KONSENTRASI MALONDIALDEHID PADA TIKUS DIABETIK YANG DIBERI PAKAN BERBAHAN SAGU (Metroxylon sagu) DAN Moringa oleifera
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Abstract
Hiperglikemia pada diabetes melitus tipe 2 (DMT2) dapat menimbulkan stres oksidatif yang ditandai dengan peningkatan produksi malondialdehid (MDA). Flavonoid memiliki aktivitas antioksidan yang mampu menekan produksi radikal bebas penyebab stres oksidatif. Flavonoid terdapat di dalam produk pangan Cersa mori (CM) yang terbuat dari sagu (Metroxylon sagu) dan kelor (Moringa oleifera). Penelitian ini bertujuan menganalisis pengaruh pemberian CM terhadap kadar MDA tikus diabetes yang diinduksi aloksan. Aktivitas antioksidan dan total flavonoid CM masing-masing dianalisis menggunakan metode reduksi DPPH dan kolorimetri.Tiga puluh dua ekor tikus Wistar dibagi menjadi 4 kelompok: kontrol negatif (K1), kontrol positif 1 (K2), kontrol positif 2 (K3), dan perlakuan yang diberikan 5 g CM/200 g BB per hari (K4). Intervensi dilaksanakan selama 30 hari. Kadar MDA diperiksa sebelum dan setelah intervensi secara spektrofotometri. Hasil menunjukkan adanya penurunan signifikan kadar MDA pada K2, K3 dan K4 yaitu secara berturut-turut sebesar -11,5 ± 3,39 µM; -10,5 ± 4,32 µM; dan -14,9 ± 2,85 µM. Penurunan kadar MDA terendah terdapat pada K4 yang diberikan CM (p < 0,05).
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