PRODUKSI LIPASE DARI ISOLAT KAPANG HASIL MUTASI UNTUK TRANSESTERIFIKASI

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Published: Jun 19, 2019
Keywords:
ethyl methane sulfonate, kapang mutan, lipase, N-methyl-N’-nitro-N-nitrosoguanidine, ultraviolet

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Galih Cendana Nabilasani
Program Studi Bioteknologi, Sekolah Pascasarjana Institut Pertanian Bogor
Trismilah
Laboratorium Pengembangan Teknologi Industri Agro dan Biomedika, BPPT
Dadang Suhendar
Laboratorium Pengembangan Teknologi Industri Agro dan Biomedika, BPPT
Nisa Rachmania Mubarik
Departemen Biologi, FMIPA, Institut Pertanian Bogor

Abstract

Lipase dimanfaatkan salah satunya dalam produksi biodiesel, yaitu dalam reaksi transesterifikasi. Kernel B (KB) merupakan kapang yang diisolasi dari limbah inti dan biji kelapa sawit, yang menghasilkan lipase sebagai katalis dalam reaksi transesterifikasi. Namun aktivitas transesterifikasi yang dihasilkan oleh lipase dari KB lebih rendah dibandingkan dengan lipase komersial AK Amano. Tujuan penelitian ini adalah mendapatkan mutan kapang dengan aktivitas transesterifikasi yang lebih tinggi dibandingkan tipe liarnya (KB). Proses mutasi dilakukan dengan menggunakan sinar ultraviolet (UV), ethyl methane sulfonate (EMS), dan N-methyl-N’-nitro-N-nitrosoguanidine (NMNG) terhadap kapang KB. Mutasi KB dengan menggunakan sinar UV menghasilkan 11 isolat, dimana isolat dengan kode m4.1KB1 menghasilkan aktivitas transesterifikasi yang lebih tinggi dibandingkan tipe liar, yaitu 0,172 U·mg-1. Mutan m5.7KB, yang dihasilkan dari mutan m4.1KB1 dengan perlakuan EMS, mengalami penurunan aktivitas transesterifikasi hingga hanya sebesar 0,051 U·mg-1. Mutan m6.0,3KB2 hasil perlakuan NMNG mengalami peningkatan aktivitas transesterifikasi sebesar 91,2% lebih tinggi dari KB.

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Galih Cendana Nabilasani, Trismilah, Dadang Suhendar, & Nisa Rachmania Mubarik. (2019). PRODUKSI LIPASE DARI ISOLAT KAPANG HASIL MUTASI UNTUK TRANSESTERIFIKASI. Jurnal Bioteknologi Dan Biosains Indonesia, 6(1), 20–28. Retrieved from https://ejournal.brin.go.id/JBBI/article/view/1763
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Vol. 6 No. 1 (2019)
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Articles
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