HISTOLOGI LIMPA DAN HEMATOLOGI MENCIT YANG DIINFEKSI Escherichia coli SETELAH PEMBERIAN ASAM HUMAT GAMBUT KALIMANTAN
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Abstract
Senyawa asam humat mempunyai potensi imunostimulan. Penelitian ini bertujuan mengetahui pengaruh pemberian asam humat terhadap organ limpa mencit yang diinfeksi bakteri Escherichia coli. Penelitian ini menggunakan rancangan acak lengkap dengan enam perlakuan dan lima ulangan. Perlakuan tersebut yakni kontrol normal, kontrol negatif, kontrol positif isoprinosin, asam humat dosis 62,5; 125; dan 250 mg/kg berat badan (BB). Hasil penelitian menunjukkan bahwa infeksi bakteri E. coli pada mencit menyebabkan mencit mengalami gejala diare dan penurunan berat badan yang signifikan. Perbedaan signifikan (P<0,05) pada nilai hematokrit dan jumlah leukosit total perlakuan asam humat dan isoprinosin lebih tinggi dibandingkan kontrol negatif dan kontrol normal. Tidak terdapat perbedaan nyata (P>0,05) pada berat limpa mencit antar perlakuan, melalui pengamatan histologi ditemukan perbedaan ukuran histologi limpa mencit. Perlakuan asam humat 250 mg/kg BB mempunyai ukuran pulpa putih (495,8 ± 58,2µm) dan perlakuan asam humat 125 mg/kg BB mempunyai nilai leukosit tertinggi (6725 ± 1018 sel/mL). Pada pulpa merah perlakuan kontrol negatif ditemukan banyak sel limfosit yang menggerombol.
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