PROFIL HEMATOLOGI TIKUS (Rattus norvegicus) GALUR SPRAGUE-DAWLEY JANTAN UMUR 7 DAN 10 MINGGU
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Abstract
Saat ini belum banyak tersedia informasi profil hematologi untuk hewan coba tikus yang dikembangbiakkan di Indonesia. Kebanyakan peneliti menggunakan data profil hematologi dari referensi luar Indonesia. Penelitian ini bertujuan untuk mendapatkan data hematologi tikus jantan usia 7 dan 10 minggu. Hewan coba yang digunakan adalah tikus jantan galur Sprague-Dawley yang diperoleh dari Laboratorium Hewan Percobaan-BPOM. Sebanyak 60 ekor tikus jantan yang terdiri dari 30 ekor usia 7 minggu dan 30 ekor usia 10 minggu diambil darahnya melalui sinus orbitalis. Selanjutnya, dianalisa jumlah sel eritrosit, hemoglobin, hematokrit, MCV, MCH, MCHC, leukosit. Hasil analisis statistik menunjukkan terdapat perbedaan signifikan antara tikus jantan usia 7 dan 10 minggu untuk parameter jumlah eritrosit, hemoglobin, hematokrit, MCV, MCHC dan leukosit. Tetapi tidak terdapat perbedaan signifikan untuk jumlah MCH, trombosit dan berat badan tikus. Data yang diperoleh dapat digunakan sebagai referensi penelitian yang berhubungan erat dengan profil hematologi dan penyakit kelainan darah lainnya.
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