 25, 50, 75, 100, 150 and 200 mM, respectively. After six weeks in culture, shoots height, shoots number, leaves number, fresh weight, as well as their proline content were recorded. The results showed that fresh weight of shoots grown on MS medium supplemented with 10, 25 and 75 mM NaCl was higher compared to the control treatment. Fresh weight decreased when shoots were cultured on MS medium supplemented with NaCl at more than 100 mM. Proline content increased along with the increase of NaCl concentrations. Meanwhile, the height of shoots, number of shoots, and number of leaves decreased along with the increase of NaCl concentrations.  National Research and Innovation Agency (BRIN) 2015-06-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7680 10.14203/ann.bogor.2015.v19.n1.37-42 Annales Bogorienses; Vol. 19 No. 1 (2015): Annales Bogorienses; 37-43 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7680/5864 Copyright (c) 2015 Andri Fadillah Martin, Betalini Widhi Hapsari, Tri Muji Ermayanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7681 2026-10-19T03:56:46Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://ejournal.brin.go.id/annales/article/view/7681 10.14203/annalesbogorienses.2015.v19.n2.i Annales Bogorienses; Vol. 19 No. 2 (2015): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7681/5865 Copyright (c) 2015 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7682 2026-10-19T03:56:46Z annales:REV

Roferon-A: A Biologic Product of Human Interferon Alpha 2a Wardiana, Andri Ningrum, Ratih Asmana interferon alpha 2a Roferon-A therapeutic protein and biosimilar Human interferon alpha 2a (hIFNα2a) is a cytokine regulating immune system that has been used in hepatitis and cancer treatments. It has wide biological potency covering antiviral, antiproliferative and immunomodulative activities. This mini review discusses Roferon-A as a prominent commercial product of recombinant hIFNα2a which is produced in bacterial system, Escherichia coli, as therapeutic protein for several diseases, such as chronic viral Hepatitis B, Hepatitis C, melanoma, hairy cell leukemia and renal cell carcinoma. The discussion focuses on the development process with regard to its manufacturing, preclinical and clinical studies, as well as therapeutic efficacy. In addition, we also discuss biosimilar development of hIFNα2a and its potential future developments in the context of enhancing pharmacokinetic profiles. National Research and Innovation Agency (BRIN) 2015-12-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7682 10.14203/ann.bogor.2015.v19.n2.1-11 Annales Bogorienses; Vol. 19 No. 2 (2015): Annales Bogorienses; 1-11 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7682/5866 Copyright (c) 2015 Andri Wardiana, Ratih Asmana Ningrum https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7683 2026-10-19T03:56:46Z annales:ART

Isolation, Identification, and Evaluation of Antimicrobial Activity of Active Compound Produced by Marine Actinomycetes isolate A32 Sunaryanto, Rofiq Marwanta, Edy marine actinomycetes A32 Madumycin II antimicrobial activity The continuation of new antibiotics exploration becomes an important research program in the world for pharmaceutical and agricultural applications. Marine filamentous bacteria such as actinomycetes have been widely used as an important biological tool to generate a variety of new secondary metabolites, such as antibiotic. The aim of this study was to obtain identified active compound and determine its antimicrobial activity. Isolation, identification, and antimicrobial activity assay of active compound produced by marine actinomycetes isolate A32 had been conducted. Production of active compound using isolate actinomycetes A32 was conducted involving glucose, yeast, peptone medium. The fermentation was carried out at 30 ºC for 5 days. The broth of supernatant was extracted using ethyl acetate. Purification of active compound used chromatography column and eluted stepwise with the chloroform and methanol solvents. Antimicrobial activity was monitored using agar disc diffusion, and microbial test was conducted by analyzing the samples diameter of clear zone towards Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 66923, and Candida albican BIOMCC 00122. Results of isolation and purification of active compound produced by actinomycetes isolate A32 show that this compound has a molecular weight of 503.1 g/mol with molecular formula C26H37N3O7. Furthermore, this compound was suspected as Madumycin II after analysis of spectrum using 1HNMR and COSY. The antimicrobial activity assay confirms that this active compound inhibited the growth of Staphylococcus aureus ATCC 25923 and Candida albican BIOMCC 00122. National Research and Innovation Agency (BRIN) 2015-12-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7683 10.14203/ann.bogor.2015.v19.n2.13-19 Annales Bogorienses; Vol. 19 No. 2 (2015): Annales Bogorienses; 13-19 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7683/5867 Copyright (c) 2015 Rofiq Sunaryanto, Edy Marwanta https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7684 2026-10-19T03:56:46Z annales:ART

Antioxidant Capacities of Holothuria Sea Cucumbers Murniasih, Tutik Putra, Masteria Yunovilsa Pangestuti, Ratih Holothurian sea cucumber extract antioxidant capacity Sea cucumbers are a potential source of biologically active metabolites that are widely used for nutraceutical, pharmaceutical and cosmeceutical products. In this study we investigated the antioxidant activity of several extracts of sea cucumbers, those are Holothuria scabra, Holothuria atra, Holothuria leucospilota, and Holothuria excellens collected from Lombok Island. The selected sea cucumber was then separated to get the active fractions and identified for profiling the secondary metabolites. Phytochemical screening reveals the presence of flavonoid, terpenoids, phenol, saponin, alkaloid, anthraquinone, and glycoside in the extracts of Holothuria sea cucumbers. Radical scavenging effects on the DPPH (1,1-diphenyl-2-picryl-hydrazyl) model were evaluated. The previous work reported that the crude extract of H. atra showed the highest potential of antioxidant capacity. H. atra extract was separated by column chromatography on silica gel and eluted with a gradient system of increasing polarity. Fraction 7 of H. atra extract showed significantly inhibited radical scavenging activity (35.3487 %) at concentration 1 mg/ml. The result of Gas Chromatography-Mass Spectrometry analysis of H. atra fraction 2 showed that it has several active antioxidant compounds which are ethyl cetylate (3), hexadecyl-oxirane (5), 3-chloro-4-hydroxybenzoic acid (6), andoleyl alcohol, trifluoroacetate (9). Moreover, the active fraction 7 indicated several compounds, those are cycloicosane (17), pentadecyl trichloroacetate (18), 14b-pregnane (21) and cis-4-yano-2-(2-hydroxycyclohexyl) pyridine. However, these are preliminary findings and further studies are needed to be focus on the purifications of unknown compounds and formulation for nutraceuticals.  National Research and Innovation Agency (BRIN) 2015-12-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7684 10.14203/ann.bogor.2015.v19.n2.21-26 Annales Bogorienses; Vol. 19 No. 2 (2015): Annales Bogorienses; 21-26 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7684/5868 Copyright (c) 2015 Tutik Murniasih, Masteria Yunovilsa Putra, Ratih Pangestuti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7685 2026-10-19T03:56:46Z annales:ART

Improvement of Endoglucanase Activity in Penicillium oxalicum ID10-T065 Mutated by Ultra Violet Irradiation and Ethidium Bromide Caniago, Asnany Mangunwidjaja, Wibowo Nuswantara, Sukma Lisdiyanti, Puspita endoglucanase mutation ultra violet ethidium bromide Penicillium Penicillium sp. is known as a filamentous fungus that produces complete cellulase. This study aims to improve endoglucanase activity of Penicillium oxalicum ID010-T065 by mutation with ultra violet irradiation (with dose of 0.1 J/cm2, 15 cm), ethidium bromide (10 μg/mL, 1 hour) and combination of both mutagens. The endoglucanase activity of all mutants was higher than that of the wild type (1.03 U/mL). Mutant UVEB-42 exposed to combine mutation showed the highest endoglucanase activity (2.76 U/mL) with a 2.70 fold increase. Mutant EB-45 (1.83 U/mL) exposed to ethidium bromide solution showed a 1.8 fold increase. Mutant UV-13 (1.72 U/mL) exposed to UV irradiation for 3 minutes showed a 1.7 fold increase. All mutants have optimum endoglucanase activity at 50 °C. Mutant UVEB-53 showed the highest thermostability by retaining 86 % of endoglucanase activity at 90 °C. The gene analysis of the endoglucanase I gene (eg1) showed 3 bases mutation in mutant UV-13 and UVEB-53 that changed proline to serine. Mutant EB-45 showed 4 mutated bases that changed valine to glysine and proline to serine. Two bases mutation in Mutant UVEB-53 changed proline to serine. Bases mutation which is occured in eg1 gene could influence the enhance of endoglucanase activity in mutant.  National Research and Innovation Agency (BRIN) 2015-12-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7685 10.14203/ann.bogor.2015.v19.n2.27-38 Annales Bogorienses; Vol. 19 No. 2 (2015): Annales Bogorienses; 27-38 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7685/5869 Copyright (c) 2015 Asnany Caniago, Wibowo Mangunwidjaja, Sukma Nuswantara, Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7686 2026-10-19T03:56:46Z annales:ART

Production of Phytase, Amylase and Cellulase by Aspergillus niger, Neurospora crassa, and Rhizophus oryzae on Sargassum and Rice Bran Under Solid State Fermentation Kanti, Atit amylase cellulase phytase rice bran and sargassum The objective of study was to produce amylase, cellulase and phytase on sargassum and rice bran on solid state fermentation using Aspergillus niger, Neurospora crassa, and Rhizophus oryzae. Media for solid state fermentations composed of dried sargassum and rice bran. The effect of particle of sargassum, initial moisture content on phytase, amilase, and cellulase were evaluated. Optimum enzyme activity of phytase, amylase and cellulase were obtained after 4 days fermentation at 30°C, and initial moisture content was adjusted to 60%. The optimum particle size of dried sargassum attaining the highest enzyme activity was 25 mesh. Best formula forenzymes production was at the ratio of 4:6 (w/w) of Sargassum spinosum (SS) and rice bran (RB) respectively. At this formula highest phytase activity was obtained by Aspergillus niger, cellulase by Rhizopus oryzae, and amylase by Neurospora crassa. Media composed of sargassum and rice bran can be used for phytase, amylase and cellulase production.  National Research and Innovation Agency (BRIN) 2015-12-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7686 10.14203/ann.bogor.2015.v19.n2.39-47 Annales Bogorienses; Vol. 19 No. 2 (2015): Annales Bogorienses; 39-48 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7686/5870 Copyright (c) 2015 Atit Kanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7687 2026-10-19T03:56:46Z annales:ART

Isolation and Identification of Antiplasmodial Compound from Methanol Extract of Calophyllum bicolor P. F. Steven Abbas, Jamilah Hanafi, Muhammad Artanti, Nina Sundowo, Andini Minarti, Minarti Asih, Puji Budi Setia Syafrudin, Din Calophyllum bicolor P. F. Steven Plasmodium falciparum antiplasmodial activity coumarin Calophyllum bicolor (Clusiacea) is a big tree from Indonesian rain forest in Palangkaraya, Central Kalimantan. Calophyllum or bintagor is one of many sources of natural bioactive compounds that can be used in the fields of health and pharmaceuticals. The aim of this research was to explore the antiplasmodial activity of methanol extract of Calophyllum bicolor P.F. Steven against Plasmodium falciparum. The methanol extract was purified by colomn chromatography system, hexane – ethyl acetate was used as solvent with increasing polarity. One pure compound was obtained and was elucidated based on the 1H-&13C-NMR and 2D-NMR, [COSY, HMBC and HMQC] data and the isolated compound was identified as xanthone. Methanol extract showed antiplasmodial activity growth inhibition against P. falciparum with IC50 5.2 ppm and the new 5-methoxy trapezifolixanthone compound have maximum inhibition at concentration 0.11 nMol.  National Research and Innovation Agency (BRIN) 2015-12-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7687 10.14203/ann.bogor.2015.v19.n2.49-56 Annales Bogorienses; Vol. 19 No. 2 (2015): Annales Bogorienses; 49-56 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7687/5871 Copyright (c) 2015 Jamilah Abbas, Muhammad Hanafi, Nina Artanti, Andini Sundowo, Minarti Minarti, Puji Budi Setia Asih, Din Syafrudin https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7688 2026-10-19T03:56:46Z annales:ART

Expression of No Affinity Tagged Recombinant Human Interferon Alpha-2a in Methylotrophic Yeast Pichia pastoris Herawati, Neng Wardiana, Andri Ningrum, Ratih Asmana recombinant hIFN
-2a synthetic gene protein expression Pichia pastoris Recombinant human interferon alpha-2a (rhIFN
-2a) has been widely used for clinical therapy as antiviral, anticancer as well as immunomodulator. In this study, the open reading frame (ORF) encoding synthetic hIFN
-2a was constructed to be in framed with N-terminal alpha factor secretion system in methylotrophic yeast Pichia pastoris. This research aimed to construct, express and analyse the non-affinity tagged recombinant human interferon alpha-2a in the methylotrophic yeast P. pastoris. We used pPICZ
B plasmid for cloning and expression vector. The confirmed recombinant plasmid containing the correct DNA sequence of hIFN
-2a was linearized by SacI restriction enzyme, then transformed into P. pastoris genome using electroporation. We screened two multi-copy recombinants in YPDS plates containing Zeocin™. Buffered complex medium containing 0.5 % methanol (BMMY) was used for protein expression for 48 hours in the culture condition. The recombinant protein was purified by blue sepharose affinity chromatography. Analyses of hIFN
-2a protein by SDS-PAGE and Western blot confirmed that protein band in which was observed around 19.2 kDa, was recombinant hIFN
-2a. The quantification of purified rhIFN
-2a using colorimetric binichoninic assay (BCA) informed that the yield was 44 mg/L culture (OD600= 2-3).  National Research and Innovation Agency (BRIN) 2015-12-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7688 10.14203/ann.bogor.2015.v19.n2.57-62 Annales Bogorienses; Vol. 19 No. 2 (2015): Annales Bogorienses; 57-62 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7688/5872 Copyright (c) 2015 Neng Herawati, Andri Wardiana, Ratih Asmana Ningrum https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7689 2026-10-19T03:56:46Z annales:Pre

EDITOR'S PREFACE Rachmawati, Syamsidah National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7689 10.14203/annalesbogorienses.2016.v20.n1.i Annales Bogorienses; Vol. 20 No. 1 (2016): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7689/5873 Copyright (c) 2016 Syamsidah Rachmawati https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7690 2026-10-19T03:56:46Z annales:ART

Massive In Vitro Propagation of Sandalwood Through Friable Embryogenic Callus Supatmi, Supatmi Ardiyanti, Nurdiya Rahman, Nurhamidar Sudarmonowati, Enny Santalum album somatic embryos friable embryogenic callus plantlets Sandalwood (Santalum album), which belongs to Santalaceae family, is a commercially important tree in Indonesia due to its essential oil contents used for important essence of perfume in the perfumery industry. However, its population has significantly depleted since the planting materials of this tree using conventional methods are difficult to be provided. This study was conducted to mass propagate sandalwood using in vitro methods through friable embryogenic callus (FEC). The somatic embryos were formed using leaves in length of 1-3 and 4-7 mm cultured in MS medium containing 0.5 mg/l and 1 mg/l indole acetic acid (IAA), MS medium supplemented with 1 mg/l IAA and 0.2 mg/l kinetin and half concentration of MS medium supplemented with 1 mg/l Gibberellic acid (GA3). Primary somatic embryos (PSE) and secondary somatic embryos (SSE) then formed friable embryogenic callus when it repetitively transferred into MS medium supplemented with 1.7 mg/l BAP, 1 mg/l proline or 1.5 mg/l BAP and 1.2 mg/l kinetin every 3 weeks. The FEC shows its optimum maturation and regeneration in the MS medium supplemented with 1.5 mg/l BAP and 1.2 mg/l kinetin for 4-8 weeks. The acclimatization of sandalwood plantlets were perfectly conducted in the medium containing soil, sand and compos in ratio of 1:1:1 with the companion plant namely Murraya paniculata, (L) Jack which gave the best percentage of survival rate and the lowest percentage of fallen leaves. These findings may improve the massive propagation of sandalwood through FEC as well as a useful material for further genetic improvement of sandalwood by using FEC as material for genetic transformation. National Research and Innovation Agency (BRIN) 2016-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7690 10.14203/ann.bogor.2016.v20.n1.1-11 Annales Bogorienses; Vol. 20 No. 1 (2016): Annales Bogorienses; 1-11 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7690/5874 Copyright (c) 2016 Supatmi Supatmi, Nurdiya Ardiyanti, Nurhamidar Rahman, Enny Sudarmonowati https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7691 2026-10-19T03:56:46Z annales:ART

Isolation and Characterization of Compounds from the Leaves of Pterocarpus indicus Willd and Their Antioxidant Activity Hartati, Sri Angelina, Marissa Meilawati, Lia Dewijanti, Indah Dwiatmi Pterocarpus indicus Willd antioxidant, flavonoid - glycoside ptevon-3-D- glucoside quercetin The flavone glycoside was isolated from ethyl acetate fraction of ethanol extract of leaves Pterocarpus indicus Willd. The isolation was conducted by gravitation column chromatography and eluted successively with hexane, ethyl acetate and methanol by gradient, and purified by sephadex-LH20. The structure was elucidated base on spectroscopy data of NMR (1D and 2D), UV, LC-MS and FT-IR. Antioxidant was evaluated using 2,2-diphenyl-1-picrylhidrazyl (DPPH) radical scavenging. The isolation and identification led a stigmasterol as Compound 1 and a new flavonol-glycoside [(2R)-7-hydroxy-3-(3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yloxy)-2-(3,4,5-trihydroxy phe nyl)chroman-4-one] or ptevon-3-D- glycoside as Compound 2. Antioxidant activity of Compound 2 showed IC50 for 18.53 μmol and blank of quercetin was 7.94 μmol and Vitamin C was 40.25 μmol. These compounds and antioxidant activities are the first time reported from this plant.  National Research and Innovation Agency (BRIN) 2016-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7691 10.14203/ann.bogor.2016.v20.n1.13-18 Refba Annales Bogorienses; Vol. 20 No. 1 (2016): Annales Bogorienses; 13-18 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7691/5875 Copyright (c) 2016 Sri Hartati, Marissa Angelina, Lia Meilawati, Indah Dwiatmi Dewijanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7692 2026-10-19T03:56:46Z annales:ART

Identification of a New Compound as α-Glucosidase Inhibitor from Aspergillus aculeatus Dewi, Rizna Triana Suparman, Asep Mulyani, Hanny Darmawan, Akhmad Lotulung, Puspa Dewi N. Aspergisllus aculeatus
-glucosidase inhibitory activity rubrofusarin Terestrial fungi are of great importance as potential sources of pharmaceutical agent. Aspergillus aculeatus, a fungus isolated from soil sample collected in Indonesia, was cultured in liquid media to investigate a novel compound as inhibitor
-glucosidase. The mycelium extract of A. aculeatus shows potential activity against Saccharomyces cereviseae
-glucosidase and mild activity against mammalian
-glucosidase with IC50 values of 9.57 μg/mL and 470.76 mg/mL, respectively. Enzyme assay-guided fractionation of this extract afforded rubrofusarin (1). Rubrofusarin, a linear naphtho--pyrone, is a natural pigment from Aspergillus sp. Interestingly, compound 1 shows potential inhibitory activity against mammalian
-glucosidase (IC50 of 92.7 μg/mL), but no to S. cereviseae
-glucosidase. The results suggest that A. aculeatus is a promising natural source as a lead compound in the discovery of antidiabetic drug.  National Research and Innovation Agency (BRIN) 2016-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7692 10.14203/ann.bogor.2016.v20.n1.19-23 Annales Bogorienses; Vol. 20 No. 1 (2016): Annales Bogorienses; 19-24 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7692/5876 Copyright (c) 2016 Rizna Triana Dewi, Asep Suparman, Hanny Mulyani, Akhmad Darmawan, Puspa Dewi N. Lotulung https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7693 2026-10-19T03:56:46Z annales:ART

Bioactivities Screening of Indonesian Marine Bacteria Isolated from Sponges Artanti, Nina Maryani, Faiza Mulyani, Hanny Dewi, Rizna Triana Saraswati, Vienna Murniasih, Tutik marine bacteria sponges anti diabetes antioxidant anti bacteria Currently marine bacteria are considered as important source of natural products for drug discovery. The objective of this study is to conduct an in vitro bioactivities (antidiabetic, antioxidant and antibacterial) screening of 9 Indonesian marine bacteria isolated from sponges that belongs to the Research Center for Oceanography, Indonesian Institute of Sciences collections. The marine bacteria were cultured for 2 days in liquid medium containing yeast, peptone and sea salt under shaking condition and extracted with ethyl acetate. Antidiabetic was measured using inhibition of
-glucosidase inhibitory activity method; antioxidant was measured using DPPH free radical scavenging activity method; antibacterial was tested using disc diffusion method. Screening results showed that at sample concentration of 200 μg/mL, there was significant
-glucosidase inhibitory activity detected in the extracts of strain Sp 7.9 (84 % inhibition) and Sp 8.10 (75 % inhibition), however the antioxidant activities of these two strains were low only around 30 % inhibition, antioxidant activities of other strains were very low. Screening for antibacterial activities using 10 μL samples show that extract of strain Sp 8.5 was best for Staphylococcus aureus (14 mm inhibition); Sp 7.9, and Sp 8.5 for Bacillus subtilis (18 mm inhibition); Sp 8.10 for Escherichia coli (10 mm inhibition); Sp 8.9 and Sp 8.10 (10 mm inhibition) for Pseudomonas aeuriginosa. Based on these results marine bacteria strain Sp 7.9 and Sp 8.10 were selected to be used for further studies in the isolation of bioactive that has potential as antidiabetic and antibacterial. Results of molecular identification conducted by InaCC show that identity of both strains based on BLAST Homology using NCBI database were Bacillus thuringiensis.  National Research and Innovation Agency (BRIN) 2016-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7693 10.14203/ann.bogor.2016.v20.n1.25-30 Annales Bogorienses; Vol. 20 No. 1 (2016): Annales Bogorienses; 25-30 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7693/5877 Copyright (c) 2016 Nina Artanti, Faiza Maryani, Hanny Mulyani, Rizna Triana Dewi, Vienna Saraswati, Tutik Murniasih https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7694 2026-10-19T03:56:47Z annales:ART

Constitutive Expression of Candida antarctica Lipase B (CALB) in Pichia pastoris Using pGAPZα Vector Wahyuni, Febriana Dwi Fuad, Asrul Muhamad Suharsono, Suharsono Candida antarctica lipase B (CALB) pGAPZα constitutive expression Pichia pastoris The synthetic gene of CalBsyn was previously constructed to encode Candida antarctica lipase B (CALB). Lipase of CalBsyn gene is slightly different from wild type CALB (CALB-wt) where it has three amino acids substitutions at different positions, i.e. V210I, A281E, and V221D, in order to improve its thermostability and catalytic efficiency. The CalBsyn gene was isolated from pJ912-CalBsyn vector by digestion using XhoI restriction enzyme. The 1136 bp fragment of CalBsyn gene was then ligated to pGAPZα expression vector and transformed into Escherichia coli TOP10F’ to obtain recombinant vector pGAPZα-CalBsyn. The result show that pGAPZα-CalBsyn recombinant vector was successfully transformed into E. coli TOP10F’ with transformation efficiency of 4.11x103 cfu/μg plasmid DNA. The pGAPZα-CalBsyn recombinant plasmid was successfully introduced into Pichia pastoris SMD1168H using electroporation method with transformation efficiency of 1.01x102 cfu/μg DNA. Recombinant protein expression was analyzed in several selected P. pastoris recombinant strains. Qualitative lipase activity assays results show that transformed P. pastoris-produced extracellular recombinant lipase (CALB) showing lipolytic activity; while results of quantitative lipase activity assays show that this Pichia-derived lipase achieved an activity of 6.35 Units/mL within 48 hours. SDS-PAGE analysis confirms the succesfull expression of CALB protein with molecular size was approximately 45 kDa. National Research and Innovation Agency (BRIN) 2016-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7694 10.14203/ann.bogor.2016.v20.n1.31-38 Annales Bogorienses; Vol. 20 No. 1 (2016): Annales Bogorienses; 29-36 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7694/5878 Copyright (c) 2016 Febriana Dwi Wahyuni, Asrul Muhamad Fuad, Suharsono Suharsono https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7695 2026-10-19T03:56:47Z annales:ART

Molecular Identification of Microalgae BTM 11 and its Lectin Isolation, Characterization, and Inhibition Activity Mustopa, Apon Zaenal Isworo, Rhestu Nurilmala, Mala Susilaningsih, Dwi microalgae BTM 11 lectin antibacterial antiviral BTM 11 is unknown species of microalgae which has active compounds that can inhibit viruses. Lectin is a carbohydrate-binding protein that is found in microalgae with antiviral and antibacterial activities. The purpose of this study was to perform identification, isolation, characterization, and assay of lectin inhibitory activity of microalgae BTM 11. The result shows that microalgae BTM 11 has homology with Cyanobacterium (99%) and Geitlerinema sp (98%). Lectin of microalgae BTM 11 has molecular weight of 17 kDa. Lectin protein activity of microalgae BTM 11 was able to inhibit the enzyme activity of RNA helicase hepatitis C by 57.90% and 27.55%. In addition, the protein was able to suppress the activity of Staphylococcus aureus ATCC 6538, E. coli EPEC K.1.1. and Salmonella typhii ATCC 25241. Activitiy of lectin was stable at 30 °C and unaffected by the action of the enzyme. These results indicate that lectin of microalgae BTM 11 could be an alternative to antiviral and antibacterial proteins. National Research and Innovation Agency (BRIN) 2016-12-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7695 10.14203/ann.bogor.2016.v20.n2.47-54 Annales Bogorienses; Vol. 20 No. 2 (2016): Annales Bogorienses; 47-54 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7695/5879 Copyright (c) 2016 Apon Zaenal Mustopa, Rhestu Isworo, Mala Nurilmala, Dwi Susilaningsih https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7696 2026-10-19T03:56:47Z annales:ART

Generation of mCherryBody: an Anti-Transferrin Receptor Antibody Variable Fragment Linked by The Fluorescent Protein mCherry Kusharyoto, Wien Andriani, Dian Handayani, Ira antibody variable fragment fluorescent protein molecular design mCherry transferrin receptor A facile generation of a recombinant antibody fragment with intrinsic fluorescent properties of the monomeric mCherry fluorescent protein is described. The so-called mCherryBody was designed based on the structure model of the variable fragment of anti-transferrin receptor antibody LUCA31 and the X-ray crystallographic structure of the mCherry protein. mCherryBody was constructed to retain optimal spatial geometry between the C- and N-termini of the antibody light-chain (VL) and heavy-chain (VH) by mimicking the domains interface pairing in antibody Fab fragments and incorporation of the mCherry fluorescent protein as a bridging scaffold. The gene encoding the chimeric protein was cloned into the pJExpress414 expression vector, expressed and secreted into the periplasm of Escherichia coli NiCo21(DE3) for assembly and disulphide bond formation. Based on its amino acid sequence, mCherryBody was predicted to have a molecular weight of 51.46 kDa. The modular assembly used in the generation of mCherryBody may permit the interchange of binding sites and of fluorescent proteins to create robust panels of coloured antibody fragments. Thus, the mCherryBody platform facilitates rapid generation of colored single-chain variable fragment (scFv) chimeras that could be used for screening of antibodies against cell surface markers or receptors. National Research and Innovation Agency (BRIN) 2016-12-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7696 10.14203/ann.bogor.2016.v20.n2.55-62 Annales Bogorienses; Vol. 20 No. 2 (2016): Annales Bogorienses; 55-62 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7696/5880 Copyright (c) 2016 Wien Kusharyoto, Dian Andriani, Ira Handayani https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7699 2026-10-19T03:56:47Z annales:ART

Characterization of Zygotic and Nucellar Embryo of Six Indonesian Mango Cultivars Using Molecular Markers Fatimah, Fatimah Husni, Ali Kosmiatin, Mia Karsinah, Karsinah Baroya, Mushlihatun Mangifera indica polyembryony SSR RAPD SCoT SAP One of difficulties in mangoes hybridization is polyembryonic seeds. This phenomenon reduces the chance of recovering true hybrid seedlings. The identification of the zygotic embryo is difficult and the possible approach is by using molecular marker. The objective of this study was to evaluate the utilization of two different marker systems (dominant markers and SSR) to characterize the occurrence of zygotic or nucellar embryo in polyembryony mango cultivars Garifta Merah, Lalijiwo, Manalagi, Madu, Saigon Kuning, and Saigon Merah. The type of embryo was evaluated by comparing the exhibiting amplification patterns, if different from the mother plant considered as zygotic and identified as nucellar if they exhibited the same banding pattern as the mother plant. From both of this marker systems out of the 16 SSR and 16 evaluated dominant primers, nine primers of each systems amplified the largest number of allele and sharply defined band. Dendogram analysis showed that the evaluated dominant markers could distinguish the zygotic and nucellar embryo clearly compare to evaluated SSR primers. The number of zygotic embryos derived from SSR was 64% and dominant markers were 47%. Based on zygotic and nucellar size, number and position, indicating no relationship between the type of embryo and embryo size, number and the position. National Research and Innovation Agency (BRIN) 2016-12-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7699 10.14203/ann.bogor.2016.v20.n2.69-75 Annales Bogorienses; Vol. 20 No. 2 (2016): Annales Bogorienses; 69-77 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7699/5882 Copyright (c) 2016 Fatimah Fatimah, Ali Husni, Mia Kosmiatin, Karsinah Karsinah, Mushlihatun Baroya https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7705 2026-10-19T03:56:47Z annales:Pre

EDITOR'S PREFACE Rachmawati, Syamsidah National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7705 10.14203/annalesbogorienses.2016.v20.n2.i Annales Bogorienses; Vol. 20 No. 2 (2016): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7705/5887 Copyright (c) 2016 Syamsidah Rachmawati https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7708 2026-10-19T03:56:47Z annales:ART

Overproduction and Purification of Soluble Recombinant Human Granulocyte Colony Stimulating Factor in Escherichia coli Using Thioredoxin as Fusion Agustiyanti, Dian Fitria Retnoningrum, Debbie Sofie Rachmawati, Heni Fuad, Asrul Muhamad G-CSF thioredoxin soluble protein E. coli Recombinant human Granulocyte Colony Stimulating Factor (G-CSF) has been produced in a soluble form in Escherichia coli BL21 (DE3) as a fusion protein. The open reading frame of G-CSF was synthetically constructed in previous work and was codon optimized for best expression in E. coli. In this research, the gene was fused to thioredoxin (Trx) at the N-terminal in pET32 vector. The purpose of this research was to optimize the overproduction and purification processes to obtain high yield recombinant protein in soluble form, and to characterize the Trx-G-CSF fusion protein. Overproduction was performed using IPTG induction method for 3 and 6 hours. The protein was purified by Ni-NTA affinity chromatography and separated using gradient concentration of imidazole. The purified protein was then characterized by SDS-PAGE and Western Blot analysis. Further, enterokinase was used to separate G-CSF from the fusion protein. The purified form of G-CSF was subsequently characterized using Western Blot and mass spectrometry using MALDI-TOF. The results showed that the fusion protein was successfully produced in soluble part as much as 48.25% were obtained after 3 hours of induction. The yield of fusion protein was 67.37% from total protein (229.65 mg protein/L culture). The Western Blot analysis showed the G-CSF band at around 18.6 kDa. Mass spectrometry with MALDI-TOF/ TOF revealed that 25.86% of amino acid residue was recognized as part of human G-CSF sequence. National Research and Innovation Agency (BRIN) 2017-06-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7708 10.14203/ann.bogor.2017.v21.n1.1-8 Annales Bogorienses; Vol. 21 No. 1 (2017): Annales Bogorienses; 1-8 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7708/5889 Copyright (c) 2017 Dian Fitria Agustiyanti, Debbie Sofie Retnoningrum, Heni Rachmawati, Asrul Muhamad Fuad https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7710 2026-10-19T03:56:47Z annales:ART

Enterococcus faecium 1.15 Isolated from Bakasam Showed Milk Clotting Activity Putranto, Wendry Setiyadi Suradi, Kusmajadi Chairunnisa, Hartati Mustopa, Apon Zaenal Kusumaningrum, Harsi Dewantari Suhartono, Maggy Thenawidjaja lactic acid bacteria milk clotting activity rennin-like protease Rennin-like protease of many microorganisms behave similarly to chymosin and are potential alternatives of rennet. The lactic acid bacteria with milk clotting activity were isolated from Bakasam, an Indonesian traditional fermented meat. Screening assay was carried out using modified method of skim milk agar and milk clotting activity test, and the isolate was then identified using 16S rRNA. We found 4 isolates that showed MCA of 18-20 SU/mL. Identification using 16S rRNA indicated that the isolate ALG.1.15 was 99% identical with Enterococcus faecium. The isolate potentially produced renin-like protease to subtitute renin from veal. National Research and Innovation Agency (BRIN) 2017-06-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7710 10.14203/ann.bogor.2017.v21.n1.9-14 Annales Bogorienses; Vol. 21 No. 1 (2017): Annales Bogorienses; 9-14 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7710/5892 Copyright (c) 2017 Wendry Setiyadi Putranto, Kusmajadi Suradi, Hartati Chairunnisa, Apon Zaenal Mustopa, Harsi Dewantari Kusumaningrum, Maggy Thenawidjaja Suhartono https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7713 2026-10-19T03:56:47Z annales:ART

Sequential Adaptation in Mammalian CHO-K1 Cells Producing Human Erythropoietin Wisnuwardani, Popi Hadi Septisetyani, Endah Puji Santoso, Adi adaptation CHO-K1 hEPO serum-free media The production of recombinant proteins for clinical applications using mammalian cell technology has become a prevalent system because of its capacity in assembling functional proteins. One of the main problems with CHO-K1 cells is that this cell has to grow in the presence of serum. However, the presence of serum will complicate the downstream step for protein production. Thus, protein produced in media without serum, theoretically, would be easier to purify. Technically, this type of cell can be produced by growing the CHO-K1 cells in serum-free media by using adaptation method in suspension condition. This research showed that through sequential adaptation using conditioned media, the CHO-K1 cell line that produces the human erythropoietin gene (hEPO) was able to grow in suspension culture using serum-free media. Based on Western blot analysis, it showed that the protein (hEPO) was able to be expressed in suspension culture with molecular mass of about 47 kDa. National Research and Innovation Agency (BRIN) 2017-06-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7713 10.14203/ann.bogor.2017.v21.n1.15-20 Annales Bogorienses; Vol. 21 No. 1 (2017): Annales Bogorienses; 15-20 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7713/5894 Copyright (c) 2017 Popi Hadi Wisnuwardani, Endah Puji Septisetyani, Adi Santoso https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7715 2026-10-19T03:56:47Z annales:ART

Glucoamylase Production by Aspergillus awamori KT-11 In Solid State Fermentation Using Cassava Peel as Substrate Perwitasari, Urip Nuryati, Nuryati Melliawati, Ruth Yopi, Yopi glucoamylase cassava peel A. awamori KT-11 solid state fermentation Cassava has long been known as one of the main staple food in Indonesia. Whereas the cassava peel contains starch of approximately 72%, it is still underrated as a carbohydrate source for fermentation.The utilization of cassava peel as a substrate in solid state fermentation potentially replaces rice as a carbon source leading to more cost-effective production. This study aims at producing glucoamylase by means of solid state fermentation using Aspergillus awamori KT-11 and cassava peel as substrate. The study demonstrated that medium composition and drying technique affected the production of glucoamylase. The highest glucoamylase activities were identified when cassava peel and mineral media was used in fermentation, compared to only cassava peel; the combination of cassava peel, mineral, and rice bran; rice media or a mixture of rice, mineral and rice bran. Freeze-dried glucoamylase, furthermore, exhibited higher specific activity in contrast to the oven-dried one, with 452 U/mL and 365 U/mL, respectively. In conclusion, cassava peel plus mineral is a better substrate for glucosamine production by A. awamori KT-11 in solid state fermentation. Besides, powdered glucoamylase had been demonstrated to be capable of hydrolyzing starch-based biomass. National Research and Innovation Agency (BRIN) 2017-06-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7715 10.14203/ann.bogor.2017.v21.n1.21-28 Annales Bogorienses; Vol. 21 No. 1 (2017): Annales Bogorienses; 21-28 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7715/5896 Copyright (c) 2017 Urip Perwitasari, Nuryati Nuryati, Ruth Melliawati, Yopi Yopi https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7717 2026-10-19T03:56:47Z annales:ART

Comparison of Gene Expression Between Two Types of Anti-EGFRvIII ScFv Antibodies Having Different Variable Domain Orders in Escherichia coli Dewi, Kartika Sari Fuad, Asrul Muhamad EGFRvIII scFv variable domain order antibody Escherichia coli Several studies reported that the expression of various kinds of single-chain variable fragment (scFv) antibodies in Escherichia coli are significantly influenced by the order of their variable domains. To date, the effect of the order of variable domains in the expression of scFv antibodies against epidermal growth factor receptor variant III (EGFRvIII) has not been reported. This study aimed to compare the expression between VH-linker-VL and VL-linker-VH domain orders of the anti-EGFRvIII scFv antibodies in E. coli expression system. Recombinant plasmids inserted with DNA encoding scFv proteins were transformed into E. coli NiCo21 (DE3) competent cells and characterized by colony PCR. The expression of scFv proteins was done by using optimum concentration of inducer. Total proteins, soluble periplasmic and cytoplasmic proteins, also extracellular proteins were isolated, subsequently characterized by SDS-PAGE, Slot Blot, and ImageJ software analyses. The antigen-binding activity of both scFvs proteins against EGFRvIII was observed. The results showed that the relative percentage of scFv expression with VH-linker-VL domain order is higher than that of VL-linker-VH in each compartment. Moreover, both of scFvs proteins have antigen-binding activity against EGFRvIII. National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7717 10.14203/ann.bogor.2017.v21.n1.29-37 Annales Bogorienses; Vol. 21 No. 1 (2017): Annales Bogorienses; 29-37 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7717/5897 Copyright (c) 2017 Kartika Sari Dewi, Asrul Muhamad Fuad https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7718 2026-10-19T03:56:47Z annales:Pre

EDITOR'S PREFACE Rachmawati, Syamsidah National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7718 10.14203/annalesbogorienses.2017.v21.n1.i Annales Bogorienses; Vol. 21 No. 1 (2017): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7718/5899 Copyright (c) 2017 Syamsidah Rachmawati https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7719 2026-10-19T03:56:47Z annales:Pre

EDITOR'S PREFACE Rachmawati, Syamsidah National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7719 10.14203/ab.v21i2.319 Annales Bogorienses; Vol. 21 No. 2 (2017): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7719/5900 Copyright (c) 2017 Syamsidah Rachmawati https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7723 2026-10-19T03:56:47Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2021-01-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7723 10.14203/ann.bogor.2020.v24.n2.%p Annales Bogorienses; Vol. 24 No. 2 (2020): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7723/5902 Copyright (c) 2021 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7725 2026-10-19T03:56:47Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2020-08-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7725 10.14203/ann.bogor.2020.v24.n1.%p Annales Bogorienses; Vol. 24 No. 1 (2020): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7725/5903 Copyright (c) 2020 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7730 2026-10-19T03:56:47Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2020-05-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7730 10.14203/ann.bogor.2019.v23.n2.%p Annales Bogorienses; Vol. 23 No. 2 (2019): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7730/5907 Copyright (c) 2020 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7731 2026-10-19T03:56:47Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2019-10-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7731 10.14203/ann.bogor.2019.v23.n1.%p Annales Bogorienses; Vol. 23 No. 1 (2019): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7731/5908 Copyright (c) 2019 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7732 2026-10-19T03:56:47Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2025-09-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7732 10.14203/ab.v22i1.352 Annales Bogorienses; Vol. 22 No. 1 (2018): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7732/5909 Copyright (c) 2018 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7733 2026-10-19T03:56:47Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2018-12-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/7733 10.14203/ann.bogor.2018.v22.n2.%p Annales Bogorienses; Vol. 22 No. 2 (2018): Annales Bogorienses; i 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7733/5910 Copyright (c) 2018 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/7795 2026-10-19T03:56:47Z annales:ART

Construction of Binary Vector With Wound Inducible Promoter for HbsAg Expression: Development of Plant-Based Edible Hepatitis B Vaccine from Indonesian Isolate Suhandono, Sony Giri-Rachman, Ernawati A. Zainuddin, Ima M. Utari, Putri Dwi Supraba, Apsari Estiati, Amy HBsAg VSPaS signal peptide MeEFI promoter Hepatitis B is a serious infectious disease in the third world countries including Indonesia. Vaccination is the most effective way to prevent the spread of the disease; therefore the demand for HBV vaccine is high. In order to produce more vaccine at lower cost, transgenic plant can be chosen to express the vaccine with the above criteria. Several researches were successfully producing transgenic plants expressing HBsAg that formed virus-like particles and induced immune response in human. However, HBsAg expression in transgenic plant needs to be improved especially on gene expression control system. Here, we describe the construction of HBsAg structural gene under the control of wound inducible promoter, MeEF1 promoter from Manihot esculenta Crantz. The HBsAg gene was amplified using PCR from HBV genome isolated from an Indonesian patient. The gene was subsequently fused with VSPaS signal peptide, which targeted the reticulum endoplasm of plant cell. The construct was cloned into binary expression vector for Agrobacterium plant transformation in near future. National Research and Innovation Agency (BRIN) 2007-06-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/7795 10.14203/ann.bogor.2007.v11.n1.7-14 Annales Bogorienses; Vol. 11 No. 1 (2007): Annales Bogorienses; 7-14 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/7795/5942 Copyright (c) 2007 Sony Suhandono, Ernawati A. Giri-Rachman, Ima M. Zainuddin, Putri Dwi Utari, Apsari Supraba, Amy Estiati https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/13625 2026-10-19T03:56:47Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2025-11-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/13625 Annales Bogorienses; Vol. 26 No. 1 (2022): Annales Bogorienses; i-vii 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/13625/10630 Copyright (c) 2022 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/13626 2026-10-19T03:56:47Z annales:REV

Mini Review: GLP-1 Modification, Development, and Improvement Damai, Fedric Intan Purwanto, Gracia Christine Lembong Wardiana, Andri Wisnuwardhani, Popi Hadi Agustiyanti, Dian Fitria Fathurahman, Alfi Taufiq Ningrum, Ratih Asmana DM-2 GLP-1 DPP-4 protein expression optimization strategy Diabetes Mellitus Type 2 (DM-2) is the condition where the body comes to be insensitive or even resistant towards insulin, thus resulting in deficient insulin secretion from beta cells in the pancreas. Compared with the available treatments, Glucagon-like peptide 1 (GLP-1) is considered a natural treatment to cure DM-2 due to its characteristic as an incretin hormone, where one of its functions is to improve insulin secretion and enhance beta-cell glucose sensitivity. However, GLP-1 has a limitation, which is a rapid half-life due to active degradation activities in the body. Therefore, many studies have been conducted to develop and improve the pharmacological activity of GLP-1 through structural modification and yield improvement, which are thoroughly reviewed in this paper. Structural modification of GLP-1 covers amino acid substitutions by referring to the GLP-1 analog, Exendin4, to prevent the dipeptidyl peptidase-4 (DPP-4) degradation activity and protein fusion with an additional chain to extend the half-life during administration. The yield improvement at the overexpression of GLP-1 tandem repeats sequences can increase the transcribed genes’ yield. The studies show that specific amino acid substitutions and IgG heavy chain constant regions or Fc-based fusion genes successfully enhance the pharmacological activities of GLP-1. At the same time, Pichia pastoris expression system managed to yield 219.2 mg/l target protein, where the purified target protein is capable of producing 10× yield of a single GLP analog. Further research could include the utilization of these findings in vitro as a GLP-1 analog-based therapeutics to treat DM-2. National Research and Innovation Agency (BRIN) 2022-06-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/13626 10.14203/ann.bogor.2022.v26.n1.1-12 Annales Bogorienses; Vol. 26 No. 1 (2022): Annales Bogorienses; 1-12 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/13626/10631 Copyright (c) 2022 Fedric Intan Damai, Gracia Christine Lembong Purwanto, Andri Wardiana, Popi Hadi Wisnuwardhani, Dian Fitria Agustiyanti, Alfi Taufiq Fathurahman, Ratih Asmana Ningrum https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/13627 2026-10-19T03:56:47Z annales:ART

Heterologous Expression of Recombinant Human Insulin Glargine (hIG) in Methylotrophic Yeast Pichia pastoris Herawati, Neng Rubiyana, Yana Desriani, Desriani Santoso, Adi insulin glargine diabetes mellitus Pichia pastoris World health organization (WHO) announced that diabetic patients increased significantly yearly worldwide. Consequently, the need for insulin becomes very large. Pichia pastoris, defined as methylotrophic yeast and a well-known expression and protein production host, is widely used for biopharmaceutical-based drug production. This research aims to synthesize human insulin glargine (hIG) protein in yeast P. pastoris. Human insulin glargine is a group of long-acting analogue insulin.We used a synthetic hIG-encoding gene constructed in frame with the truncated α-factor secretion signal in a pD902 expression vector. Recombinant plasmid pD902-hIG was linearized using Sac1 enzyme and transformed into P. pastoris genome. Multicopy clones were selected on YPDS plates containing Zeocin™ with concentrations of 100; 500; 1,000; and 2,000 µg//mL. Analyses using SDS-PAGE, slot blot, and Western blot showed that recombinant hIG protein had been obtained with a molecular weight of approximately 6,000 Daltons. National Research and Innovation Agency (BRIN) 2022-06-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/13627 10.14203/ann.bogor.2022.v26.n1.13-20 Annales Bogorienses; Vol. 26 No. 1 (2022): Annales Bogorienses; 13-20 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/13627/10632 Copyright (c) 2022 Neng Herawati, Yana Rubiyana, Desriani Desriani, Adi Santoso https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/13628 2026-10-19T03:56:47Z annales:ART

A Preliminary Report on The Syntheses of Oligonucleotide Primers in The National Research and Innovation Agency (NRIA) Atikana, Akhirta Prasetyoputri, Anggia Rubiyana, Yana Herawati, Neng Desriani, Desriani Pratiwi, Riyona Desvy Wulandari, Dwi Sukmarini, Linda Kusharyoto, Wien Santoso, Adi Putra, Masteria Yunovilsa Lisdiyanti, Puspita oligonucleotides DNA synthesizer PCR Indonesia A PolyGen DNA Synthesizer is equipment that is used for synthesizing oligonucleotide primers for any amplification targets. Oligonucleotide primers are indispensable components for any Polymerase Chain Reaction (PCR)-based detections. In the present study a number of oligonucleotide primer sets were synthesized to target (1) the  Human Insuline Glargin (HIG) and (2) the Human Erythropoietin (EPO), as well as (3) the RNA-dependent RNA Polymerase (RdRp) and (4) the Nucleocapsid (N) genes of the severe acute respiratory syndrome virus 2 (SARS-CoV-2). A solid-phase oligonucleotide synthesis method was used according to the default protocol of the Polygen’s instrument to synthesize primers at a 40 nmol scale. The synthesized primers in this study were compared to commercially produced primers in their ability to amplify the gene target(s) in PCR and quantitative real-time PCR (qPCR) reactions. The first two sets of primers showed similar results in PCR compared to commercial primers; however, these primers were not tested for qPCR due to sample limitation. In contrast, the primer sets 3 and 4 were not able to produce amplicons in PCR reactions and only the primer set 4 successfully amplified the gene target in qPCR. These results indicate that the crude primers synthesized in this study are promising candidates for molecular detection and diagnostics, but these primers would benefit from further optimization for routine applications. National Research and Innovation Agency (BRIN) 2022-06-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/13628 10.14203/ann.bogor.2022.v26.n1.21-27 Annales Bogorienses; Vol. 26 No. 1 (2022): Annales Bogorienses; 21-27 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/13628/10633 Copyright (c) 2022 Akhirta Atikana, Anggia Prasetyoputri, Yana Rubiyana, Neng Herawati, Desriani Desriani, Riyona Desvy Pratiwi, Dwi Wulandari, Linda Sukmarini, Wien Kusharyoto, Adi Santoso, Masteria Yunovilsa Putra, Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/13629 2026-10-19T03:56:47Z annales:ART

Antimicrobial Activities of Actinobacteria Isolated from Marine and Terrestrial Samples in Lombok Island, Indonesia Ratnakomala, Shanti Krause, Janina Sari, Miranti Nurindah Kusumawardhani, Dinihari Indah Kusmiati, Mia Lisdiyanti, Puspita actinobacteria antimicrobial activity Lombok Island Indonesia During our study on exploring actinobacteria from Indonesian sources, we selected 59 strains of actinobacteria isolated with five different isolation methods from Lombok Island Indonesia. The aim of this research was to isolate and characterize those actinobacteria, and to screen the microbial activities against 5 bacterial tested. Six soil samples from Lombok Island were processed. Totally, 59 isolated were selected. Of these strains, 31 strains showed antibiotic activity. Seventeen strains from 59 strains have been identified by 16S rRNA gene sequencing, and most dominant of the strains belongs to the genus Streptomyces. Three of them were chosen for further experiments due to their broad activity against four of five test strains. From two strains, at least one bioactive substance could be extracted. As only few bioactive substances could be extracted with ethyl acetate from liquid cultures more experiments should be performed on this subject. More solvent extraction should be tested as well as other production media. In conclusion, present study did now reveal bioactive actinobacteria were isolated from soil samples in Lombok Island. Antimicrobial compound has also been detected which is active against a broad range of pathogens. These findings may have an importance to produce potentially new antibiotic substances from actinobacteria indigenous Indonesia. National Research and Innovation Agency (BRIN) 2022-06-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/13629 10.14203/ann.bogor.2022.v26.n1.28-36 Annales Bogorienses; Vol. 26 No. 1 (2022): Annales Bogorienses; 28-36 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/13629/10634 Copyright (c) 2022 Shanti Ratnakomala, Janina Krause, Miranti Nurindah Sari, Dinihari Indah Kusumawardhani, Mia Kusmiati, Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/13631 2025-11-02T03:23:33Z annales:REV

oai:ejournal.brin.go.id:article/13670 2026-10-19T03:56:47Z annales:ART

Biofilm–Antibiotic Resistance Interplay in Chronic Wound Gram-Negative Pathogens: Insights From an Indonesian Tertiary Hospital: - Anjarwati, Dwi Hapsari, Rebriarina Mujahidah, Mujahidah Wahjono, Hendro Kristina, Tri Nur Nuryastuti, Titik Antibiotic resistance; Biofilm; Chronic wounds; Gram-negative bacteria; Tertiary hospital Biofilm-forming bacteria hinder wound healing and increase antibiotic resistance, complicating the diagnosis and treatment of chronic wound infections. This study aims to investigate the antimicrobial resistance profiles of biofilm-forming Gram-negative bacteria isolated from chronic wound infections in patients at an Indonesian tertiary hospital. A total of 75 Gram-negative bacterial isolates from 60 patients with chronic wound infections were identified and tested for antimicrobial susceptibility. Biofilm formation was assessed using the microtiter plate assay. The Spearman correlation test was used to analyze the relationship between antimicrobial resistance and biofilm production. Gram-negative bacteria accounted for 80% of all isolates. Surgical site infections (SSIs) were the most common wound type. Biofilm-forming ability varied: 45% of isolates were weak, 38.3% moderate, and 16.7% strong producers. Escherichia coli was the dominant species, mainly linked to SSIs, showed weak or no biofilm formation. Klebsiella pneumoniae and Pseudomonas aeruginosa were also prevalent. A weak positive correlation was found between biofilm formation and resistance to amikacin, gentamicin, and meropenem. An inverse correlation was observed with ceftazidime, while no correlation was found with aztreonam, cefepime, or ciprofloxacin. As a conclusion, a weak but significant correlation exists between biofilm production and resistance to certain antibiotics in Gram-negative bacteria.    National Research and Innovation Agency (BRIN) 2025-06-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/13670 10.55981/ann.bogor.2025.13670 Annales Bogorienses; Vol. 29 No. 1 (2025): Annales Bogorienses; 11-18 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/13670/13253 Copyright (c) 2025 Dwi Anjarwati, Rebriarina Hapsari, Mujahidah Mujahidah, Hendro Wahjono, Tri Nur Kristina, Titik Nuryastuti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/14306 2026-10-19T03:56:48Z annales:ART

Optimization of Growth Media, Antibiofilm Evaluation and Taxonomic Identification of Symbiotic Bacteria from the Sea Anemone Heteractis magnifica Novianti, Ela Falah, Syamsul Salsabila, Kania biofilm growth optimization Halalkalibacter okhensis marine bacteria secondary metabolites Antibiotic resistance and biofilm-associated infections remain critical global health challenges. Its responsible for up to 65% of infections in humans and have adaptive resistance 10–1000 times higher against conventional antibiotics. Thus, development and finding of new antibiofilm agent is needed. Marine bacteria, particularly symbion of anemone Heteractis magnifica, offer promising yet underexplored sources of bioactive componds as antibiofilm agents. This research aimed to optimize the growth condition, assess the antibiofilm activities and identify potential strain of symbiotic bacteria from H. magnifica in Samalona Island, Indonesia. The growth of two bacterial isolates (CM01 and CM03) were optimized, revealing that CM01 grew best in TSB with 2%NaCl and 2% peptone at 37°C for 48 h incubation, while CM03 exhibited optimal growth in Zobell medium with 2% peptone at 28°C for 48 h. Secondary metabolites produced under these conditions were tested against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus epidermidis, and Staphylococcus aureus. The 50-hour metabolite of CM03 demonstrated the strongest and broadest antibiofilm activity. Due to its antibiofilm potential, strain CM03 was subjected to genomic characterization using 16S rRNA PCR method. Phylogenetic analysis identified the isolate as Halalkalibacter okhensis. These findings indicate that symbiotic bacteria of H. magnifica, particularly H. okhensis CM03, represent promising candidates for the discovery of new antibiofilm agent. National Research and Innovation Agency (BRIN) 2025-06-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/vnd.openxmlformats-officedocument.wordprocessingml.document https://ejournal.brin.go.id/annales/article/view/14306 10.55981/ann.bogor.2025.14306 Annales Bogorienses; Vol. 29 No. 1 (2025): Annales Bogorienses; 19-28 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/14306/13254 Copyright (c) 2025 Ela Novianti, Syamsul Falah, Kania Salsabila https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/14412 2026-10-19T03:56:48Z annales:ART

In Silico Analysis of Turmeric, Tamarind, and Ginger on the Immune System Rahmatika, Dowmy Puspa, Nabila Swarna Zuhri, Ummu Mastna Ningrum, Andriani Noerlita Wibowo, Joko Tri Immune system needs to be in optimum condition. However, autoimmune diseases occur when the immune system mistakenly attacks the body’s cells, resulting in chronic conditions that require long-term treatment. Current anti-inflammatory and immunosuppressive drugs have significant side effects, highlighting the need for safer and more specific therapeutic alternatives. This study aimed to evaluate turmeric, ginger, and tamarind that have been used traditionally for the drink with benefit for immune system. Compounds from these plants were retrieved from the COCONUT 2.0 database and screened using SwissADME. Target prediction was performed with SwissTargetPrediction and STITCH 5.0, while molecular docking of AKT1, PTGS2, and TP53 was carried out using AutoDockTools-1.5.7. The results show that catechin and kaempferol strongly interact with AKT1, moderately with TP53, and weakly with PTGS2. The number of hydrogen bonds and the involvement of polar residues contributed to complex stability, particularly with AKT1. The results indicated that cathecin and kaempferol in these plants might play an important role as immunomodulatory agents through an in silico analysis. These compounds are not the major constituents of the studied plants. Further research is needed to analyze the primary phytochemicals of these plants and validate their biological activities experimentally. National Research and Innovation Agency (BRIN) 2025-06-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/14412 10.55981/ann.bogor.2025.14412 Annales Bogorienses; Vol. 29 No. 1 (2025): Annales Bogorienses; 35-41 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/14412/13256 Copyright (c) 2025 Dowmy Rahmatika, Nabila Swarna Puspa, Ummu Mastna Zuhri, Andriani Noerlita Ningrum, Joko Tri Wibowo https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/14470 2026-10-19T03:56:48Z annales:ART

Caulerpenyne Compounds from Macroalgae Caulerpa sertularioides against MDM2 p53 Protein as an Anticancer Target Sari, Bunga Anggreini Cancer remains a major global health burden, and limitations of current anticancer therapies highlight the need for safer and more effective alternatives. The MDM2–p53 interaction plays a crucial role in tumor progression by negatively regulating p53 tumor suppressor activity, making it an attractive molecular target for anticancer drug development. Marine macroalgae are recognized as promising sources of bioactive secondary metabolites with potential anticancer properties. This study aimed to investigate the potential of caulerpenyne, a sesquiterpenoid compound derived from the macroalgae Caulerpa sertularioides, as an inhibitor of the MDM2–p53 protein complex using an in silico molecular docking approach. Molecular docking was performed using AutoDock Vina, with validation conducted through redocking of the native ligand, yielding an RMSD value of 0.69 Å, indicating a reliable docking protocol. Drug-likeness and pharmacokinetic properties were evaluated using SwissADME, showing that caulerpenyne fulfills Lipinski’s rule of five with favorable ADME characteristics. Docking results demonstrated that caulerpenyne binds to the MDM2–p53 receptor with a binding free energy of −6.2 kcal/mol and an inhibition constant of 28.5 µM. Interaction analysis revealed hydrogen bonding, hydrophobic, and π-interactions with key amino acid residues involved in p53 binding, including HIS75 and TYR79. Although its binding affinity is lower than that of established synthetic inhibitors, caulerpenyne exhibits a comparable interaction pattern with critical residues of the MDM2 binding pocket. These findings suggest that caulerpenyne has potential as a natural lead compound targeting the MDM2–p53 pathway. Further experimental validation through in vitro and in vivo studies is required to confirm its biological efficacy and therapeutic potential. National Research and Innovation Agency (BRIN) 2025-06-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/14470 10.55981/ann.bogor.2025.14470 Annales Bogorienses; Vol. 29 No. 1 (2025): Annales Bogorienses; 29-33 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/14470/13255 Copyright (c) 2025 Bunga Anggreini Sari https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/14703 2026-10-19T03:56:48Z annales:REV

Mini Review: Extraction Methods, Phytochemical Profile, and Pharmacological Potential of the Marine Sponge Stylissa carteri Tirtanirmala, Prisnu Sari, Bunga Anggreini Stylissa carteri is an Indo-Pacific marine sponge recognized as a prolific source of bioactive secondary metabolites relevant to drug discovery. This mini-review integrates current evidence on extraction strategies, phytochemical diversity, and pharmacological activities reported for this species. Across 12 studies, sample preparation commonly involves freezing, freeze-drying, and pulverization, followed by solvent maceration, predominantly using methanol or ethanol, with polarity-guided fractionation to enrich distinct metabolite classes. Identified constituents include bromopyrrole alkaloids, cyclic peptides, oleanane-type saponins, triterpenes, phenolic diterpenes, fatty acids, ceramides, and cerebrosides, revealing notable geographic and methodological influences on chemical profiles. Biological evaluations demonstrate a broad activity spectrum, including anticancer, antibiofilm, antibacterial, antiviral (anti-HIV), antioxidant, and antiplasmodial effects. Bromopyrrole alkaloids show potent antibiofilm and anti-HIV properties, while cyclic peptides and triterpenes display marked cytotoxicity and antiproliferative action, including effects on drug-resistant cancer cell lines. In addition, metabolites from symbiotic bacteria contribute significant antiplasmodial activity, underscoring the holobiont as a pharmacologically valuable system. Collectively, these findings position S. carteri as a versatile reservoir of marine natural products with multi-target therapeutic potential. Nonetheless, heterogeneity in extraction protocols, limited structural standardization, and insufficient mechanistic studies constrain translational progress. Future investigations should prioritize optimized and reproducible extraction workflows, advanced metabolomic characterization, and targeted pharmacological validation to accelerate the development of S. carteri–derived lead compounds. National Research and Innovation Agency (BRIN) 2025-06-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/14703 10.55981/ann.bogor.2025.14703 Annales Bogorienses; Vol. 29 No. 1 (2025): Annales Bogorienses; 1-10 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/14703/13252 Copyright (c) 2025 Prisnu Tirtanirmala, Bunga Anggreini Sari https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/14829 2026-01-03T14:00:28Z annales:Pre

oai:ejournal.brin.go.id:article/14831 2026-10-19T03:56:48Z annales:ART

Lignocellulosic Characterizations of Indonesian Rice and The Expression Analyses of OsCAD2 and OsMYB55/61 Related To Lignin Biosynthesis Widyajayantie, Dwi Ardie, Sintho Wahyuning Purwoko, Bambang Sapta Laksana, Raden Permana Budi Ismayati, Maya Miyamoto, Takuji Umezawa, Toshiaki Nugroho, Satya lignin lignocellulosic OsCAD2 OsMYB55/61 rice Plant cell walls as sources of lignocellulosic biomass are abundant and used in diverse applications. Lignocellulosic contents manipulation can be applied to develop biomass plants. This study aimed to determine the lignocellulosic components, expressions patterns of OsCAD2 and OsMYB55/61 related to lignin biosynthesis, and deposition patterns of lignin in previously determined three low and three high total lignin content rice accessions based on TGA method. The total Klason and acid-soluble lignin showed variations between 18.03-24.53%, consistent with the TGA method. The ash content of the low lignin content (12.50-21.68%) were significantly differed from the high lignin content (24.84-27.91%) accessions. The extractive, α-cellulose, and hemicellulose contents in each accession showed almost similar proportion. Py-GC-MS proved that the S/G ratio and the syringyl contents were directly proportional to the total lignin contents. OsCAD2 expression levels at the heading stages were consistent with their total lignin contents and their lignin histochemical assay results in the vascular bundle and cortical fiber, suggesting its important roles in rice lignin biosynthesis. However, OsMYB55/61 showed inconsistent expression patterns to total lignin contents, which may also upregulate not only lignin biosynthesis but also cellulose biosynthesis. National Research and Innovation Agency (BRIN) 2022-12-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/14831 10.14203/ann.bogor.2022.v26.n2.1-12 Annales Bogorienses; Vol. 26 No. 2 (2022): Annales Bogorienses; 1-12 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/14831/11471 Copyright (c) 2022 Dwi Widyajayantie, Sintho Wahyuning Ardie, Bambang Sapta Purwoko, Raden Permana Budi Laksana, Maya Ismayati, Takuji Miyamoto, Toshiaki Umezawa, Satya Nugroho https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/14832 2026-10-19T03:56:48Z annales:ART

Study of Putative Pathogenesis-Associated Genes of Rhizoctonia solani AG1-IA Causal Agent of Rice Sheath Blight Hamzah, Pratiwi Farhanah, A Rustam, Aswar putative pathogenesis-associated genes Rhizoctonia solani AG1-IA Indonesia Rhizoctonia solani (Rs), the causal agent of rice sheath blight, is a notorious soil-borne disease prevalent in many rice-growing regions including in Indonesia. Although several studies of pathogenesis of Rs have been reported, there have been no reports describing the putative pathogenesis-associated genes of Rs AG1-IA in Indonesia.  Current study stated that there were 3942 genes that were identified unique to Rs  AG1-IA infecting rice. However, information on interaction and the pathogenicity genes of these Rs anastomosis groups during infection of rice is lacking. Therefore, this study is aims to do inceptive detection of putative pathogenesis-associated genes of Rs AG1-IA in Indonesia. Fifty Rs isolates were collected from thirteen districts of three rice producing provinces in Indonesia; DI Yogyakarta, Central Java, and South Sulawesi, and were identified as Rs AG1-IA based on the presence of 137-140 bp AG1-IA specific primers named Rs1F/Rs2R, also based on macroscopic and microscopic observation. Furthermore, the presence of the putative pathogenesis-associated genes were detected using primers Rga1, AG1_AP, AG1_PC and AG1_CFEM that were applied to selected isolates. The presence of a putative pathogenesis-associated genes was detected by the formation of amplicons at 172 bp using AG1_PC and 144 bp using primers AG1_CFEM. The associated bands showed 100% similarity to glycosyltransferase family 2 protein of Rs AG1-IA for AG1_PC primer and clathrin adapter complex small chain domain for AG1_CFEM primer after sequencing and analyzing by BLASTx tools. While primers Rga1 and AG1_PC didn’t show a specific band. Through the present study, pathogenicity genes were found specific to Rs AG1-IA. AG1_PC in MAG B2 and SPG A2 isolates; and AG1_CFEM in SPG A2, KLA D1, MAG B2 and SLE B1 isolates. These two primers can be used to next step study to better understand the molecular aspect of pathogenicity in Rs AG1-IA. National Research and Innovation Agency (BRIN) 2022-12-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/14832 10.14203/ann.bogor.2022.v26.n2.13-21 Annales Bogorienses; Vol. 26 No. 2 (2022): Annales Bogorienses; 13-21 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/14832/11472 Copyright (c) 2022 Pratiwi Hamzah, A Farhanah, Aswar Rustam https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/14834 2026-01-03T14:35:14Z annales:Pre

oai:ejournal.brin.go.id:article/14835 2026-01-03T14:35:14Z annales:ART

oai:ejournal.brin.go.id:article/14836 2026-01-03T14:35:14Z annales:ART

oai:ejournal.brin.go.id:article/14890 2026-10-20T13:39:42Z annales:ART

Effects of Nutrient Sources and Incubation Time on Extract Yield and Antibacterial Activity of Sponge-Associated Streptomyces BTA 1-131 Atikana, Akhirta Sukmarini, Linda Nurlizah, Fania Rohmatussolihat, Rohmatussolihat Ratnakomala, Shanti Sriherfyna, Feronika Heppy Lisdiyanti, Puspita Bioactivity Bioprospecting RSM Streptomyces Indonesia Streptomyces BTA1-131, a sponge-associated actinobacterium from Indonesia, has been reported as a potential producer of bioactive compounds. The bioactivity of BTA1-131 was influenced by the type of growth medium. Therefore, this current study aims to assess the effects of nutrient sources and time of incubation on the yield extract of BTA1-131 and their antibacterial activity against Staphylococcus aureus ATCC 13420. The study employed a Central Composite Design through Response Surface Methodology (RSM) approach, followed by the  selection of six growth medium compositions derived from the RSM strategy, which was subsequently evaluated for their bioactivity using the agar plug method. The results have shown substantial effects of nutrients (yeast, starch and NaCl) and time of incubation, on the yield extract and antibacterial activity of sponge-associated Streptomyces BTA1-131. This finding contributes to marine bioprospecting, particularly in the discovery and development of (new) antimicrobials from potential indigenous strains. National Research and Innovation Agency (BRIN) 2025-12-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/14890 10.55981/ann.bogor.2025.14890 Annales Bogorienses; Vol. 29 No. 2 (2025): Annales Bogorienses; 1-10 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/14890/13277 Copyright (c) 2025 Akhirta Atikana https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/16676 2026-10-19T03:56:48Z annales:Pre

EDITOR'S PREFACE Lisdiyanti, Puspita National Research and Innovation Agency (BRIN) 2022-12-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/annales/article/view/16676 10.14203/annalesbogorienses.2022.v26.n2.i-vii Annales Bogorienses; Vol. 26 No. 2 (2022): Annales Bogorienses; i-vii 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/16676/12764 Copyright (c) 2022 Puspita Lisdiyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/16677 2026-07-30T15:38:05Z annales:ART

oai:ejournal.brin.go.id:article/16715 2026-10-19T03:56:48Z annales:ART

Characterization Bee Bread Associated Microbials from Tetragonula laeviceps and Identification of Bacillus subtilis as Antibacterial Agent Gondowardoyo, Antonia Caitlin Damayanti, Ema Anggraeni, Mastari Ayu Nabila, Tsania Taskia Putra, Noorendra Laksamana Muslimatun, Siti Chaidir , Riri Rimbun Anggih Djaafar, Titiek Farianti Bee Bread Pollen Antibacterial Agent Associated Microbials Stingless Bee Bee bread is a fermented bee product originating from bee pollen carried by bees and stored in pots in the hive, specifically by stingless bees. Bee bread serves as a food reserve for bee colonies and is naturally preserved through a fermentation process involving numerous microorganisms. As a fermented product, bee bread contains beneficial microorganisms that can be used as an antibacterial agents against pathogenic bacteria. This study aimed to isolate anti-pathogenic bacterial microbials from bee bread Tetragonula laeviceps originating from Sumbawa, Indonesia. The isolation of bee bread microbes was performed using different Agar media, including  Chloramphenicol Yeast Glucose Agar (YGCA), Potato Dextrose Agar (PDA), Malt Extract Agar (MEA), and Sabouraud Dextrose Agar (SDA) by using the spread plate plate method. Isolated microbes identification was carried out using Gram staining and catalase activity assay, and antibacterial activity assay was carried out using the well diffusion method on Escherichia coli FNCC 0091, Staphylococcus aureus FNCC 0047, and Pseudomonas aeruginosa FNCC 0070. The isolate with the highest antibacterial activity was isolate 19. Further identification via 16S rRNA gene sequence analysis revealed that isolate 19 shares 94.91% similarity with Bacillus subtilis strain BCRC 10255, a finding also confirmed by morphological characteristics using scanning electron microscopy. This study highlights the potential of B. Subtilis 19, derived from T. laeviceps bee bread, as a promising antibacterial agent. National Research and Innovation Agency (BRIN) 2026-09-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/16715 10.55981/ann.bogor.2026.16715 Annales Bogorienses; Vol. 30 No. 1 (2026): Annales Bogorienses; 1-10 2407-7518 0517-8452 10.55981/ann.bogor.v30i1 eng https://ejournal.brin.go.id/annales/article/view/16715/13251 Copyright (c) 2026 Antonia Caitlin Gondowardoyo, Ema Damayanti, Mastari Ayu Anggraeni, Tsania Taskia Nabila, Noorendra Laksamana Putra, Siti Muslimatun, Riri Rimbun Anggih Chaidir , Titiek Farianti Djaafar https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/16796 2026-10-19T03:56:48Z annales:ART

Effectiveness of Ethanol Extract of Etlingera balikpapanensis Rhizome as an Antibiofilm Against Staphylococcus aureus MRSA In Vitro Hasyrul Hamzah Henra, Henra Hasriani Hasriani Nur Atika Astriani Etlingera balikpapanensis MRSA Antibiofilm in vitro The increasing prevalence of antibiotic resistance, particularly in Methicillin-Resistant Staphylococcus aureus (MRSA), represents a major challenge in the management of infectious diseases due to the ability of this pathogen to form biofilms that significantly enhance antimicrobial tolerance. Biofilm formation contributes to treatment failure and persistent infections, highlighting the need for alternative strategies, including natural antibiofilm agents. This study aimed to evaluate the antibiofilm activity of the ethanolic extract of Etlingera balikpapanensis rhizome against MRSA in vitro, to assess the effect of different extract concentrations on biofilm formation, and to determine the effective concentration for biofilm inhibition. This research employed an in vitro laboratory experimental design. The rhizome was extracted using the maceration method with 96% ethanol, followed by phytochemical screening and antibiofilm evaluation using the microtiter plate assay with crystal violet staining. The extract was tested at concentrations of 10, 20, and 30 mg/mL with incubation periods of 24, 48, and 72 h. The results demonstrated that the ethanolic extract of E. balikpapanensis rhizome contained alkaloids, flavonoids, saponins, triterpenoids, phenolic compounds, and tannins, and exhibited antibiofilm activity against MRSA at all tested concentrations and incubation times. Biofilm inhibition increased in a concentration-dependent manner, with the 30 mg/mL extract showing the highest inhibitory effect and exceeding the MBIC₅₀ threshold. In conclusion, the ethanolic extract of E. balikpapanensis rhizome has strong potential as a natural antibiofilm agent against MRSA, particularly during the early and early maturation stages of biofilm development. National Research and Innovation Agency (BRIN) 2026-09-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/16796 10.55981/ann.bogor.2026.16796 Annales Bogorienses; Vol. 30 No. 1 (2026): Annales Bogorienses; 11-23 2407-7518 0517-8452 10.55981/ann.bogor.v30i1 eng https://ejournal.brin.go.id/annales/article/view/16796/13257 Copyright (c) 2026 Hasyrul Hamzah, Henra Henra, Hasriani Hasriani, Nur Atika Astriani https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/16860 2026-10-19T03:56:48Z annales:ART

Evaluation of Vitamin C Content and Antioxidant Activities of Kombucha Fermented with Different Herbal Substrates Kholifah, Eva Aini, Silva Kurniasih, Safira Janah, Annisa W. Bikri, Muhamad S. Nuraeni, Reni Shobah, Afifah Nur Stiani, Sofi Nurmay Chairani, Farahdina Noviyanto, Fajrin Kombucha Antioxidant Vitamin C content Etlingera elatior flowers Senna alexandrina leaves Kombucha is a prebiotic of non-dairy products. It is made from fermented plant-based infusion using using symbiotic culture of bacteria and yeast (SCOBY). During the fermentation process, yeast metabolize glucose in the substrat transforms into organic acid, vitamins, and microminerals. This transformation effect increases concentration and pharmacological activity. This research aimed to evaluate the antioxidant activity, vitamin concentration, and correlation between the kombucha process and increasing antioxidants of kombucha. The antioxidant activity was analyzed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and vitamin content was analyzed using spectroscopy UV Vis. Kombucha was prepared using five different substrates such as Etlingera elatior flowers, Senna alexandrina leaves, Syzygium malaccense pericarp, Sargassum crassifolium, and Kappaphycus alvarezii. The antioxidant activity, expressed as IC₅₀, was 27.930, 8.769, 25.132, 22.238, and 118.874 µg/mL, respectively, while the vitamin C contents were 7.97%, 6.92%, 1.568%, 1.51%, and 0.364%, respectively. Among the substrates evaluated, S. alexandrina kombucha exhibited the strongest antioxidant activity, as indicated by its lowest IC₅₀ value (8.769 µg/mL). Correlation analysis revealed a strong positive correlation between the antioxidant activity of the initial infusions and that of the resulting kombucha (r = 0.9656), indicating that substrates with higher initial antioxidant activity tended to produce kombucha with greater antioxidant activity. These findings demonstrate that substrate selection plays an important role in determining the antioxidant properties of kombucha and that S. alexandrina represents a promising substrate for producing kombucha with high antioxidant potential. National Research and Innovation Agency (BRIN) 2026-09-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/16860 10.55981/ann.bogor.2026.16860 Annales Bogorienses; Vol. 30 No. 1 (2026): Annales Bogorienses; 24-31 2407-7518 0517-8452 10.55981/ann.bogor.v30i1 eng https://ejournal.brin.go.id/annales/article/view/16860/13258 Copyright (c) 2026 Eva Kholifah, Silva Aini, Safira Kurniasih, Annisa W. Janah, Muhamad S. Bikri, Reni Nuraeni, Afifah Nur Shobah, Sofi Nurmay Stiani, Farahdina Chairani, Fajrin Noviyanto https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/16926 2026-10-19T03:56:49Z annales:ART

Antibacterial and Biofilm Formation Inhibitory Activities of Kratom (Mitragyna speciosa Korth.) Leaf Extract Against Cutibacterium acnes Ramayta Azizah Henra Henra Hasyrul Hamzah Nur Atika Astriani Divatunnisa , Sajida Khairallah Talia, Nita Suhardiansyah Propionibacterium acnes Antibacterial Antibiofilm Kratom leaf Acne Kratom (Mitragyna speciosa Korth.) is a tropical plant native to Southeast Asia and has demonstrated antibacterial activity; however, information regarding its activity against biofilm formation by Cutibacterium acnes remains limited. This study aimed to evaluate the antibacterial and biofilm formation inhibitory activities of ethanolic kratom leaf extract against C. acnes. Antibacterial activity was evaluated using the disc diffusion assay. Biofilm formation inhibition was assessed using a microtiter plate crystal violet assay with optical density measurement at 595 nm. The extract was tested at 1%, 0.5%, 0.25%, and 0.125% (w/v). The 1% extract produced the largest antibacterial inhibition zone among the extract treatments (10.8 mm). In the biofilm assay, the 1% extract produced 70.67% inhibition after 24 h and 58.75% after 48 h. The lowest tested concentration producing at least 50% biofilm inhibition was 0.5% (w/v) at both time points. These findings indicate concentration-related antibacterial and biofilm formation inhibitory activities of kratom leaf extract against C. acnes. The specific active compounds responsible for these effects remain to be identified. National Research and Innovation Agency (BRIN) 2026-09-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/16926 10.55981/ann.bogor.2026.16926 Annales Bogorienses; Vol. 30 No. 1 (2026): Annales Bogorienses; 32-41 2407-7518 0517-8452 10.55981/ann.bogor.v30i1 eng https://ejournal.brin.go.id/annales/article/view/16926/13259 Copyright (c) 2026 Ramayta Azizah, Henra Henra, Hasyrul Hamzah, Nur Atika Astriani, Sajida Khairallah Divatunnisa , Nita Talia, Suhardiansyah https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/16971 2026-10-19T06:28:45Z annales:ART

Phytochemical Characterization and Analgesic Activity of Ethanol Extract of Majapahit Stem Bark (Crescentia cujete L.) in Male Swiss Webster Mice Diyan Martha, Rahma Danar, Danar Sulastri, Sulastri Kumalasari, Nurul Chamidah Crescentia cujete L stem bark ethanol extract analgesic activity hot plate Majapahit (Crescentia cujete L.) is a medicinal plant traditionally used for various therapeutic purposes and known to contain diverse secondary metabolites with potential pharmacological activities. This study aimed to characterize the phytochemical composition and evaluate the analgesic activity of a 70% ethanol extract of majapahit stem bark in male Swiss Webster mice. The stem bark was extracted by maceration using 70% ethanol. The resulting extract was characterized based on loss on drying, moisture content, total ash content, qualitative phytochemical screening, and quantitative determination of selected secondary metabolites by UV–Vis spectrophotometry. Analgesic activity was evaluated using the hot plate method at extract doses of 125, 250, and 375 mg/kg body weight, with 0.5% CMC-Na and mefenamic acid used as the negative and positive controls, respectively. Loss on drying, moisture content, and total ash content of the crude plant material were 2.90%, 9.73%, and 12.51%, respectively, while the extract yield was 3.29%. Phytochemical screening detected alkaloids, flavonoids, saponins, and tannins. UV–Vis analysis yielded measured levels of 3.31 mg/kg flavonoids, 2.40 mg/kg tannins, 1.09 mg/kg alkaloids, and 0.74 mg/kg saponins. The 375 mg/kg dose produced the highest analgesic response among the extract-treated groups, with 45.0% response protection and 98.0% analgesic activity. Statistical analysis indicated significant differences among treatment groups (p < 0.05), although the Tukey test showed no significant difference among the extract-treated groups and the positive control. These findings indicate that the ethanol extract of majapahit stem bark has potential analgesic activity in male Swiss Webster mice. National Research and Innovation Agency (BRIN) 2026-09-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/16971 10.55981/ann.bogor.2026.16971 Annales Bogorienses; Vol. 30 No. 1 (2026): Annales Bogorienses; 42-49 2407-7518 0517-8452 10.55981/ann.bogor.v30i1 eng https://ejournal.brin.go.id/annales/article/view/16971/13260 Copyright (c) 2026 Rahma Diyan Martha, Danar Danar, Sulastri Sulastri, Nurul Chamidah Kumalasari https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/17044 2026-10-20T13:37:57Z annales:ART

Screening of Antibacterial Activity from Bacteria Associated with the Seaweeds Ulva sp. and Chaetomorpha sp. from Gunungkidul Coast, Indonesia Hanafi, Hanafi Damayanti, Ema Ratih, Dwi Poeloengasih, Cresentiana Dewi Riyanti, Riyanti Seaweed Ulva sp. Chaetomorpha sp. antibacterial agents Seaweed is a strategic multi-industry commodity for food, pharmaceuticals, and cosmetics. The multifunctional properties of seaweed are influenced not only by its nutritional and bioactive content but also by microbes associated with it, both on the surface and as endophytes within its tissues, thus forming a functional holobiont unit. These microbes actively affect the metabolism, morphology, and bioactive compound content which determine seaweed quality for food, medicine, and cosmetics. This study explored bacteria associated with seaweed from Gunungkidul Coast, Indonesia, such as Ulva sp. and Chaetomorpha sp. The aim of this research was to obtain bacteria associated with Ulva sp. and Chaetomorpha sp. that possess antibacterial activity against the pathogenic Escherichia coli and Staphylococcus aureus. Bacterial isolation was performed using the pour plate technique on de Man Rogosa Sharpe Agar (MRSA), Potato Dextrose Agar (PDA), Tryptic Soy Agar (TSA), Zobbel Marine Agar (ZMA), International Streptomyces Project-2 (ISP-2), and International Streptomyces Project-4 (ISP-4) media. Based on the number of colonies that grew, TSA medium was the most optimal, yielding 192 and 357 colonies from Ulva sp. and Chaetomorpha sp., respectively. Antibacterial testing showed that isolates U1 and U2 from Ulva sp. produced the largest inhibition zones, namely 12.71 mm and 5.27 mm against E. coli and 2 mm and 3.07 mm against S. aureus. Further testing of the cell-free supernatant (CFS) showed that these isolates were only active in inhibiting the growth of E. coli. Further genomic and metabolomic studies are needed to reveal more of the potential of bacteria from Ulva sp. as antibacterial agents in the food and pharmaceutical fields.. National Research and Innovation Agency (BRIN) 2025-12-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/17044 10.55981/ann.bogor.2025.17044 Annales Bogorienses; Vol. 29 No. 2 (2025): Annales Bogorienses; 27-35 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/17044/13279 Copyright (c) 2025 Hanafi Hanafi, Ema Damayanti, Dwi Ratih, Cresentiana Dewi Poeloengasih, Riyanti Riyanti https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/17341 2026-10-19T03:56:49Z annales:ART

Preservation Effect Of Peg (Polyethylene Glycol) In Sugarcane (Saccharum officinarum) Nxi 1,3 Synthetic Seed Wulan Nursyiam Ningtiyas Parawita Dewanti Bambang Sugiharto Encapsulation Coleoptillar Embryogenic Callus or Apical Bud Synthetic Seed PEG PBZ Encapsulation is a method for embryogenic tissue coating system by Na-alginate which has synthetic seed preservation purpose. To produce sugarcane synthetic seed need Coleoptillar embryogenic callus and apical bud that it is encapsulated by encapsulation media (MS0 + 4% Na-alginat+ 100 mM CaCl2). In this study, PEG (0%, 1%, 2%, 3%) and PBZ (0 ppm, 1 ppm, 2 ppm, 3 ppm) add to media encapsulation which are give desiccation effect (PEG) and Giberellin synthesis blockade system. Both of PEG or PBZ is used to sugarcane synthetic seed growth retardation that it is implied as storage period. The result are show that PEG 2% is the most effective concentration which is added in encapsulation media. Dessiccation effect of PEG 2% can make synthetic seed storage period until 3 weeks, synthetic seed regeneration produce 89,32 planlets and planlets clorophill concentration is 26,03 ????g/mL. PBZ 1 ppm is the most effective concentration to make storage period until 5 weeks, synthetic seed regeneration produce 39 planlets and planlets clorophill concentration is 35,81 ????g/mL. National Research and Innovation Agency (BRIN) 2016-12-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/17341 10.14203/ann.bogor.2016.v20.n2.63-68 Annales Bogorienses; Vol. 20 No. 2 (2016): Annales Bogorienses; 63-68 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/17341/13180 Copyright (c) 2016 Wulan Nursyiam Ningtiyas, Parawita Dewanti, Bambang Sugiharto https://creativecommons.org/licenses/by-sa/4.0

oai:ejournal.brin.go.id:article/17500 2026-10-20T13:37:32Z annales:ART

Molecular Identification and Antibiofilm Potential of Bacteria Isolated from Vanilla planifolia Beans Laksana, Wildan Dwija Mustikaningtyas, Dewi Lisdiyanti, Puspita Vanilla planifolia beans bacterial diversity 16S rRNA gene antibiofilm activity The bacterial profile associated with Vanilla planifolia beans plays an important role in fermentation processes and the production of bioactive compounds, yet its potential as a source of antibiofilm agents remains poorly understood. This study aimed to isolate bacteria from fermented and dried (unfermented) V. planifolia beans using different pH isolation media, characterize their bacterial profiles through molecular identification, and evaluate their antibiofilm potential against two clinically relevant biofilm-forming pathogens. Bacterial strains were isolated on MRS agar adjusted to pH 4 and pH 7, followed by morphological and Gram-staining characterization. Molecular identification was performed using 16S rRNA gene sequencing and BLAST analysis. Antibiofilm activity was assessed using a microtiter plate assay against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923. A total of 38 bacterial isolates were obtained, with fermented vanilla beans yielding more isolates (23 isolates) than dried (unfermented) vanilla beans (15 isolates). The bacterial profile was dominated by members of the genera Bacillus, Staphylococcus, and Liquorilactobacillus. Eight isolates exhibited strong antibiofilm activity, with biofilm inhibition reaching 89.9% against S. aureus and 99.6% against E. coli. Notably, the detection of Liquorilactobacillus hordei in fermented vanilla beans expands the known ecological distribution of this genus beyond conventional fermented beverages. These findings demonstrate that bacteria associated with fermented and dried/unfermented V. planifolia beans represent promising sources of antibiofilm compounds with potential applications in food safety, healthcare, and biotechnology as sustainable alternatives to conventional antimicrobial agents. National Research and Innovation Agency (BRIN) 2025-12-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/annales/article/view/17500 10.55981/ann.bogor.2025.17500 Annales Bogorienses; Vol. 29 No. 2 (2025): Annales Bogorienses; 21-25 2407-7518 0517-8452 eng https://ejournal.brin.go.id/annales/article/view/17500/13280 https://creativecommons.org/licenses/by-sa/4.0