INHIBITORY ACTIVITY OF INDONESIAN MICROBIAL EXTRACTS AGAINST PROLIFERATION OF DLD-1 COLORECTAL CANCER CELL LINE
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Abstract
Colorectal cancer (CRC) is the second deadliest cancer in the world. Several anti-cancer agents are currently used for the clinical treatment of CRC. However, toxicity and drug resistance pose significant challenges in CRC chemotherapy. On the other hand, microbe-derived natural products have been explored as a source for the development of anti-cancer therapeutic agents. This study aimed to examine the potential of the microbial library in BioMCC (Biotech Center-BPPT Microbial Culture Collection) as a source for anti-cancer drug discovery. Among the 720 fungal extracts tested, 60 extracts (8.3%) showed inhibitory activity against the proliferation of the colorectal carcinoma DLD-1 cell line, while not affecting Vero cells (African green monkey kidney normal cell line). One of these active extracts was derived from the fungus Sporothrix sp. BioMCC-f.T.7716. Although the inhibitory mechanism of this extract against the proliferation of the DLD-1 cell line could not be determined, this study clearly demonstrated the potential use of Indonesian microbial extracts as a source for the discovery of anti-cancer agents.
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References
Abbady AQ, Twair A, Ali B, Murad H (2017) Characterization of annexin V fusion with the superfolder GFP in liposomes binding and apoptosis detection. Front Physiol 8:1–12. doi: 10.3389/fphys.2017.00317
Abraham M, Augustine D, Rao RS, Sowmya S V., Haragannavar VC, Nambiar S, Prasad K, Awan KH, Patil S (2017) Naturally Available Extracts Inhibiting Cancer Progression: A Systematic Review. J Evidence-Based Complement Altern Med 22:870–878. doi: 10.1177/2156587217744914
Almeida-Paes R, Frases S, Fialho Monteiro PC, Gutierrez-Galhardo MC, Zancopé-Oliveira RM, Nosanchuk JD (2009) Growth conditions influence melanization of Brazilian clinical Sporothrix schenckii isolates. Microbes Infect 11:554–562. doi: 10.1016/j.micinf.2009.03.002
Bukowski K, Kciuk M, Kontek R (2020) Mechanisms of multidrug resistance in cancer chemotherapy1. Bukowski K, Kciuk M, Kontek R. Mechanisms of multidrug resistance in cancer chemotherapy. Int J Mol Sci. 2020;21(9). doi:10.3390/ijms21093233. Int J Mol Sci 21
Chakraborty S, Rahman T (2012) The difficulties in cancer treatment. 1–5. doi: 10.3332/ecancer.2012.ed16
Choi HY, Ahn JH, Kwon H, Yim JH, Lee D, Choi JH (2022) Citromycin Isolated from the Antarctic Marine-Derived Fungi, Sporothrix sp., Inhibits Ovarian Cancer Cell Invasion via Suppression of ERK Signaling. Mar Drugs 20. doi: 10.3390/md20050275
Cragg GM, Pezzuto JM (2016) Natural Products as a Vital Source for the Discovery of Cancer Chemotherapeutic and Chemopreventive Agents. Med Princ Pract 25:41–59. doi: 10.1159/000443404
de Hoog S (1995) Atlas of Clinical Fungi. Centraalbureau voor Schimmelcultures, Baarn and Delft
Ferlay J, Ervik M, Lam F, Colombet M, Mery L, Piñeros M, Znaor A, Soerjomataram I, Bray F (2020) Global Cancer Observatory: Cancer Today. https://gco.iarc.fr/today. Accessed 1 Nov 2022 Red Flower and Investigation of the Antioxidant ,. Antibiotics 11. doi: 10.3390/antibiotics11111652
Glimelius B, Tiret E, Cervantes A, Arnold D (2013) Rectal cancer: ESMO clinical practice guidelines for diagnosis, treatment and follow-up. Ann Oncol 24. doi: 10.1093/annonc/mdt240
Holohan C, Van Schaeybroeck S, Longley DB, Johnston PG (2013) Cancer drug resistance: an evolving paradigm. Nat Rev Cancer 13:714–726. doi: 10.1038/nrc3599
Huang X, Yang Z, Xie Q, Zhang Z, Zhang H, Ma J (2019) Natural products for treating colorectal cancer: A mechanistic review. Biomed Pharmacother 117:109142. doi: 10.1016/j.biopha.2019.109142
Hwang J, Yi M, Zhang X, Xu Y, Jung JH, Kim DK (2013) Cytochalasin B induces apoptosis through the mitochondrial apoptotic pathway in HeLa human cervical carcinoma cells. Oncol Rep 30:1929–1935. doi: 10.3892/or.2013.2617
Kinghorn AD, Carcache De Blanco EJ, Lucas DM, Liva Rakotondraibe H, Orjala J, Doel Soejarto D, Oberlies NH, Pearce CJ, Wani MC, Stockwell BR, Burdette JE, Swanson SM, Fuchs JR, Phelps MA, Xu L, Zhang X, Shen YY, Kinghorn D (2016) Discovery of Anticancer Agents of Diverse Natural Origin Correspondence to: A HHS Public Access
Kornienko A, Evidente A, Vurro M, Mathieu V, Cimmino A, Evidente M, van Otterlo WAL, Dasari R, Lefranc F, Kiss R (2015) Toward a Cancer Drug of Fungal Origin. Med Res Rev 35:937–967. doi: 10.1002/med.21348
Kunicka T, Prochazka P, Krus I, Bendova P, Protivova M, Susova S, Hlavac V, Liska V, Novak P, Schneiderova M, Pitule P, Bruha J, Vycital O, Vodicka P, Soucek P (2016) Molecular profile of 5-fluorouracil pathway genes in colorectal carcinoma. BMC Cancer 16:1–12. doi: 10.1186/s12885-016-2826-8
Kwon-Chung KJ, Bennett KJ (1992) Medical Mycology. Lea & Febiger, Philadelphia
Lekshmi A, Varadarajan SN, Lupitha SS, Indira D, Ann Mathew K, Nair AC, Nair M, Prasad T, Sekar H, Gopalakrishnan AK, Murali A, Santhoshkumar TR (2017) A quantitative real-time approach for discriminating apoptosis and necrosis. Cell Death Discov 3:1–10. doi: 10.1038/cddiscovery.2016.101
Lindskog EB, Derwinger K, Gustavsson B, Falk P, Wettergren Y (2014) Thymidine phosphorylase expression is associated with time to progression in patients with metastatic colorectal cancer. BMC Clin Pathol 14:1–7. doi: 10.1186/1472-6890-14-25
Lopez A, Reyna DE, Gitego N, Kopp F, Zhou H, Miranda-Roman MA, Nordstrøm LU, Narayanagari SR, Chi P, Vilar E, Tsirigos A, Gavathiotis E (2022) Co-targeting of BAX and BCL-XL proteins broadly overcomes resistance to apoptosis in cancer. Nat Commun 13. doi: 10.1038/s41467-022-28741-7
Mateus DN, Willian LC, Jos MR da L, Maria CMK (2014) Lithium chloride affects mycelial growth of white rot fungi: Fungal screening for Li-enrichment. African J Microbiol Res 8:2111–2123. doi: 10.5897/ajmr2014.6619
Mehta D, Kumar Mehta B (2014) Natural Products: Source of Potential Drugs. African J Basic Appl Sci. https://doi.org/10.5829/idosi.ajbas.2014.6.6.21983
Moehler M, Göpfert K, Lenz H-J (2018) Outlook: Immunotherapy in Gastrointestinal Carcinoma - Innovative Strategies. Oncol Res Treat 41:313–315. doi: 10.1159/000489047
Ramírez-Soto MC, Aguilar-Ancori EG, Tirado-Sánchez A, Bonifaz A (2018) Ecological determinants of sporotrichosis etiological agents. J Fungi 4:1–11. doi: 10.3390/jof4030095
Richter DL, Robinson SC, Beardslee MP, Habarth ML (2008) Differential sensitivity of fungi to lithium chloride in culture media. Mycol Res 112:717–724. doi: 10.1016/j.mycres.2008.01.013
Rodrigues AM, de Hoog GS, de Camargo ZP (2016) Sporothrix Species Causing Outbreaks in Animals and Humans Driven by Animal–Animal Transmission. PLoS Pathog 12:1–7. doi: 10.1371/journal.ppat.1005638
Sakowicz-Burkiewicz M, Przybyla T, Wesserling M, Bielarczyk H, Maciejewska I, Pawelczyk T (2016) Suppression of TWIST1 enhances the sensitivity of colon cancer cells to 5-fluorouracil. Int J Biochem Cell Biol 78:268–278. doi: 10.1016/j.biocel.2016.07.024
Sawicka D, Hryniewicka A, Gohal S, Sadowska A, Pryczynicz A, Guzinska-Ustymowicz K, Sokolowska E, Morzycki JW, Car H (2022) Establishment of In Vitro and In Vivo Anticolorectal Cancer Efficacy of Lithocholic Acid-Based Imidazolium Salts. Int J Mol Sci 23. doi: 10.3390/ijms23137019
Sigler L, Harris JL, Dixon DM, Flis AL, Salkin IF, Kemna M, Duncan RA (1990) Microbiology and potential virulence of Sporothrix cyanescens, a fungus rarely isolated from blood and skin. J Clin Microbiol 28:1009–1015. doi: 10.1128/jcm.28.5.1009-1015.1990
The American Cancer Society (2020) Colorectal Cancer Early Detection, Diagnosis, and Staging. www.cancer.org/cancer/colon-rectal-cancer/about/key-statistics.html. Accessed 1 Nov 2022
Varghese E, Samuel SM, Sadiq Z, Kubatka P, Liskova A, Benacka J, Pazinka P, Kruzliak P, Büsselberg D (2019) Anti-Cancer Agents in Proliferation and Cell Death: The Calcium Connection. Int J Mol Sci 20:3017. doi: 10.3390/ijms20123017
Vodenkova S, Buchler T, Cervena K, Veskrnova V, Vodicka P, Vymetalkova V (2020) 5-fluorouracil and other fluoropyrimidines in colorectal cancer: Past, present and future. Pharmacol Ther 206:107447. doi: 10.1016/j.pharmthera.2019.107447
von Rintelen K, Arida E, Häuser C (2017) A review of biodiversity-related issues and challenges in megadiverse Indonesia and other Southeast Asian countries. Res Ideas Outcomes 3. doi: 10.3897/rio.3.e20860
Waluyo D, Prabandari EE, Pramisandi A, Hidayati DN, Chrisnayanti E, Puspitasari DJ, Dewi D, Suryani, Kristiningrum, Oktaviani AN, Afrianti KR, Nonaka K, Matsumoto A, Tokiwa T, Adipratiwi N, Ariyani T, Hartuti ED, Putri TZ, Rahmawati Y, Inaoka DK, Miyazaki Y, Sakura T, Nurlaila, Siska E, Kurnia K, Bernawati P, Melinda, Mahsunah AH, Nugroho NB, Mori M, Dobashi K, Yamashita M, Nurkanto A, Watanabe A, Shiomi K, Wibowo AE, Nozaki T (2021) Exploring natural microbial resources for the discovery of anti-malarial compounds. Parasitol Int 85. doi: 10.1016/j.parint.2021.102432
Xi Y, Xu P (2021) Global colorectal cancer burden in 2020 and projections to 2040. Transl Oncol 14:101174. doi: 10.1016/j.tranon.2021.101174
Yu J, Zhong B, Xiao Q, Du L, Hou Y, Sun H-S, Lu J-J, Chen X (2020) Induction of programmed necrosis: A novel anti-cancer strategy for natural compounds. Pharmacol Ther 214:107593. doi: 10.1016/j.pharmthera.2020.107593
Zhang JH, Chung TDY, Oldenburg KR (1999) A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 4:67–73. doi: 10.1177/108705719900400206
Zhong L, Li Y, Xiong L, Wang W, Wu M, Yuan T, Yang W, Tian C, Miao Z, Wang T, Yang S (2021) Small molecules in targeted cancer therapy: advances, challenges, and future perspectives. Signal Transduct Target Ther 6:201. doi: 10.1038/s41392-021-00572-w
Zhou X, Rodrigues AM, Feng P, De Hoog GS (2014) Global ITS diversity in the Sporothrix schenckii complex. Fungal Divers 66:153–165. doi: 10.1007/s13225-013-0220-2