PENGARUH SEREAL BERBAHAN SAGU DAN Moringa oleifera TERHADAP KADAR GLUKOSA DARAH TIKUS YANG DIINDUKSI ALOKSAN
Article Sidebar
Main Article Content
Abstract
Diabetes Mellitus (DM)tipe 2 dapat memicu stres oksidatif dan meningkatkan kadar glukosa darah puasa (GDP). Senyawa pati resisten dalam sereal siap saji Cersa Mori memiliki sifat antidiabetik. Penelitian ini bertujuan menganalisis pengaruh pemberian Cersa Mori terhadap kadar glukosa darah tikus putih diabetes yang diinduksi aloksan. Penelitian true-experimental ini menggunakan randomized pre-post control group design. Sampel sebanyak 27 ekor tikus jantan galur Wistar dibagi menjadi 3 kelompok secara acak yaitu; (KN) pakan dan akuades, (KP) glibenklamid 0,126 mg/200gBB/hari, (P) Cersa Mori 5g/200gBB/hari. KP sampai P diberikan aloksan 125 mg/KgBB secara subkutan dan intervensi dilakukan selama 30 hari. Pengukuran GDP menggunakan metode GOD-PAP. Hasil Uji-T menunjukkan pengaruh Cersa Mori dalam menurunkan GDP tikus hiperglikemia (P = 0,006). Uji ANOVA satu arah menunjukkan penurunan GDP pada kelompok Cersa Mori (P) setara dengan tikus yang diberi glibenklamid (P = 0,366). Dengan demikian dapat disimpulkan bahwa pemberian Cersa Mori dosis 5g/200gBB/hari selama 30 hari berpengaruh terhadap penurunan GDP secara signifikan.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
a). Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Attribution-NonCommercial-ShareAlike 4.0 International that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b). Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
References
Arjadi F, Mustofa M (2017) Ektrak daging buah mahkota dewa meregenerasi sel Pulau Langerhans pada tikus putih diabetes. Biogenesis 5: 27-33. doi: 10.24252/bio.v5i1.3430
Asbar R, Sugiyono S, Haryanto B (2014) Peningkatan pati resisten tipe III pada tepung singkong modifikasi (Mocaf) dengan perlakuan pemanasan-pendinginan berulang dan aplikasinya pada pembuatan mie kering. J Pangan 23: 157-165. doi: 10.33964/jp.v23i2.60
Astawan M (2019) Pengaruh konsumsi tempe dari kedelai germinasi dan non-germinasi terhadap profil darah tikus diabetes. J Pangan 28:135-44. doi: 10.33964/jp.v28i2.439
Bachmid N, Sangi MS, Pontoh JS (2015) Uji aktivitas antikolesterol ekstrak etanol daun patikan emas (Euphorbia prunifolia Jacq.) pada tikus wistar yang hiperkolesterolemia. J Mipa 4: 29-35. doi: 10.35799/jm.4.1.2015.6901
den Besten G, van Eunen K, Groen AK, Venema K, Reijngoud DJ, Bakker BM (2013) The role of short-chain fatty acids in the interplay between diet, gut microbiota, and host energy metabolism. J Lipid Res 54: 2325–2340. doi: 10.1194/jlr.R036012
Diasys (2014) Glucose Hexokinase FS: diagnostic reagent for quantitive in vitro determination of glucose in serum or plasma on photometric systems. Diasys Diagnostic Systems Gmbh, Holzheim, Germany
Birt DF, Boylston T, Hendrich S, Jane JL, Hollis J, Li L, McClelland J, Moore S, Phillips GJ, Rowling M, Schalinske K, Scott MP, Whitley EM (2013) Resistant starch: promise for improving human health. Adv Nutr 4: 587-601. doi: 10.3945/an.113.004325
Ekafitri R (2017) Pati resisten pada beras: jenis, metode peningkatan, efek untuk kesehatan, dan aplikasinya. J Pangan 26: 1-15. doi: 10.33964/jp.v26i3.362
Fauziyah A, Marliyati SA, Kustiyah L (2017) Substitusi tepung kacang merah meningkatkan kandungan gizi, serat pangan dan kapasitas antioksidan beras analog sorgum. J Gizi Pangan 12: 147-152. doi: 10.25182/jgp.2017.12.2.147-152
Federer WT (1991) Statistics and Society: Data Collection and Interpretation. Second edition. Marcel Dekker, New York
Firdaus J, Sulistyaningsih E, Subagio A (2018) Resistant starch modified cassava flour (Mocaf) improves insulin resistance. Asian J Clinic Nutr 10: 32-36. doi: 10.3923/ajcn.2018.32.36
Fitria L, Mulyati, Tiraya CM, Budi AS (2015) Profil reproduksi jantan tikus (Rattus norvegicus Berkenhout, 1769) galur wistar stadia muda, pradewasa, dan dewasa. J Biol Papua 7: 29-36. doi: 10.31957/jbp.429
Giacco R, De Giulio B, Vitale M, Cozzolino R (2013) Functional foods: can food technology help in the prevention and treatment of diabetes? Food Nutr Sci 4: 827-837. doi: 10.4236/fns.2013.48108
Goñi I, Garcia-Diz L, Mañas E, Saura-Calixto F (1996) Analysis of resistant starch: A method for foods and food products. Food Chem 56: 445-449. doi: 10.1016/0308-8146(95)00222-7
Gurjar HPS, Irchhaiya R, Pandey H, Singh PP (2018) Assessment of antidiabetic potential of leaf extract of Bauhinia variegata Linn. in type-I and type-II diabetes. Int J Green Pharmacy 12: 401-409. doi: 10.22377/ijgp.v12i02.1793
Hajiaghaalipour F, Khalilpourfarshbafi M, Arya A (2015) Modulation of glucose transporter protein by dietary flavonoids in type 2 diabetes mellitus. Int J Biol Sci 11: 508-524. 11:508-524. doi: 10.7150/ijbs.11241
Hariyanto B, Cahyana PT, Putranto AT, Wahyuningsih SB, Marsono Y (2017) Penggunaan beras sagu untuk penderita pradiabetes. J Pangan 26: 127-136. doi: 10.33964/jp.v26i2.361
Haub MD, Hubach KL, Al-tamimi EK, Ornelas S, Seib PA (2010) Different types of resistant starch elicit different glucose reponses in humans. J Nutr Metabolism 2010: ID 230501. doi: 10.1155/2010/230501
Im Walde SS, Dohle C, Schott-Ohly P, Gleichmann H (2002) Molecular target structures in alloxan-induced diabetes in mice. Life Sci 71: 1681-1694. doi: 10.1016/S0024-3205(02)01918-5
Irdalisa I, Safrida S, Khairil K, Abdullah A, Sabri M (2015) Profil kadar glukosa darah pada tikus setelah penyuntikan aloksan sebagai hewan model hiperglikemik. J Edubio Tropika 3: 25-28
Joseph OI, Nnabuihe ED, Ukoha U, Ezinwa AC (2017) The effects of cassava starch on the pancreas of adult wistar rats. J Med Physiol Biophys 31: 9-15
Jyoshna E, Hymavathi TV (2017) Review of studies on effect of resistant starch supplementation on glucose and insulin. J Pharmacog Phytochem 6: 55-58
Laila W (2018) Pengaruh pemberian sagu (Metroxylon spp) terhadap kadar glukosa darah mencit putih jantan (Mus musculus) diabetes melitus. Scientia J Far Kes 8: 193-199. doi: 10.36434/scientia.v8i2.169
Mans K, Aburjai T (2019) Accessing the hypoglycemic effects of seed extract from celery (Apium graveolens) in alloxan-induced diabetic rats. J Pharmaceutical Res Int 26: 1-10. doi: 10.9734/jpri/2019/v26i630152
Mashi JA, Sa’id AM, Idris RI, Aminu I, Muhammad AA, Inuwa IM (2019) Persea Americana leaf ethyl acetate extract phytochemical, in-vitro antioxidant and in-vivo potentials to mitigate oxidative stress in alloxan-induced hyperglycaemic rats. Asian Plant Res J 2: 1-11. doi: 10.9734/APRJ/2019/v2i230041
Miao X, Gu Z, Liu Y, Jin M, Lu Y, Gong Y, Li L, Li C (2018) The glucagon-like peptide-1 analogue liraglutide promotes autophagy through the modulation of 5′-AMP-activated protein kinase in INS-1 β-cells under high glucose conditions. Peptides 100: 127-139. doi: 10.1016/j.peptides.2017.07.006
Morrison DJ, Preston T (2016) Formation of short chain fatty acids by the gut microbiota and their impact on human metabolism. Gut Microbes 7: 189-200. doi: 10.1080/19490976.2015.1134082
Nasution DM, Parwata IMOA, Suirta IW, Wasudewa KM (2018) Efektifitas ekstrak air daun gaharu (Gyrinop versteegii) dalam menurunkan kadar glukosa darah pada tikus wistar hiperglikemia. J Media Sains 2: 83-89. doi: 10.36002/jms%203.v2i2.427
Palguna IGPA, Sugiyono S, Haryanto B (2013) Optimasi rasio pati terhadap air dan suhu gelatinisasi untuk pembentukan pati resisten tipe III pada pati sagu (Metroxylon sagu). J Pangan 22: 253-262. doi: 10.33964/jp.v22i3.107
PERKENI (2015) Konsensus: Pengelolaan dan Pencegahan Diabetes Melitus Tipe 2 di Indonesia. Perkumpulan Endokrinologi Indonesia. PB PERKENI, Jakarta.
Pitriya IA, Rahman N, Sabang SM (2017) Efek ekstrak buah kelor (Moringa oleifera) terhadap penurunan kadar gula darah mencit (Mus musculus). J Akad Kim 6: 35-42
Pujiastuti E, Amilia D (2018) Efektivitas ekstrak etanol daun kenikir (Cosmos caudatus kunth) terhadap penurunan kadar glukosa darah pada tikus putih galur wistar yang diinduksi aloksan. Cendekia J Pharm 2: 16-21. doi: 10.31596/cjp.v2i1.13
Rachmah AR, Harahap U, Hasibuan PAZ (2018) Pengaruh ekstrak etanol kulit buah nanas (Ananas comosus (L.) Merr.) terhadap glukosa darah pada mencit hiperglikemia secara in vivo. J ’Aisyiyah Med 2: 18-27. doi: 10.36729/jam.v2i1.66
Ramracheya R, Chapman C, Chibalina M, Dou H, Miranda C, González A, Moritoh Y, Shigeto M, Zhang Q, Braun M, Clark A, Johnson PR, Rorsman P, Briant LJB (2018) GLPâ€1 suppresses glucagon secretion in human pancreatic alphaâ€cells by inhibition of P/Qâ€type Ca2+ channels. Physiol Rep 6: 1-17. doi: 10.14814/phy2.13852
Ridwan E (2013) Etika pemanfaatan hewan percobaan dalam penelitian kesehatan. J Indones Med Assoc 63: 112-116
Badan Litbangkes (2013) Riset kesehatan dasar (RISKESDAS) 2013. Laporan Nasional 2013. Badan Penelitian dan Pengembangan Kesehatan, Kemenkes RI
Robertson MD, Wright JW, Loizon E, Debard C, Vidal H, Shojaee-Moradie F, Russell-Jones D, Umpleby AM (2012) Insulin-sensitizing effects on muscle and adipose tissue after dietary fiber intake in men and women with metabolic syndrome. J Clinic Endocrinol Metabolism 97: 3326-3332. doi: 10.1210/jc.2012-1513
Setiarto RHB, Jenie BSL, Faridah DN, Saskiawan I (2015) Kajian peningkatan pati resisten yang terkandung dalam bahan pangan sebagai sumber prebiotik. J Ilmu Pertanian Indones 20: 191-200. doi: 10.18343/jipi.20.3.191
Setiarto RHB, Widhyastuti N, Sumariyadi A (2018) Peningkatan kadar pati resisten tipe III tepung singkong termodifikasi melalui fermentasi dan pemanasan bertekanan-pendinginan. Biopropal Industri 9: 9-23. doi: 10.36974/jbi.v9i1.3425
Uthumporn U, Wahidah N, Karim AA (2014) Physicochemical properties of starch from sago (Metroxylon sagu) palm grown in mineral soil at different growth stages. IOP Conf Ser: Mater Sci Eng 62: 012026. doi: 10.1088/1757-899X/62/1/012026
Vargas-Aguilar P (2016) Flours and starches made from cassava (yuca), yam, sweet potatoes and ñampi: functional properties and possible applications in the food industry. Revista Tecnología en Marcha 29: 86-94. doi: 10.18845/tm.v29i5.2519
Visuthranukul C, Sirimongkol P, Prachansuwan A, Pruksananonda C, Chomtho S (2015) Low-glycemic index diet may improve insulin sensitivity in obese children. Pediatric Res 78: 567-573. doi: 10.1038/pr.2015.142
Wahjuningsih SB, Marsono Y, Praseptiangga D, Haryanto B (2016) Resistant starch content and glycaemic index of sago (Metroxylon spp.) starch and red bean (Phaseolus vulgaris) based analogue rice. Pak J Nutr 15: 667-672. doi: 10.3923/pjn.2016.667.672
Wahjuningsih SB, Haslina, Untari S, Wijanarka A (2018b) Hypoglycemic effect of analog rice made from modified cassava flour (Mocaf), arrowroot flour and kidney bean flour on STZ-NA induced diabetic rats. Asian J Clin Nutr 10: 8-15. doi: 10.3923/ajcn.2018.8.15
Wahjuningsih SB, Septiani AR, Haslina H (2018a) Organoleptik cereal dari tepung beras merah (Oryza nivara Linn.) dan tepung kacang merah (Phaseolus vulgaris Linn.). J Litbang Provinsi Jawa Tengah 16: 131-142. doi: 10.36762/litbangjateng.v16i2.758
WHO (2017) Diabetes fact sheet. World Health Organization. Geneva (CH). Switzerland
Wu Y, Ding Y, Tanaka Y, Zhang W (2014) Risk factors contributing to type 2 diabetes and recent advances in the treatment and prevention. Int J Med Sci 11: 1185-1200. doi: 10.7150/ijms.10001
Yalçın T, Al A, RakıcıoÄŸlu N (2017) The effects of meal glycemic load on blood glucose levels of adults with different body mass indexes. Indian J Endocrinol Metab 21: 71-75. doi: 10.4103/2230-8210.195995
Zhang H, Jin Z (2011) Preparation of resistant starch by hydrolysis of maize starch with pullulanase. Carbohydr Polym 83: 865-867. doi: 10.1016/j.carbpol.2010.08.066
Zhou J, Keenan MJ, Keller J, Fernandezâ€Kim SO, Pistell PJ, Tulley RT, Raggio AM, Shen L, Zhang H, Martin RJ, Blackman MR (2012) Tolerance, fermentation, and cytokine expression in healthy aged male C57BL/6J mice fed resistant starch. Mol Nutr Food Res 56: 515-518. doi: 10.1002/mnfr.201100521