AKTIVITAS ANTIOKSIDAN EKSTRAK KAPANG ENDOFIT Cb.Gm.B3 ASAL RANTING KAYU MANIS (Cinnamomum burmanni)
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Abstract
Kayu manis (Cinnamomum burmanni) mengandung senyawa sinamaldehid yang memiliki aktivitas sebagai antioksidan kuat dan dapat menangkal radikal bebas. Dalam tanaman kayu manis terdapat kapang endofit yang hidup bersimbiosis. Kapang endofit dapat menghasilkan berbagai senyawa metabolit bioaktif termasuk antioksidan. Penelitian ini dilakukan untuk mengisolasi kapang endofit dari tanaman C. burmanni yang aktif sebagai antioksidan. Isolasi kapang endofit dilakukan menggunakan metode sterilisasi permukaan dan ditanam pada media PDA. Pengujian aktivitas antioksidan dilakukan menggunakan metode peredaman radikal bebas dengan reagen 2.2-difenil-1-pikrilhidrazil (DPPH). Isolat terpilih diidentifikasi secara molekuler untuk menentukan spesiesnya. Sebanyak 9 isolat kapang berhasil diisolasi dari jaringan ranting tanaman kayu manis. Aktivitas antioksidan tertinggi (IC50) didapatkan dari isolat Cb.Gm.B3 sebesar 13,219 ± 0,755 µg/mL. Isolat terpilih Cb.Gm.B3 secara taksonomi memiliki tingkat kemiripan yang tinggi dengan Neofusicoccum parvum isolat PEL23 (No. aksesi: KY053054.1).
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