2026-09-23T22:22:25Z https://ejournal.brin.go.id/JBBI/oai

oai:ejournal.brin.go.id:article/1032 2024-01-12T07:17:43Z JBBI:ART

ENVIRONMENTAL ENHANCEMENT INCREASED SPATIAL MEMORY BUT NO SINGNIFICANT EFFECT ON ANXIETY IN RAT WITH PROPYLTHIOURACIL INDUCTION Noor, Zulkhah Puspitasari, Dewi Alfika Adiyani, Oktavia Environmental enrichment PTU induction spatial memory Anxiety This study aims to determine the effect of environmental enrichment on spatial memory and anxiety in Propylthiouracil (PTU)-induced thyroid disorder rats. The design of this study was an experimental post test and controlled group design to analyze differences in spatial memory in rats at the end of the study. The subjects in this study were normal rat pups and Rat with PTU induction. Subjects were divided into 4 groups namely, the control group, the normal group with environmental enrichment, the rat group with PTU induction, and the rat group with PTU induction with environmental enrichment. Each group consisted of 8 rats. At the time the rats were born, the rats were given 0.025% PTU which was mixed in their drink and given to the mother until the rats were born until they were 4 weeks old. Environmental enrichment was carried out for 6 weeks. The spatial memory test in Rat was carried out when the pups were 60 days old. Data analysis used One Way Annova, Kruskal Wallis, Post Hoc Test and Mann-Whitney Test. Enrichment of the environment increased the learning latency time of normal rats by 27.8 ± 1.509 seconds to 9.9 ± 4.434 seconds with memory retention increasing by 6.85% (p <0.05). Environmental enrichment in PTU-induced rats accelerated from 84.5 ± 8.70 to 77 ± 1.383 with memory retention increasing by 7.06% (p <0.05). Environmental enrichment did not significantly affect the behavior and anxiety of normal rats or PTU induced (p>0.05). Environmental enrichment to normal rats and impaired thyroid function can increase spatial memory but has no effect on reducing anxiety. BRIN Publishing 2023-12-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1032 10.55981/jbbi.2023.1032 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 2 (2023); 331-341 2548-611X 2442-2606 10.55981/jbbi.v10i2 eng https://ejournal.brin.go.id/JBBI/article/view/1032/2147 Copyright (c) 2023 Zulkhah Noor, Dewi Alfika Puspitasari, Oktavia Adiyani https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1700 2023-12-07T14:11:39Z JBBI:ART

MUTATION OF LIPASE-PRODUCING BACTERIAL ISOLATE FROM PALM OIL EFFLUENT FOR FAT HYDROLYSIS ON POME suhendar, dadang Layly, Ika Rahmatul Sabbathini, Gabriela Christy Waltam, Deden Rosid Wahjono, Edi Sriherwanto, Catur Haniyya, Haniyya Lipase is applied to hydrolyzethe residual oil or fat in palm oil mill effluent (POME), which serves as a feedstock for biogas production. A bacterial isolate (Bacillus velezensis) exhibiting lipase activity, previously obtained from Malimping, Pandeglang, Banten, Indonesia, underwentsequential treatments of gamma irradiation (1, 2, and 3 kGy), EMS (ethyl methane sulfonate) mutagenesis (0.5% v/v), and NTG (N-methyl N-nitrosoguanidine) (1 mg mL-1). Following each mutation, lipase activities of the mutant colonies were measured and compared to the wild-type strain. The results revealed that all mutated B. velezensiscolonies exhibited lipase activity values (7.78 ± 0.80 –9.05 ± 0.23 U mL-1) that were not significantly different from the wild-type isolate (8.31 ± 0.01 U mL-1), indicating strong bacterial resistance against the mutagenic treatments. The crude lipase preparation effectively hydrolyzed POME, resulting in a reduction of the COD value (from 131,450 ppm to 88,450 ppm) and O&G content (from 41,400 mg L-1to 5,770 mg L-1) within a 72-hour timeframe. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1700 10.55981/jbbi.2023.1700 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 1 (2023); 11-21 2548-611X 2442-2606 10.55981/jbbi.v10i1 eng https://ejournal.brin.go.id/JBBI/article/view/1700/1234 Copyright (c) 2023 Jurnal Bioteknologi dan Biosains Indonesia https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1701 2025-01-20T10:42:30Z JBBI:ART

OPTIMIZATION OF PRIMARY SOMATIC EMBRYOGENESIS INDUCTION OFCOCOA (Theobroma cacaoL.) THROUGH THE ADDITION OF PHENOLICCOMPOUNDSUPPRESSANT MATERIALS Priandani, Putri Oktaviana Pancaningtyas, Sulistyani Ubaidillah, Mohammad Somatic embryogenesis is a propagation technique in which explants form somatic embryos. The percentage of primary somatic embryo formation in cocoa tends to be low due to the significant secretion of phenolic components in flower explants, thereby reducing the conversion rate of explants into embryos. This is indicated by browning that occurs in explants or the culture medium. The purpose of this study was to optimize primary somatic embryogenesis induction in cocoa through the addition of several types of phenolic compound suppressor materials to the culture medium. Staminodia explants were grownon DKW medium with factorial treatment: various phenolic compound suppressing materials (control; 300 mg L-1PVP; 15 mg L-1silver nitrate; 12 mg L-1melatonin; 300 mg L-1ascorbic acid) and cocoa cultivars (MCC-02 and ICCRI-09). The results showed that each material treatment produced a different response in suppressing phenolic compounds in each cultivar. The melatonin treatment resulted in the lowest percentage of browning intensity, which also had the best impact on morphology, callus size, wet weight,and the percentage of live explants. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article https://ejournal.brin.go.id/JBBI/article/view/1701 10.55981/jbbi.2023.1701 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 1 (2023); 22-34 2548-611X 2442-2606 10.55981/jbbi.v10i1 eng https://ejournal.brin.go.id/JBBI/article/view/1701/1840 Copyright (c) 2023 Jurnal Bioteknologi dan Biosains Indonesia https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ojs.ejournal.brin.go.id:article/1702 2023-10-02T13:16:37Z JBBI:ART

oai:ejournal.brin.go.id:article/1703 2025-01-16T00:29:55Z JBBI:ART

SYRINGIC ACID AND PHENAZINE PRODUCED BY AN ENDOPHYTIC Pseudomonas aeruginosa STRAIN G-111-0317 AND THEIR ACTIVITIES AGAINST Ganoderma boninense Rupaedah, Bedah Nurlaila, Nurlaila Lutfia, Anisa Antifungi phenazine Pseudomonas aeruginosa syringicacid Ganoderma boninense Syringic acid and phenazine possess antibiotic and antifungal properties, and have demonstrated effectiveness in inhibiting the colonization of Ganoderma boninense on oil palm plants. Some bacteria, including Pseudomonas aeruginosa strain G-111-0317, are capable of producing syringic acid and phenazine. In this study, the culture extract of this bacterial strain was obtained from healthy oil palm plants growing in G. boninense-infected areas at Oil Plant Plantation Pematang Siantar, North Sumatra. The strain was cultured on Nutrient Broth (NB) medium, and the resulting culture filtrate was extracted using ethyl acetate (EtOAc) and concentrated under vacuum. The putative compounds were identified by LC-MS, employing syringic acid and phenazine as reference standards. Antifungal activity against G. boninense in vitro was observed in the EtOAc extract obtained after 8 hours and 24 hours of fermentation, with the 8-hour extract demonstrating the highest activity. These compounds hold promising potential as active agents in inhibiting basal stem rot disease in oil palm plants. BRIN Publishing 2023-07-02 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1703 10.55981/jbbi.2023.1703 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 1 (2023); 35-42 2548-611X 2442-2606 10.55981/jbbi.v10i1 eng https://ejournal.brin.go.id/JBBI/article/view/1703/1045 Copyright (c) 2023 Jurnal Bioteknologi dan Biosains Indonesia https://creativecommons.org/licenses/by-nc-sa/4.0

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oai:ejournal.brin.go.id:article/1739 2023-12-07T14:11:37Z JBBI:ART

A REVIEW OF NANOCELLULOSE SYNTHESIS METHODS AND ITS APPLICATION Waluyo, Joko Nanocellulose is a type of cellulose that is being widely developed to replace petroleum-based polymers. This material possesses biocompatible properties, is abundant in nature, and is eco-friendly due to its biodegradability, sustainability, and non-toxic nature. Various nanocellulose synthesis methods are employed, including acid hydrolysis, alkaline, mechanical and biological treatments, as well as ionic liquid and deep eutectic solvent methods. The choice of synthesis method greatly influences the particle size and crystallinity of the resulting nanocellulose; hence further investigation is needed to determine the effectiveness of these methods. Nanocellulose finds applications in various fields, such as films, polymers, cosmetics, medical fuels, and energy storage. Among the different nanocellulose synthesis methods, ionic liquid and deep eutectic solvent (DES) methods have environmentally safe waste with better temperature, time, and diameter control compared to other methods. However, the DES method is currently preferred over the ionic liquid method due to the possibility of separating the lignin waste from the solvent. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1739 10.55981/jbbi.2023.1739 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 1 (2023); 128-149 2548-611X 2442-2606 10.55981/jbbi.v10i1 eng https://ejournal.brin.go.id/JBBI/article/view/1739/1838 https://ejournal.brin.go.id/JBBI/article/view/1739/1848 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1740 2023-12-07T14:11:36Z JBBI:ART

ROOT INDUCTION ON THE SHOOTS OF RUBBER TREE (Hevea brasiliensis) CLONE PB 260 THROUGH EX VITRO TECHNIQUE Fauzan, Yusuf Sigit Ahmad Rubber tree (Hevea brasiliensis) is a crucial plantation commodity for Indonesia's national development. However, the country's rubber productivity lags behind that of other rubber-producing nations. To address this, propagating rubber plants from superior clones through ex-vitro techniques becomes essential.. This study aimed to determine the optimal concentrations of IBA and NAA for root induction in rubber tree shoot explants. A factorial complete randomized design was employed, with the first factor being IBA concentration (0, 100, 200, 300, 400, and 500 ppm) and the second factor being NAA concentration (0, 50, 100, and 150 ppm). The results revealed that the percentage of surviving explants after induction with IBA and NAA did not significantly differ. The rooted explants were obtained using the following treatments: IBA 200 ppm and NAA 0 ppm, IBA 200 ppm and NAA 50 ppm, IBA 300 ppm and NAA ppm, and IBA 400 ppm and NAA 0 ppm. Various concentrations of IBA and NAA did not significantly affect root formation in rubber tree shoot explants. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1740 10.55981/jbbi.2023.1740 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 1 (2023); 105-116 2548-611X 2442-2606 10.55981/jbbi.v10i1 eng https://ejournal.brin.go.id/JBBI/article/view/1740/1841 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1741 2023-12-07T14:11:36Z JBBI:ART

MUTATION DETECTION OF MULTIDRUG-RESISTANT TUBERCULOSIS BY RT-PCR METHOD AS THE DIAGNOSTIC TOOL OF MDR-TB Pambudi, Sabar Eight percent of tuberculosis (TB) cases worldwide are resistant to rifampicin, with mutations occurring in the rpoB and katG genes. It is necessary to develop a specific multidrug-resistant (MDR) diagnostic technique using the RT-PCR method in Indonesia to aid in rapid and accurate diagnosis. In-silico testing using SnapGene software resulted in the design of DNA primers for the katG and rpoB genes, plasmids, and specific probes. This study employed a cross-sectional design using 30 non-MDR-TB and MDR-TB samples from RSUD Sitanala, Tangerang Banten, which were tested for amplification of the katG and rpoB genes using Sybr green RT-PCR. Validity testing was conducted using specific probes for the katG and rpoB genes. The amplification results showed that MDR-TB samples and MDR-TB plasmids required a longer time compared to non-MDR-TB samples and non-MDR-TB plasmids. The Quantification Cycle (Cq) value in non-MDR-TB samples was lower than the Cq value in MDR-TB samples. A t-test revealed a significant difference in Cq values of the rpoB and katG genes between MDR-TB and non-MDR-TB patients (p-value < 0.005). These differences in Cq values indicate that the findings of this study can serve as an initial reference for the development of an RT-PCR-based diagnostic kit for MDR-TB. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1741 10.55981/jbbi.2023.1741 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 1 (2023); 117-127 2548-611X 2442-2606 10.55981/jbbi.v10i1 eng https://ejournal.brin.go.id/JBBI/article/view/1741/1839 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1742 2023-12-07T14:11:36Z JBBI:ART

OPTIMIZATION OF INTRANASAL COVID-19 VACCINE FORMULATION WITH Lactococcus lactis pNZ HCR BACTERIA AS VECTOR IN LIQUID AEROSOL PREPARATION Yurina, Valentina Vaccination is an effective method to suppress COVID-19 transmission, but injection-based vaccination is less effective due to its inability to induce mucosal immunity. This study aimed to determine the effects of vaccine formulations on bacteria viability and antigen expression to find the optimal formulation. Three intranasal preparation formulations (F1, F2, and F3) were created with different ingredient compositions, along with a control. Physicochemical tests were conducted on day 0 and day 14 to assess bacterial viability, and antigen expression was evaluated using the western blot method. Formula 2, containing sodium alginate (0.615%), trehalose (4.125%), polyvinyl alcohol (0.1%), and calcium chloride (5%), exhibited the best viability test results, although no significant differences were observed among the groups. The study concluded that variations in composition concentrations could affect bacterial stability, with Formula 2 showing the best results in terms of bacteria viability and antigen expression up to 14 days after formulation. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1742 10.55981/jbbi.2023.1742 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 1 (2023); 97-104 2548-611X 2442-2606 10.55981/jbbi.v10i1 eng https://ejournal.brin.go.id/JBBI/article/view/1742/1842 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1743 2023-12-07T14:11:35Z JBBI:ART

ISOLATION OF BACTERIA AS A BIOREMEDIATION AGENT FOR RECLAMATION OF MERCURY-CONTAMINATED SOILS Idris, Muhammad Nasution, Rizki Amelia Mayasari , Ulfayani Gold mining commonly involves the use of mercury, which generates hazardous and toxic mercury-based waste, leading to soil contamination and adverse effects on human health. Bioremediation has been explored as a potential solution to this issue. This study aimed to identify indigenous microbial species with potential as bioremediation agents, assess their ability to influence chemical properties, and evaluate their effectiveness in reducing mercury content. The research was conducted in five stages: isolation of native bacteria, testing the potential of microorganisms, assessing the ability to reduce mercury, conducting soil tests, and identifying bacterial characteristics through microscopic and biochemical analyses. The results revealed three types of indigenous microbes, namely Pseudomonas, Neisseria, and Klebsiella bacteria, with the highest potential as bioremediation agents. These bacterial isolates were found to enhance the availability of phosphorus in the soil, maintain soil pH, but had no effect on total soil nitrogen. Furthermore, the bacterial isolates exhibited the ability to reduce mercury content after treatment with NA isolates. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1743 10.55981/jbbi.2023.1743 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 1 (2023); 86-96 2548-611X 2442-2606 10.55981/jbbi.v10i1 eng https://ejournal.brin.go.id/JBBI/article/view/1743/1843 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1744 2023-12-07T14:11:35Z JBBI:ART

ENZYMATIC DEGUMMING USING XYLANASE AND PECTINASE TO IMPROVE BRIGHTNESS AND FINENESS QUALITY OF RAMIE FIBER (Boehmeria nivea L.) AS TEXTILE RAW MATERIAL Abidin, Kharis Yohan Nurhayati, Niknik Nandyawati, Dewi Sabbathini , Gabriela Christy Ramie (Boehmeria nivea L.) fiber is an alternative to cotton fiber, and the degumming process is crucial for preparing it as a textile raw material. This study investigates the enzymatic degumming of ramie fiber using a combination of xylanase enzyme from Bacillus halodurans CM1 and commercial pectinase enzyme. The objective is to assess the impact of enzymatic degumming on the physical properties (weight loss, whiteness index, tenacity, elongation, and fineness) of ramie fiber. The degumming process was conducted in a shaker incubator at a temperature of 50 °C, pH 9, and 150 rpm. The degumming treatment involved 3% v/v xylanase, 1% v/v pectinase, 1% v/v surfactant, and 0.05% v/v NaClO2. The results show that the bleaching treatment (S6) resulted in higher fiber weight loss (9.52%), whiteness index (87.87%), tenacity (20.08 g/Tex), and fineness (1.05 denier) compared to the non-bleaching treatment. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1744 10.55981/jbbi.2023.1744 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 1 (2023); 76-85 2548-611X 2442-2606 10.55981/jbbi.v10i1 eng https://ejournal.brin.go.id/JBBI/article/view/1744/1844 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1745 2023-12-07T14:11:35Z JBBI:ART

LIPID PRODUCTION FROM BR. 2.2 OLEAGINOUS FUNGAL ISOLATE USING ACETATE, GLYCEROL, AND MOLASSES AS CARBON SOURCES Alifiyatin, Chikmatul Darmayanti, Salma Firdasi, Wanny Ilmi, Miftahul Microorganisms that accumulate more than 20% of their dry cell weight as lipids are called oleaginous microorganisms. Oleaginous microorganisms can grow well on various carbon sources other than glucose. These non-glucose alternative carbon sources could potentially reduce high biofuel  manufacturing  costs. BR.  2.2  isolate  is an oleaginous fungus that accumulates 0.62 g L-1 lipids using  glucose as a carbon  source.  This study aims to determine the effect of acetate, glycerol, molasses, and C/N ratios on lipid accumulation of the BR.2.2 isolate. The highest lipid produced by the BR. 2.2 isolate using acetate is 0.196 g L-1 at a C/N ratio of 400, 0.229 g L-1 at a C/N ratio of 225 using glycerol, and 1.97 g L-1 at a C/N ratio of 25 using molasses in 144 hours of incubation. The results revealed that the accumulation of lipids increased with the rising acetate and glycerol C/ N ratios and incubation period. Meanwhile, the accumulation of lipids decreased  with increasing molasses C/N ratio. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1745 10.55981/jbbi.2023.1745 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 1 (2023); 68-75 2548-611X 2442-2606 10.55981/jbbi.v10i1 eng https://ejournal.brin.go.id/JBBI/article/view/1745/1845 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1747 2023-12-07T14:11:35Z JBBI:ART

SCREENING AND CHARACTERIZATION OF SOIL FUNGAL ISOLATES FOR INHIBITING THE GROWTH OF Ganoderma boninense Haqqa, Zhafira Amila Rupaedah, Bedah Handayani, Indri Wahid, Abdul Sugianto, Mahmud Ganoderma boninense, a fungus recognized as a causative agent of basal stem rot and upper stem rot, is primarily found in oil palm plantations (Elaeis guineensis Jack.). This study aimed to identify soil fungal isolates with the greatest potential for inhibiting the pathogenic fungus G. boninense. The research employed curative antagonist testing using in vitro dual culture. Fungal isolates demonstrating the highest inhibition percentages were characterized through macroscopic and microscopic observation, and their hemolysis properties were assessed using blood agar media. Soil fungal isolates FA 3.8 and FA 2.8 exhibited the highest inhibition percentages, reaching 91% and 88%, respectively. Based on morphological characterization at both macroscopic and microscopic levels, FA 3.8 displayed similarities to Trichoderma, while FA 2.8 exhibited similarities to Penicillium. Hemolysis testing results on blood agar media indicated that both isolates exhibited gamma hemolysis or non-hemolysis, as they lacked red blood lysis properties. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1747 10.55981/jbbi.2023.1747 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 1 (2023); 59-67 2548-611X 2442-2606 10.55981/jbbi.v10i1 eng https://ejournal.brin.go.id/JBBI/article/view/1747/1846 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1748 2023-12-07T14:11:35Z JBBI:ART

OPTIMIZATION OF ENZYME-MICROWAVE ASSISTED EXTRACTION, CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF POLYSACCHARIDE FROM Ganoderma lucidum Listriyani, Lira Windriawati Hudiyono, Sumi Rudiyono, Rudiyono Zulaeha, Siti Wibisana, Ahmad Enzyme-Microwave Assisted Extraction (EMAE) is a new process for extracting Ganoderma lucidum polysaccharides (GLPs). Cellic® CTec2 was chosen as an enzyme that assists in microwave extraction. The four variables involved in this study were enzyme concentration (%), enzymatic reaction time (minutes), solvent-to-solid ratio (mL/g), and microwave extraction time (minutes). This study showed that the enzyme concentration and solvent-to-solid ratio had a significant effect on the response in the range studied. Yield extraction of polysaccharides from experiments conducted at optimum conditions showed good agreement with the predictions from the model. The EMAE method showed a higher polysaccharide extraction yield than hot water extraction (HWE) method. GLPs from EMAE method had antioxidant activity of 79.47 ± 0.71% (DPPH) and 0.884 ± 0.013 mM Fe2+/L (FRAP), where these values were higher than those of the HWE method. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1748 10.55981/jbbi.2023.1748 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 1 (2023); 43-58 2548-611X 2442-2606 10.55981/jbbi.v10i1 eng https://ejournal.brin.go.id/JBBI/article/view/1748/1847 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1749 2023-12-04T02:18:23Z JBBI:ART

ANTIOXIDANT EFFECT OF MORINGA (Moringa oleifera) LEAF ETHANOL EXTRACT ON SPERMATOGENESIS IN OLD WISTAR RATS (Rattus norvegicus) Widiastini, Luh Putu Infertility is the failure of pregnancy after regular sexual intercourse for 6-12 months without contraception. Infertility in man is primarily caused by damaged sperm production, for example, impairments in the spermatogenesis process, low spermatozoa concentrations, morphological factors, and abnormal sperm motility. The purpose of this study is to ascertain the antioxidant effect of moringa (Moringa oleifera) leaves ethanol extract on spermatogenesis (spermatogonia, spermatocytes, and spermatids counts) in old Wistar rats (Rattus norvegicus). This study took healthy old Wistar rats aged 18-19 months with a body weight of 200-250 g and with no physical disabilities. A total of 36 was Wistar rats divided into two groups, namely the treatment group (supplied with Moringa leaf ethanol extract of 50 mg/kgBW/ and 0.5% Carboxymethyl cellulose (CMC) of 0.5 mL per day) and the control group (only with 0.5% CMC of 0.5 mL per day) for 30 days. The results showed that there was a significant difference in the spermatogonia, spermatocyte, and spermatid counts between the group treated with Moringa leaf ethanol extract and the control group, resulting in a p-value of 0.000. Therefore, it can be concluded that Moringa leaf ethanol extract had a significant influence on the spermatogonia, spermatocyte, and spermatid counts in old Wistar rats. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1749 10.55981/jbbi.2022.1749 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 2 (2022); 150-157 2548-611X 2442-2606 10.55981/jbbi.v9i2 eng https://ejournal.brin.go.id/JBBI/article/view/1749/1851 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1751 2023-12-04T02:18:23Z JBBI:ART

MICROPROPAGATION OF POTATO (Solanum tuberosum L.) cv. GRANOLA IN LIQUID MEDIUM USING AERATION SYSTEM FOR G0 SEED PRODUCTION Karyanti Disease-free potato seeds of high quality can be obtained via in vitro culture. The use of liquid medium during in vitro cultures might boost the number of plantlets produced, however, the problem of hyperhydricity in plantlets was often encountered. This study aimed to investigate effects of different sucrose concentrations and application of aeration system on micropropagation of potato cv. Granola using liquid medium. Aseptic nodal explants with 3-4 nodes from established in vitro cultures were subjected to MS liquid medium with a factorial treatment of three sucrose concentrations (0, 7.5, 15 g. L-1) and two culture  (with and without aeration). The results showed that MS medium with 7.5 g L-1 sucrose was the best medium to produce the highest number of shoots and nodes. Furthermore, it was found that application of aeration system in MS liquid culture decreased plantlet hyperhydricity and increased the number of shoots, number nodes, plantlet height, as well as improved plantlet morphology and vigor. Application of the aeration system in liquid medium produced 200-230 new potato plants per bioreactor in the acclimatization stage and an average of 2773.5 G0 mini tubers. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1751 10.55981/jbbi.2022.1751 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 2 (2022); 158-169 2548-611X 2442-2606 10.55981/jbbi.v9i2 eng https://ejournal.brin.go.id/JBBI/article/view/1751/1850 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1753 2023-12-04T02:18:22Z JBBI:ART

APPLICATION OF RECOMBINANT TRIACYLGLYCEROL LIPASE AND CARBOXYLESTERASE ENZYMES FROM Bacillus velezensis STRAIN S3 FOR POLYESTER SURFACE MODIFICATION Helianti, Is Enzymatic polyester surface modification can be performed with lipase and esterase enzymes. In this study, the polyester fabric modification utilized triacylglycerol lipase (TGA) and carboxylesterase (CES) recombinant enzymes. The effect of these treatments was observed by determining the hydrophilicity level, dye absorption level, hydroxyl group measurement, and fiber surface morphology. The results revealed an elevated hydrophilicity level in polyester fabric, followed by dye absorption improvement and carboxyl group increase. The water absorption times required by the fabric based on the results of TGA, CES, comparative lipase, and negative control treatments were 3±0.05 seconds, 3.5±0.07 seconds, 5±0.05 seconds, and 80±11.54 minutes, respectively. Dye absorption test in polyester fabric based on these groups mentioned above were 52±0.5, 58±0.5, 178±0.5, and 2968±290 seconds. The total hydroxyl group measurement in polyester fabric was observed at 30.9±0.09, 30.5±0.05, 28.6±0.09, and 3 meq/100 g. The SEM observation showed that the enzymatic hydrolysis could alter the porous structure and surface of the fibers. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1753 10.55981/jbbi.2022.1753 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 2 (2022); 170-181 2548-611X 2442-2606 10.55981/jbbi.v9i2 eng https://ejournal.brin.go.id/JBBI/article/view/1753/1849 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1754 2023-12-04T02:18:21Z JBBI:ART

TOTAL LIGNIN CONTENT AND AGROMORPHOLOGICAL CHARACTER DIVERSITIES OF 30 INDONESIAN RICE (Oryza sativa L.) ACCESSIONS Nugroho, Satya Lignin is one of lignocellulosic components in vascular plants, essential in plant mechanical properties, water transport, and defense against pathogens. Furthemore, lignin has been applied in various industry. This study aimed to explore variation of lignin content and its-related morphological traits of Indonesian rice accessions, providing beneficial information for breeding approaches to improve utilization characteristics of grass biomass. Therefore, the total lignin contents of thirty Indonesian rice accessions using Thioglycolic Acid Lignin (TGAL) method and their correlation to seven agromorphological characters using Pearson correlation analysis were investigated. Variation of lignin content ranged from 8.38 to 20.75% (of cell wal residue, CWR), and the average value was 13.55%. Correlation analysis showed that lignin total positively correlated with plant height, stem length, panicle length, stem diameter, total fresh weight, and panicle weight per tiller. On the other hand, the number of tillers had a significantly negative correlation to lignin contents. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1754 10.55981/jbbi.2022.1754 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 2 (2022); 182-194 2548-611X 2442-2606 10.55981/jbbi.v9i2 eng https://ejournal.brin.go.id/JBBI/article/view/1754/1854 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1755 2023-12-04T02:18:21Z JBBI:ART

OPTIMIZATION OF Bacillus paramycoides FERMENTATION MEDIUM TO INCREASE THE PRODUCTION OF 5-AMINOLEVULINIC ACID IN A 10 LITER FERMENTER Monconegoro, Dicky Adihayyu 5-Aminolevulinic acid is an essential precursor for the biosynthesis of tetrapyrrole compounds, such as chlorophyll and heme. 5-ALA has the potential to be used as a plant growth and antioxidant activity enhancer. 5-ALA can be produced through fermentation by Bacillus paramycoides. This study aimed to optimize B. paramycoides fermentation medium to increase 5-ALA production. The optimization was carried out using response surface method (RSM) experimental design. 5-ALA production in a 10 L fermenter was conducted using an optimized medium and supplemented with MSG as a precursor and wood vinegar as an inhibitor. The results showed that the best medium composition was 27.78 g L-1 molasses; 9.145 g L-1 urea; 8.838 g L-1 NaCl; and 32.07 g L-1 glucose, resulting in 10.749 (log CFU mL-1) and 255.30 µM 5-ALA. 5-ALA production trial in a 10 L fermenter produced 581.82 µM 5-ALA. Medium optimization and precursor-inhibitors addition in the fermentation increased the 5-ALA yield 3.2 times compared to before optimization. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1755 10.55981/jbbi.2022.1755 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 2 (2022); 195-207 2548-611X 2442-2606 10.55981/jbbi.v9i2 eng https://ejournal.brin.go.id/JBBI/article/view/1755/1853 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1756 2025-09-12T10:17:19Z JBBI:ART

oai:ejournal.brin.go.id:article/1757 2023-12-04T02:18:20Z JBBI:ART

ANTIBACTERIAL ACTIVITY OF Chaetoceros calcitrans AGAINST PATHOGEN Staphylococcus aureus AND Staphylococcus epidermidis CAUSING SKIN INFECTION Naully, Patricia Gita The microalgae Chaetoceros calcitrans has potential as a natural antibacterial but is rarely applied to pathogens that cause skin infections such as Staphylococcus aureus and S. epidermidis. The purpose of this study was to determine the optimum concentration of C. calcitrans extract to inhibit the growth of S. aureus and S. epidermidis. The antibacterial activity of C. calcitrans was tested by the Kirby Bauer diffusion method. The results showed that C. calcitrans extract dissolved in DMSO with the concentrations of 5, 10, 15, and 25 mg mL-1 could produce inhibition zones on S. aureus and S. epidermidis. The average diameter of the largest inhibition zone resulted in the concentration of 25 mg mL-1, namely 10.1 ± 0.5 mm in S. aureus and 9.3 ± 0.5 mm in S. epidermidis. It can be concluded that the extract of C. calcitrans has antibacterial activity against bacteria that cause skin infections S. aureus and S. epidermidis with the optimum concentration of 25 mg mL-1. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1757 10.55981/jbbi.2022.1757 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 2 (2022); 208-216 2548-611X 2442-2606 10.55981/jbbi.v9i2 eng https://ejournal.brin.go.id/JBBI/article/view/1757/1852 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1758 2023-12-04T02:18:19Z JBBI:ART

THE EFFECT OF LIQUID TOFU WASTE AND GROWING MEDIA ON THE GROWTH AND YIELD OF LONG BEANS (Vigna sinensis L.) Fauziah, Nindi Long bean is a leguminous plant that has the potential to be developed into productive plants. Tofu liquid waste organic fertilizer and planting media are needed to meet nutrient needs, improve the physical and chemical properties of the soil. This study aims to understand the effect of liquid tofu waste, growing media, and their combined interaction on the growth and yield of long beans. This study used a factorial randomized block design (RBD) with 3 replications, 3 treatments of liquid tofu waste of 0 mL (T0), 150 mL (T1), and 300 mL (T2), and 3 planting media, namely cow manure compost + rice husk charcoal (M1), cow manure compost + cocopeat (M2), and cow manure compost + chopped fern (M3). If they had a significant effect, a Tukey's test at a 5% probability level would be performed. The parameters observed included the chlorophyll a and b, net assimilation rate, relative growth rate, height, number of flowers, pod length, and wet fruit weight of long beans. The results showed that the T1 and M3 treatments significantly affected chlorophyll a and b, height, number of flower, and pod length. The T1 and M2 treatments significantly affected the wet fruit weight of long beans.The T2 and M2 treatments significantly affected the net assimilation rate. Finally, the T2 and M3 treatments significantly affected the relative growth rate. There was no interaction effect found between liquid tofu waste and growing media on all research parameters. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1758 10.55981/jbbi.2022.1758 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 2 (2022); 217-226 2548-611X 2442-2606 10.55981/jbbi.v9i2 eng https://ejournal.brin.go.id/JBBI/article/view/1758/1855 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1759 2023-12-04T02:18:19Z JBBI:ART

MORPHOLOGICAL AND TEXTURAL FEATURE EXTRACTIONS FROM FUNGI IMAGES FOR DEVELOPMENT OF AUTOMATED MORPHOLOGY-BASED FUNGI IDENTIFICATION SYSTEM Pramesti, R. Putri Ayu Due to widely varied microscopic shapes, fungal classification can be performed based on their morphological features. In morphology-based identification process, feature extraction takes an important role to characterize each fungal type. Previous studies used feature extraction of fungal images to detect the presence of fungal. In this study, morphological and textural features were extracted to classify three types of fungi: Aspergillus, Cladosporium and Trichoderma. Geometry and moment were used as morphological features. To perform textural feature extraction, the local binary pattern (LBP) and gray level co-occurrence matrix (GLCM) feature extraction method were used. We compared the implemented feature extraction methods in order to get the best classification result. The result showed that geometrical features has the accuracy of 65%, higher than that of LBP (60%), GLCM (45%), and moment accuracy (55%). This suggested that geometric features is important for fungal classification based on their morphology. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1759 10.55981/jbbi.2022.1759 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 2 (2022); 227-236 2548-611X 2442-2606 10.55981/jbbi.v9i2 eng https://ejournal.brin.go.id/JBBI/article/view/1759/1856 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1760 2025-09-12T10:17:19Z JBBI:ART

oai:ejournal.brin.go.id:article/1763 2025-09-12T10:17:18Z JBBI:ART

oai:ejournal.brin.go.id:article/1764 2025-09-12T10:17:18Z JBBI:ART

oai:ejournal.brin.go.id:article/1765 2025-09-12T10:17:18Z JBBI:ART

oai:ejournal.brin.go.id:article/1766 2025-09-12T10:17:18Z JBBI:ART

oai:ejournal.brin.go.id:article/1769 2023-12-04T02:18:18Z JBBI:ART

EFFECTS OF SEED COATING USING PLANT-BENEFICIAL MICROBES ON THE GERMINATION OF Centrosema pubescens SEEDS Handayani, Indri Centrosema pubescens is a legume plant that is commonly used as animal feed, cover crop, and one of the plants used to reclaim critical land. This study was conducted to increase the germination of C. pubescens seeds by coating the seeds using a coating material enriched with a consortium of microbes consisting of nitrogen-fixing bacteria, phytohormones-producing bacteria, phosphate-solubilizing bacteria, and arbuscular mycorrhizal fungi. Germination test on control, seed coating, and microbial seed coating was carried out using the top of the sand method for 28 days. Observational data were tested using ANOVA statistics and LSD tests. Results of the research showed the moisture content of the seeds was 12.45%, the purity of the seeds was 95.11% and the weight of 1000 seeds was 23.74 g. The germination test denoted that the number of normal germinated seeds in seed coating and microbial seed coating was significantly different from the control. In addition, dead seeds in microbial seed coating treatment had the lowest value and were significantly different from other treatments. These results indicate that microbial seed coating can increase seed germination and considerably reduce seed death due to seed-borne pathogens. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1769 10.55981/jbbi.2022.1769 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 2 (2022); 237-246 2548-611X 2442-2606 10.55981/jbbi.v9i2 eng https://ejournal.brin.go.id/JBBI/article/view/1769/1857 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1770 2023-12-04T02:18:17Z JBBI:ART

PRODUCTION OF HUMAN INSULIN PRECURSOR IN A 10 LITER BIOREACTOR USING A METHANOL PULSE FEEDING Puspitasari, Dian Japany Indonesia as a country that had 19.5 million diabetic patients in 2021 is still importing raw materials of human insulin. The development of human insulin production process is needed to support the government’s objective to be independent in medicine aspect. Human insulin precursor (HIP) expressed from Pichia pastoris X33/pD902-IP had been developed and optimized in small-scale cultivation. However, the scaling up in bioreactor 10 L has not been studied. Using a 10 L-bioreactor the fermentation of P. pastoris X33/pD902-IP was developed. Fermentation was done in 120 hours using a basal salt medium (half concentration) for the vegetative and induction media. To induce HIP expression, methanol is fed by pulse strategy with a gradient concentration of 1-3% (v/v) for 48 hours. The dry cell weight (DCW) and HIP titers were 72 g/L and 286 mg/L, respectively. This development is the first fermentation of HIP expressed by P. pastoris X33/pD902-IP in a 10 L-bioreactor. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1770 10.55981/jbbi.2022.1770 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 2 (2022); 247-256 2548-611X 2442-2606 10.55981/jbbi.v9i2 eng https://ejournal.brin.go.id/JBBI/article/view/1770/1858 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1771 2023-12-04T02:18:17Z JBBI:ART

VOLATILE COMPOUND ANALYSIS OF AROMATIC RICE MUTANT LINES USING HS-SPME/GC-MS Riadi, Muh Volatile compound is one of the key factors for aromatic components of rice. This study aimed to identify the key aroma components and their relationship with plant productivity in the Pare Bau variety mutant lines and its wild type. Volatile extraction was carried out using the headspace solid-phase microextraction (HS-SPME) method and analysed by the Gas Chromatography-Mass Spectroscopy (GC-MS) instrument. The results of the identification of volatile compounds showed a total of 224 compounds in the mutant lines and wild type. However, only 14 compounds were suspected as key aroma compounds in Pare Bau rice, namely 2-Acetyl-1-pyrroline, Indole, 1-Octanol, 1-Octen-3-ol, 2,4-Nonadienal, (E,E)-, Octanal, 2-Nonenal, (E)-, 2-Octenal, (E)-, Decanal, Hexanal, Nonanal, Furan, 2-pentyl-, toluene and vanillin. The results on aroma compounds of mutant lines using principal component analysis showed that there were differences in the main characteristics of several strains and wild type. There was no relationship between increasing volatile concentration and plant productivity, vice versa. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1771 10.55981/jbbi.2022.1771 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 2 (2022); 257-267 2548-611X 2442-2606 10.55981/jbbi.v9i2 eng https://ejournal.brin.go.id/JBBI/article/view/1771/1859 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1772 2023-12-04T02:18:16Z JBBI:ART

UTILIZATION OF SAGO DREGS AS RUMINANT FEED BY USING THE FERMENTATION METHOD: LITERATURE REVIEW Susanti, Titi Lahanda Every year there is a change in the stock of ruminant feed that occurs in the rainy season and water shortages in the dry season. Utilization of agricultural waste as an alternative feed is one way to overcome these problems. One of the wastes that have the potential to be used as feed ingredients is sago waste. Sago pulp is a waste that is rich in lignocellulose, namely cellulose. Several biotechnology applications in ruminant feed fermentation can improve properties such as taste, aroma, shelf life, texture and nutritional value of food. Fermentation using mold or yeast, as well as bacteria or a mixture of various microorganisms can increase the nutrients in the feed needed by ruminant feed. Processing of lignocellulosic materials is required to obtain optimal degradation results. The degradation process will convert lignocellulosic material into raw materials that are easily digested by the ruminant. Enzymes produced by microorganisms can increase crude protein, crude fat, carbohydrates, crude fiber, vitamins and minerals. Thus, the fermentation method of sago dregs and its use as feed can increase the nutritional value, so that productivity can be increased when given as feed. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1772 10.55981/jbbi.2022.1772 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 2 (2022); 268-282 2548-611X 2442-2606 10.55981/jbbi.v9i2 eng https://ejournal.brin.go.id/JBBI/article/view/1772/1860 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1773 2023-12-04T02:18:15Z JBBI:ART

REVIEW: ANIMAL SERUM REPLACEMENT IN MESENCHYMAL STEM CELLS CULTURE Noviantari, Ariyani Mesenchymal stem cells (MSCs) are being used in clinical applications and must comply with Good Manufacturing Practices (GMP) standards and The National Agency of Drug and Food Control (NA-DFC)) regulations. MSCs cultured using a culture medium and added with several supplements like animal serums. However, animal serums can be a source of virus transmission. Therefore, it is necessary to substitute supplements for the animal serum that are safe to use in cell therapy using MSCs. The paper discusses substituting animal serum MSCs culture. This paper is a literature review through a literature search in scientific journals and research reports that explains the various studies on free serum in the culture of MSCs. It can be concluded that human platelet lysate (hPL), human platelet-rich plasma (hPRP), human serum (hS), human umbilical cord serum/plasma (hUCS/P), or human plasma-derived supplement for cell culture medium (SCC) can be used as substitutes for animal serum in MSCs culture. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1773 10.55981/jbbi.2022.1773 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 2 (2022); 283-294 2548-611X 2442-2606 10.55981/jbbi.v9i2 eng https://ejournal.brin.go.id/JBBI/article/view/1773/1861 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1775 2023-12-04T02:18:14Z JBBI:ART

INCREASING RECOMBINANT PENICILLIN G ACYLASE PRODUCTION: GENETIC, PROTEIN ENGINEERING, AND PRODUCTIVITY IMPROVEMENT Helianti, Is B-lactam derived antibiotics are the most used globally for treatment against different infections caused by pathogenic bacteria and comprises 65% of the world antibiotics. Recently, penicillin G acylase (PGA) is used as biocatalyst for those B-lactam antibiotics production by which 6-aminopenicillanic acid (6-APA) or 7-aminodeacetoxycephalosporanic acid (7-ADCA) as the building blocks is produced. Commercialized PGA from native microbial resources are still limited to E. coli. Therefore, genetic engineering approach such as cloning and expression in other microbial hosts were assessed to enhance bacterial strains that produce PGA. However, such improvement could increase immature precursors accumulation and lowering the enzyme yield, activity, or stability. This review focus on the review of PGA recombinant produced by several microbial host, their expression levels, and improvement achieved by some modification such as replacement of signal peptide and promoter continued to protein engineering to utilize the enzymes in synthetizing amoxicillin rather than to hydrolyses Penicillin G. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1775 10.55981/jbbi.2022.1775 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 2 (2022); 295-309 2548-611X 2442-2606 10.55981/jbbi.v9i2 eng https://ejournal.brin.go.id/JBBI/article/view/1775/1862 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1777 2025-09-12T10:17:09Z JBBI:EDT

oai:ejournal.brin.go.id:article/1778 2025-09-12T10:17:09Z JBBI:EDT

oai:ejournal.brin.go.id:article/1779 2025-09-12T10:17:09Z JBBI:RVW

oai:ejournal.brin.go.id:article/1781 2023-12-04T02:35:36Z JBBI:ART

INHIBITORY ACTIVITY OF Trichoderma harzianum AGAINST PUTATIVELY PATHOGENIC FUNGUS ON RODENT TUBER (Typhonium flagelliforme) PLANT Sriherwanto, Catur Trichoderma spp. are globally considered as the most dominant biofungicide in the market. Reports on Trichoderma spp. efficacy against pathogenic fungi in commercial crops have been numerous, but much less in medicinal plants. This study aimed at testing the potential biofungicidal activity of Trichoderma harzianum in inhibiting the growth of a putatively pathogenic fungus isolated from rodent tuber (Typhonium flagelliforme) plant. The methods consisted of isolation of fungi from the plant’s surface, soil, and polybags. The isolates were then screened for their putative pathogenicity against rodent tuber before being subjected to 16S rRNA molecular identification and in vitro antagonist test using T. harzianum. Result showed that only isolate K4 showed pathogenicity on T. flagelliforme, and was molecularly identified as Lasiodiplodia theobromae, known globally as fungal pathogen attacking various plants. L. theobromae was inhibited by T. harzianum with inhibition index of 23.0 ± 4.3%, which was about twice higher than that of the positive control nystatin 100.000 IU mL (11.1 ± 0.6%). In conclusion, T. harzianum inhibited the growth of L. theobromae in vitro, hence indicating its biofungicidal potential. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1781 10.55981/jbbi.2022.1781 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 1 (2022); 1-10 2548-611X 2442-2606 10.55981/jbbi.v9i1 eng https://ejournal.brin.go.id/JBBI/article/view/1781/1864 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1782 2023-12-04T02:35:36Z JBBI:ART

ETHANOL EXTRACT OF Moringa oleifera INCREASED THE NUMBER OF SPERMATOZOA AND IMPROVED SPERM MORPHOLOGY OF OLD Rattus norvegicus Widiastini, Luh Putu Aging in men can lead to decreased semen volume, abnormal sperm morphology, and decreased sperm motility. This study aims to determine the effect of ethanol extract of moringa (Moringa oleifera) leaf on the number and morphology of spermatozoa in old Wistar rats (Rattus norvegicus). This study used healthy old rats, 18–19 months old, with a bodyweight of 200–250 g and no physical disabilities. The 36 rats were divided into 2 groups, i.e. the treatment group (fed with ethanol extract of moringa leaves 50 mg/kgBW/0.5 mL CMC 0.5% every day) and the control group (0.5 mL CMC 0.5% every day) for 30 days. The results showed that moringa leaf ethanol extract increased the number of spermatozoa (p-value 0.000) and improved spermatozoa morphology (p-value 0.000). It can be concluded that the ethanol extract of moringa leaves significantly increased the number and improved the morphology of the spermatozoa in the aged rats. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1782 10.55981/jbbi.2022.1782 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 1 (2022); 11-19 2548-611X 2442-2606 10.55981/jbbi.v9i1 eng https://ejournal.brin.go.id/JBBI/article/view/1782/1863 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1783 2023-12-04T02:35:35Z JBBI:ART

SEGREGATION ANALYSIS OF MORPHOLOGICAL CHARACTER AND BIOACTIVE GENOTYPE OF RICE PLANT F3 (CEMPO SALAMET/IR64) POPULATION Ubaidillah, Mohammad Red rice contains high anthocyanin and bioactive antioxidant compounds that prevent free radical reactions. Cempo Salamet has potential as an antioxidant source, and the characteristics are red colored grains, 4–5 months old, 169 cm plant height, 7 productive tillers per plant, and resistance to blast disease. IR64 had been developed with the following characteristics: 3 months old, 85 cm plant height, 20–35 productive tillers per plant, resistance to brown leafhoppers pigment. This study aimed to obtain information on the segregation of the F3 population from crosses between the Cempo Salamet and IR64 varieties. Research methods included preparation and maintenance with genotype analysis. PCR analysis was conducted using SSR markers with primer RM346, RM316, RM228, and RM339. The segregation in F3 plants was 50% for >130 cm plant height, 51% for 10–19 tillers per plant, 67% for 2.2 g/100-grain weight, and 33% strong red for colour intensity. The findings demonstrated that SSR markers RM346, RM339, and RM228 could validate Cempo Salamet, IR64, and F3 DNA bands. However, RM316 could not validate all DNA bands in the research sample. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1783 10.55981/jbbi.2022.1783 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 1 (2022); 20-29 2548-611X 2442-2606 10.55981/jbbi.v9i1 eng https://ejournal.brin.go.id/JBBI/article/view/1783/1867 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1784 2023-12-04T02:35:35Z JBBI:ART

THE APPLICATION OF Fe AND Cr(III) IN GROWING MEDIA AND ITS EFFECT ON PLANT GROWTH AND Cr(III) OXIDATION ON Tagetes erecta Kasmiyati, Sri The oxidation of Cr(III) to Cr(VI) in the environment has a detrimental impact because it can change the form of non-toxic Cr(III) to Cr(VI), which is toxic to organisms. The study aimed to examine the effect of the application of iron (Fe) and trivalent chromium (Cr(III)) compounds in Tagetes erecta growing media on growth and Cr(III) oxidation. Concentrations of Cr(III) 0, 100, and 500 mg/L and Fe 0, 3, 15, and 30 mg/L were applied to the growing media of T. erecta as the model plant. The growth and accumulation of Cr(VI) in plants were measured to determine the effect of Fe and Cr(III) treatment on growth and Cr(III) oxidation. The accumulation of Cr(VI) in the roots and shoots of T. erecta increased significantly due to the addition of Fe in the growing media treated with Cr(III). The highest accumulation of Cr(VI) in the roots and shoots of T. erecta found in the treatment of Cr(III) 500 mg/L and Fe 30 mg/L, were respectively 0.092 g/L and 0.070 g/L. The addition of Fe in growing media containing Cr(III) increased plant height, root length, and shoot dry weight but decreased leaf number and root dry weight. T. erecta root biomass was more affected by the toxic impact of Fe than Cr(III). On the other hand, the inhibition of leaf formation was caused by the toxic effect of Cr(III) rather than Fe. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1784 10.55981/jbbi.2022.1784 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 1 (2022); 30-45 2548-611X 2442-2606 10.55981/jbbi.v9i1 eng https://ejournal.brin.go.id/JBBI/article/view/1784/1866 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1785 2023-12-04T02:35:34Z JBBI:ART

MITOCHONDRIAL DNA DIVERSITY IN FOUR POPULATIONS OF INDONESIAN FRESHWATER GIANT PRAWN (Macrobrachium rosenbergii) Aliah, Ratu Siti Mitochondrial DNA has been widely applied to analyze fish genetic diversity, especially the cytochrome oxidase subunit I (COI) gene, which can be used as a marker of typical variation patterns, both intraspecific and interspecific crossbreeding. Haplotype diversity was observed in four populations of giant freshwater prawns (Macrobrachium rosenbergii) originating from Peureulak River in Aceh, Tabuk River in South Kalimantan, Bengawan Solo River in East Java, and the Siratu strain. Haplotype diversity was observed in the mitochondrial DNA COI gene of 1516 bp and restricted using 5 enzymes, namely AvaII, MspI, RsaI, HaeIII, and TaqI. The diversity of mtDNA COI haplotypes in the population of Peureulak River, Tabuk River, Bengawan Solo River, and Siratu were 0.8183, 0.6222, 0.7461, and 0.8044, respectively. The dendrogram of genetic distance showed that the Bengawan Solo River populations and the Siratu strain were in the same cluster, while Peureulak River and Tabuk River populations were in another group. The four populations can be used as genetic sources in crossbreeding activities based on haplotype data for each population, haplotype diversity, and genetic distance. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1785 10.55981/jbbi.2022.1785 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 1 (2022); 46-56 2548-611X 2442-2606 10.55981/jbbi.v9i1 eng https://ejournal.brin.go.id/JBBI/article/view/1785/1865 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1786 2023-12-04T02:35:34Z JBBI:ART

RESPONSE OF SEED GERMINATION AND GROWTH OF Nepenthes gymnamphora Nees IN VITRO TO THE CONCENTRATION OF MS MINERAL SALT, PEPTONE AND THIDIAZURON Nurchayati, Yulita Nepenthes gymnamphora Nees is a Java's rare endemic species. Ex-situ conservation of this endangered species can be done through in vitro culture technique. The aims of this study were to determine (1) the mineral salt concentration of MS basal media and addition of peptone (P) on N. gymnamphora seed germination and seedling emergence and (2) the effects of TDZ in ½MS medium on seedling growth. Seeds were surface sterilized and cultured on four media formulations (½MS, MS, ½MS+P, MS+P) for 8 weeks. In the second experiment, ten-week-old seedlings, 0,25 cm in length were cultured on ½MS supplemented with 0, 0,5, 1,0, or 1,5 mg/L TDZ. Seedling growth was recorded at 8 weeks of culture. Results of this experiment showed that ½MS was the best medium for N. gymnamphora seed germination as indicated by the highest percentage of germination, the tallest seedling, and the fastest seedling emergence. Moreover, the best growth of N. gymnamphora was found on ½MS without TDZ. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1786 10.55981/jbbi.2022.1786 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 1 (2022); 57-65 2548-611X 2442-2606 10.55981/jbbi.v9i1 eng https://ejournal.brin.go.id/JBBI/article/view/1786/1868 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1787 2023-12-04T02:35:34Z JBBI:ART

MOLECULAR IDENTIFICATION AND MORPHOLOGICAL CHARACTERIZATION OF PATCHOULI (Pogostemon sp.) FROM BATANG REGENCY, CENTRAL JAVA PROVINCE Kusumaningrum, Hermin Pancasakti Patchouli (Pogostemon cablin Benth.) is an essential oil-producing plant from Batang Regency that has excellence in patchouli alcohol contents and highly survives in any condition. Molecular identification has been done in the ITS region because DNA sequences in the ITS rRNA region evolved faster than in other areas. This study aimed to get the molecular and morphological identity of patchouli (Pogostemon sp.) from BPP Kabupaten Batang. The study consisted of sample preparation, DNA isolation, amplification, electrophoresis, sequence data analysis, and phylogenetic analysis using MEGA X. All parts of plant were morphologically identified and compared with patchouli from Sidikalang, Aceh, Java, and China. Extraction DNA produced 301.7 ng ?L–1 concentration and 1.93 purity. Amplification of ITS fragment patchouli produced a 670 bp-sized single band. Phylogenetic analysis showed patchouli BPP related to Pogostemon cablin (KR608752.1) with 98% coverage identity. BPP patchouli showed 62,5% morphological similarity with Sidikalang patchouli compared to Java patchouli. In conclusion, BPP patchouli is a Sidakalang patchouli P. cablin that has undergone environmental adaptation. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1787 10.55981/jbbi.2022.1787 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 1 (2022); 66-74 2548-611X 2442-2606 10.55981/jbbi.v9i1 eng https://ejournal.brin.go.id/JBBI/article/view/1787/1869 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1788 2023-12-04T02:35:33Z JBBI:ART

GENETIC MUTATION INDUCTION OF Monstera adansonii ON VARIOUS MUTAGENS BY DRIPS APPLICATION Haswin, Dwi Wahyuni Monstera adansonii has wide leaves with a split pattern on its strands (split leaf), smooth and shiny to create a basic visualization (background filler) in flower arrangements. This study aims to obtain genetic changes using chemical mutagens. Chemical mutagens used were 250 ppm EMS, 400 and 800 ppm streptomycin, 400 and 800 ppm GA3. The observations were performed after treatments on the parameter of amount of chlorophyll, stomata and leaf color changes. The results showed that both streptomycin treatments significantly reduced the plant height and the number of leaves. The application of streptomycin in high concentration (800 ppm) decreased the total amount of chlorophyll content in the leaves and changed the color as well as the shape of the Monstera leaves. All chemical mutagen treatments had no effect on stomatal density and stomatal area. The changes in leaf color and shape occurred in the high concentration of 800 ppm streptomycin treatment, could not detected by SSR marker. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1788 10.55981/jbbi.2022.1788 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 1 (2022); 75-85 2548-611X 2442-2606 10.55981/jbbi.v9i1 eng https://ejournal.brin.go.id/JBBI/article/view/1788/1870 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1789 2023-12-04T02:35:33Z JBBI:ART

ANTIBACTERIAL ACTIVITY PROFILE OF MANGROVE ENDOPHYTIC FUNGI ISOLATED FROM BERAU REGENCY, INDONESIA Sukarno Mangrove endophytic fungi have the potential to produce secondary metabolites with antibacterial properties. This study aimed to obtain endophytic mold isolates associated with mangrove plants and their antimicrobial activity. Seventeen endophytic molds were isolated from 7 mangrove species originating from Berau, East Kalimantan, Indonesia. Three extracts from the mold isolates OJ-B, OJ-Bu, and 6Xg-Bh showed the highest antibacterial activity with an inhibition zone of 9.63–25.4 mm and with MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) values ??of around 0.625–5 and 2.5–10 mg mL–1, respectively. LC-MS (liquid chromatography-mass spectrometry) analysis identified several compounds with antibacterial potential, namely beauvericin, choline, nicotinic acid, pyridoxine, quinoline, cyclo (phenylalanyl-prolyl), fusarin c, oleamide and borrelidin. The 3 species were molecularly identified as Diaporthe phaseolorum, Fusarium proliferatum and Phomopsis sp. Thus, the endophytic molds from several of the mangrove species produced bioactive compounds as antibacterial. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1789 10.55981/jbbi.2022.1789 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 1 (2022); 86-99 2548-611X 2442-2606 10.55981/jbbi.v9i1 eng https://ejournal.brin.go.id/JBBI/article/view/1789/1871 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1790 2023-12-04T02:35:33Z JBBI:ART

ANALYSIS OF BBM, LEC, AND SERK EXPRESSIONS IN CALLUS OF SUGARCANE (Saccharum officinarum L.) AT SOMATIC EMBRYOGENESIS DEVELOPMENT STAGES Dewanti, Parawita Callus formed in somatic embryogenesis is divided into two types, embryogenic and non-embryogenic callus. Embryogenic callus can be distinguished by the expression of genes as markers related to somatic embryogenesis which are expected to be used as markers to detect callus that has embryogenic capabilities in sugarcane. The aim of this study was to determine the expressions of somatic embryogenesis-related genes in sugarcane. The genes analysis was carried out using somatic embryogenesis callus and using a kit for RNA analysis. Results showed that there were expressions of Baby Boom (BBM), Leafy Cotyledon (LEC), dan Somatic Embryogenesis Receptor Like-Kinase (SERK) gene with specific primer of those three genes, which were collected from embryogenic callus in mass, globular, scutelar, and coleoptilar pre-embryo stages, meanwhile there were no expressions of those genes collected from non-embryogenic callus. Amplification result from PCR product of cDNA using three gene primers detected were in 500 bp for BBM, 400 bp for LEC, and 700 bp for SERK gene. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1790 10.55981/jbbi.2022.1790 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 1 (2022); 100-109 2548-611X 2442-2606 10.55981/jbbi.v9i1 eng https://ejournal.brin.go.id/JBBI/article/view/1790/1872 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1791 2023-12-04T02:35:33Z JBBI:ART

OPTIMIZATION OF Agrobacterium-MEDIATED GENETIC TRANSFORMATION OF oshox4 GENE IN SATOIMO TARO (Colocasia esculenta var. antiquorum) Hartati, N. Sri Satoimo (Colocasia esculenta var. antiquorum) can be considered as an alternative food to support food diversification. The aim of this research was to obtain an optimum condition of the genetic transformation method of Satoimo taro through Agrobacterium-mediated genetic transformation using binary vector harboring gene construct of oshox4 and hpt gene as a selectable marker to produce transgenic plants. Plant materials that were used in this study were meristem, root, leaf, petiole, and basal stem. Transformation procedures were carried out using three different co-cultivation periods (1, 2, and 3 days) and different optical densities of A. tumefaciens cells (0,5 and 0,7). Furthermore, callus induction was performed on MS medium containing 2.4-D, cefotaxime, hygromycin and then regenerated on MS medium containing thidiazuron and hygromycin. The putative transgenic plantlets were selected on MS medium containing hygromycin and analyzed using PCR. The results showed that putative transgenic plants derived from the basal stem could survive on a selection medium containing hygromycin and based on PCR analysis some of them contained the oshox4 and hpt genes. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1791 10.55981/jbbi.2022.1791 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 1 (2022); 110-118 2548-611X 2442-2606 10.55981/jbbi.v9i1 eng https://ejournal.brin.go.id/JBBI/article/view/1791/1873 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1792 2023-12-04T02:35:33Z JBBI:ART

ANTIDIABETIC ACTIVITY OF LEAF EXTRACT OF Clerodenrum fragrans Vent Willd IN Rattus novergicus INDUCED BY ALLOXAN Silaban, Saronom Sarang banua plants are grown in Simalungun, North Sumatra, Indonesia, and have been used by the community as traditional medicinal plants. Sarang banua plant is a type of Clerodendrum fragrans Vent Willd, including the family Verbenaceae. This study aimed to determine the antidiabetic activity of the leaf extract of sarang banua (C. fragrans Vent Willd) in white male rats (Rattus novergicus) induced by alloxan. This study used a RAL design with seven treatments, namely (K0) standard feed, (K1) Na-CMC 0.5%), (K2) metformin, (K3) ethanol extract 100 mg/kg bw, 200 mg/kg bw (K4), 300 mg/kg bw (5), (K6) ethyl acetate extract 200 mg/kg bw and 300 mg/kg bw (K7). Groups K1 to K7 were induced by alloxan before being given treatment. Each treatment was replicated three times. The results showed that the application of leaf extract of the C. fragrans affected on reducing the blood glucose levels of alloxan-induced rats. The used of ethanolic extract of C. fragrans 100 mg/kg bw resulted in the highest percentage decrease in blood glucose (54.46 ± 5.60%) of hyperglycemic rats induced by alloxan, close to a positive control (56.63 ± 1.86%).  BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1792 10.55981/jbbi.2022.1792 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 1 (2022); 119-125 2548-611X 2442-2606 10.55981/jbbi.v9i1 eng https://ejournal.brin.go.id/JBBI/article/view/1792/1874 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1793 2023-12-04T02:35:32Z JBBI:RVW

MICROBIAL BIOCONVERSION TO PRODUCE NUTRACEUTICAL AND PHARMACEUTICAL BIOACTIVE COMPOUNDS Mahyuni, Siti   he bioconversions of bioactive health supporting compounds using certain microorganisms have long been known, however it has not been widely applied in industrial scale to produce nutraceutical and products of high economic value. This review article will discuss the group of steroids, carotenoids and statins which were produced by microbial bioconversion using the various substrates and conditions. The information presented in this article were collected from scientific books and articles in national and international journals which can be validated and traced back to their sources through many sites such as google.com, google scholar, NCBI and Science Direct. From the information compiled in this article, it can be concluded that the bioconversion of bioactive compounds by microorganisms has shown positive results and potential to be further optimized and applied in an industrial scale to produce natural bioactive compounds as basic ingredients for nutraceutical and pharmaceutical products with high economic value. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/JBBI/article/view/1793 10.55981/jbbi.2022.1793 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 1 (2022); 126-138 2548-611X 2442-2606 10.55981/jbbi.v9i1 eng https://ejournal.brin.go.id/JBBI/article/view/1793/1876 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/1794 2023-12-04T02:35:32Z JBBI:ART

CHANGES IN BLOOD GLUCOSE LEVELS IN EXPERIMENTAL ANIMALS WITH HYPERGLYCEMIA DUE TO LIME (Citrus aurantifolia Swingle) PEEL EXTRACT Suryadinata, Rivan Virlando Chronically elevated levels of glucose in the blood can lead to various complications. As a result, it can accelerate the damage to various organ tissues in the body. Several measures are needed to maintain the stability of normal blood glucose levels to prevent tissue damage, as well as providing additional intake such as lime peel extract which is believed to have high flavonoid content in lowering blood glucose levels. For this reason, this study aimed to analyse changes in blood glucose levels by giving lime extract to alloxan-induced experimental animals. This study uses an experimental post-test control group design. The results showed increased in blood glucose levels in the positive control group (p < 0.005). In addition, the lime (Citrus aurantifolia Swingle) peel extract treatment group showed changes in blood glucose levels (p < 0.005). It was concluded that increasing the intake of lime peel extract can reduce blood glucose levels. BRIN Publishing 2023-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/1794 10.55981/jbbi.2022.1794 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 9 No. 1 (2022); 139-146 2548-611X 2442-2606 10.55981/jbbi.v9i1 eng https://ejournal.brin.go.id/JBBI/article/view/1794/1875 https://creativecommons.org/licenses/by-nc-sa/4.0

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oai:ejournal.brin.go.id:article/2010 2023-12-29T15:35:21Z JBBI:ART

BIOTECHNOLOGY OF PRODIGIOSIN: RECENT DEVELOPMENTS AND TECHNOLOGICAL CHALLENGES Gunardi, Wani Devita Margaretha Timotius, Kris Herawan antibacteria anticancer antidiabetes Serratia marcescens solid state fermentation Background: Prodigiosin is produced by Serratia marcescens. It has several pharmacological benefits, such as anticancer, antimicrobial, and antidaibetic. However, prodigiosin production still faces problems because it cannot be produced effectively, efficiently, and cheaply. Objective: This study aimed to conduct a review that can explain the upstream and the downstream process in prodigiosin production. Methods: Articles were searched from PubMed and ScienceDirect with the keywords prodigiosin and Serratia marcescens from Juny until September 2023 including review and original article. Relevant data and information were then extracted. Results: Prodigiosin has spectrometrical characteristics, which are crucial for evaluating its production, extraction, and purification identification. Submerged or solid-state fermentation is applicable for prodigiosin production, but solid-state fermentation is better. The kind of growing substrates and the cultural condition influence it. The use of oil-based carbon sources is recommended for the high productivity of prodigiosin. In order to have a cheap, effective, and efficient production process, different experiments have been conducted. Standard extraction and purification methods can carry out the downstream process. Conclusion: Prodigiosin can be produced via submerged or solid-state fermentation. Using cheap and readily available substrate are the key to success for the upstream and downstream process. The standard extraction and purification methods are available.This findings can be used as a basis for further research regarding large-scale production of prodigiosin with the cheap, effective, and efficient methode. BRIN Publishing 2023-12-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2010 10.55981/jbbi.2023.2010 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 2 (2023); 251-269 2548-611X 2442-2606 10.55981/jbbi.v10i2 eng https://ejournal.brin.go.id/JBBI/article/view/2010/2126 Copyright (c) 2023 Wani Devita Gunardi, Margaretha, Kris Herawan Timotius https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/2013 2024-01-12T15:40:55Z JBBI:ART

THE EFFECT OF INCUBATION PERIOD ON ETHANOL CONTENT OF JASMINE AND GREEN TEA KOMBUCHA Ihsani, Nisa Hernahadini, Nelis Nurissalma, Zahra Salsabila Nurul Huda, Annisa Sartika, Tiwi Nadya Nur Afifah , Arifa Nadya Nur Afifah Rahma Nur Syamsiyah, Ninda ethanol fermentation incubation kombucha tea Kombucha is a fermented tea that provides many benefits for health. The alcohol (ethanol) content of kombucha has withdrawn a lot of attention because of human health risks and halal concerns. Alcohol and Tax and Trade Bureau (TTB), BC Center for Disease, and MUI fatwa have defined that kombucha is safe to be consumed as long as the alcohol content is less than 0.5%. This study was conducted to compare the alcohol (ethanol) concentration of jasmine and green tea kombucha during the fermentative stage (5 day at 25oC) and post-fermentative stage (7 and 21 day, both at 4oC). The result showed that ethanol concentrations for jasmine and green tea kombucha rose from the fifth d of incubation to the seventh d of the post-fermentative stage, followed by a decline at 21th d of the post-fermentative stage (p>0.05). The level of organic acids, Acetic Acid Bacteria (AAB), and pH confirmed that there were no significant differences at all (p>0.05). In conclusion, jasmine and green tea kombucha at fermentative stage (5 d at 25oC) and post-fermentative stage (7 and 21 day, both at 4oC) did not produce ethanol more than 0.5%, without any significant differences in organic acids and AAB levels, respectively. BRIN Publishing 2023-12-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2013 10.55981/jbbi.2023.2013 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 2 (2023); 361-367 2548-611X 2442-2606 10.55981/jbbi.v10i2 eng https://ejournal.brin.go.id/JBBI/article/view/2013/2151 Copyright (c) 2023 Nisa Ihsani, Nelis Hernahadini, Zahra Salsabila Nurissalma, Annisa Nurul Huda, Tiwi Sartika, Arifa Nadya Nur Afifah Nadya Nur Afifah , Ninda Rahma Nur Syamsiyah https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/2020 2023-12-29T15:35:21Z JBBI:ART

MICROBIAL L-ASPARAGINASES AND STRATEGIES TO IMPROVE THEM Achnafani, Dini Sari, Aniska Novita Anticancer E. coli E. chrysantemi L-asparaginase L-glutaminase L-asparaginase is a type of hydrolase enzyme that has been used in anticancer treatment, mainly Acute Lymphoblastic Leukemia (ALL). L-asparaginase reduces the blood supply of L-asparagine needed by cancer cells to survive. The commercially approved L-asparaginase by the FDA originated from E. coli and E. chrysantemi. However, reports of immunogenic effects in more than 50% of cases due to the use of these enzymes have become the driving force for the need to explore other sources of L-asparaginase. In this review, various alternative sources of L-asparaginase other than these two microbes will be explained. Microbes from the group of Gram-positive bacteria, actinomycetes, and fungi produce L-asparaginase with a higher affinity for L-asparagine than L-glutamine. Protein engineering is an alternative strategy to produce L-asparaginase that is not recognized by antibodies to reduce the immune reaction. Besides, the fermentation process also needs to be considered to determine the appropriate substrate and bioprocess system to obtain the enzyme. BRIN Publishing 2023-12-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2020 10.55981/jbbi.2023.2020 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 2 (2023); 236-250 2548-611X 2442-2606 10.55981/jbbi.v10i2 eng https://ejournal.brin.go.id/JBBI/article/view/2020/2125 Copyright (c) 2023 Dini Achnafani, Aniska Novita Sari https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/2379 2024-01-05T13:57:07Z JBBI:ART

EVALUATION OF ANTIBACTERIAL ACTIVITY OF PHYCOCYANIN PEPTIDES FROM SPIRULINA PLATENSIS THROUGH MULTIPLE MOLECULAR DOCKING STUDY Arindita, Ni Putu Yunika Munawaroh, Heli Siti Halimatul Aisyah, Siti Khoiriah, Selmi Fiqhi phycocyanin peptides Spirulina plantesis in silico analysis antibacterial protein-ligand interactions Peptides derived from the phycocyanin pigment of Spirulina plantesis were screened to identify potential natural antibacterial candidates. In silico protein hydrolysis was performed to obtain active peptides, and a multiple molecular docking (MLD) approach was used to to predict the synergistic mechanisms of various peptide combinations with their respective receptors. Nine phycocyanin peptides showed interaction values ranging from -5.1 to -6.0 kcal/mol, compared to the native ligand (BB-78485) with a value of -9.7 kcal/mol. The results demonstrated a synergistic effect, as the binding affinity of peptide combinations exceeded that of individual peptides and the native ligand. Hydrophobic bonds and van der Waals forces played a crucial role in binding to the active site of 2VES proteins. However, ADME studies revealed that two peptides, YCL and ASYF, had poor pharmacokinetic properties. Overall, this study highlights the potential of phycocyanin peptides as antibacterial agents through the application of MLD. BRIN Publishing 2023-12-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2379 10.55981/jbbi.2023.2379 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 2 (2023); 312-330 2548-611X 2442-2606 10.55981/jbbi.v10i2 eng https://ejournal.brin.go.id/JBBI/article/view/2379/2137 Copyright (c) 2023 Ni Putu Yunika Arindita, Heli Siti Halimatul Munawaroh, Siti Aisyah, Selmi Fiqhi Khoiriah https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/2478 2023-12-30T13:45:57Z JBBI:ART

POTENTIAL OF HAIR TONIC SARANG BANUA (Clerodendrum fragrans) LEAF EXTRACTS AS ANTI ALOPECIA IN VIVO Ananda, Zhafira Dwika Clarisa, Rahma Saragih, Ruth Yohana Hotang, Nadia Givani Br Hotang Lestari, Gusti Ayu Sinaga, Yesica Marcelina Romauli Simorangkir, Murniaty anti alopecia hair tonic in vivo Rattus norvegicus sarang banua (Clerodendrum fragrans) Alopecia merupakan suatu keadaan hilang atau tidak tumbuhnya rambut kepala yang dapat terjadi akibat dari penuaan, kekurangan gizi, ketidakseimbangan hormon. Hair tonic merupakan sediaan cair yang digunakan sebagai penumbuh dan penyubur rambut. Sediaan hair tonic sebagai penumbuh rambut membutuhkan antioksidan yang tinggi. Sarang banua (Clerodendrum fragrans) memiliki antioksidan yang tinggi. Riset ini bertujuan untuk mengetahui potensi hair tonic ekstrak daun sarang banua sebagai anti alopecia secara in vivo. Metode riset dilakukan secara in vivo pada tikus putih wistar (Rattus norvegicus). Penelitian ini merupakan penelitian eksperimental Rancangan Acak Lengkap (RAL) dengan 5 kelompok, K– (tanpa ekstrak), K+ (minoxidil 2%), F1 (5% ekstrak), F2 (7,5% ekstrak), dan F3 (10% ekstrak) dilakukan 3 perlakuan. Perlakuan dilakukan setiap hari dioleskan ke tikus yang mengalami stress dan kebotakan alami. Hasil uji ANOVA menunjukkan bahwa F1 (ekstrak 5%) tidak ada perbedaan signifikan dengan K+ (minoxidil 2%). Alopecia is a condition where head hair is missing or not growing which can occur as a result of aging, malnutrition, hormonal imbalance. Hair tonic is a liquid preparation that is used to grow and fertilize hair. Hair tonic preparations as hair growth require high levels of antioxidants. Sarang Banua (Clerodendrum fragrans) contains has high antioxidant. This research aims to determine the potential of hair tonic sarang banua leaf extract as an anti-alopecia in vivo. The research method is in vivo with white wistar rats (Rattus norvegicus). This research was an experimental LSD with 5 groups, K– (no extract), K+ (minoxidil 2%), F1 (5% extract), F2 (7.5% extract), and F3 (10% extract) carried out 3 treatments. The treatment is carried out every day and applied to mice that experience stress and natural baldness. The ANOVA test results showed that F1 (5% extract) had no significant difference from the K+ (2% minoxidil). BRIN Publishing 2023-12-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2478 10.55981/jbbi.2023.2478 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 2 (2023); 304-311 2548-611X 2442-2606 10.55981/jbbi.v10i2 eng https://ejournal.brin.go.id/JBBI/article/view/2478/2130 Copyright (c) 2023 Zhafira Dwika Ananda, Rahma Clarisa, Ruth Yohana Saragih, Nadia Givani Br Hotang Hotang, Gusti Ayu Lestari, Yesica Marcelina Romauli Sinaga, Murniaty Simorangkir https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/2546 2024-11-01T16:20:34Z JBBI:ART

THE RELATIONSHIP OF TNFα -308 G/A POLYMORPHISM WITH THE INCIDENCE OF CERVICAL CANCER IN ASIAN WOMEN: A META ANALYSIS OBSERVATIONAL STUDY Saraswati, Henny Nurmalasari, Mieke Asian women cervical cancer allele TNFa polymorphism genotype Cervical cancer is a malignancy with high mortality rates in women, and its incidence continues to rise. The main etiological factor for cervical cancer is infection with Human Papillomavirus (HPV), which disrupts the regulation of apoptosis in cells. Several studies have shown a correlation between TNFα polymorphisms, including the -308 position (TNFα -308 G/A), and the incidence of cervical cancer.This gene have a role in proliferation of cancer cells. This study investigates the impact of TNFα-308 polymorphism on the risk of cervical cancer in Asian female populations. A meta-analysis of five sources was conducted to determine potential associations. Findings reveal that neither allele A (OR 95%CI = 1.20 [0.70-2.03], p = 0.51) nor genotype AA (OR 95%CI = 0.85 [0.37-1.91], p = 0.69) were significantly linked with an elevated risk of cervical cancer in Asian women. The same result was seen for the G allele (OR 95%CI = 0.84 [0.49-1.42], p = 0.51) and GG genotype (OR 95%CI = 0.80 [0.44-1.48], p = 0.48). The study results indicate that the TNFα-308 polymorphism is not associated with cervical cancer in Asian women. Further research is needed to investigate the role of other gene polymorphisms in cervical cancer susceptibility in Asian women. BRIN Publishing 2024-10-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2546 10.55981/jbbi.2024.2546 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 188-198 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/2546/6564 Copyright (c) 2024 Henny Saraswati, Mieke Nurmalasari https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/2549 2023-12-19T21:40:26Z JBBI:ART

EVALUATING THE LONG-TERM STORAGE TIME VIABILITY AND SIZE DYNAMICS OF BACILLUS SP. BIOENCAPSULATION IN SODIUM ALGINATE MATRIX Saputra, Mochammad Mirza Wuryandari, Yenny Rahmadhini, Noni Lestari, Safira Rizka Bacillus sp. Bioencapsulation Sodium Alginate Viability Beads Size The use of biological agents such as Bacillus sp. bacteria has begun to be widely used by farmers as a new form of control. Bacillus sp. needs to require special methods in its application because it has certain living conditions, and Bacillus sp. is vulnerable to environmental pressures. Bioencapsulated formulation in the form of granules is considered effective as a form of bacterial application in soil because it is able to protect Bacillus sp. and maintain survival. This study aims to determine the best concentration of sodium alginate in maintaining the viability of Bacillus sp. at the in vitro stage and to see changes in the size of the beads. The beads were made using extrusion method by combining Bacillus sp. suspension and sodium alginate suspension at 1%, 1.5%, and 2% concentration. The concentration of sodium alginate was not a major factor in changes in bead size and viability test results experienced significant changes in each observation. 1.5% and 2% concentrations can reach the highest viability value of 3x106 CFU/mL and decreased during 1 month of observation. Changes in bead size and viability were caused by the alginase enzyme produced by Bacillus sp. BRIN Publishing 2023-12-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2549 10.55981/jbbi.2023.2549 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 2 (2023); 211-219 2548-611X 2442-2606 10.55981/jbbi.v10i2 eng https://ejournal.brin.go.id/JBBI/article/view/2549/2022 Copyright (c) 2023 Mochammad Mirza Saputra, Yenny Wuryandari, Noni Rahmadhini, Safira Rizka Lestari https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/2557 2024-01-05T13:02:15Z JBBI:ART

DEVELOPMENT OF PLASMID-BASED FOR EXTERNAL CONTROL MATERIALS OF CYP2D6*10 (rs1065852) GENE PCR-BASED DETECTION Malau, Jekmal Zahra, Aliya Azkia Kasasiah, Ahsanal Rahmasari, Ratika Raekiansyah, Muhareva Rohmah, Siti Meilani, Nanda Diva Septi, Annisa Frastica Zahro, Aurora Fatimatuz Annajla, Fathina Hermosaningtyas, Anastasia Aliesa Hilmi, Indah Laily Plasmid recombinant external control material Single Nucleotide Polymorphisms (SNPs) CYP2D6*10 rs1065852 Clinical diagnostic Reliable clinical diagnosis of Single Nucleotide Polymorphisms (SNPs) is necessary for personalizing tamoxifen medication according to CYP2D6*10 genetic variations. Our research aimed to create a recombinant plasmid for external control material with a molecular size of 3812 bp. The recombinant plasmid was achieved by cloning an 838 bp gene insert of CYP2D6*10 rs1065852 into a 2974 bp pJET1.2 plasmid into Escherichia coli DH10B and selection on ampicillin agar medium. Isolated E. coli recombinants provided the plasmid molecules for analysis. Bi-directional sequencing and Real-Time PCR confirmed the presence of wild-type and mutant rs1065852 DNA fragments in the plasmid, namely homozygote CC and TT. The conclusion is that we have successfully introduced a novel recombinant plasmid developed by cloning the SNP rs1065852, which carries the 100C>T mutation, using pJET 1.2/blunt system, which could significantly enhance the accuracy of clinical SNP diagnostics for personalized medicine in breast cancer treatment. BRIN Publishing 2023-12-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2557 10.55981/jbbi.2023.2557 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 2 (2023); 220-235 2548-611X 2442-2606 10.55981/jbbi.v10i2 eng https://ejournal.brin.go.id/JBBI/article/view/2557/2124 Copyright (c) 2023 Jekmal Malau, Aliya Azkia Zahra, Ahsanal Kasasiah, Ratika Rahmasari, Muhareva Raekiansyah, Siti Rohmah, Nanda Diva Meilani, Annisa Frastica Septi, Aurora Fatimatuz Zahro, Fathina Annajla, Anastasia Aliesa Hermosaningtyas, Indah Laily Hilmi https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/2610 2023-12-19T21:42:05Z JBBI:ART

ANALYSIS OF INTERLEUKIN-10 LEVELS IN MESENCHYMAL STEM CELL SECRETOME CREAM WITH ELISA METHOD Amelia, Vika Marlina Sudji, Ikhwan Resmala Cream Inflammation Interleukin-10 Mesenchymal Stem Cell secretome Mesenchymal Stem Cells (MSC) are cells that are able to differentiate into other cells and have immunomodulatory properties that are used to treat inflammatory diseases. MSCs have the ability to repair damaged tissue by regenerating damaged tissue. MSCs produce a secretome, where the MSC secretome has various cytokines, chemokines, growth factors, anti-inflammatory factors and proteins that are produced in molecular form. One of the molecules secreted by the secretome is Interleukin-10.IL-10 is an anti-inflammatory cytokine which functions to inhibit the production of several other types of cytokines such as TNF, IL-1, chemokine and IL-12). Apart from that, IL-10 is able to inhibit the function of macrophages in assisting T cell activity. One of the products from Secretome MSC is a cream preparation. To find out whether the MSC cream contains IL-10 protein, Interleukin-10 levels were analyzed in the MSC secretome cream with 3 different MSC cream concentration formulations (2%, 3% and 5%). Analysis of IL-10 levels was carried out using ELISA to detect IL-10 antigen. The results of the examination of 9 samples showed that all samples were positive for IL-10 with a concentration of 2% dilution 1:2 showing the best results with an Optical Density (OD) of 0.069 and followed by a concentration of 5% dilution 1:2 showing an OD value of 0.065. BRIN Publishing 2023-12-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2610 10.55981/jbbi.2023.2610 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 2 (2023); 203-210 2548-611X 2442-2606 10.55981/jbbi.v10i2 eng https://ejournal.brin.go.id/JBBI/article/view/2610/2021 Copyright (c) 2023 Vika Amelia, Marlina, Ikhwan Resmala Sudji https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/2803 2023-12-30T13:41:19Z JBBI:ART

ANALYSIS OF BIOMETHANE PRODUCTION POTENTIAL AND MITIGATION OF BIOMETHANE EMISSIONS FROM PIG FARMS IN NORTH SULAWESI PROVINCE Manullang, Efrando Sutaryo Kusdiyantini, Endang The potential for biomethane production from livestock manure as renewable energy has good prospects, considering that the number of pigs in North Sulawesi Province increases yearly. Therefore, it is necessary to analyze the potential for biomethane production, replacing the use of LPG, and mitigate biomethane emissions from pig farms. The results showed that from 2015-2021, the most significant biomethane production was in 2021, amounting to 7,887 tons of CH4/year in the Minahasa Regency area, 2,496 tons of CH4/year (31.65%) in Tomohon City, 805 tons of CH4/year (10.20%) in South Minahasa Regency, 779 tons of CH4/year (9.88%), and in other areas 3,807 (48.27%). Biomethane can replace 8.59 million kg of Liquefied Petroleum Gas (LPG). The results of mitigating biomethane emissions in 2021 reached 34.22 Gg CO2eq, and using biomethane to replace LPG reached 11.59 Gg CO2eq. Total greenhouse gas emissions can be reduced to 45.81 Gg CO2eq. Biomethane from pig manure has promising potential as a new renewable energy and requires further investigation. BRIN Publishing 2023-12-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2803 10.55981/jbbi.2023.2803 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 2 (2023); 279-292 2548-611X 2442-2606 10.55981/jbbi.v10i2 eng https://ejournal.brin.go.id/JBBI/article/view/2803/2128 Copyright (c) 2023 Efrando Manullang, Sutaryo, Endang https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/2840 2023-12-09T10:40:59Z JBBI:ART

MOLECULAR IDENTIFICATION OF PHOSPHATE SOLUBILIZING BACTERIA FROM PGPR PRODUCT DERIVED FROM TEMANGGUNG REGENCY Akbar, Muhammad Falih Purwantisari, Susiana Pujiyanto, Sri Ferniah, Rejeki Siti Phosphate Solubilizing Bacteria Bacillus subtilis strain SBMP4 PCR 16S rRNA Gene PGPR T Phosphate solubilizing bacteria are soil bacteria that can dissolve phosphates from insoluble phosphate bonds so that they can be absorbed by plants. Phosphates in the soil are naturally present in organic and inorganic forms. Both forms are insoluble or slightly soluble forms of phosphate, so their availability for soil biota is very limited. This study aimed to identify the phosphate solubilizing bacteria from locally PGPR product from Temanggung, Indonesia, namely BPF 1. The method in this study was carried out by molecular identification using the 16S rRNA encoding gene which includes bacterial DNA isolation, DNA amplification using PCR, bacterial DNA sequencing and the creation of phylogenetic trees. The results of the study were obtained isolate bacteria solubilizing phosphate BPF 1 from PGPR products of local farmers of Temanggung Regency identified as the genus Bacillus, having the closest kinship with B. subtilis strain SBMP4 (NR_118383.1) with a similarity of 98.42%. BRIN Publishing 2023-12-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2840 10.55981/jbbi.2023.2840 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 2 (2023); 150-157 2548-611X 2442-2606 10.55981/jbbi.v10i2 eng https://ejournal.brin.go.id/JBBI/article/view/2840/1943 Copyright (c) 2023 Muhammad Falih Akbar, Susiana Purwantisari, Sri Pujiyanto, Rejeki Siti Ferniah https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/2841 2026-06-24T14:17:40Z JBBI:ART

IN-SILICO ANALYSIS OF SYMBIONT BACTERIA DIVERSITY IN THE MIDGUT OF Aedes aegypti USING 16S rDNA MOLECULAR MARKERS DATABASE Wathon, Syubbanul Finasrullah, Aufar Oktarianti, Rike Senjarini, Kartika bacteria bioinformatics database dengue symbiont Dengue Hemorrhagic Fever (DHF) is caused by the dengue virus, which is transmitted through Aedes aegypti mosquitoes when they feed on human blood.  To effectively control the DHF vector, it is crucial to accurately characterize the symbiont bacteria associated with Ae. aegypti through an in-silico approach to identify potential targets. This study utilized in- silico analysis based on the 16S rDNA molecular marker to explore the diversity of symbiont bacteria obtained from bioinformatics databases. The analysis and visualization of bacterial diversity were conducted using the Pathosystem Resource Integration Center (PATRIC). The analysis results revealed that bacterial diversity in the midgut of Ae. aegypti, categorized as culturable and non-culturable bacteria, exhibited similar abundance patterns at the family level, albeit with varying detection rates. The most dominant taxa included the phylum Proteobacteria, class Gammaproteobacteria, order Enterobacterales, and family Enterobacteriaceae. Within the culturable bacteria category, the dominant taxa were the genus Salmonella and species Salmonella enterica, whereas the non-culturable bacteria category indicated the prevalence of the genus Escherichia and species Escherichia coli. BRIN Publishing 2023-12-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2841 10.55981/jbbi.2023.2841 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 2 (2023); 158-173 2548-611X 2442-2606 10.55981/jbbi.v10i2 eng https://ejournal.brin.go.id/JBBI/article/view/2841/1944 Copyright (c) 2023 Syubbanul Wathon, Aufar Finasrullah, Rike Oktarianti, Kartika Senjarini https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/2842 2023-12-10T02:37:43Z JBBI:ART

EVALUATION AND INHIBITORY MECHANISM ANALYSIS OF NATURAL COMPOUNDS AGAINTS DIHYDROOROTATE DEHYDROGENASE AS ANTI-CANCER AGENTS Wibowo, Aji Waluyo, Danang Tejo, Bimo Ario Komariyah, Tinta Besari, Ariza Yandwiputra Prabandari, Erwahyuni Endang Anticancer Drug discovery Dihydroorotate dehydrogenase Molecular docking Molecular dynamic Cancer remains one of the deadliest diseases worldwide, and currently cancer treatment is facing several problems related to adverse effects and drug resistance. To address these problems, new prospective anticancer medications are required. Natural compounds, which have been extensively used in the drug research, including for the treatment of cancer, are emerging as viable candidates. This study aimed to evaluate 33 in-house natural compounds against dihydroorotate dehydrogenase (DHODH) enzyme, a viable target to develop anticancer agent, and to analyze the hit inhibitory mechanism against protein target. In the activity assay, atovaquone was the sole substance to have activity against DHODH, with an inhibition rate of 47.44% at 10 µM. However, discrepancies were shown in the molecular docking result, where atovaquone were identified as hits. Molecular dynamic analysis revealed that atovaquone initially bound to the active site before being forced to the outside due to cleavage of hydrogen bond between the ligand and responsible residue. This study clearly demonstrated the importance of molecular dynamic analysis to study inhibitory mechanism of compound against target protein that may be useful for further development. BRIN Publishing 2023-12-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2842 10.55981/jbbi.2023.2842 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 2 (2023); 174-202 2548-611X 2442-2606 10.55981/jbbi.v10i2 eng https://ejournal.brin.go.id/JBBI/article/view/2842/1945 Copyright (c) 2023 Aji Wibowo, Danang Waluyo, Bimo Ario Tejo, Tinta Komariyah, Ariza Yandwiputra Besari, Erwahyuni Endang Prabandari https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/2851 2023-12-07T14:11:34Z JBBI:ART

INHIBITORY ACTIVITY OF INDONESIAN MICROBIAL EXTRACTS AGAINST PROLIFERATION OF DLD-1 COLORECTAL CANCER CELL LINE Besari, Ariza Yandwiputra Waluyo, Danang Komariyah, Tinta Prabandari, Erwahyuni Endang Colorectal cancer (CRC) is the second deadliest cancer in the world. Several anti-cancer agents are currently used for the clinical treatment of CRC. However, toxicity and drug resistance pose significant challenges in CRC chemotherapy. On the other hand, microbe-derived natural products have been explored as a source for the development of anti-cancer therapeutic agents. This study aimed to examine the potential of the microbial library in BioMCC (Biotech Center-BPPT Microbial Culture Collection) as a source for anti-cancer drug discovery. Among the 720 fungal extracts tested, 60 extracts (8.3%) showed inhibitory activity against the proliferation of the colorectal carcinoma DLD-1 cell line, while not affecting Vero cells (African green monkey kidney normal cell line). One of these active extracts was derived from the fungus Sporothrix sp. BioMCC-f.T.7716. Although the inhibitory mechanism of this extract against the proliferation of the DLD-1 cell line could not be determined, this study clearly demonstrated the potential use of Indonesian microbial extracts as a source for the discovery of anti-cancer agents. BRIN Publishing 2023-12-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2851 10.55981/jbbi.2023.2851 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 1 (2023); 1-10 2548-611X 2442-2606 10.55981/jbbi.v10i1 eng https://ejournal.brin.go.id/JBBI/article/view/2851/1918 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/2946 2023-12-29T15:35:21Z JBBI:ART

ANALYSIS OF KIDNEY HISTOPATHOLOGY IN SEPSIS MODEL OF MICE (MUS MUSCULUS) WITH PREVENTIVE TREATMENT OF LEAF EXTRACT FROM 'DAUN KENTUT' PLANT (PAEDERIA FOETIDA L.) AGAINST ESCHERICHIA COLI INFECTION Savitri, Lisa Kasimo, Elfred Rinaldo Krissanjaya, Rochmad Sukmawati, Datin An Nisa Juwita, Syntia Tanu Kholis, Akbar Nur Mosse1, Yosep kidney histopathology sepsis daun kentut Escherichia coli Sepsis is a critical health concern characterized by organ dysfunction due to uncontrolled host reactions to infections. The prevalence of microorganisms causing sepsis varies, with Gram-positive bacteria and fungal sepsis gaining significance. Sepsis often leads to acute kidney injury (AKI), and its association with sepsis is known as sepsis-associated AKI (SA-AKI). The "Daun Kentut" plant, known for its diverse bioactive compounds, has shown potential in combating inflammation and oxidative stress. In this study, histopathological changes in mouse kidneys induced by Escherichia coli were investigated. Hemorrhage was the primary change observed. Dehydration caused by E. coli infection potentially led to kidney injury through hormonal responses. The plant extract's anti-inflammatory potential, attributed to compounds like saponins and flavonoids, was also explored. The study emphasizes the need for understanding sepsis-related kidney damage and suggests possible preventive strategies using natural compounds. BRIN Publishing 2023-12-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2946 10.55981/jbbi.2023.2946 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 2 (2023); 270-278 2548-611X 2442-2606 10.55981/jbbi.v10i2 eng https://ejournal.brin.go.id/JBBI/article/view/2946/2127 Copyright (c) 2023 Lisa Savitri, Elfred Rinaldo Kasimo, Rochmad Krissanjaya, Datin An Nisa Sukmawati, Syntia Tanu Juwita, Akbar Nur Kholis, Yosep Mosse1 https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/2947 2023-12-30T13:43:42Z JBBI:ART

BACTERIAL CONTAMINATION TEST IN POWDER-FORMULATED Helicoverpa armige-ra NUCLEAR POLYHEDROSIS VIRUS (HaNPV1) SUBCULTURE Miranti, Mia Kasmara, Hikmat Fitriani, Nurullia Melanie, Melanie A'yun, Inas Qurrata Syaputri, Yolani Doni, Febri Madihah, Madihah Rahayuningsih, Sri Rejeki Azizah, Nabilah Sekar Hermawan, Wawan Bacterial contaminants biocontrol agent carrier medium gypsum talcum The Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV1) is a subculture derived from the original HaNPV, and it has been cultivated in Spodoptera litura larvae as an alternative host. HaNPV1 was subsequently formulated using gypsum and talcum as carrier media. Following this formulation, a bacterial contamination test was conducted to assess the quality of the viral formulation.  The experiment was arranged in the randomized factorial block design (RFBD) with 2 replications. The viral formulations was stored for 16 weeks and the samples were taken every two weeks for contamination analysis. The data was then analyzed with the analysis of variance (ANOVA) and a post-hoc using Duncan’s Multiple Range test. The variable observed was the number of the bacterial colonies cultivated on the specific media i.e., Nutrient Agar (NA), Salmonella Shigella Agar (SSA) and Eosin Methilen Blue Agar (EMB). The results showed that the bacterial contaminants was detected from 0 to 12 weeks of storage time. However, the highest contamination was found in viral formulation after 8 weeks of storage time and the highest bacterial contaminations were recorded from all viral formulation tested in NA. The results indicated that the bacterial contamination were found around 1.45 × 109 cfu/gram and 1.97 × 109 cfu/gram in gypsum and talcum formulations, respectively. On SSA and EMB media, the bacteria contaminants from all formulation found in 8 weeks of storage time, but Salmonella, Shigella, or Escherichia coli (aspathogenic bacteria) were not found. After 12 weeks storage time, there was no indication of  contamination found in all media. Furthermore, Bacillus species was found as a most dominant contaminant in all samples. In conclusion, although the viral formulations using gypsum and talc were not contaminated by pathogenic bacteria such Salmonella, Shigella, or E. coli. Nevetherless, the viral formulation was still easily contaminated by other non-pathogenic bacterial species. Thus, a more standardized and stricted strategy needs to be developed for a better viral formulation product. BRIN Publishing 2023-12-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2947 10.55981/jbbi.2023.2947 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 2 (2023); 293-303 2548-611X 2442-2606 10.55981/jbbi.v10i2 eng https://ejournal.brin.go.id/JBBI/article/view/2947/2129 Copyright (c) 2023 Mia Miranti, Hikmat Kasmara, Nurullia Fitriani, Melanie, Inas Qurrata A'Yun, Yolani Syaputri; Febri Doni; Madihah, Sri Rejeki Rahayuningsih; Nabilah Sekar Azizah; Wawan Hermawan https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/2949 2024-11-01T16:47:57Z JBBI:ART

IDENTIFICATION OF INDONESIAN ETHNOMEDICINAL PLANTS AS POTENTIAL DRUG CANDIDATES FOR ACUTE RESPIRATORY INFECTION USING COMPUTER-AIDED DRUG DESIGN AND SIMRS MODEL Jackson, James Tanaya, Davina Nadine Setiawan, Stefanus Acute Respiratory Infection cefuroxime ethnomedicinal plants in silico time series SIMRS model In 2023, Indonesia’s air quality deteriorated, with its Air Quality Index (AQI) tripling clean air standards, causing health sector losses, including a surge in Acute Respiratory Infection (ARI) cases. One of the ARI treatments is the consumption of cefuroxime, yet it can cause side effects. Indonesia’s floral biodiversity in ethnomedicinal plants can be utilized as a more natural drug candidate for ARI drugs. To determine this, an in silico approach is performed through molecular docking, and Pre-ADMET prediction. Based on the compound selection’s results, lanosterol is the most promising compound, with a binding energy value of -8.11 kcal/mol and an efficiency of 78.81%, while cefuroxime as a reference ligand has a binding energy value of -5.92 kcal/mol with an efficiency of 67.87%. After undergoing compound selection, a time series analysis through the Susceptible Infected Medicine Recovered Susceptible (SIMRS) model is conducted. In this analysis, it is found that cefuroxime and lanosterol require the same amount of time, which is 33 days to restore Indonesia to its pre-ARI outbreak condition, indicating that lanosterol can be used as an alternative drug candidate. BRIN Publishing 2024-10-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2949 10.55981/jbbi.2024.2949 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 223-235 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/2949/6656 Copyright (c) 2024 James Jackson, Davina Nadine Tanaya, Stefanus Setiawan https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/2950 2025-01-16T04:04:55Z JBBI:ART

STUDY OF ANTI-BACTERIAL AND ANTI-INFLAMMATORY PROPERTIES OF INDONESIA’S FLORA BIODIVERSITY AS DRUG CANDIDATES FOR DIABETIC ULCER THROUGH IN SILICO AND IN VITRO APPROACH Tiffanny, Cecilia Regina, Aurelia Chen, Davin Handreas Rosyati, Margaretha Maria anti-bacteria anti-inflammation diabetic ulcer in silico in vitro This study is driven by the substantial increase in the number of diabetes mellitus patients, which is projected to triple by 2030 and the immense potential of Indonesia's extensive biodiversity. Diabetic ulcers represent one of the most common complications stemming from uncontrolled diabetes mellitus. Therefore, this research aims to determine the therapeutic potential of Indonesian biodiversity for diabetic ulcers, focusing on the anti-bacterial and anti-inflammatory properties of secondary metabolite derivatives, employing both in silico and in vitro approaches. Initially, the research employed an in silico approach, conducting molecular docking and molecular dynamics simulations on reference ligands obtained from the 6KVS receptor for Staphylococcus aureus bacteria and the 5KIR for the COX-2 protein. Molecular docking was performed for 18 secondary metabolite derivative test compounds using Autodock Vina, followed by molecular dynamics simulations. The results of the molecular dynamics simulations showed that flavanonols exhibited the highest stability among the test compounds, with an average root mean squared deviation of 0.139493 Å for anti-bacterial tests and 0.182499 Å for anti-inflammatory tests after 50 ns simulation. Based on the results of molecular dynamics simulations, in vitro anti-bacterial and anti-inflammatory testing was conducted using flavanonol compounds found in Ananas comosus plants. In the anti-bacterial test, the pineapple extract, at a 5% concentration, exhibited the largest inhibitory zone diameter, 3.53 mm. In the anti-inflammatory tests, the pineapple extract at an optimal concentration of 600 ppm showed the highest absorbance value for protein denaturation, measuring 0.11133 au. BRIN Publishing 2024-11-16 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2950 10.55981/jbbi.2024.2950 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 2 (2024); 244-261 2548-611X 2442-2606 10.55981/jbbi.v11i2 eng https://ejournal.brin.go.id/JBBI/article/view/2950/6831 Copyright (c) 2024 Cecilia Tiffanny, Aurelia Regina, Davin Handreas Chen, Margaretha Maria Rosyati https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/2976 2024-01-12T15:40:55Z JBBI:ART

DETERMINATION OF OPTIMAL ANNEALING TEMPERATURE Vibrio alginolyticus PRIMERS USING POLYMERASE CHAIN REACTION METHOD Putri, Gladys Indira Nurjayadi, Muktiningsih Declan, Jefferson Lynford Krisdawati, Ismaya Juliansyah, Dandy Akbar Fahriza, Tiara Azzahra, Maharanianska Maulana, Irvan Kartika, Irma Ratna Kurniadewi, Fera Sukmawati, Dalia Saamia4, Vira Saputro, Dwi Anna Oktaviani Wiranatha, I Made Abomoelak, Bassam El-Enshasy, Hesham Ali Annealing temperature Foodborne diseases Polymerase Chain Reaction Va_Chr1_FR primers Vibrio alginolyticus Food poisoning is a global issue of grave concern. If food is not properly cooked, it can be a medium for the spread of pathogenic bacteria. Vibrio alginolyticus is one of the pathogenic bacteria that can cause food poisoning. real-time Polymerase Chain Reaction (rt-PCR) can detect pathogenic bacteria in food, so it is necessary to determine the optimal annealing temperature. This research aims to obtain the optimal annealing temperature of the Va_Chr1_FR primer using Gradient PCR. The DNA concentration used was 174.5 with an A260/A280 purity of 1.94. The temperature range tested, 53°C-62°C, corresponds to the melting temperature of the Va_Chr1_FR primers. The primers designed were F5'-TTCTTCTGTTGTAGGTTCCG-F3' and R5'-CCAGCCCTCACATCTAATAC-R3'. Based on these results, a temperature of 60°C is deemed as the most optimal annealing temperature because it produces one of the brightest bands on electrophoresis with an amplicon length of 146 bp. The findings of this study will be beneficial to the development of Va_Chr1_FR Vibrio alginolyticus primers testing on food samples using the real-time PCR method.  BRIN Publishing 2023-12-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2976 10.55981/jbbi.2023.2976 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 2 (2023); 346-354 2548-611X 2442-2606 10.55981/jbbi.v10i2 eng https://ejournal.brin.go.id/JBBI/article/view/2976/2149 Copyright (c) 2023 Gladys Indira Putri, Muktiningsih Nurjayadi, Jefferson Lynford Declan, Ismaya Krisdawati, Dandy Akbar Juliansyah, Tiara Fahriza, Maharanianska Azzahra, Irvan Maulana, Irma Ratna Kartika, Fera Kurniadewi, Dalia Sukmawati, Vira Saamia4, Dwi Anna Oktaviani Saputro, I Made Wiranatha, Bassam Abomoelak, Hesham Ali El-Enshasy https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/2977 2024-01-12T15:40:55Z JBBI:ART

THE PATHOPHYSIOLOGY OF CUTANEOUS MANIFESTATIONS RELATED TO COVID-19 Mawardi, Prasetyadi Covid-19 pathophysiology skin abnormalities skin manifestations Covid-19 is a respiratory infection caused by the newly emerging coronavirus. Covid-19 can also cause skin abnormalities, although the pathophysiology of manifestations of skin disorders associated with Covid-19 has not been understood with certainty. This study aims to discuss the pathophysiology of cutaneous manifestation related to Covid-19. The study was conducted based on literature and data mining related to Covid-19 using PubMed and Google Scholar. Eligible papers provided pathophysiology of cutaneous manifestations related to Covid-19. Papers not available in English were excluded. As a result of the presence of edema, vasodilation, and cellular infiltration, skin lesions such as rash, urticarial, or extensive purpura, or even thrombosis and haemorrhage, arise if damage to the vessels became severe. The pathophysiology of Covid-19-associated skin manifestations is estimated through some different mechanisms, such as lesions found in various Covid-19 patients, namely, the presence of varicella such as eruption, non-characteristic rash, and urticarial. Further studies are required to establish a Covid-19 diagnosis based on skin manifestations. BRIN Publishing 2023-12-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/2977 10.55981/jbbi.2023.2977 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 2 (2023); 342-345 2548-611X 2442-2606 10.55981/jbbi.v10i2 eng https://ejournal.brin.go.id/JBBI/article/view/2977/2148 Copyright (c) 2023 Prasetyadi Mawardi https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/3005 2025-01-16T04:06:40Z JBBI:ART

MOLECULAR CHARACTERIZATION, PHYTOCHEMICALS SCREENING, AND MOLECULAR DOCKING OF CARDAMOM (Wurfbainia compacta), AND SAMBILOTO (Andrographis paniculata) AGAINST COVID-19 Kurniawati, Mufida Budi Budiharjo, Anto Kusumaningrum, Hermin Pancasakti Ferniah, Rejeki Siti Phytopharmaceutical Phytochemical Cardamom Sambiloto Molecular Docking Cardamom and Sambiloto are phytopharmaceutical plants that produce phytochemical compounds that have the potential to be used to increase immunity against COVID-19. because they contain carotenoids, phenols, anthocyanins, saponins, alkaloids and steroids. This research aims to obtain the molecular characteristics of Cardamom and Sambiloto plants from the Gunungpati area, Semarang by ITS primer, testing phenolic phytochemicals, tannins, flavonoids, saponins and alkaloids followed by molecular docking tests with the 6WX4 protein. SARS-CoV-2. Molecular characterization results show that Cardamom and Sambiloto are similar to Wurfbainia compacta MF802556.1 (100%) and Andrographis paniculate LC646073.1 (84.47%). The results of the phytochemical test screening showed that both plants contain flavonoids. Molecular docking tests were carried out with the compounds Quercetin, Avicularin, Naringenin, 5-hydroxy-7,8,2',5' tetramethoxyflavone, and Retinoic Acid. Retinoic Acid as a test ligand has the greatest potential in inhibiting the 6WX4 protein in the SARS-CoV-2 virus with a binding affinity value of -7.28 and RMSD 0.00. BRIN Publishing 2024-11-16 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/3005 10.55981/jbbi.2024.3005 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 2 (2024); 278-289 2548-611X 2442-2606 10.55981/jbbi.v11i2 eng https://ejournal.brin.go.id/JBBI/article/view/3005/6833 Copyright (c) 2024 Mufida Budi Kurniawati, Anto Budiharjo, Hermin Pancasakti Kusumaningrum, Rejeki Siti Ferniah https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/3021 2024-11-01T16:20:34Z JBBI:ART

ANALYSIS AND POTENTIAL THE ECO-ENZYME OF SEVERAL TYPES OF FRUIT PEELS IN LEACHATE REMEDIATION Rasyidah Nasution, Rizki Amelia Organic Matters Eco enzyme Leachate Chemical Compounds This research aims to determine the potential of ecoenzymes from several types of fruit peel in the process of reducing TDS levels of COD, BOD, ammonium and phosphate as well as the number of bacterial colonies in leachate water. So the research involved activities to make ecoenzymes from 4 types of fruit peel, namely banana, dragon fruit, pineapple and orange. Next, the ecoenzyme results were applied to leachate water to test its remediation potential in a ratio of 1:2 (10% ecoenzyme). This research uses a descriptive method, which uses parameters namely TDS, COD, BOD, phosphate, ammonium, and counting bacterial colonies. The results show that ecoenzymes have the potential to remediate leachate waste during five days of incubation, where it can be seen that ecoenzymes can reduce ammonium and phosphate levels but not BOD and COD. From the results, the average reduction in ammonia levels was higher for ecoenzymes from banana peel and pineapple fruit with an average value (mg/l) of P1=14.6; P2=0.55; P3=1.44; P4=0.675 and P5=2.44 (P1: Leachate; P2: Leachate + banana peel ecoenzyme; P3: Leachate + Dragon Fruit peel ecoenzyme; P4: Leachate + Pineapple Peel; P5: Leachate + Fruit peel Orange). Meanwhile, the highest reduction in phosphate was found in remediation using ecoenzymes from orange peel where the average value was P5=3.05 < P1=8.2. The results of ecoenzyme remediation from four types of fruit peel only have the potential to reduce ammonia levels, and likewise the reduction in phosphate occurs after adding orange peel ecoenzymes. BRIN Publishing 2024-07-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/3021 10.55981/jbbi.2024.3021 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 33-42 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/3021/4245 Copyright (c) 2024 Rasyidah, Rizki Amelia Nasution https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/3080 2024-11-01T16:20:34Z JBBI:ART

SYNTHESIS AND CHARACTERIZATION OF CHITOSAN MEMBRANES OF CASSAVA PEEL (Manihot esculenta) AND LERI STARCH COMBINATION AS A BIODEGRADABLE PLASTIC: English Dewi, Pande Putu Indira Prima Widhiantara, I Gede Sandhika, I Made Gde Sudyadnyana Wiradana, Putu Angga Susilo, Raden Joko Kuncoroningrat Biodegradable Characterization Starch Chitosan Cassava peel Problems related to landfills of plastic packaging waste are a source of environmental pollution. This research provides innovation in utilizing biodegradable plastic from cassava and leri peel starch with the addition of chitosan. This study aimed to analyze the results of the synthesis and characterization of chitosan membranes with a combination of cassava and leri peel starch and determine the biodegradation time of biodegradable plastic in EM-4 bioactivation. This study characterized biodegradable plastic membranes using test parameters, namely the SEM, FTIR, Swelling, and tensile strength tests. The biodegradation test was carried out by immersing biodegradable plastic membranes in an EM-4 bioactator for 7 days. Based on the results obtained from the characterization of biodegradable plastic membranes, namely the swelling test with concentrations of 5:3, 7:3, and 9:3, with yield values ​​of 62.9921%, 69.8902%, and 71.4286%. The SEM test obtained the best results at a concentration of 7:3. FTIR test on biodegradable plastic membranes containing hydroxyl (O-H) and carbonyl (C-O) groups. The tensile test obtained optimum results at the concentration of biodegradable plastic membranes, namely 9:3. As for the biodegradation test that has been carried out, the start time for degradation is 3 days. BRIN Publishing 2024-07-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/3080 10.55981/jbbi.2024.3080 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 9-19 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/3080/4242 Copyright (c) 2024 Pande Putu Indira Prima Dewi, I Gede Widhiantara, I Made Gde Sudyadnyana Sandhika, Putu Angga Wiradana, Raden Joko Kuncoroningrat Susilo https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/3086 2024-11-01T16:20:34Z JBBI:ART

THE EFFECT OF THE HEALTH CARE INFECTION CONTROL COMMITTEE IN PATIENTS WITH URINARY CATHETERS ON COST CONTAINMENT Amirah, Asriwati Fitriani, Arifah Devi Suarayasa, Ketut Suharto, Teguh Asepty, M.Rizky Priyanka CAUTI Cost Containment HICPAC The high prevalence of healthcare-associated infections threatens hospital services. Urinary tract infections associated with the use of catheters could reach 0.2–4.8 per 1000 catheters/day. In response to this, the Centers for Disease Control and Prevention recommends using the Healthcare Infection Control Practices Advisory Committee (HICPAC) to reduce the incidence of infection in hospitals. To determine the effect of implementing HICPAC on reducing hospital costs for patients with urinary catheters at Delia Hospital, Langkat Regency, in 2019. At the Delia General Hospital in Langkat Regency, Malaysia, quantitative research using a quasi-experimental technique was carried out, in which all patients with urinary catheters were included as respondents. The paired t-test was used to evaluate the data using SPSS software. The results revealed a significant effect of the HICPAC application on the incidence of Catheter-Associated Urinary Tract Infection (CAUTI) in patients during treatment (p = 0.08). The HICPAC method can also reduce the risk of CAUTI by 3.902 times. This study did not evaluate the morbidity and mortality attributable to CAUTI. An assessment of the length of stay, cost analysis, and antibiotic resistance is needed to assess the benefits of the HICPAC guidelines. The use of HICPAC in patients with urinary catheters can reduce the cost of treatment at Delia Hospital, Langkat Regency. BRIN Publishing 2024-10-02 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/3086 10.55981/jbbi.2024.3086 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 87-94 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/3086/6204 Copyright (c) 2024 Asriwati Amirah, Arifah Devi Fitriani, Ketut Suarayasa, Teguh Suharto, M.Rizky Priyanka Asepty https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/3097 2024-01-15T15:17:26Z JBBI:ART

CONCEPTION RATE OF ARTIFICIAL INSEMINATION OF BALI CATTLE ON DIFFERENT SEMEN DEPOSITIONS Firmiaty, Sri Idrus, Muhammad Amiluddin, Amiluddin Sudirman, Sudirman Sonjaya, Herry Suyadi, Suyadi Iskandar, Hikmayani Artificial insemination Bali cattle Conception rate Semen deposition Service per conception This study aims to analyze the pregnancy rate resulting from artificial insemination of Bali cattle with different semen depositions. The research method used was a field experiment with three treatments: semen deposition at positions 3, 4, and 4+ (right side/dexter side). Pregnancy rate data were evaluated through Non-Return Rate (NRR1 and NRR2), Service per Conception (S/C), and Conception Rate (CR). The study was conducted on 120 Bali cattle in Bulukumba District, South Sulawesi. The results showed an increase in NRR in semen deposition 3, 4, and 4+ during the observation period of NRR 31 – 60. Although some breeders reported arousal symptoms late, the increase in NRR indicates a good understanding of arousal detection. Semen deposition at position 3 produces the lowest NRR, which can be caused by reproductive function abnormalities, silent heat, endometritis, or late reporting by farmers. The results of the S/C evaluation showed the lowest value and the highest of CR at semen deposition 4, which means that the semen deposition was closer to the fertilization site. Semen deposition at positions 4+ on dexter side althrough closer to the fertilization site was lower results of CR than posisition 4, because sperma only toward in right tubafolopii. The effectiveness of AI is significantly impacted by accurately detecting and timing the insemination process based on the ovulation period. This study provides important information regarding AI techniques in Bali cattle that can be used to increase the population and genetic quality of beef cattle in Indonesia. BRIN Publishing 2023-12-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/3097 10.55981/jbbi.2023.3097 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 2 (2023); 368-374 2548-611X 2442-2606 10.55981/jbbi.v10i2 eng https://ejournal.brin.go.id/JBBI/article/view/3097/2152 Copyright (c) 2023 Sri Firmiaty, Muhammad Idrus, Amiluddin Amiluddin, Sudirman Sudirman, Herry Sonjaya, Suyadi Suyadi, Hikmayani Iskandar https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/3112 2024-11-01T16:20:34Z JBBI:ART

EFFECT OF COLCHICINE TREATMENT ON PLANT GROWTH AND FLOWER DEVELOPMENT IN Zinnia elegans Kusumawati, Eni Haryanto, Haryanto Wijaya, Nur Iman Alaudin, Ahmad Muzaki Nurrah-man Nurhayati, Sari Fitri, Fadillya Maulidilla Zinnia elegans Refugia Colchicine Growth Development Refugia is a flowering plant that is intended to trap and destroy insects. Colchicine is reported to induce polyploidy in plants. This study aims to determine germination, growth, and refugia performance of colchicine induced Zinnia elegans at different immersion concentrations and times. This research was conducted experimentally with a completely randomized design. The treatment was immersion in colchicine solution at different times duration. Colchicine concentration 0.01% with immersion time of 6,12,24,48, and 72 hours. Colchicine induction with different immersion times showed a decrease in sprouts height (p<0.05), resulting in abnormal sprouts with three leaves and wider leaf area (correlation test r=0.560). The longer the colchicine immersion showed plant height (correlation test r=0.618). Morphologically, control and colchicine induction results were relatively the same, but flowers resulting from colchicine induction had higher flower heights (correlation test r=0.772) and flower diameter (correlation test r=0.815). Induction of colchicine immersion had a different effect on the growth and reproduction performance of Z. elegans. BRIN Publishing 2024-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/3112 10.55981/jbbi.2024.3112 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 43-52 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/3112/4480 Copyright (c) 2024 Eni Kusumawati, Haryanto Haryanto, Nur Iman Wijaya, Ahmad Muzaki Nurrah-man Alaudin, Sari Nurhayati, Fadillya Maulidilla Fitri https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/3123 2024-01-12T15:40:55Z JBBI:ART

EFFICIENT REGENERATION LOCAL BANANA TISSUE CULTURE USING FLORAL APICES BY CYTOKININ COMBINATION Makhsunah, Isnina Tasya Habibi, Ali Mukit Khofifa, Rendryana Aulia Nur Ubaidillah, Mohammad banana floral apices BAP and TDZ Banana (Musa sp.) is an important crop that is widely cultivated and consumed in Indonesia. Banana plants production can be increased through tissue culture with the use of explants and the right combination of hormones. This study was conducted to determine the use of flower apices explants on local banana varieties using a combination of cytokinin hormones containing BAP (Benzylaminopurine) and several levels of TDZ (Thidiazuron). The result of this research was analyzed using a one-way analysis of variance. The hormone combination in this study consists of MS + BAP 2 mg/L + three concentrations of TDZ (2 mg/L, 2.5 mg/L, and 3 mg/L) as a regeneration medium. Three local banana varieties (Kepok Putih, Kepok Kuning, and Pisang Susu) was cultured on the three combination medium. The results showed that the use of flower apices explants contained in banana flowers grown on MS media with a combination of cytokinin hormones could increase the efficiency of explant regeneration. Media MS + BAP 2 mg/L + TDZ 3 mg/L gave the best regeneration rate for Kepok Putih (62.50%), Kepok Kuning (62.50%) and Banana Susu (56.25%) compared to TDZ 2. mg/L and TDZ 3 mg/L. The novelty of this study can provide information in the propagation of local banana plants using flower apices explants. BRIN Publishing 2023-12-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/3123 10.55981/jbbi.2023.3123 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 10 No. 2 (2023); 355-360 2548-611X 2442-2606 10.55981/jbbi.v10i2 eng https://ejournal.brin.go.id/JBBI/article/view/3123/2150 Copyright (c) 2023 Isnina Tasya Makhsunah, Ali Mukit Habibi, Rendryana Aulia Nur Khofifa, Mohammad Ubaidillah https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/3736 2024-11-01T16:20:34Z JBBI:ART

POTENTIAL OF ENCAPSULATION Bacillus cereus BTH-22 AGAINTS BACTERIAL WILT DISEASE ON EGGPLANT: - Purnawati, Arika Triwahyu P, Endang Fari A, Haidar endophytic bacteria, formulation, bacterial wilt, biological control Endophytic bacteria are a group of bacteria that live in plant tissues, potentially as biological agents of plant diseases, especially wilt disease caused by Ralstonia solanacearum and as an inducer of eggplant (Solanum melongena) plant growth. One of these bacteria is Bacillus cereus BTH-22 which was isolated from healthy eggplant stems in Kediri. The purpose and formulation of the problem are to obtain an encapsulation formulation of B. cereus BTH-22 which has the potential as a biological agent against wilt disease caused by R. solanacearum and as an inducer of eggplant (S. melongena) plant growth. The novelty of the research is the encapsulation formulation of B. cereus which is applied to eggplant plants with wilt, because the application generally uses root soaking, watering to the soil surface, mixing with fertilizer, liquid and powder formulations but all are easily washed away by rain. The research method is as follows: (1) Making encapsulation formulation: 75% Na-alginate with 10% aloe vera extract (Na-1) and 75% Na-alginate, 0.875% zeolite and 0.875% sago flour (Na-2), (2) Sterile soil, inoculate R. solanacearum and prepare 1 month old plants, (3) Application of encapsulation formulation was carried out 3 days after inoculation of R. solanacearum (107CFU/mL) using a Completely Randomized Design with 3 replications. Observations were made on days 7, 14, 21, 28, 35, 42, 49 including : disease intensity, plant height, number of leaves, number of flowers. The results of the study showed: viability in the Na-1 and Na-2 treatments was higher than control at the 48th to 96th hours, disease intensity in the Na-1 and Na-2 treatments was lower than control at the 7th to 49th day, plant height in the Na-1 and Na-2 treatments was higher than control at the 14th to 49th day. BRIN Publishing 2024-10-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/3736 10.55981/jbbi.2024.3736 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 155-161 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/3736/6486 Copyright (c) 2024 Arika Purnawati, Endang Triwahyu P, Haidar Fari A https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/3743 2024-11-01T16:20:34Z JBBI:ART

KIDNEY MICROANATOMY OF WHITE RATS ON THE ADMINISTRATION OF ETHANOL EXTRACT OF Spatholobus littoralis STEM ACUTE DOSE Rousdy, Diah Azkiya, Tiara Wardoyo, Elvi Rusmiyanto Pancaning Acute dose kidney microanatomy Spatholobus littoralis secondary metabolite Tampala bajakah stem (Spatholobus littoralis Hassk) is empirically used by the Indonesian people to recover from disease and maintain health, but the use of tampala bajakah has not been tested for doses that are safe for consumption. The purpose of this study was to determine the type of damage caused by acute doses of bajakah tampala stem extract on white rat kidney microanatomy (Rattus norvegicus Berkenhout). This study used bajakah stem derived from Ambawang, extraction using ethanol solvent, and Wistar strain white rat (± 150g) test as a test animal. The method used a Complete Randomized Design with 4 treatments namely normal control using distilled water, treatment of doses of 300, 2000, and 5000 mg/kg BW of bajakah tampala stem extract. Each group was given 5 replicates. The extract was shown in a single dose orally and observations were made for 14 days. The results obtained that doses of 2000 and 5000 mg/kg BW caused damage to white rat kidney tissue in the form of microanatomy dilatation of tubules and loss of the brush border, and doses of 300, 2000, and 5000 mg/kg BW caused necrosis and haemorrhage. The most severe damage to kidney microanatomy is the dose of 2000 and 5000 mg/kg BW. The administration of acute doses of bajakah extract has the potential to cause damage to kidney tissue. BRIN Publishing 2024-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/3743 10.55981/jbbi.2024.3743 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 53-60 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/3743/4481 Copyright (c) 2024 Diah Rousdy, Tiara Azkiya, Elvi Rusmiyanto Pancaning Wardoyo https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/3809 2024-11-01T16:20:34Z JBBI:ART

OPTIMIZING AMOUNT AND IDENTIFICATION OF YEAST IN SALAK YEAST WATER (Salacca edulis Reinw cv Pondoh) Khumaira, Annisa Annaziha, Salma Baihaqi, Muhammad Azizan Azmani Anindita, Nosa Septiana Bimantara, Arif Probowati, Wiwit fruit yeast water identification optimization salak yeast Salak fruit is a tropical fruit spread across Southeast Asia and can potentially be a source of natural yeast by processing fruit yeast water. Salak fruit is known to be rich in carbohydrates, making it a potential natural habitat for microbial communities, especially yeast. This research aims to optimize the growth of yeast in the salak yeast water system by varying the sugar concentration treatment, the composition of the amount of fruit flesh, and the fermentation time, as well as identifying the yeast in the salak yeast water. The method used is to count the number of yeast colonies using the Total Plate Count method. The most optimal treatment results are then tested for pH, total sugar, alcohol, isolation and yeast identification. The research results showed that the composition of salak meat was 30%, adding 1% sugar, and fermentation for 6 days resulted in the most optimal growth of the microbial community with an amount of 3.1×10⁶ cfu/ml. The pH test showed a result of 3.01, the alcohol content, namely ethanol, was 0.066855%, no methanol was detected, the total sugar test result in the yeast water was 2.08%, and it was identified in the yeast water that there were Hanseniaspora opuntiae and Candida sorboxylosa. The results show that salak yeast water (Salacca edulis Reinw cv Pondoh) can be used as a yeast water product to ferment food. BRIN Publishing 2024-07-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/3809 10.55981/jbbi.2024.3809 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 20-32 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/3809/4243 Copyright (c) 2024 Annisa Khumaira, Salma Annaziha, Muhammad Azizan Azmani Baihaqi, Nosa Septiana Anindita, Arif Bimantara, Wiwit Probowati https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/4845 2024-11-01T16:20:34Z JBBI:ART

THE SORUS MORPHOLOGY OF FERNS OF THE FAMILY Dryopteridaceae AND THE FAMILY Polypodiaceae THE TATANGGE UNIVERSITY FOREST, SSOTHEAST SULAWESI Munir, Asmawati Damhuri Samai, Suarna Lisdayanti, Hikma Lizawarni Dryopteridaceae and Polypodiaceae families Sorus morphology Pteridophyta This study aims to determine the morphology of the sorus of ferns (Pteridophyta) Dryopteridaceae and Polypodiaceae families in the Tatangge Education Forest area of Rawa Aopa Watumohai National Park, Southeast Sulawesi. This research involved exploration in the Tatangge University Forest area of Rawa Aopa Watumohai National Park, Southeast Sulawesi to collect samples, then identifying and observing sorus characteristics in the Laboratory of the Biology Education Department, Faculty of Teacher Training and Education, Halu Oleo University, Kendari. The research data were processed and analysed descriptively, regarding the characteristics of the sorus of ferns (Pteridophyta) Dryopteridaceae family including location, shape, colour, and annulus. The results of the study found 5 (five) species of ferns (Pteridophyta) Dryopteridaceae family, and 5 (five) species of Polypodiaceae family that have different sorus characteristics. The Dryopteridaceae family consists of the species Dryopteris celsa, Dryopteris filix-mas, Dryopteris marginalis, Nephrolepis biserrata (Sw) Schoot, and Polistichopsis hasseltii. The family Polypodiaceae consists of the species Drynaria sparsisora (Desv.) T. Moore Pyrrosia longifollia (Burm. f), Pyrrosia lanceolata L Farw, Pyrrosia piloselloides M.G. Price, and Polypodium glycyrriza Licorice Fern. BRIN Publishing 2024-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/4845 10.55981/jbbi.2024.4845 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 1-8 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/4845/4077 Copyright (c) 2024 Asmawati Munir, Damhuri, Suarna Samai, Hikma Lisdayanti, Lizawarni https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/4870 2025-01-16T04:07:00Z JBBI:ART

TRANSDERMAL PATCH PILIS FROM TURMERIC RHIZOME AND BLACK SEEDS FOR TREATMENT BASED ON ETHNOPHARMACY Susanto, Nindita Clourisa Amaris Sawitri, Arinda Handiyah Muslimah, Intan blackseed flavonoid patch transdermal pilis turmeric Pilis, a traditional product containing various herbal ingredients, is currently less desir-able due to its impractical and unfashionable method of application. Pilis is used to treat dizziness, pain, eye fatigue, encephalitis in children, fever, and to aid recovery for mothers after childbirth. However, pilis also carries risks related to uncontrolled dosage and contamination, particularly from chemical or heavy metal pollutants, which may cause toxic reactions. To mitigate these risks, pilis should be used in recommended doses and produced under clean and safe conditions. This research aimed to provide the innovation of patches as an alternative treatment to increase attractiveness and control the active substances released and delivered for a long time. Transdermal patches are made from a combination of turmeric and black seed containing flavonoid active metabolite compounds, namely curcumin, carotenoids, quercetin, kaempferol, and apigenin. The combination has a synergistic effect that can increase total flavonoid levels and the therapeutic effect of pilis. The method used in this research was litera-ture review and data analysis. The results showed the effect of transdermal patch pilis from the combined extracts added to the formula in vitro and in vivo using animal tests. In conclusion, transdermal patch pilis has prospects as a topical treatment based on the combination of an active delivery system with a patch and metered dose system. BRIN Publishing 2024-11-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/4870 10.55981/jbbi.2024.4870 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 2 (2024); 290-302 2548-611X 2442-2606 10.55981/jbbi.v11i2 eng https://ejournal.brin.go.id/JBBI/article/view/4870/6903 Copyright (c) 2024 Nindita Clourisa Amaris Susanto, Arinda Handiyah Sawitri, Intan Muslimah https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/4968 2024-11-01T16:20:34Z JBBI:ART

THE EFFECT OF LONG SOAKING OF SEEDS IN KNO3 SOLUTION AND VARIATIONS IN PLANTING MEDIA ON THE GERMINATION OF CIPLUKAN (Physalis angulata L.) SEEDS IN VITRO hartati, Puji Hastuti, Endah Dwi Setiari, Nintya Nurchayati, Yulita Ciplukan KNO3 Sprouts The Physalis angulata L. (ciplukan), originating from the Solanaceae family, contains secondary metabolites that can be used as medicinal materials. The provision of Physalis angulata seeds is hindered by seed dormancy. Dormancy can be broken by employing a 0.2% w/v KNO3 solution with a specific soaking period. This study aims to optimise the duration of the soaking period for Physalis angulata seed germination and the in vitro growing medium. The seeds were soaked in a 0.2% w/v KNO3 solution for 6 hours, 12 hours, and 24 hours. The soaked seeds were planted in sterile tissue media moistened with Murashige and Skoog (MS) medium solution, a 0.2% w/v KNO3 solution, and distilled water. Generally, the dormancy of Physalis angulata seeds can be broken by soaking them in a 0.2% w/v KNO3 solution. The results of this study indicated that Physalis angulata seeds soaked in a 0.2% w/v KNO3 solution for 6 hours and 12 hours, then planted in sterile tissue media moistened with the MS medium solution, exhibited an optimal germination response. BRIN Publishing 2024-10-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/4968 10.55981/jbbi.2024.4968 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 138-144 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/4968/6426 Copyright (c) 2024 Puji hartati, Endah Dwi Hastuti, Nintya Setiari, Yulita Nurchayati https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/5547 2024-11-01T16:20:34Z JBBI:ART

SARS-CoV-2 VARIANTS GENOME ANALYSIS OF INDONESIAN ISOLATES AND THEIR RESPONSES TO AVAILABLE VACCINES Audah, Kholis Abdurachim Shaikho, Nasser Mohamed Ghassan Mohamed Adnan Yurico, Dyana Jeremy, Michael Anisa, Amalda Siti GISAID.org Indonesia multiple mutations SARS-CoV-2 virus SARS-CoV-2 is a virus that initially appeared in Wuhan, China, at the end of 2019. In Indonesia, multiple variants of SARS-CoV-2 have been identified, as well as various local variants that are not yet considered to be ‘variants of concern’. Therefore, this investigation is intended to understand the prevalence and epidemiology of the virus, along with detecting the mutations that occur in genes associated with whole-genome-sequences (WGS) isolated in Indonesia. Analyses were performed to investigate SARS-CoV-2 prevalence in Indonesia using data obtained from GISAID.org. Whole-genome sequencing was performed on random samples taken from GISAID.org utilizing the BLAST tool from NCBI. The variants identified in Indonesia are alpha, beta and delta variants, as well as local variants B.1.470 and B.1.466.2. In total there are 9,260 isolated genomes collected in GISAID were located in Indonesia. Using BLAST, the variants were compared with the Wild-Type from Wuhan NC.045512.2. Multiple mutations were observed in the samples. The results from whole-genome sequencing of variants isolated in Indonesia have found that multiple mutations have occurred in genes of the SARS-CoV-2 virus and it caused alterations in the characteristics of the virus and may affect vaccine efficacy. BRIN Publishing 2024-10-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/5547 10.55981/jbbi.2024.5547 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 95-105 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/5547/6292 Copyright (c) 2024 Kholis Abdurachim Audah, Nasser Mohamed Ghassan Mohamed Adnan Shaikho, Dyana Yurico, Michael Jeremy, Amalda Siti Anisa https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/5554 2024-11-01T16:20:34Z JBBI:ART

OPTIMIZATION OF METHANE PRODUCTION FROM MIXED SUBSTRATES OF COW FAECES AND CARICA SEEDS USING RESPOND SURFACE METHODOLOGY Sutaryo, Sutaryo Susanto, Setio Purnomoadi, Agung Abeng, Doni Purwasih, Rita Biogas Carica Seeds Dairy Cow Faeces Germination Substrate Anaerobic digestion (AD) of dairy cow feces (DCF) has low methane production per ton of waste. A strategy to overcome this drawback is to co-digest DCF and carica seed (CS). Currently, CS is still a waste from the candied carica in the syrup industry and is often just thrown away into the environment. This research aims to evaluate the optimal level of combination for methane production from DCF with co-substrates of germinated (CGM) and non-germinated (CNG) CS meal using Respond Surface Methodology (RSM). This research uses a completely randomized design with a factorial pattern consisting of the first factor being CGM and CNG, and the second factor being the combined level of DCF and CS (CGM or CNG. The result showed that utilization of CS can increase significantly (p<0.05) methane production of the final substrate compared to the control (digester treating DCF only). Utilization of CGM as co-substrate with DCF can also increase methane production (p<0.05) compared to CNG. All parameters in the liquid phase were in the normal range for AD. Based on the research results, the optimum point with a desirability value close to 1 was achieved at a ratio of CGM10 and DCF90. CS was proven can be used as a co-substrate with DCF to increase methane production of the final substrate and germination can be used as a method to increase the methane yield of CS.  BRIN Publishing 2024-09-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/5554 10.55981/jbbi.2024.5554 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 61-73 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/5554/6202 Copyright (c) 2024 Sutaryo Sutaryo, Setio Susanto, Agung Purnomoadi, Doni Abeng, Rita Purwasih https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/5571 2025-01-16T06:11:13Z JBBI:ART

CONCOMITANT PSORIASIS VULGARIS AND METABOLIC SYNDROME: A CASE REPORT Khairunnisa, Qanita metabolic syndrome proinflammatory cytokines psoriasis Among the various dermatological conditions, psoriasis assumes significant relevance concerning Metabolic Syndrome (MetS). A 57-year-old man presents with pruritic and scaly erythematous plaque on the right ankle persisting for four weeks. The dermatological examination uncovered a noticeable erythematous plaque with a scaly surface on the right ankle, conclusively observed as psoriasis clinical features. Physical examination for metabolic syndrome indicated blood pressure readings of 160/100 mmHg and central obesity with a waist circumference of 98 cm. The psoriasis treatment involves the use of topical corticosteroids and emollients, along with oral metabolic medication consisting of simvastatin and amlodipine, and lifestyle modifications to treat the metabolic disorder. Metabolic syndrome leads to an elevation in the synthesis of pro-inflammatory cytokines, which are shared characteristics with psoriasis. Following comprehensive therapy of ten days of topical treatment and lifestyle modifications, the patient experienced an improvement in the active psoriatic lesions, despite the presence of metabolic disorders. BRIN Publishing 2024-12-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/5571 10.55981/jbbi.2024.5571 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 2 (2024); 412-417 2548-611X 2442-2606 10.55981/jbbi.v11i2 eng https://ejournal.brin.go.id/JBBI/article/view/5571/6997 Copyright (c) 2024 Qanita Khairunnisa https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/5597 2024-11-01T16:20:34Z JBBI:ART

DIVERSITY AND DENSITY OF MEGABENTHOS IN CORAL REEF ECOSYSTEMS IN KERA ISLAND WATERS, KUPANG DISTRICT, INDONESIA Kangkan, Alexander L. Sine, Kiik G. Boik, Rini I. Coral Reefs Density Diversity Index Kera Island Megabenthos Kera Island belongs to the small islands of the East Nusa Tenggara with the potential conservation of coral reefs and various associated megabenthos organisms. This research aims to a) determine the diversity and density of megabenthos in coral reef ecosystems in Kera Island Waters, b) investigate the grouping between variables from environmental characteristics of megabenthos in coral reef ecosystems in Kera Island Waters, and c) observe the relationship between the diversity and density of megabenthos with the percentage of coral cover in Kera Island Waters. The research method used was a survey with direct measurements in the field. Megabenthos data was taken using the Benthos Belt Transect technique, while the coral cover was taken using the Underwater Photo Transect technique and analyzed using CPCe 4.1 software. The research data were then analyzed for density and percentage of megabenthos, diversity index value, (H’) Shannon, coral cover, organic matter, carbonate content, principal component analysis, and correlation. The study's results regarding the diversity index value of megabenthos in Kera Island Waters ranged from 0.796 to 1.631. The similarity of environmental parameters makes two main components. The first includes current velocity, organic content, dissolved oxygen, and depth; the second are carbonate content, TDS, temperature, and salinity characterize the second main component. The average organic matter was 8.32%, while the carbonate content was 50.09%. The coral reefs and megabenthos density had a robust relationship, with a correlation coefficient of 0.93. BRIN Publishing 2024-09-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/5597 10.55981/jbbi.2024.5597 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 74-86 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/5597/6203 Copyright (c) 2024 Alexander L. Kangkan, Kiik G. Sine, Rini I. Boik https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/5751 2024-11-01T16:20:34Z JBBI:ART

TEMPERATURE EFFECTS ON THE STABILITY OF MYCOBACTERIUM TUBERCULO-SIS SHIKIMATE KINASE (MtSK): STRATEGIES FOR SECURE TRANSPORT Wibowo, Aji Komariyah, Tinta Prabandari, Erwahyuni Endang Ariyani, Titin Siska, Eka Glycerol MtSK Stability Temperatures Transportation Mycobacterium tuberculosis Shikimate Kinase (MtSK) has a crucial role in the shikimic pathway, which is essential for this bacteria but is absent in humans, making it a potential target for novel anti-tuberculosis drugs. This study used enzyme-coupled fluorescence to examine the stability of MtSK stored in 50% glycerol at -30 ℃, 4 ℃, and ±28 ℃ for six days. Results showed stable enzyme activity values (α=0.05) at all temperatures. This research underscores that MtSK’s stability depends on its molecular properties, including GC content, hydrophobic residues, Mg2+ binding, and intra-helical salt bridge. Despite some activity decline over time due to glycerol-induced aggregation, MtSK can be safely transported at ±28 ℃ for up to six days without special cooling compartment. Understanding MtSK stability ensures its active conformation remains consistent, reducing off-target effects on drug design and enhancing drug efficacy. This insight ultimately leads to high-quality and commercially viable tuberculosis treatment development. Future research should explore MtSK stability at higher temperatures and assess the optimal glycerol content for cryopreservation. BRIN Publishing 2024-10-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/5751 10.55981/jbbi.2024.5751 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 106-120 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/5751/6293 Copyright (c) 2024 Aji Wibowo, Tinta Komariyah, Erwahyuni Endang Prabandari, Titin Ariyani, Eka Siska https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/5756 2025-01-16T04:07:43Z JBBI:ART

COMBINED TREATMENT OF LANDFILL LEACHATE USING COAGULATION-FLOCCULATION AND ANAEROBIC-AEROBIC BIOFILTER WITH BIOBALL Sugito, Sugito Ratnawati, Rhenny Laba, Vinsensius Fereri Bioball Biofilter Coagulation Flocculation Landfill leachate Piles of waste in landfills can produce leachate that smells bad and contains organic and inorganic materials as well as some pathogenic bacteria. A biofilter is a simple method that can be used to degrade pollutant parameters dissolved in leachate. This research aims to determine the efficiency value of Biological Oxygen Demand (BOD), Chemical Oxygen Demand (COD), and Total Suspended Solid (TSS) concentrations in the leachate of Blandongan Landfill, Pasuruan City. The leachate treatment process uses the coagulation method as pretreatment with aluminum sulfate coagulants (Al2(SO4)3) and Ferric Chloride (FeCl3) followed by biofilter using bioball media. The variables in this research were the differences in the concentration of the coagulant used and the bioball media with a height of 15 cm. The results show that in bioball me-dia the value of reduction efficiency is better in reducing COD levels by 77.73%, BOD by 79.40%, and TSS by 81.48%. This research combines leachate treatment technol-ogy, where the coagulation-flocculation method becomes the pre-treatment of the leachate before continuing with an anaerobic-aerobic biofilter. BRIN Publishing 2024-12-21 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/5756 10.55981/jbbi.2024.5756 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 2 (2024); 366-375 2548-611X 2442-2606 10.55981/jbbi.v11i2 eng https://ejournal.brin.go.id/JBBI/article/view/5756/6957 Copyright (c) 2024 Sugito Sugito, Rhenny Ratnawati, Vinsensius Fereri Laba https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/5807 2024-11-01T16:20:34Z JBBI:ART

LEYDIG CELL COUNT IS INCREASES IN OLD WISTAR RATS (RATTUS NORVEGICUS) BY ANTIOXIDANT ETHANOL EXTRACT OF MORINGA OLEIFERA (EEMO) Widiastini, Luh Putu Karuniadi, I Gusti Agung Manik Moringa Leaf Ethanol Extract Leydig cells White Rat Oxidative stress is the result of an imbalance between antioxidant production and the production of reactive oxygen species (ROS). Leydig cells have a high content of PUFAs in their cell membrane, making it easy to bind ROS. Most natural antioxidants are found in plants, including Moringa. In this work, aged Wistar rats' Leydig cells will be used to test the antioxidant activity of an ethanol extract from the leaves of Moringa oleivera (Rattus norvegicus). The study involved the division of 36 elderly rats, who were between the ages of 18 and 19 months, into two groups. For thirty days, the control group received the same quantity of 0.5% CMC every day, whereas the treatment group received 50 mg/kgBW/0.5 mL of Moringa leaf ethanol extract daily. The rats were healthy and free of physical impairments. The Independent Samples T-Test is used to assess the data in order to find any variations between the treatment and control groups. The significant difference (p < 0.00) in Leydig cell counts between the extract-treated group and the control group suggests that the ethanol extract of Moringa leaves can have a major effect on the number of Leydig cells in Old Wistar rats (Rattus Norvegicus). BRIN Publishing 2024-10-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/5807 10.55981/jbbi.2024.5807 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 162-169 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/5807/6558 Copyright (c) 2024 Luh Putu Widiastini, I Gusti Agung Manik Karuniadi https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/5966 2025-01-16T04:05:39Z JBBI:ART

MICROBIAL CONTAMINATION AND BIOACTIVE COMPOUNDS OF JAMU BERAS KENCUR Gunardi, Wani Devita Teiseran, Virginia Marsella Timotius, Kris Herawan Flavonoid GC-MS Jamu Beras Kencur Kaempferia galangal LC-MS/MS Background: Jamu Beras Kencur (JBK) is recognized as an herbal medicine, containing two main components: the rhizome of Kaempferia galanga and rice. While previous research has identified bioactive compounds in galangal rhizomes, such as Ethyl p-Methoxycinnamate (EPMC), Ethyl-cinnamate, and Kaempferol, there are few reports on polar or aqueous compounds in JBK. LC-MS/MS and GC-MS enable comprehensive analysis of bioactive compounds, with LC-MS/MS detecting non-volatile, polar, and thermally sensitive compounds like flavonoids and glycosides, while GC-MS analyzes volatile and semi-volatile compounds, such as terpenoids, providing precise separation and identification. Therefore, this study were to know the amount and the growth of contaminant bacteria, yeast and mold; to determine the main bioactive compounds in JBK; and to determine the bioactive compound in aqueous and ethanolic extracts of rhizome that analysed with LC-MS/MS and GC-MS. Methode: JBK samples were sourced from local producers in West Jakarta, freshly prepared, and immediately analyzed for microbial contamination and bioactive compounds. Result: The analysis revealed microbial contamination in JBK, including Escherichia coli, Staphylococcus aureus, Coliform, yeast, and mold. Additionally, three novel flavonoid glycosides were identified: Chrysoeriol-4'-O-β-D-glucopyranoside, Patuletin-7-O-[6′′-(2-methylbutyryl)]-glucoside, and Acacetin-7-galactoside. Conclusion: Therefore, from the pharmacological perspective, JBK has the potentials as a healthy herbal drink. However, further preclinical and clinical studies are essential to validate its safety and efficacy for clinical use, which could pave the way for its integration into mainstream healthcare as a natural therapeutic option. BRIN Publishing 2024-11-16 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/5966 10.55981/jbbi.2024.5966 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 2 (2024); 262-277 2548-611X 2442-2606 10.55981/jbbi.v11i2 eng https://ejournal.brin.go.id/JBBI/article/view/5966/6832 Copyright (c) 2024 Wani Devita Gunardi, Virginia Marsella Teiseran, Kris Herawan Timotius https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/6202 2024-11-01T16:20:34Z JBBI:ART

ANTIFUNGAL ACTIVITY OF N-HEXANE EXTRACT FROM Chaetoceros calcitrans AGAINST Candida sp. Rachmawati, Firdha Naully, Patricia Gita Kania, Prina Puspa Pasha, Delia Ayu Antifungal Chaetoceros calcitrans GC-MS Microalgae Skin Infection Skin infections caused by Candida albicans and Candida krusei pose a serious health issue. One major concern regarding these infections is the resistance to antifungal drugs, highlighting the need for natural antifungals. Chaetoceros calcitrans, a microalgae, is known to contain natural antimicrobial compounds. This study aims to evaluate the antifungal potential of C. calcitrans n-hexane extract against both pathogens. The antifungal activity was tested using the diffusion method. The results indicated that the extract at a concentration of 100 mg mL⁻¹ inhibited the growth of C. albicans and C. krusei, showing the highest inhibition zones of 10.3 ± 0.9 mm and 9 ± 1.4 mm, respectively. GC-MS analysis revealed that the C. calcitrans extract contains antifungal compounds, including 2-Butyl-1-hexyloctahydro-1H-indene, at a concentration of 30.72%. Therefore, it can be concluded that C. calcitrans extract possesses antifungal activity and has potential as a drug candidate for fungal skin infections. BRIN Publishing 2024-10-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/6202 10.55981/jbbi.2024.6202 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 145-154 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/6202/6485 Copyright (c) 2024 Firdha Rachmawati, Patricia Gita Naully, Prina Puspa Kania, Delia Ayu Pasha https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/6422 2024-11-01T16:20:34Z JBBI:ART

THE CURRENT STRATEGIES, RECENT PROGRESS AND REMAINING CHALLENGES FOR DEVELOPING MRNA VIRAL VACCINE Irawan, Priscilla Felicia Apriliani Bela, Budiman DC-specific-LNP-targeting mRNA viral vaccine safety translation efficiency viral IRES The mRNA expression system has revolutionised biotechnology, notably in viral mRNA vaccine development, cancer immunotherapy, and gene therapy. However, recent safety concerns regarding the COVID-19 mRNA vaccine have emerged, particularly regarding its rare adverse effects and its possible connection to cancer. This review explains several approaches used in developing viral mRNA vaccines, the past obstacles solved in generating the current COVID-19 mRNA vaccine, and finally the current advancements and ongoing challenges in the viral mRNA vaccine field. We particularly focus on strategies and methods to improve the safety and translation efficiency of the mRNA vaccine, such as enhancing the vaccine’s transfection specificity to targeted dendritic cells (DC) and using viral IRES or self-amplifying mRNA format to improve mRNA translation efficiency. BRIN Publishing 2024-10-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/6422 10.55981/jbbi.2024.6422 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 121-137 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/6422/6422 Copyright (c) 2024 Priscilla Felicia Apriliani Irawan, Budiman Bela https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/6488 2024-11-01T16:20:34Z JBBI:ART

BIOACTIVE METABOLITES OF Lactiplantibacillus plantarum ISOLATED FROM Sonchus arvensis AS AN ANTIMICROBIAL AGENT Syaputri, Yolani Nurhidayat, Fahmi Wulandari, Indri Rahayu, Sri Rejeki Bioactive metabolites Hydrogen peroxide Lactic acid Lpb Plantarum Plantaricin Sonchus arvensis is a plant widely found in Southeast Asia and often used in traditional medicine. Lactiplantibacillus plantarum is a bacteria isolated from S. arvensis and categorized as qualified presumption of safety by the European Food Safety Authority and the US Food and Drug Administration. Therefore, this study aimed to determine the bioactive metabolites of Lpb. plantarum as antimicrobial agents. Lactic acid bacteria (LAB) were isolated using the serial dilution method, followed by isolation and amplification of the DNA through a 16S rRNA universal primer. Antimicrobial activity was screened using the well-diffusion method. Plantaricin gene identification was performed using PCR and the determination of lactic acid content was conducted using Spectrophotometric. In addition, the titration method was used to measure and determine the hydrogen peroxide. The results showed that Lpb. plantarum had higher inhibition toward pathogen bacteria than Lc. lactis. Lpb. plantarum had the largest inhibition zone against B. subtillis, followed by S. aureus and E. coli, respectively. It was discovered that Lpb. plantarum precipitated with ammonium sulfate had a greater protein content and antibacterial activity. Furthermore, Lpb. plantarum, which encodes plnA and plnEF, produced lactic acid and hydrogen peroxide at concentrations of 3.0158±0.2774 mg/mL and 0.195±0.04 mg/mL, respectively. BRIN Publishing 2024-10-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/6488 10.55981/jbbi.2024.6488 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 170-187 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/6488/6562 Copyright (c) 2024 Yolani Syaputri, Fahmi Nurhidayat, Indri Wulandari, Sri Rejeki Rahayu https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/6583 2025-01-16T04:07:12Z JBBI:ART

THE IN VITRO AND IN VIVO EFFECTS OF Persea Americana ETHANOL EXTRACT AS AN ANTIHYPERTENSIVE AND ANTIOXIDANT IN PREDNISONE-INDUCED RATS Elisa, Novi Dian Advistasari, Yustisia Sari, Wulan Kartika Pratiwi, Mustika Endah Tobi, Claudius Hendraman Boli Murti, Bayu Tri Putri, Athika Darumas Antihypertensive Antioxidants Avocado Cardiovascular Catalase Malondialdehyde Hypertension impacts the quantity of free radicals within the body. This study aimed to investigate the effect of antihypertension based on free radical levels in the predni-sone-induced rats, focusing on evaluating specific anti-hypertensive fraction of avoca-do leaves. The study's methods conducting GC-MS, anti-hypertensive, and assessing malondialdehyde levels and catalase enzyme. Systolic blood pressure measurements indicated that the positive control (131 ± 3 mmHg) and ethyl acetate fraction (136 ± 4 mmHg) were statistically similar, significantly different from the negative control group. Diastolic blood pressure measurements showed a blood pressure decreasing in posi-tive control (105 ± 7) that similar statistically with ethyl acetate fraction (104 ± 6), and significantly difference with negative control group. Malondialdehyde levels were nota-bly elevated in both the positive control (37.79 ± 5.47) and ethyl acetate fraction (38.01 ± 5.47) compared to the negative control (-9.07 ± 10.10), indicating increased free rad-ical. Catalase activity demonstrated significant differences, with the positive control (44.06 ± 5.44) and ethyl acetate fraction (44.05 ± 5.45) showing similar levels, both substantially higher than the negative control (13.90 ± 21.50). In summary, this study indentificates several flavonoid compounds that promising anti-hypertension effect from ethyl acetate fraction of avocado leaves extract. BRIN Publishing 2024-12-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/6583 10.55981/jbbi.2024.6583 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 2 (2024); 313-324 2548-611X 2442-2606 10.55981/jbbi.v11i2 eng https://ejournal.brin.go.id/JBBI/article/view/6583/6952 Copyright (c) 2024 Novi Elisa, Yustisia Dian Advistasari, Wulan Kartika Sari; Mustika Endah Pratiwi, Claudius Hendraman Boli Tobi, Bayu Tri Murti, Athika Darumas Putri https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/7120 2024-11-01T16:39:11Z JBBI:ART

MORPHOLOGICAL INDICES: DISTINGUISHING SINGLE AND TRIPLETS-BEARING BOER AND ETAWA GRADE GOATS IN LOMBOK BARAT Fitriyah, Abyadul Mariani, Yuni Kartika, Ni Made Andry Fajri, Nefi Andriana Alimuddin Harmayani, Ria Boer goat Etawah goat Morphological indices Single-Bearing Triplet-Bearing This study aimed to identify female goats with the potential to give birth to triplets by examining morphological characteristics correlated with FSH and LH hormone levels in the blood. It included four triplet-bearing Boer goats and four triplet-bearing Etawah grade goats, each with 12 kids, and four single-bearing Boer goats and four single-bearing Etawah grade goats, each with four kids. Morphological indices of body sizes, including weight, height slope, length index, width slope, depth index, foreleg length, and hormone levels, were observed. A t-test and descriptive analysis were conducted. The average body size of Boer goats was larger than Etawah grade goats, but there were no significant morphological differences between triplet-bearing Boer and Etawah grade goats. However, single-bearing Boer goats had a significantly higher height slope than single-bearing Etawah grade goats. FSH levels significantly increased in the blood of triplet-bearing Boer goats compared to single-bearing Boer goats. Morphological indices and hormone levels can help select goats likely to give birth to triplets. BRIN Publishing 2024-10-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/7120 10.55981/jbbi.2024.7120 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 1 (2024); 199-222 2548-611X 2442-2606 10.55981/jbbi.v11i1 eng https://ejournal.brin.go.id/JBBI/article/view/7120/6617 Copyright (c) 2024 Abyadul Fitriyah, Yuni Mariani, Ni Made Andry Kartika, Nefi Andriana Fajri, Alimuddin, Ria Harmayani https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/7431 2025-01-16T04:08:02Z JBBI:ART

SCALE-UP OF PHENOLIC COMPOUNDS PRODUCTION FROM Styrax paralleloneurum LEAVES AND EXTRACT PURIFICATION USING AMBERLITE XAD-2 Martgrita, Merry Meryam Sianipar, Imelda Febrika Manurung, Adelina Amberlite XAD-2 Aspergillus niger ion exchange chromatography Styrax paralelloneurum submerged fermentation The current study aimed to determine the total phenolic compound content, antioxidant activity, and antibacterial activity of fermented Styrax leaf extract in 2000 mL volume. Fermentation by Aspergillus niger was carried out for 64 hours. After extraction, purifi-cation was conducted using Amberlite XAD-2 resin in ion exchange chromatography. The total phenolic content of the unpurified extract was 92.75 mg GAE.g-1, while the purified extract was 146.67 mg GAE.g-1. Antioxidant activity expressed in IC50 values was 31.71 ppm for the unpurified extract and 14.11 ppm for the purified extract. The antibacterial activity tested on Staphylococcus aureus and Pseudomonas aeruginosa showed an inhibition zone diameter of 31.43 mm and 28.2 mm, respectively for the unpurified extract, while for the purified extract 33.96 mm and 35.5 mm, respectively. It can be concluded that the purification of fermented Styrax leaf extract caused a stronger antioxidant activity and a very potent antibacterial activity. BRIN Publishing 2024-12-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/7431 10.55981/jbbi.2024.7431 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 2 (2024); 400-411 2548-611X 2442-2606 10.55981/jbbi.v11i2 eng https://ejournal.brin.go.id/JBBI/article/view/7431/6988 Copyright (c) 2024 Merry Meryam Martgrita, Imelda Febrika Sianipar, Adelina Manurung https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/7739 2025-01-16T04:07:48Z JBBI:ART

UTILIZATION OF OIL PALM EMPTY FRUIT BUNCHES ENHANCED WITH MOLASSES FOR XYLITOL PRODUCTION Clarissa, Clarissa Valerie, Levina Hadi, Alvinsyah Ramdani Pradipta Purnamadjaja, Andrew Medha Valentino, Figo Kembaren, Riahna Oil palm empty fruit bunches Xylitol Moniliella pollinis SP5 Ethyl methanesulfonate Molasses Bio-based product A minimal oil palm empty fruit bunch (OPEFB) usage encourages increased OPEFB utilization to prevent its loss of economic prospects. The xylose and arabinose constituents of OPEFB’s hemicellulose part can be utilized as raw materials for xylitol production. A high diabetes mellitus and periodontal disease prevalence in Indonesia makes xylitol a safe and helpful low-calorie sweetener alternative. This research explores the OPEFB molasses-supplemented media and compares Moniliella pollinis SP5 and other M. pollinis ethyl methanesulfonate (EMS) mutants in xylitol yields. It was found that M. pollinis SP5 accomplished 27% better performance in OPEFB+15%(v/v) molasses than in OPEFB from 0.48 g/g to 0.61 g/g. In addition, M4, M5, and M6 mutants from EMS mutagenesis generated more xylitol concentration in OPEFB + 15%(v/v) molasses compared to the SP5 strain. These findings provide in-sights into the potential of xylitol manufacture with OPEFB. For future research, it is recommended that xylitol production employing OPEFB be optimized. BRIN Publishing 2024-12-21 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/7739 10.55981/jbbi.2024.7739 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 2 (2024); 376-387 2548-611X 2442-2606 10.55981/jbbi.v11i2 eng https://ejournal.brin.go.id/JBBI/article/view/7739/6958 Copyright (c) 2024 Clarissa Clarissa, Levina Valerie, Alvinsyah Ramdani Pradipta Hadi, Andrew Medha Purnamadjaja, Figo Valentino, Riahna Kembaren https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/8156 2025-01-16T04:07:17Z JBBI:ART

PREVENTION OF GASTROPROTECTIVE ACTIVITY OF CHINESE BETEL EXTRACT (PEPEROMIA PELLUCIDA L.) ON THE HISTOPATHOLOGICAL FEATURES OF THE STOMACH IN A MOUSE MODEL OF GASTROENTERITIS Savitri, Lisa Prasetyawan, Fendy Saristiana, Yuneka Juwita, Syntia Tanu Amanda, Cornelia Mebung, Konradus Klala Bioactive compounds Gastroprotective Histopathology Peperomia pelucida L Gastroenteritis is a common gastrointestinal disorder characterized by inflammation of the stomach and small intestine, often leading to symptoms such as diarrhea, vomit-ing, and abdominal pain. This study investigates the gastroprotective effects of Pepe-romia pellucida (Chinese betel) extract on histopathological features of the stomach in a mouse model of gastroenteritis. Male Swiss strain mice, aged 2-3 months, were treated with different doses of P. pellucida extract (100, 300, and 500 mg/kg BW) for 7 days after inducing gastroenteritis with Escherichia coli infection. Histopathological ex-amination showed that the extract significantly reduced cell degeneration, necrosis, and polymorphonuclear leukocyte infiltration in the stomach compared to the negative control group. The highest dose (500 mg/kg BW) exhibited the most prominent gastro-protective effect. These results suggest that P. pellucida possesses promising thera-peutic potential for gastroenteritis, possibly due to its bioactive compounds such as flavonoids, tannins, and saponins. Further studies are needed to explore the underly-ing mechanisms and optimize its therapeutic application. BRIN Publishing 2024-12-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/8156 10.55981/jbbi.2024.8156 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 2 (2024); 325-333 2548-611X 2442-2606 10.55981/jbbi.v11i2 eng https://ejournal.brin.go.id/JBBI/article/view/8156/6953 Copyright (c) 2024 Lisa Savitri, Fendy Prasetyawan, Yuneka Saristiana, Syntia Tanu Juwita, Cornelia Amanda, Konradus Klala Mebung https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/8176 2026-07-21T01:38:17Z JBBI:ART

IN-SILICO ANALYSIS OF THE INTERACTION BETWEEN D7 PROTEIN FROM THE SALIVARY GLAND OF Ae. albopictus AND Thromboxane A2 FOR DEVELOPING ANTIPLATELET AGENT Wathon, Syubbanul Senjarini, Kartika Oktarianti, Rike Lelono, Asmoro antiplatelet D7 protein eicosanoid molecular docking TxA2 The salivary glands of mosquito vector diseases contain various biological components which facilitate blood-feeding into the host's body. These components are mostly protein molecules. Numerous protein molecules in the salivary glands have gained substantial research emphasis to determine their role and function, including those in the salivary glands of Ae. albopictus. D7 protein is the main component in Aedes salivary glands, which aids in inhibiting platelet aggregation by binding to the Thromboxane A2 (TxA2) during the blood-feeding. TxA2 is a eicosanoid molecule that stimulates platelet aggregation. The protein's ability to bind TxA2 shows that this protein has potential as a new antiplatelet agent. The examination of the D7 protein in binding TxA2 was performed through an in-silico approach using the molecular docking method. This research included selecting the 3D model of the D7 protein and the TxA2 ligand, preparing the 3D model of the D7 protein, native ligands, and test ligands, targeted molecular docking method, validating the molecular docking, analysis and visualization of the docking results. The molecular docking validation shows an RMSD value of 1.657 Å. The results of molecular docking show an ΔG value of -5.60 kcal/mol, meaning that the D7 protein can bind to the TxA2 ligand stably and spontaneously. The active site of the D7 protein in binding the TxA2 ligand consists of several amino acid residues, namely THR 190, GLU 268, TYR 178, PHE 154, ILE 175, ARG176, VAL 293, TYR 248, and TYR 178. The ability of D7 protein to bind TxA2 as an inducer of platelet aggregation has demonstrated its potential as a novel antiplatelet agent. These results can pave further development of drug discovery in the medical and pharmaceutical fields. BRIN Publishing 2025-06-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/8176 10.55981/jbbi.2025.8176 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 212-227 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/8176/10155 Copyright (c) 2025 Syubbanul Wathon, Kartika Senjarini, Rike Oktarianti, Asmoro Lelono https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/8192 2025-01-16T04:07:23Z JBBI:ART

UTILIZATION OF PINEAPPLE PROCESSING LIQUID WASTE IN BIODEGRADATION OF DISPOSABLE FACE MASK BY BACTERIA FROM LAMPUNG BAY Deviany, Deviany Zhofiroh, Nawrah Meisyah Muthi'ah Febrina, Yane Fita Yusuf, Reggina Aulia Achmad, Feerzet Yuniarti, Reni Istiadi, Khaerunissa Anbar Biodegradation Pineapple processing liquid waste Disposable face mask Microplastics Lampung bay The designation of COVID-19 as a global pandemic led to an increased use of single-use face masks, which result in waste that is difficult to degrade and has the potential to release microplastic fibers into the environment. This study aims to examine the im-pact of adding pineapple peel liquid waste (LCN) as a growth medium for the biodeg-radation of single-use face masks by bacterial isolates obtained from the waters of the Lampung Bay. The study investigates how LCN affects the efficiency of mask degra-dation by microorganisms, as well as how the ratio of LCN mixed with other growth media, such as Nutrient Broth (NB), influences the degradation process. The biodeg-radation process was carried out using a biostimulation technique, where bacterial iso-lates were incubated in media containing LCN at a 1:1 ratio. The degradation process lasted for 15 days, with the results being analyzed using gravimetry and Fourier-Transform Infrared Spectroscopy (FTIR). Gravimetric results showed a greater weight reduction in treated masks compared to the control masks. FTIR analysis also indicat-ed changes in the intensity of functional groups in the degraded layers of the masks, as well as the emergence of C≡C functional groups in the second and third layers. This study demonstrates that the addition of LCN can accelerate the biodegradation of sin-gle-use face masks, offering a new approach for managing mask waste. BRIN Publishing 2024-12-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/8192 10.55981/jbbi.2024.8192 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 2 (2024); 334-343 2548-611X 2442-2606 10.55981/jbbi.v11i2 eng https://ejournal.brin.go.id/JBBI/article/view/8192/6954 Copyright (c) 2024 Deviany Deviany, Nawrah Meisyah Muthi'ah Zhofiroh, Yane Fita Febrina, Reggina Aulia Yusuf, Feerzet Achmad, Reni Yuniarti, Khaerunissa Anbar Istiadi https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/8235 2025-01-16T04:07:32Z JBBI:ART

THE ANTI-APOPTOTIC POTENTIAL OF Paederia foetida L. LEAF EXTRACT THROUGH THE DOWNREGULATION OF CASPASE-3 EXPRESSION IN AN ESCHERICHIA COLI-INDUCED SEPSIS MICE MODEL Savitri, Lisa Krissanjaya, Rochmad Juwita, Syntia Tanu Sukmawati, Datin An Nisa Probosiwi, Neni Laili, Nur Fahma Ilmi, Tsamrotul Prasetyawan, Fendy Spleen Caspase-3 Sepsis Paederia foetida L. Escherichia coli Sepsis is a life-threatening condition characterized by an abnormal immune response to infection, leading to high mortality rates in intensive care units (ICUs) worldwide. Caspase-3, a crucial enzyme in the apoptosis pathway, plays a significant role in sep-sis-related cellular damage. This study investigates the anti-apoptotic potential of Paederia foetida L. leaf extract by examining its effect on Caspase-3 expression in an Escherichia coli-induced sepsis mice model. Male Balb/c mice were divided into six groups, including positive control, negative control, and treatment groups receiving varying doses of the extract (100 mg/kgBW, 300 mg/kgBW, and 500 mg/kgBW). Caspase-3 expression in the spleen was measured after 24 hours of treatment. The results demonstrated a significant reduction in Caspase-3 expression, particularly in the group treated with 500 mg/kgBW of the extract, indicating its anti-apoptotic effect. These findings suggest that P. foetida leaf extract may serve as a potential therapeutic agent for reducing cell apoptosis in sepsis, warranting further investigation into its mechanisms and clinical pharmacological field. BRIN Publishing 2024-12-21 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/8235 10.55981/jbbi.2024.8235 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 2 (2024); 356-365 2548-611X 2442-2606 10.55981/jbbi.v11i2 eng https://ejournal.brin.go.id/JBBI/article/view/8235/6956 Copyright (c) 2024 Lisa Savitri, Rochmad Krissanjaya, Syntia Tanu Juwita, Datin An Nisa Sukmawati, Neni Probosiwi, Nur Fahma Laili, Tsamrotul Ilmi, Fendy Prasetyawan https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/8237 2025-01-16T04:05:11Z JBBI:ART

UTILIZATION OF FISH PROTEIN HYDROLYSATE AS A NITROGEN SOURCE IN FERMENTED MEDIA OF CEPHALOSPORIN C PRODUCTION BY ACREMONIUM CHRYSOGENUM Biomcc 00141 Fatonah, Ani Fatonah Saepudin, Endang Suyanto Acremonium chrysogenum Biomcc 0141 Antibiotics Cephalosporin C Fermentation Nitrogen Optimizing Indonesia's maritime resources as an effort to reduce reliance on imported raw materials for the pharmaceutical industry. This study investigates the use of fish protein hydrolysate, derived from trash fish flour, as a supplement in the fermentation process for producing cephalosporin C. This key raw material is used to create cephalosporin antibiotics through fermentation with the fungus Acremonium chrysogenum Biomcc 0141. Hydrolysate from trash fish flour is obtained through an enzymatic hydrolysis process using a protease found in pineapple hump extract which was optimized. The results of hydrolysis optimization on hydrolysate from trash fish flour produce a protein content of 3.37%. The protein hydrolysate obtained is then used in the cephalosporin C fermentation process. The addition of trash fish protein hydrolysate to cephalosporin C fermentation media showed a significant increase in the productivity of cephalosporin C, which is up to 85.39% higher than the fermentation process without the addition of fish protein hydrolysates. BRIN Publishing 2024-11-16 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/8237 10.55981/jbbi.2024.8237 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 2 (2024); 236-243 2548-611X 2442-2606 10.55981/jbbi.v11i2 eng https://ejournal.brin.go.id/JBBI/article/view/8237/6830 Copyright (c) 2024 Ani Fatonah Fatonah, Suyanto, Endang Saepudin https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/8521 2025-01-16T04:07:28Z JBBI:ART

IN SILICO ANALYSIS OF SMALL INTERFERING RNA TARGETING THE NUCLEOPROTEIN GENE OF INFLUENZA VIRUSES Hartawan, Risza Ratnawati, Atik Sendow, Indrawati Dharmayanti, Ni Luh Putu Indi siRNA Influenza Nucleoprotein Bioinformatic RNA secondary and tertiary structure Small interfering RNA (siRNA) is a promising therapeutic against viral infection, includ-ing Influenza viruses. However, the Influenza viruses have massive variants with high mutation rates. Therefore, the siRNAs could be futile against newly emerging viruses. Thus, this study aimed to analyze siRNAs targeting the nucleoprotein gene of Influen-za viruses. Using bioinformatic analyses, the siRNAs were simulated against 5 sub-types of Influenza viruses, including H1N1, H3N2, H5N1, H7N9, and H9N2. Bioinfor-matic tools for the folding structure of messenger RNA were utilized to select effective siRNA. As a result, 32 siRNA sequences targeting the nucleoprotein gene were identi-fied. The precision medicine concept seems applied to the siRNA treatment for the In-fluenza virus since each siRNA is effective in its respective virus target. Based on the nucleotide mismatch parameter, most siRNA does not have coverage for the multiple infections of all five subtypes of Influenza viruses, except for NP1089 and NP1496. Later, the secondary and tertiary structure analysis of messenger RNA demonstrated that siRNA has different circumstances in its RNA target position. Therefore, siRNA mapping based on the RNA folding structure approach provides a tool for selecting more effective sequences against Influenza virus infection. Both siRNA NP1089 and NP1496 were predicted to have similar effectivity in knocking down Influenza virus in-fection. Moreover, the cocktail application of siRNA treatment may be effective as an alternative strategy in matching co-infection of multiple Influenza virus subtypes. BRIN Publishing 2024-12-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/8521 10.55981/jbbi.2024.8521 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 2 (2024); 344-355 2548-611X 2442-2606 10.55981/jbbi.v11i2 eng https://ejournal.brin.go.id/JBBI/article/view/8521/6955 Copyright (c) 2024 Risza Hartawan, Atik Ratnawati, Indrawati Sendow, Ni Luh Putu Indi Dharmayanti https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/8591 2025-01-16T04:07:07Z JBBI:ART

UTILIZATION OF KECOMBRANG FRUIT PEEL (Etlingera elatior (Jack) RMSm) AS A MOUTHWASH TO PREVENT MOUTH ULCERS AND DENTAL CARIES Rahmadina, Rahmadina Mayasari, Ulfayani Antimicrobial Dental Carie Kecombrang Bark Mouthwash Thrush Kecombrang fruit is used by the community as a food ingredient but actually the skin of this kecombrang fruit can be used as a mouthwash for the development of dental caries. This study aims to evaluate the preparation of mouthwash formulations with the addition of kecombrang fruit peel extract. The work procedure starts from extraction, phytochemical testing, antimicrobial testing of extracts, and evaluation of mouthwash preparations. The results contained secondary metabolites in the form of alkaloids, fla-vonoids, tannins, and saponins. Evaluation of mouthwash preparation with pH test is 5.0-5.6, organoleptic test which does not change significantly, viscosity test at 20% and 30% concentration is 1.0193 cP and 1.0061 cP, and antimicrobial test with inhibi-tion diameter on Streptococcus mutans and Candida albicans is 11.3 mm and 11.1 mm. In conclusion, mouthwash with added extracts can inhibit the growth of microbes that cause mouth ulcers and tooth decay. BRIN Publishing 2024-11-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/8591 10.55981/jbbi.2024.8591 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 2 (2024); 303-312 2548-611X 2442-2606 10.55981/jbbi.v11i2 eng https://ejournal.brin.go.id/JBBI/article/view/8591/6904 Copyright (c) 2024 Rahmadina Rahmadina, Ulfayani Mayasari https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/8626 2026-01-28T03:39:11Z JBBI:ART

THE ANTIMYCOBACTERIAL POTENTIAL OF SAMBILOTO (Andrographis paniculata Nees) EXTRACT AGAINST Mycobacterium tuberculosis H37Rv WITH Microscopic-Observation AND Drug-Susceptibility (MODS) METHODE Asarini Abdillah, Syamsudin Sani, Yulvian Alam, Gemini Andrographis paniculata Antimycobacterial activity MODS Pharmacokinetic prediction Andrographis paniculata, commonly known as sambiloto, is empirically used for various treatments, including its ability to inhibit the growth of Mycobacterium tuberculosis (M. tuberculosis). This study aims to evaluate the growth inhibition activity of M. tuberculosis strain H37Rv using sambiloto herb extract (Andrographis paniculata). The sambiloto extract was prepared using the maceration method. M. tuberculosis was grown on MODS medium, and the toxicity of sambiloto (A. paniculata) was analyzed using pharmacokinetic prediction studies (pkCMS). The results showed that at concentrations of 0.255 mg/ml, 1.275 mg/ml, and 2.55 mg/ml of sambiloto extract, M. tuberculosis growth occurred on days 7 to 14. Further observations were made until day 28, and it was found that starting at a concentration of 6.375 mg/ml, sambiloto extract did not show M. tuberculosis growth in MODS medium. The positive control, isoniazid, did not show bacterial growth, while the negative control showed extensive bacterial growth on day 12. The determination of M. tuberculosis growth was based on microscopic observations of the bacterial colonies, focusing on cord formation. In conclusion, this study, the use of an extract with a concentration of 6.375 mg/ml in the MODS method showed no growth of Mycobacterium tuberculosis, indicating that the extract is effective in inhibiting bacterial growth at this concentration. The online pkCSM test conducted in this study showed that the extract used is not cytotoxic, meaning that the extract is safe for body cells and does not cause cellular damage. Therefore, it has the potential to be an adjunct therapy in the treatment of tuberculosis. BRIN Publishing 2025-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/8626 10.55981/jbbi.2025.8626 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 1-13 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/8626/8919 Copyright (c) 2025 Asarini, Syamsudin Abdillah, Yulvian Sani, Gemini Alam https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/8938 2025-06-11T14:45:14Z JBBI:ART

THE EFFECT OF AMMONIUM-BASED NPK FERTILIZERS ON THE GROWTH OF MANGROVE SEEDLINGS Rhizopora mucronata AND Rhizopora stylosa Hanindipto, Fasa Aditya Rasima Daulay, Fathin Puji Rahayu, Sarwendah Putri Irsani, Izma Rizki Rachmani, Alfina Evangelina, Helga Mangrove ecosystems Seedling growth Rhizophora mucronate Rhizophora stylosa Biodiversity conservation Wetland ecology Mangroves play an important role in coastal ecosystems, but their growth is often limited by nutrient availability, particularly nitrogen, phosphorus, and potassium (NPK) availability. The use of ammonium-based fertilizers can provide additional benefits by accelerating the growth of mangrove seedlings. This study examines the influence of various doses of ammonium-based NPK fertilizers on the growth of two mangrove species, Rhizophora mucronata and Rhizophora stylosa, The study was carried out in two different places with distinct environmental characteristics, Rhizophora mucronata was planted near land, whereas Rhizophora stylosa was planted in open areas near the sea. The seedlings received dosages of ammonium-based NPK fertilizer. Periodic measurements of growth characteristics, including leaf length, root length, plant height, and SPAD (Soil Plant Analysis Development) index, were conducted and tested statistically. Rhizophora mucronata responded effectively to ammonium-based fertilizer treatment, increasing plant height, root length, and chlorophyll content at an optimal dose of 0.9 grams per, although shoot growth decreased. These data show that Rhizophora mucronata is more responsive to ammonium- based fertilizer treatments than is Rhizophora stylosa under the evaluated environmental conditions, although the fertilizer dosage had no significant effect. BRIN Publishing 2025-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/8938 10.55981/jbbi.2025.8938 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 14-21 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/8938/8920 Copyright (c) 2025 Fasa Aditya Hanindipto, Fathin Rasima Daulay, Sarwendah Puji Rahayu, Izma Putri Irsani, Alfina Rizki Rachmani, Helga Evangelina https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/8965 2025-01-16T04:07:53Z JBBI:ART

THE EFFECT OF Paederia foetida L. EXTRACT ON LIVER WEIGHT OF MICE SEPSIS MODEL INFECTED WITH Escherichia coli Savitri, Lisa Kasimo, Elfred Rinaldo Krissanjaya, Rochmad Paederia foetida extract Liver weight Mice Sepsis Escherichia coli Sepsis is a critical medical condition characterized by a systemic immune response to in-fection, which can lead to severe organ dysfunction and mortality. Bacterial sepsis, par-ticularly caused by Escherichia coli, poses a significant risk due to its potential to harm tissues and organs, including the liver. The liver plays a central role in metabolic process-es and host defense during sepsis, making it a key organ of interest. This study aimed to investigate the effect of Paederia foetida leaf extract on liver weight in a mice sepsis mod-el. Mice were divided into six groups: normal control (N), negative control (K-) receiving distilled water, positive control (K+) receiving ciprofloxacin, treatment 1 (P1) receiving P. foetida extract at 100 mg/kg BW, treatment 2 (P2) receiving 300 mg/kg BW, and treat-ment 3 (P3) receiving 500 mg/kg BW. After 14 days of treatment, significant differences in liver weight were observed among the groups, with the highest mean and standard de-viation recorded in the P1 group (1.3750 ± 0.3932). Liver abnormalities, including swell-ing, lobular thickening, and weight increase, were identified, indicating the liver’s adap-tive response to toxic substances during sepsis. Interestingly, the normal control group exhibited higher liver weights compared to the treatment groups, possibly due to fatty substance accumulation within the liver tissues. These findings suggest that P. foetida ex-tract may influence liver weight changes in sepsis, potentially modulating metabolic and detoxification processes. Further studies focusing on histopathological and biochemical mechanisms are needed to clarify the therapeutic potential of P. foetida in managing liver dysfunction associated with sepsis. BRIN Publishing 2024-12-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/8965 10.55981/jbbi.2024.8965 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 2 (2024); 388-393 2548-611X 2442-2606 10.55981/jbbi.v11i2 eng https://ejournal.brin.go.id/JBBI/article/view/8965/6973 Copyright (c) 2024 Lisa Savitri, Elfred Rinaldo Kasimo, Rochmad Krissanjaya https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/8978 2025-01-16T04:07:57Z JBBI:ART

THE POTENCY OF BUNIDA F1 AND THEIR CROSSBREED (BUNIDA F2) AS SUPERIOR LOCAL CHICKEN Rahmi, Annisa Baharun, Abdullah Adinda Putri, Tivanka BUNIDA F1 BUNIDA F2 Crossbreed Local chicken Superior genetic High market demands for animal protein have encouraged the optimization of local chicken genetic resources. This research aims to determine the potency of BUNIDA F1 chickens and their crossed-breed (BUNIDA F2). Five BUNIDA F1 hens at 12 months of age are inseminated with Bresse’s semen until they get 50 eggs. The eggs are incubated at 37-38°C and 60-70% humidity. The egg’s fertility and embryo devel-opment were observed on the 4th, 7th, and 18th days of incubation with the candling method. The parameters observed were egg weight, egg fertility, hatchability, and hatching weight. The results show BUNIDA F1 potencies are 54.12 ± 2.70 g egg weight, 82.59 ± 5.11% fertility rate, 71.14 ± 10.67% for hatchability, and hatching weight of BUNIDA F2 is 37.86 ± 1.97 g. It can be concluded that BUNIDA F1 and BUNIDA F2 have the potency to be developed into superior local chickens. BRIN Publishing 2024-12-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/8978 10.55981/jbbi.2024.8978 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 11 No. 2 (2024); 394-399 2548-611X 2442-2606 10.55981/jbbi.v11i2 eng https://ejournal.brin.go.id/JBBI/article/view/8978/6974 Copyright (c) 2024 Annisa Rahmi, Abdullah Baharun, Tivanka Adinda Putri https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/9208 2025-06-11T14:45:15Z JBBI:ART

EFFECTIVENESS OF VARIOUS COMMERCIAL EXTENDERS IN CRYOPRESERVING SEMEN OF LOCAL INDONESIAN RAMS Setiadi, Dedi Rahmat Magfira Riwu, Ronny Markus Jami Arifiantini, R Iis Commercial extenders Frozen semen quality Indonesian local sheep Indonesian local sheep represent a particular species of sheep reared by farmers on small-scale farms. Semen freezing can be performed using either a homemade or commercial extender. The present study evaluated the effectiveness of several commercial extenders in freezing Indonesian local sheep. Semen was collected from three mature and healthy rams using an artificial vagina and evaluated. It was determined that only samples exhibiting sperm motility levels of greater than 70% would be included in the study. Subsequently, the semen from each ram was divided into three portions and diluted with Andromed, Sterydil, and OptiXcell, respectively. The frozen semen was then evaluated 24 hours post-freezing. The thawing process was conducted individually at 37°C for 30 seconds. The evaluation encompassed a range of metrics, including sperm motility, viability, and the presence of any abnormalities, in addition to the recovery rate. The results indicated that sperm frozen in Optixcell and Sterydil extenders exhibited superior motility, viability, and recovery rate than Andromed. The study also demonstrated no interaction between individual Ram and extender, and no differences were found between individuals. The study concluded that Optixcell and Sterydil extenders effectively froze Indonesian local sheep semen. BRIN Publishing 2025-06-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/9208 10.55981/jbbi.2025.9208 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 68-77 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/9208/9003 Copyright (c) 2025 Dedi Rahmat Setiadi, Magfira, Ronny Markus Jami Riwu, R Iis Arifiantini https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/9209 2025-06-11T14:45:15Z JBBI:ART

EXTRACT AND FRACTIONS EXPLORATION OF DAYAK ONION (Eleutherine America-na Merr.) WITH ANTIOXIDANTS POTENTIAL USING GC-MS and FTIR METHODS Elisa, Novi Advistasari, Yustisia Dian Lepangkari, Jaka Seprianto Hidayatullah, Muhammad Haqqi Free Radicals GC-MS FTIR DPPH Free radicals can cause various diseases in the body if not treated immediately. Large levels of free radicals may trigger damage to normally functioning tissues, lipid layers, blood vessels, DNA synthesis disturbances, and even destruction of cells. The objective is to evaluate the antioxidants potential of dayak onion extract and fractions. FTIR spectrophotometers, gas chromatography-mass spectrometry (GC-MS) and an antioxidants multimode reader are some of the research methods. The outcomes of this research are identifying plants that showed the dayak onion extract of the species Eleutherine Americana Merr. It contains compounds such as flavonoids, alkaloids, tannins, and saponins. In addition, the components found in the extract, water fraction, ethyl acetate fraction, and n-hexane fraction are octadecanoic acid 298 m/z, 9-octadecanoic acid 282 m/z, undecanoic acid 214 m/z, hexadecanoic acid 368 m/z, and nonadecanoic acid 326 m/z. Therefore, the antioxidants test using the DPPH method showed that the best IC50 value is for ethyl acetate with IC50 20.56 ppm. BRIN Publishing 2025-06-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/9209 10.55981/jbbi.2025.9209 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 78-84 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/9209/9005 Copyright (c) 2025 Novi Elisa; Yustisia Dian Advistasari, Jaka Seprianto Lepangkari, Muhammad Haqqi Hidayatullah https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/9216 2025-06-11T14:45:14Z JBBI:ART

THE POTENCY OF PSIDIUM GUAJAVA LEAVES AS A NATURAL DISINFECTANT AND ANTISEPTIC INGREDIENT Mahyuni, Siti Komala, Oom Wiendarlina, Ike Yulia Fatah, Muhammad Fajar Saeful Antiseptic Disinfectant Psidium guajava Tannin Preventing the spread of nosocomial infection are generally carried out by disinfection process of the tools to be used with chemical disinfectant such as phenol, alcohol, chlorine, iodine or sublimate. Excessive use of chemicals can cause health problems. Guava leaves (Psidium guajava L.) have long been empirically used to treat diarrhea and acne. Scientific data shows that guava leaves have positive activity against several types of pathogenic bacteria due to its tannin content. Guava leaves have the potential to be developed as a disinfectant and antiseptic material. This study aims to determine the disinfection activity of guava leaves on surgical instruments using the total plate count method, skin irritation test using patch test method and quantify the tannin content in young and old guava leaves using spectrophotometry method. The results showed that the tannin content in old and young guava leaves was 68.73 ± 0.067 mg/g (6.873%) and young 61.87 ± 0.046 mg/g (6.187%). At concentrations of 10, 20, 30 and 40% guava leaf extract were able to reduce the total plate count in surgical instrument samples by 6.85; 37; 80.82; and 83.56%. The primary irritation index of 0.05 only occurs at a concentration of 40%, classified as non-irritant. It can be concluded that guava leaves have the potential to be further developed as a natural disinfectant or antiseptic. BRIN Publishing 2025-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/9216 10.55981/jbbi.2025.9216 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 22-30 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/9216/8921 Copyright (c) 2025 Siti Mahyuni, Oom Komala, Ike Yulia Wiendarlina, Muhammad Fajar Saeful Fatah https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/9246 2025-06-11T14:45:15Z JBBI:ART

OPTIMIZING VIRGIN COCONUT OIL YIELD AND QUALITY USING LACTIC ACID BACTERIA FROM BLONDO WITH CHILLING-THAWING METHOD Chaidir, Riri Rimbun Anggih Jariah, Ainun Nevara, Gita Addelia Murti, Tri Rohayu, Andini Cindy BAL Chilling-thawing Lactic Acid Bacteria Oil extraction VCO Increasing demand for Virgin Coconut Oil (VCO) presents a promising opportunity to improve the economy in Indonesia. Various methods for VCO production have been explored to optimize yield and ensure desirable organoleptic and physicochemical qualities. Modifying the oil extraction process often necessitates costly equipment. In this study, VCO was extracted by combining the chilling-thawing method with the addition of lactic acid bacteria (LAB) isolated from blondo. The process began with the isolation and characterization of LAB isolates, followed by the application of the chilling-thawing method and addition of 2% LAB starter, then assessed for their consumer preference through a questionnaire. This innovative process yielded VCO at a rate of 35.78-46.65% using three LAB isolates (BAL 1-3) from blondo samples, representing an improvement over previous studies employing similar methodologies. Moreover, hedonic evaluation revealed a higher consumer preference for LAB-treated VCO compared to the control, further highlighting the effectiveness of LAB treatment in combination with the chilling-thawing method. BRIN Publishing 2025-06-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/9246 10.55981/jbbi.2025.9246 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 55-67 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/9246/8998 Copyright (c) 2025 Riri Rimbun Anggih Chaidir, Ainun Jariah, Gita Addelia Nevara, Tri Murti, Andini Cindy Rohayu https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/9257 2025-06-11T14:45:15Z JBBI:ART

EFFECT OF GROWING MEDIA ON THE GROWTH OF Streptomyces sp. AND Trichoderma harzianum AS ANTHRACNOSE DISEASE BIOCONTROL Rulinggar, Nia Mujoko, Tri Wuryandari, Yenny Streptomyces sp. Trichoderma harzianum Growing media Colletototrichum capsici Biocontrol Streptomyces sp. and Trichoderma harzianum is a biological agent that is effective in controlling Colletotrichum capsici, the cause of disease in chili plants. Different growing media can affect the growth and activity of these two microorganisms. This study aims to evaluate the growth of Streptomyces sp. and Trichoderma harzianum on various media, namely rice washing water, coconut water, and sugar potato extract. The research phase includes growing media test, effectiveness test of Streptomyces sp. and Trichoderma harzianum in chili peppers, and effectiveness test of Streptomyces sp. and Trichoderma harzianum on chili seeds. The results showed that the medium of rice washing water is more suitable for Streptomyces sp., coconut water media is more suitable for Trichoderma harzianum, and rice washing water media is more suitable for the combination of the two biological agents. Optimal growth in these media affect the effectiveness of biocontrol in suppressing the severity of anthracnose disease in chili. BRIN Publishing 2025-06-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/9257 10.55981/jbbi.2025.9257 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 130-143 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/9257/9014 Copyright (c) 2025 Nia Rulinggar, Tri Mujoko, Yenny Wuryandari https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/9338 2026-01-28T04:40:27Z JBBI:ART

RECENT UPDATE OF ZERUMBONE AS ANTI-COLON CANCER AGENT: A REVIEW Sarmoko, Sarmoko Fauziyya, Riri Zahra, Miralda Suprahman, Nisa Yulianti Auli, Winni Nur Ashari, Arif apoptosis chemoresistance colon cancer inflammation zerumbone Colorectal cancer (CRC) remains a leading cause of cancer-related deaths worldwide, with limited effective therapies due to drug resistance and adverse effects. Zerumbone, a sesquiterpene isolated from Zingiber zerumbet Smith, has emerged as a promising natural anticancer agent. This review examines zerumbone's anti-colorectal cancer properties, including induction of apoptosis and cell cycle arrest, inhibition of invasion and metastasis, anti-angiogenic activity, and anti-inflammatory effects. Additionally, zerumbone demonstrates antioxidant properties, modulates gut microbiota composition, and targets multiple signaling pathways involved in CRC pathogenesis. Structure-activity relationship studies reveal the critical role of the α,β-unsaturated carbonyl group in its bioactivity. Despite promising preclinical evidence, clinical validation remains necessary to establish zerumbone's therapeutic potential for colorectal cancer management. BRIN Publishing 2025-12-24 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/9338 10.55981/jbbi.2025.9338 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 2 (2025); 350-360 2548-611X 2442-2606 10.55981/jbbi.v12i2 eng https://ejournal.brin.go.id/JBBI/article/view/9338/11291 Copyright (c) 2025 Sarmoko Sarmoko, Riri Fauziyya, Miralda Zahra, Nisa Yulianti Suprahman, Winni Nur Auli, Arif Ashari https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/9390 2025-06-11T14:45:15Z JBBI:ART

PHARMACOLOGICAL INVESTIGATION OF THE ORAL WOUND HEALING ACTIVITY OF Stachytarpheta jamaicensis ROOT EXTRACT GEL IN WISTAR RATS Utami, Juliyatin Putri Wasiaturrahmah, Yusrinie Carabelly, Amy Nindia Aspriyanto, Didit Nahzi, Yanuar Ichrom Arifin, Rahmad Ramadhaniyah, Nur Jatmiko, Habibi Naufal Epithelialization Hydroxyproline. Inflammation Cells Stachytarpheta jamaicensis Wound healing The Stachytarpheta jamaicensis (L) Vahl root contains bioactive compounds like saponins, alkaloids, flavonoids, tannins, phenols, and terpenoids, which have antibacterial and anti-inflammatory properties. This study investigates the wound healing effects of Stachytarpheta jamaicensis (L) Vahl root extract gel in oral mucosa. The experiment involved 28 male Wistar rats, divided into four groups: a positive control treated with povidone iodine 10%, a negative control treated with gel base, and two experimental groups treated with 6% and 10% S. jamaicensis root extract gels. The gel was applied twice daily, and wound healing was assessed by epithelial thickness, inflammation cell and hydroxyproline content. Both concentrations of S. jamaicensis root extract gel significantly enhanced wound healing, as shown by increased epithelial thickness, inflammation cell and hydroxyproline content levels compared to the negative control. These findings suggest that S. jamaicensis root extract gel effectively promotes wound healing in the excision wound model. BRIN Publishing 2025-06-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/9390 10.55981/jbbi.2025.9390 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 85-95 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/9390/9006 Copyright (c) 2025 Juliyatin Putri Utami, Yusrinie Wasiaturrahmah, Amy Nindia Carabelly, Didit Aspriyanto, Yanuar Ichrom Nahzi, Rahmad Arifin, Nur Ramadhaniyah, Habibi Naufal Jatmiko https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/9769 2025-06-11T14:45:14Z JBBI:ART

THE COMPARISON BETWEEN LOGISTIC REGRESSION AND CONVOLUTIONAL NEURAL NETWORK FOR MULTI-DRUG RESISTANT TUBERCULOSIS PREDICTION Widjaja, Albert Wibowo, Satrio Parikesit, Arli Aditya Convolutional Neural Network Machine Learning MDR-TB Mycobacterium tuberculosis Logistic Regression Multi-drug resistant tuberculosis (MDR-TB) is caused by Mycobacterium tuberculosis strains that resist at least two first-line anti-TB drugs. This disease presents a major global health challenge, particularly affecting middle to lower income countries where affordable and rapid diagnostic tools are urgently needed. To address this, researchers are exploring the combination of whole genome sequencing and machine learning for drug resistance predictions. Using Mycobacterium tuberculosis genomic data from databases, both Logistic Regression (LR) and Convolutional Neural Network (CNN) models were trained to predict drug resistance. Performance evaluation revealed that CNN slightly outperformed LR in accuracy and specificity for Rifampicin and Pyrazinamide predictions, while LR showed better results for Isoniazid and Ethambutol. In terms of sensitivity, LR demonstrated superior performance for most drugs, except Ethambutol where CNN excelled. Though computational complexity assessment was incomplete due to hardware limitations, both models showed distinct advantages in predicting first-line anti-TB drug resistance. BRIN Publishing 2025-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/9769 10.55981/jbbi.2025.9769 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 31-44 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/9769/8922 Copyright (c) 2025 Albert Widjaja, Satrio Wibowo, Arli Aditya Parikesit https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/10087 2025-06-11T14:45:14Z JBBI:ART

FROZEN SEMEN CHARACTERISTICS OF BALI BULL IN DIFFERENT AGE GROUPS Witri, Brilla Widya Arifiantini, Iis Prasetyaningtyas, Wahono Esthi Karja, Ni Wayan Kurniani Age Bali bulls Frozen semen Frozen semen characteristics One factor that affects frozen semen characteristics is bull age. Increasing age induces changes in sperm that reduce frozen semen quality. This study aimed to evaluate the frozen semen characteristics of Bali bulls aged 3, 8, and 13 years. Frozen semen was derived from fresh semen with sperm motility > 70%. The frozen semen characteristics tested included motility, viability, plasma membrane integrity, acrosome integrity, and sperm abnormalities. Sperm motility was analyzed by computer-assisted sperm analysis (CASA). Viability was assessed using eosin-nigrosin staining; plasma membrane integrity was assessed using the hypoosmotic swelling test (HOST); acrosome integrity was assessed using Giemsa staining; and sperm abnormalities were assessed using Williams staining. The plasma membrane integrity of the 13-year-old group was significantly lower (P < 0.05) than that of the younger age groups. However, there were no significant differences (P > 0.05) in the other parameters. We conclude that age affects the integrity of the sperm plasma membrane in frozen semen from Bali bulls. BRIN Publishing 2025-06-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/10087 10.55981/jbbi.2025.10087 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 45-54 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/10087/8997 Copyright (c) 2025 Brilla Widya Witri, Iis Arifiantini, Wahono Esthi Prasetyaningtyas, Ni Wayan Kurniani Karja https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/10238 2025-06-11T14:45:15Z JBBI:ART

THE DIVERSITY AND CHARACTERISATION OF CELLULOLYTIC BACTERIA ISOLATED FROM VARIOUS CONDITIONS OF EMPTY FRUIT BUNCHES IN OIL PALM PLANTATION Agam Pamungkas, Edo Madusari, Sylvia Putri, Halida Adistya Rosita, Risa molecular identification DNA barcoding Cellulolytic Bacteria Oil palm empty fruit bunches Cellulase Enzyme The utilisation of oil palm empty fruit bunches (EFB) remains underexplored. Harnessing cellulolytic microorganisms for the production of cellulase enzymes offers sustainable approach to addressing waste management challenges while aligning with the principles of the Sustainable Development Goals (SDGs) and address waste management challenges. This study aims to isolate, characterize, identify, and test the potential cellulase activity of cellulolytic bacteria from EFB taken from three different locations: PO code from organic fertilizer plantations (POU1, POU2, POU3), PL code from oil palm plantations (PLU1, PLU2, PLU3), and PK code from Sulung mills (PKU1, PKU2, PKU3). This study used three isolated cultures in its testing. The research process includes sample preparation, bacterial isolation, gram staining, catalase test, hypersensitivity test, DNA amplification, bioinformatics analysis and cellulase activity analysis. The results of the bacterial isolation obtained 28 colonies. The results of the characterisation were all 3 non-pathogenic bacterial isolates, with a positive catalase test. The result of staining Gram-negative with bacilli-shaped bacteria. The amplification results obtained a band size of 1500 bp. The results of the identification obtained the species Aeromonas enteropelogenes, Nitrosomonas stercoris, and Methylobacillus caricis. The results of phylogenetic analysis showed low homology. Cellulase activity of six positive isolates with medium ability isolates code POU3 (1.3), PLU2 (1.0), PLU3 (1.0); low isolates POU1 (0.2), POU2 (0.2), PLU (0.8) and 3 negative isolates no enzyme activity PKU1 (-1), PKU2 (-1), and PKU3 (-1). BRIN Publishing 2025-06-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/10238 10.55981/jbbi.2025.10238 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 119-129 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/10238/9012 Copyright (c) 2025 Edo Agam Pamungkas, Sylvia Madusari, Halida Adistya Putri, Risa Rosita https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/10400 2025-12-28T08:47:07Z JBBI:ART

COMPARATIVE ASSESSMENT OF BULL SPERM MOTILITY AND CONCENTRATION: CONVENTIONAL METHODS VERSUS PORTABLE ANDROSCOPE CASA SYSTEM Arifiantini, Raden Iis Gusman, Khalis Talitha Agil, Muhammad Karja, Ni Wayan Kurniani AndroScope CASA Semen Analysis Sperm Motility Sperm Concentration Operational implementation, such as laboratory staff and equipment, differ for each frozen semen producer, resulting in variability in the quality assessment of the frozen semen produced. Conventional assessments subject to subjectivity, human error, and high variability. Computer-assisted semen analysis (CASA) is considered more objective. This study aimed was to conduct a comparative analysis of motility and concentration assessment of frozen semen sperm using conventional and the portable CASA AndroScope. Twenty-one laboratory assistants from 21 national and regional frozen semen producers participated in this study. Sperm motility and concentration were assessed conventionally (using a Neubauer chamber) and AndroScope was used with five replicates. The results of the comparison of sperm motility scores revealed significant differences (p<0.05) between conventional examination and using AndroScope. The results of the comparison of the sperm concentration calculations revealed no significant difference (p>0.05) between the calculations performed using the Neubauer chamber and AndroScope. Assessment with the AndroScope is considered reliable and can replace conventional assessment in assessing motility and sperm concentration, thereby improving and standardizing the assessment performed by frozen semen producers. BRIN Publishing 2025-12-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/10400 10.55981/jbbi.2025.10400 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 2 (2025); 286-292 2548-611X 2442-2606 10.55981/jbbi.v12i2 eng https://ejournal.brin.go.id/JBBI/article/view/10400/11109 Copyright (c) 2025 Raden Iis Arifiantini, Khalis Talitha Gusman, Muhammad Agil, Ni Wayan Kurniani Karja https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/10407 2025-12-28T08:47:07Z JBBI:ART

CHARACTERISTICS AND KINEMATICS OF RAM FROZEN-THAWED SPERM MOVEMENT WITH DIFFERENT LECITHIN TYPES Damanik, Asman Ramadhan Karja, Ni Wayan Kurniani Setiadi , Mohamad Agus Kaiin, Ekayanti Mulyawati Computer Assisted Sperm Analysis Kinematics Lecithin Sperm This study evaluated the effectiveness of animal, plant, and synthetic lecithin-based extenders in preserving local Indonesian ram semen during cryopreservation. Post-thaw sperm quality was assessed using Computer-Assisted Sperm Analysis (CASA), focusing on motility, viability, membrane integrity, and kinematics. Data were analyzed using ANOVA and Duncan’s Multiple Range Test (DMRT) at a 95% significance level. Results showed that sperm motility, viability, and membrane integrity were significantly higher (P<0.05) in the animal lecithin-based extender (53.66±1.33%, 66.45±2.50%, and 67.68±2.33%, respectively) compared to plant (51.84±0.48%, 62.98±1.94%, and 64.27±1.51%) and synthetic (50.14±0.22%, 60.72±0.81%, 62.44±1.37%) extenders. While kinematic parameters were not significantly different among groups, synthetic lecithin showed slightly higher values in velocity-related measures, whereas animal lecithin resulted in the most consistent improvement in overall sperm quality. All lecithin-based extenders met the Indonesian National Standard (SNI) for semen quality, and sperm kinematics remained within optimal fertilization values after cryopreservation. BRIN Publishing 2025-12-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/10407 10.55981/jbbi.2025.10407 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 2 (2025); 293-301 2548-611X 2442-2606 10.55981/jbbi.v12i2 eng https://ejournal.brin.go.id/JBBI/article/view/10407/11110 Copyright (c) 2025 Asman Ramadhan Damanik, Ni Wayan Kurniani Karja, Mohamad Agus Setiadi , Ekayanti Mulyawati Kaiin https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/10477 2025-06-18T14:11:36Z JBBI:ART

IDENTIFICATION OF KEY NITROGEN USE EFFICIENCY-RELATED GENES IN OIL PALM USING BIOINFORMATICS APPROACHES Permatasari, Galuh Wening Setiowati, Retno Diah Wening, Sri Nitrogen use efficiency Protein-protein interaction structural modelling CESA genes Efficient nitrogen use is crucial for maximizing oil palm yield while reducing environmental impact. Poor nitrogen utilization causes excessive growth and nutrient loss. This study uses bioinformatics to identify key genes linked to nitrogen use efficiency, providing insights for genetic improvement and sustainable cultivation.Protein-protein interaction (PPI) networks, functional enrichment, and structural modeling were employed to uncover candidate genes regulating nitrogen uptake and metabolism. Sixty-two nitrogen use efficiency associated genes from rice (Oryza sativa) were analyzed via BLASTp against the E. guineensis genome (NCBI), selecting those with >80% similarity. PPI networks were constructed using STRING-db and analyzed in Cytoscape v3.7.1. Functional enrichment (Gene Ontology) and structural analysis (AlphaFold, PyMol v2.5.4) were performed. Twelve nitrogen use efficiency related genes were identified, with CESA4, CESA7, and CESA9 emerging as key regulators based on high degree and betweenness values in PPI analysis. These genes are linked to plant cell wall biosynthesis. Structural analysis showed high similarity to rice homologs, with RMSD values of 0.338 Å (CESA4) and 0.396 Å (CESA9), indicating strong conservation area. Their structural relevance suggests they are promising targets for molecular breeding marker to enhance nitrogen utilization and sustainability in oil palm. BRIN Publishing 2025-06-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/10477 10.55981/jbbi.2025.10477 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 194-211 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/10477/9298 Copyright (c) 2025 Galuh Wening Permatasari, Retno Diah Setiowati, Sri Wening https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/10646 2026-01-28T04:47:27Z JBBI:ART

OPTIMIZATION AND EFFICIENCY ANALYSIS OF REAL-TIME PCR FOR LEPTOSPIRA SPP. DIAGNOSIS BASED ON THE LIPL32 GENE Sulaeman, Rizal Pratama Rohayati Merdekawati, Fusvita Sundara Mulia, Yuliansyah Leptospirosis LipL32 gene Molecular Diagnosis PCR efficiency Real-time PCR Leptospirosis is a widespread zoonotic infection that endangers the health of both humans and animals, particularly in tropical and subtropical regions. Therefore, timely, sensitive and specific laboratory confirmation is essential for early clinical management. The LipL32 gene is a highly conserved virulence factor in pathogenic Leptospira. Real-time PCR provides rapid detection with high sensitivity and specificity. This study optimized real-time PCR conditions by evaluating annealing temperatures (60°C, 61°C, 62°C), primer concentrations (500 nM, 700 nM, 900 nM), and probe concentrations (250 nM, 300 nM). PCR efficiency was analyzed using absolute quantification with serial DNA dilutions (100 to 10⁻⁴). The optimal conditions were 60°C annealing temperature, 500 nM primers, and 300 nM probes. Near-ideal efficiency (97%) was achieved at 60°C with 500 nM primers and 250 nM or 300 nM probes, while 103% efficiency was observed at 61°C with 500 nM primers and 250 nM probes. This optimization enhances Leptospira detection accuracy using real-time PCR. BRIN Publishing 2025-12-24 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/10646 10.55981/jbbi.2025.10646 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 2 (2025); 382-390 2548-611X 2442-2606 10.55981/jbbi.v12i2 eng https://ejournal.brin.go.id/JBBI/article/view/10646/11294 Copyright (c) 2025 Rizal Pratama Sulaeman, Rohayati, Fusvita Merdekawati, Yuliansyah Sundara Mulia https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/10708 2025-06-11T14:45:15Z JBBI:ART

BLOOD SMEAR EXAMINATION AND DIFFERENTIAL COUNT IN RATS WITH ESCHERICHIA COLI ESBL AND KLEBSIELLA PNEUMONIAE CARBAPENEMASE INFECTIONS Savitri, Lisa Kasimo, Elfred Rinaldo Ihsan, Kharisul Faizal, Imam Agus differential count sepsis Escherichia coli ESBL Klebsiella pneumoniae carbapenemase Sepsis is an irregular body response to severe infection, triggering uncontrolled inflammation that can lead to extensive tissue damage. It can progress to septic shock with multiple organ failure, resulting in death if left untreated. Laboratory examinations, such as leukocyte differential count in hematology, help understand the distribution pattern of white blood cells associated with health conditions. Laboratory research was conducted on mice injected with E. coli ESBL or K. pneumoniae carbapenemase. After 24 hours, observations were made on apoptosis in the spleen and liver of mice. Mouse blood was processed to count white blood cell types with a differential count. The results were analyzed to compare the control group with the bacterial infection groups of E. coli ESBL and K. pneumoniae carbapenemase. The research results indicate that the neutrophil count in the E. coli ESBL group is still within the normal range and lower (44.5±1.915%) compared to the K. pneumoniae carbapenemase group (55.75±8.342%). Similarly, the lymphocyte count in the E. coli ESBL group is within the normal range and lower (77.5±3.109%) compared to the K. pneumoniae carbapenemase group (91.25±7.588%). This highlights the crucial role of neutrophils and lymphocytes in responding to severe bacterial infections such as K. pneumoniae carbapenemase. Previous studies indicate neutrophilia and lymphocytopenia as markers of severe bacterial infections. Neutrophils are the primary defense against bacterial infections and can be rapidly recruited to the infection site, while specific infections can trigger prolonged neutrophil recruitment from hematopoietic tissues. BRIN Publishing 2025-06-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/10708 10.55981/jbbi.2025.10708 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 96-103 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/10708/9007 Copyright (c) 2025 Lisa Savitri, Elfred Rinaldo Kasimo, Kharisul Ihsan, Imam Agus Faizal https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/10931 2025-06-11T14:45:15Z JBBI:ART

DECOLORIZATION OF TEXTILE DYES WITH CRUDE LIGNINOLYTIC ENZYMES FROM Coriolopsis caperata Agnestisia, Retno Karelius, Karelius Oksal, Efriyana Nion, Yanetri Asi Coriolopsis caperata decolorization ligninolytic enzymes textile dye The textile industry is a major contributor to global water pollution, releasing an estimated 280,000 tons of synthetic dyes annually into aquatic ecosystems. These dyes, particularly azo compounds, are often toxic, mutagenic, and resistant to conventional wastewater treatment methods. Microbial enzymatic degradation, especially by white-rot fungi, offers a promising eco-friendly alternative. This study aimed to optimize the production of ligninolytic enzymes—Laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP)—from Coriolopsis caperata, and assess their efficiency in degrading two azo dyes: Reactive Red 21 (RR21) and Reactive Orange 107 (RO107). The fungus, isolated from the Peat Swamp Forest in Sebangau, Central Kalimantan, was cultured in a modified glucose-peptone medium enriched with veratryl alcohol. The optimization parameters included variations in time, dye concentration, and the addition of hydrogen peroxide (H₂O₂). Enzyme activity was quantified spectrophotometrically, and dye decolorization was assessed over time at different dye concentrations. Among the enzymes, Lac showed the highest activity (4938.05 U/L), followed by LiP (995.26 U/L) and MnP (246.47 U/L). These values notably exceed several previously reported benchmarks for fungal enzyme activity. RO107 demonstrated greater susceptibility to enzymatic degradation, with 83.71% decolorization achieved at 24 hours, while RR21 reached 65.71% at 48 hours. The addition of 1 mM H₂O₂ significantly enhanced decolorization, increasing RR21 and RO107 removal to 95.71% and 99.30%, respectively. These results underscore the oxidative synergy between H₂O₂ and ligninolytic enzymes, particularly LiP and MnP. Overall, the study demonstrates the potential scalability of C. caperata-based enzymatic treatment systems for textile effluent bioremediation, supporting compliance with environmental discharge regulations and contributing to sustainable wastewater management. BRIN Publishing 2025-06-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/10931 10.55981/jbbi.2025.10931 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 104-118 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/10931/9010 Copyright (c) 2025 Retno Agnestisia, Karelius Karelius, Efriyana Oksal, Yanetri Asi Nion https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/11244 2025-07-17T14:18:12Z JBBI:ART

THE INFLUENCE OF PHYSICOCHEMICAL PARAMETERS ON THE ABUNDANCE OF MACROZOOBENTHOS IN KUNGKILAN RIVER, SOUTH SUMATERA Teristiandi, Novin Yuliana, Meta Bioindicators Spatial Variation Tropical Rivers Functional Feeding Groups Bioassessment Macrozoobenthos are effective bioindicators for assessing freshwater ecosystem health. This study examines the influence of physicochemical parameters and spatial-temporal variation on macrozoobenthic abundance in the Kungkilan River, South Sumatera. Sampling was conducted at upstream, midstream, and downstream sites. Generalized Linear Mixed Model (GLMM) analysis revealed that water depth and transparency had significant positive effects, while iron (Fe) in sediment had a negative effect. Random effects analysis showed that site-level variability (variance = 0.680) was far greater than temporal variability, indicating that spatial differences—especially in the midstream zone—were the dominant drivers of community structure. These findings highlight the importance of localized conservation efforts and reinforce the role of macrozoobenthos as reliable indicators of river health in tropical ecosystems. BRIN Publishing 2025-07-17 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/11244 10.55981/jbbi.2025.11244 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 228-236 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/11244/9463 Copyright (c) 2025 Novin Teristiandi, Meta Yuliana https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/11318 2025-06-11T14:45:15Z JBBI:ART

IDENTIFICATION AND CHARACTERIZATION OF MICROPLASTIC DEGRADING BACTERIA IN THREE LANDFILLS OF LAMPUNG PROVINCE Hening, Jeni Latri Kamelia, Marlina Kesuma, Andri Jaya Bacteria Biodegradation Characterization Landfill Microplastic Microplastics are durable and contain harmful compounds that can be absorbed into the soil and enter the food chain, posing a risk to human health and the environment. One of the efforts to reduce its impact is to utilize bacteria as biodegradation agents. The purpose of this study was to isolate and characterize microplastic degrading bacteria from three landfills in Lampung Province, and determine their degradation rate. The research method used a quantitative descriptive approach, including the isolation stage with the pouring technique, degradation tests using Polystyrene, Polyethylene Terepthalate, and Polyethylene measuring 1x1 cm and identification using Vitek-2. The results of the study from nine bacterial isolates that were successfully purified, three of them showed the highest ability to degrade. The weight reduction result on PS plastic sample was 22.2% by Bacillus circulans, PET sample was 15.9% by Pandoraea spp. and PE sample was 36.2% by Pseudomonas aeruginosa bacteria. BRIN Publishing 2025-06-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/11318 10.55981/jbbi.2025.11318 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 144-156 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/11318/9015 Copyright (c) 2025 Jeni Latri Hening, Marlina Kamelia, Andri Jaya Kesuma https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/11320 2025-06-18T14:11:36Z JBBI:ART

THE EFFECT OF POST-PRODUCTION SHELF TIME ON THE ANTIOXIDANT ACTIVITY OF SOY MILK Fadhillah Nazip, Khoiron Meilinda Antioxidants DPPH Post Production Save Time Soy Milk This study was conducted to analyze the effect of post-production storage time on antioxidant activity in soy milk. Antioxidants are compounds that can ward off free radicals, so it is important to know their stability over time. The storage time used in this study was 1, 4, 7, 10, and 13 hours post-production. Antioxidant activity testing was carried out using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Qualitatively, the color change of the DPPH solution was observed from purple to light yellow, while quantitatively the absorbance value was measured using UV-Vis Spectrophotometry. The inhibition value and IC50 were calculated to determine the strength of antioxidant activity in soy milk. The results showed that soy milk with a storage time of 1 hour had the best antioxidant activity with an IC50 value of 28.19 μg/ml which was classified as very strong. At 4 hours of storage time, the IC50 value of 37.28 μg/ml is classified as moderate, while at 7 hours and 10 hours the IC50 values ​​were 146.95 μg/ml and 119.38 μg/ml, respectively, which are also classified as moderate. At 13 hours, the antioxidant activity decreased drastically with an IC50 value of 228.90 μg/ml, which is classified as very weak. The antioxidant activity of soy milk decreased with increasing storage time. Factors such as temperature, light, and components of soy milk contribute to the degradation of antioxidant activity. Therefore, consumption of soy milk should be done within a shorter time after production to obtain optimal antioxidant benefits. BRIN Publishing 2025-06-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/11320 10.55981/jbbi.2025.11320 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 171-181 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/11320/9052 Copyright (c) 2025 Fadhillah, Khoiron Nazip, Meilinda https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/11333 2026-01-28T04:49:11Z JBBI:ART

VIOLACEIN: IT'S ANTICANCER PROPERTIES Santoso, Adit Widodo Simamora, Adelina Margretha, Margretha Timotius, Kris Herawan apoptosis autophagy bacterial secondary product microenvironment signalling pathway Violacein is a bacterial secondary product with various bioactivities, including anticancer activities. This narrative review aimed to evaluate anticancer potentials based on its modes of action, either at cellular, subcellular, or molecular levels or in tumour microenvironment. At cellular level, violacein can inhibit cancer cell proliferation, arrest cell cycle, induce apoptosis, autophagy, and cell differentiation. At subcellular level, violacein can modulate processes in mitochondria. At molecular level, violacein can generate reactive oxygen species, attenuate inflammation, repair oncogenes, upregulate suppression genes, inhibit or activate several cancer vital enzymes, and control various signalling pathways. Violacein indirectly influences communication between cancer cells and their tumour microenvironment by inducing apoptosis and autophagy and inhibiting metalloproteinases and angiogenesis. Violacein inactivates several signalling pathways, including MAPK, Akt/NF-kB, JAK2/STAT3, and TGFβ, which are essential for cancer cell development. Violacein is a promising anticancer drug candidate with broad coverage of various cancer diseases and diverse modes of action. BRIN Publishing 2025-12-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/11333 10.55981/jbbi.2025.11333 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 2 (2025); 426-449 2548-611X 2442-2606 10.55981/jbbi.v12i2 eng https://ejournal.brin.go.id/JBBI/article/view/11333/11316 Copyright (c) 2025 Adit Widodo Santoso, Adelina Simamora, Margretha Margretha, Kris Herawan Timotius https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/11335 2026-05-11T02:26:43Z JBBI:RVW

EFFECTS OF ZINC SUPPLEMENTATION ON REPRODUCTIVE PERFORMANCE IN CATTLE: A REVIEW Akil, Syahrir Damayanti, Erni Samsudewa, Daud Hapila, Ashariah Iskandar, Hikmayani Dosage Reproductive Performance Bali cattle Zinc Zinc is a vital micronutrient involved in various reproductive processes in cattle. This review aims to evaluate the effects of zinc supplementation on cattle reproductive performance by analyzing research articles sourced from Scopus and PubMed databases. Findings indicate that zinc supplementation, provided in either organic or inorganic forms, positively influences reproductive parameters. In bulls, zinc enhances sperm motility, viability, and ejaculate volume. In female cattle, supplementation modulates endocrine hormone levels during both pre-calving and post-calving periods. Moreover, appropriate zinc dosages and supplementation durations contribute to improved reproductive efficiency in cows. These results support the conclusion that zinc supplementation is effective in enhancing reproductive performance in cattle. BRIN Publishing 2025-06-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://ejournal.brin.go.id/JBBI/article/view/11335 10.55981/jbbi.2025.11335 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 163-170 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/11335/9051 Copyright (c) 2025 Syahrir Akil, Erni Damayanti, Daud Samsudewa, Hikmayani Iskandar https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/11346 2025-06-18T14:11:36Z JBBI:ART

VITAMIN C SUPPLEMENTATION IN EGG YOLK CITRATE EXTENDER OPTIMIZES BANGKOK ROOSTER SEMEN QUALITY DURING COLD STORAGE Ananda Faizal, Noval Jumatul Jaswandi Bangkok rooster extender liquid semen sperm quality vitamin C This study aimed to evaluate the effect of different vitamin C concentrations (0, 0.1, 0.2, and 0.3 g/100 mL) in egg yolk citrate extender and storage time (0, 24, 48, 72, and 96 hours) on the quality of Bangkok rooster semen stored at 5°C. Using a split-plot-in-time design with four replications, semen quality was assessed for motility, viability, abnormality, intact plasma membrane (IPM), and longevity. Results showed a significant interaction (P<0.01) between vitamin C concentration and storage time on motility, and significant effects (P<0.05) on viability, abnormality, and IPM. The 0.2 g vitamin C dose preserved >40% motility and >50% viability for 96 hours and extended sperm longevity up to 11.25±0.95 days. These findings suggest that 0.2 g/100 mL vitamin C is the optimal concentration for preserving rooster semen quality during short-term cold storage. BRIN Publishing 2025-06-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/11346 10.55981/jbbi.2025.11346 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 182-193 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/11346/9053 Copyright (c) 2025 Ananda, Noval Jumatul Faizal, Jaswandi https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/11402 2025-12-22T06:48:43Z JBBI:ART

ANALYSIS OF THE RATIONALITY OF ANTIPLATELET USE IN ISCHEMIC STROKE PATIENTS AT “X” HOSPITAL, SEMARANG CITY Wibowo, Sulistiyanto Dian A, Yustisia Hasanah A.M, Restu Antiplatelet Evaluation Ischemic Stroke Rational Drug Use Semarang City Ischemic stroke accounts for approximately 85% of all stroke cases and remains a major global health problem. Antiplatelet agents are key for secondary prevention, but irrational use may reduce effectiveness and increase bleeding risk. This retrospective descriptive study evaluated the rationality of antiplatelet use in ischemic stroke patients at “X” Hospital, Semarang City, from January to July 2024. Patients aged ≥26 years with complete medical records were included. Rationality was assessed based on PNPK Stroke Guidelines (2019), AHA/ASA Guidelines (2021), and AHFS Drug Information (2024) using three indicators: appropriate drug, dose, and patient. A total of 111 patients (113 cases) met the inclusion criteria. Most were male (58.56%) and aged >65 years (36.04%), with hypertension, diabetes, and hyperlipidemia as common comorbidities. Aspirin was the most used monotherapy (38.05%), and aspirin–clopidogrel the most frequent combination (37.17%). Drug selection accuracy was high (96.46%), while dose accuracy was low (44.25%). Antiplatelet use at “X” Hospital demonstrated rational drug selection but suboptimal dosing. Improved adherence to dosing guidelines and periodic prescription review are needed to enhance patient safety and treatment outcomes. BRIN Publishing 2025-12-13 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/11402 10.55981/jbbi.2025.11402 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 2 (2025); 171-176 2548-611X 2442-2606 10.55981/jbbi.v12i2 eng https://ejournal.brin.go.id/JBBI/article/view/11402/11085 Copyright (c) 2025 Sulistiyanto Wibowo, Yustisia Dian A, Restu Hasanah A.M https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/11410 2025-06-18T01:26:55Z JBBI:ART

KINEMATICS OF SPERMATOZOA IN FRESH AND FROZEN SEMEN OF PESISIR CATTLE Ramadhan, Rio Ananda Hendri CASA Evaluation Fresh semen Frozen semen Pesisir cattle This study aimed to evaluate the quality and kinematics of spermatozoa in fresh and frozen semen of coastal cattle. Semen of coastal cattle was collected using an artificial vagina from 2 males, fresh semen was tested and frozen using tris egg yolk diluent. Fresh and frozen semen of coastal cattle were evaluated using a Computer Assisted Sperm Analyzer (CASA). This study used a t-test analysis by comparing the quality and kinematics of spermatozoa of fresh semen and frozen semen of coastal cattle. The parameters observed were macroscopic, microscopic and CASA quality observations. The results showed the kinematics of fresh and frozen semen motility (83.83%; 71.68%) and the kinematics of progressive motility of fresh and frozen semen (80.81%; 59.56%). Based on the results of the t-test analysis, it was found that t count > t table, the kinematics of fresh semen spermatozoa had a significant effect on the frozen semen of Pesisir cattle on the motility of fresh semen and frozen semen, on the progressive motility of fresh semen and frozen semen. The characteristic values ​​of kinematics in fresh semen and frozen semen of Pesisir cattle were respectively velocity curvilinear (VCL) 178.94 μm/s and 108.52 μm/s; velocity straight line (VSL) 96.61 μm/s and 49.34 μm/s; velocity average path (VAP) 114.99 μm/s and 64.04 μm/s; linearity (LIN) 0.53% and 0.44%; straightness (STR) 0.83% and 0.75%; wobble (WOB) 0.64% and 0.58%; lateral head displacement (ALH) amplitude 5.83 μm and 5.15 μm; beat cross frequency (BCF) 35.28 Hz and 24.65 Hz. The kinematics of fresh and frozen semen spermatozoa significantly affect the value of motility characteristics. The conclusion of the results of this study is that there is a decrease in the quality of fresh semen kinematics and frozen semen kinematics spermatozoa on the value of motility characteristics, namely on the speed of spermatozoa at the velocity curvilinear (VCL), velocity straight line (VSL), and velocity average path (VAP), linearity (LIN), straightness (STR), and wobble (WOB) and on the distance value of spermatozoa amplitude lateral head displacement (ALH) and beat cross frequency (BCF). BRIN Publishing 2025-06-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/11410 10.55981/jbbi.2025.11410 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 1 (2025); 157-162 2548-611X 2442-2606 10.55981/jbbi.v12i1 eng https://ejournal.brin.go.id/JBBI/article/view/11410/9050 Copyright (c) 2025 Rio Ramadhan, Ananda, Hendri https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/11512 2025-12-22T06:48:57Z JBBI:ART

IMPROVEMENT OF SHEEP OOCYTE COMPETENCE IN VITRO THROUGH GLUTATHIONE ADDITION Yusmala, Mitha Supriatna, Iman Setiadi, Mohamad Agus fertilization glutathione in vitro maturation sheep oocyte Oocyte competence is a primary factor for the success of embryo production in vitro. This research aims to determine the effect of supplementation glutathione in sheep oocytes’ maturation and fertilization rates in vitro. In the first research, maturation oocytes were conducted in a medium supplemented with GSH at graded doses for 24 hours. In the second research, 1.0 mM GSH (the best dose from research I) was supplemented in the maturation medium, fertilization medium, and their combination for 14 hours. The higher oocyte maturation rates were significant (P < 0.05) in the GSH supplementation of 1.0 mM (87.00%). Furthermore, supplementation of GSH on the maturation medium resulted in a higher normal fertilization rate (56.43%) than in other groups. It is concluded that GSH supplementation on the maturation medium is more effective in increasing the competence of sheep oocytes to be mature and results in improved fertilization rates. BRIN Publishing 2025-12-13 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/11512 10.55981/jbbi.2025.11512 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 2 (2025); 177-186 2548-611X 2442-2606 10.55981/jbbi.v12i2 eng https://ejournal.brin.go.id/JBBI/article/view/11512/11086 Copyright (c) 2025 Mitha Yusmala, Iman Supriatna, Mohamad Agus Setiadi https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/11632 2025-12-22T06:49:13Z JBBI:ART

IDENTIFICATION OF SECONDARY METABOLITE COMPOUNDS USING GC-MS IN METHANOL EXTRACTS OF KEISSI TUBERS (Stephania venosa (Blume) Spreng) Salempa, Pince Salnus, Subakir Hasri Alkaloids GC-MS Identification Secondary metabolites Stephania venosa (Blume) Spreng Traditional medicinal plants are an important source of bioactive compounds that have the potential to be developed as raw materials for health products. One species used by the community in Mambi District, West Sulawesi, for cancer treatment is Belajang Keissi' (Stephania venosa (Blume) Spreng). This study aims to identify the secondary metabolites contained in the methanol extract of Belajang Keissi' tubers. The extraction process was carried out using the maceration method with methanol as the solvent, followed by qualitative phytochemical testing and compound profile analysis using GC-MS. The results of the phytochemical test showed that the methanol extract of Belajang Keissi' tubers contained alkaloids and flavonoids. Further GC-MS analysis revealed the presence of several dominant compounds, namely 9,12-octadecadienoic acid (Z,Z)-, methyl ester; trans-13-octadecenoic acid, methyl ester; methyl stearate; 6H-dibenzo[a,g]quinolizine, 5,8,13,13a-tetrahydro-2,3,9,10-tetramethoxy-; and (±)-2,3,3′,4′-tetramethoxy-α-methyl-5-(prop-1-enyl)stilbene. These findings indicate that Belajang Keissi tubers have potential as a source of bioactive compounds that can support the development of traditional medicines based on natural ingredients. BRIN Publishing 2025-12-13 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/11632 10.55981/jbbi.2025.11632 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 2 (2025); 187-193 2548-611X 2442-2606 10.55981/jbbi.v12i2 eng https://ejournal.brin.go.id/JBBI/article/view/11632/11087 Copyright (c) 2025 Pince Salempa, Subakir Salnus, Hasri https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/11634 2025-12-22T06:58:54Z JBBI:ART

ISOLATION OF ANTIBIOTIC PRODUCING MICROORGANISMS FROM CASSAVA ROOT TAPAI BY TRADITIONAL FERMENTED Muhiddin, Nurhayani H. Hidayah, Nurul Herawati, Netty Ramlawati Sukmadewi. M, Fitrah Darmayani , Dwi Marnia Idris, Nur Afni Yunita Antibiotic producing microorganisms Bacteria Cassava tapai Molds Yeasts Antibiotic resistance among pathogenic microorganisms has become a growing global health concern, necessitating the search for new natural sources of antimicrobial compounds. Cassava tapai, a traditional Indonesian fermented food, contains molds, yeasts, and bacteria with potential antibiotic-producing abilities. This study aimed to isolate and characterize antibiotic-producing microorganisms from cassava tapai fermented with local Sulawesi ‘Ragi’. Isolation, colony morphology characterization, and antibiotic activity tests were conducted using the Kirby–Bauer method against Escherichia coli ATCC 35218 and Staphylococcus aureus ATCC 25923. Inhibition zones were measured with a digital caliper, and data were analyzed using one-way ANOVA followed by Tukey’s HSD test or Kruskal–Wallis followed by the Mann–Whitney U test (p < 0.05). Three isolates Bb1, Bb2, and Wk3 exhibited significant antibiotic activity, with Bb1 showing stronger inhibition against E. Coli ATCC 35218. These results highlight confirms the potential of cassava tapai as a promising source of antibiotic-producing microorganisms for future antimicrobial discovery. BRIN Publishing 2025-12-13 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/11634 10.55981/jbbi.2025.11634 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 2 (2025); 258-271 2548-611X 2442-2606 10.55981/jbbi.v12i2 eng https://ejournal.brin.go.id/JBBI/article/view/11634/11101 Copyright (c) 2025 Nurhayani H. Muhiddin, Nurul Hidayah, Netty Herawati, Ramlawati, Fitrah Sukmadewi. M, Dwi Darmayani , Marnia, Nur Afni Yunita Idris https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/11917 2026-01-28T04:49:44Z JBBI:ART

THE ROLE OF AgNPs AND AB Mix IN THE GROWTH OF Aglaonema 'LADY VALENTINE' IN VITRO & EX VITRO Larrisa, Damasa Ines Nugrahani, Pangesti Wiyatiningsih, Sri Acclimatization AB Mix aglaonema AgNPs tissue culture Aglaonema ‘Lady Valentine’ is a popular ornamental plant with significant economic value. Conventional propagation methods have limitations, making tissue culture and acclimatization viable alternatives. However, low shoot multiplication and slow acclimatization remain major challenges. This study investigated the effects of silver nanoparticles (AgNPs) and AB Mix nutrient concentrations on in vitro and ex vitro plantlet growth using a two-factorial completely randomized design with four levels and three replications. The results showed varied growth responses, with the best performance observed in the 2 ppm AgNPs and 3 ml/L AB Mix treatment, which increased plantlet height, volume, and visual health. In ex vitro conditions, both 2 ppm and 3 ppm AgNPs combined with 3 ml/L AB Mix supported better plantlet adaptation, as seen in increased height, number of leaves and shoots, and seedling viability. Overall, the combination of 2 ppm AgNPs and 3 ml/L AB Mix proved most effective for enhancing multiplication and acclimatization BRIN Publishing 2026-01-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/11917 10.55981/jbbi.2025.11917 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 2 (2025); 450-455 2548-611X 2442-2606 10.55981/jbbi.v12i2 eng https://ejournal.brin.go.id/JBBI/article/view/11917/11546 Copyright (c) 2025 Damasa Ines Larrisa, Pangesti Nugrahani, Sri Wiyatiningsih https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/12206 2026-06-12T05:32:19Z JBBI:ART

CHARACTERISTICS AND KINEMATICS OF RAM SPERMATOZOA MOVEMENT IN FERTILIZATION MEDIUM USING SERICIN Andrian, Arindya Karja, Ni Wayan Kurniani Setiadi, Mohamad Agus Kaiin, Ekayanti Mulyawati BSA fertilization in vitro sericin spermatozoa This study aimed to evaluate the effectiveness of sericin on quality and kinematics of spermatozoa movement in fertilization medium and substituted for BSA in PEIV. Spermatozoa preparation was done with and without swim-up. Each group was incubated in fertilization medium with BSA, sericin 0.05%, 0.1%, 0.5%, and evaluated after addition of fertilization medium,15, 30, 60 minute. The results showed that the progressive motility values, sericin 0,5% group were higher than other groups (P<0.05). Membrane integrity showed differences between treatments. The VCL value showed no difference except for 0 and 30 minute swim-up group, sericin 0.5% was higher (P<0.05). VAP, VSL, LIN, and STR had no difference. The ALH at 30 minute swim-up and 60 minute without swim-up, sericin 0.5% were higher than others (P<0.05). In conclusion, the addition of sericin 0,5% has potential to replace BSA, sericin 0.5% can maintain quality and show early signs of hyperactivation of spermatozoa. BRIN Publishing 2026-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/12206 10.55981/jbbi.2026.12206 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 13 No. 1 (2026); 1-14 2548-611X 2442-2606 10.55981/jbbi.v13i1 eng https://ejournal.brin.go.id/JBBI/article/view/12206/12349 Copyright (c) 2026 Arindya Andrian, Ni Wayan Kurniani Karja, Mohamad Agus Setiadi, Ekayanti Mulyawati Kaiin https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/12608 2025-12-22T06:50:28Z JBBI:ART

CURRENT UNDERSTANDING IN THE ROLES OF WNT/RYK SIGNALING IN DEVELOPMENT AND HOMEOSTASIS Wijaya, Natalie Chandra Suryadi, Jimmy Yulandi, Adi embryonic development organ development ryk tissue homeostasis Wnt signaling Wnt/Ryk signaling represents a critical yet underexplored branch of the Wnt pathway, with significant roles in embryonic development and tissue homeostasis. While canonical Wnt/β-catenin signaling has been extensively studied, emerging research highlights Ryk as a key noncanonical Wnt co-receptor influencing processes such as convergent extension, organogenesis, stem cell regulation, and inflammation. Wnt/Ryk signaling mediates diverse cellular outcomes through interactions with ligands like Wnt5a and Wnt11 and exhibits complex crosstalk with canonical pathways. Its dysregulation has been linked to developmental disorders, neurodegenerative diseases, and cancer progression. This review summarizes current insights into Wnt/Ryk function during development and homeostasis, highlights its relevance in human health, and identifies open questions regarding its molecular mechanisms and therapeutic potential. A deeper understanding of this pathway may offer novel strategies for regenerative medicine and targeted treatments for Wnt-related diseases. BRIN Publishing 2025-12-13 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/12608 10.55981/jbbi.2025.12608 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 2 (2025); 241-249 2548-611X 2442-2606 10.55981/jbbi.v12i2 eng https://ejournal.brin.go.id/JBBI/article/view/12608/11100 Copyright (c) 2025 Natalie Chandra Wijaya, Jimmy Suryadi, Adi Yulandi https://creativecommons.org/licenses/by-nc-sa/4.0

oai:ejournal.brin.go.id:article/12920 2025-12-28T08:47:07Z JBBI:ART

UNVEILING THE ANTIDIABETIC POTENTIAL OF KEMUNING LEAVES (Murraya paniculata L. Jack): AN IN-SILICO EXPLORATION OF DPP-4 INHIBITORY ACTIVITY Ladika Wiratama, Arif Aulia Nanda, Taurisma Hidayah, Farsya Weningtyas, Anditri antidiabetes ADMET DPP-4 Molecular docking Murraya paniculata Type 2 diabetes mellitus is a global health issue associated with insulin resistance and impaired glucose metabolism. Murraya paniculata L. Jack (kemuning) has shown antidiabetic potential, yet its role as a DPP-4 inhibitor remains underexplored. This study aimed to investigate the potential of kemuning leaf bioactive compounds as natural DPP-4 inhibitors through an in-silico approach. Compound structures were obtained from the PubChem database and analyzed using AutoDock Vina for molecular docking against the DPP-4 protein. Pharmacokinetic properties, toxicity, and drug-likeness were evaluated using pkCSM and SwissADME, while complex stability was assessed through molecular dynamics simulation (RMSF). The results showed that most compounds demonstrated favorable binding affinities (−6.8 to −7.9 kcal/mol), along with acceptable pharmacokinetic and toxicity profiles. Among the tested compounds, 1-aminoindan-1,5-dicarboxylic acid emerged as the most promising candidate for natural DPP-4 inhibition. BRIN Publishing 2025-12-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://ejournal.brin.go.id/JBBI/article/view/12920 10.55981/jbbi.2025.12920 Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 12 No. 2 (2025); 272-285 2548-611X 2442-2606 10.55981/jbbi.v12i2 eng https://ejournal.brin.go.id/JBBI/article/view/12920/11108 Copyright (c) 2025 Arif Ladika Wiratama, Taurisma Aulia Nanda, Farsya Hidayah, Anditri Weningtyas https://creativecommons.org/licenses/by-nc-sa/4.0 b926b949c26bd13b958f47176a4d459e