Synthesis of L-Lactic Acid from Fermentation of Cassava Pulp by Using Tempeh Inoculum
Keywords:
Cassava waste pulp, Lactic acid, Tempeh inoculum, R.S.M. Box-BehnkenAbstract
This study used cassava waste pulp as a fermentation substrate to produce lactic
acid using a tempeh inoculum. Tempeh inoculum is a mixed culture of Rhizopus with Rhizopus
oligosporus as the primary fungus. Lactic acid is an organic acid most widely used in the food,
pharmaceutical, cosmetic and chemical industries. One of the important uses of lactic acid is as a raw
material for producing Polylactic Acid (PLA) biopolymers, namely polymers that can decompose
naturally in a relatively fast time. The analysis was performed using the Response Surface
Methodology (RSM) method and the Box Behnken Design (BBD) experimental design with substrate
concentration parameters, inoculum concentration, and incubation time on lactic acid. The
fermentation process is carried out using a flask shaker at a temperature of 30 ºC, pH 6.0, and a
rotational speed of 150 rpm. The optimum yield for lactic acid is 6.65 g/L. It was acquired at substrate
20 g/L, inoculum concentration 0.30 % (w/v) at an incubation time of 72 hours.
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References
M. A. Abdel-Rahman et al., “One-factor-at-a-time and response surface statistical designs for improved lactic acid
production from beet molasses by Enterococcus hirae ds10,” SN Appl. Sci., vol. 2, no. 4, pp. 1–14, 2020.
T. Casalini, F. Rossi, A. Castrovinci, and G. Perale, “A Perspective on Polylactic Acid-Based Polymers Use for
Nanoparticles Synthesis and Applications,” Front. Bioeng. Biotechnol., vol. 7, no. October, pp. 1–16, 2019.
G. Li et al., “Synthesis and Biological Application of Polylactic Acid,” Molecules, vol. 25, no. 21, p. 5023, Oct.
P. Febriyanto and A. W. Satria, “Kajian Awal Pemanfaatan Limbah Onggok Sebagai Substitusi Batubara,” vol. 8,
no. 1, pp. 14–18, 2019.
Sutikno, Marniza, Selviana, and N. Musita, “Pengaruh konsetrasi enzim selulase, α–amilase dan glukoamilase
terhadap kadar gula reduksi dari onggok,” J. Teknol. Ind. dan Hasill Pertan., vol. 21, no. 1, pp. 1–12, 2016.
H. Chen et al., “Efficient lactic acid production from cassava bagasse by mixed culture of Bacillus coagulans and
lactobacillus rhamnosus using stepwise pH controlled simultaneous saccharification and co-fermentation,” Ind.
Crops Prod., vol. 146, no. 15, p. 112175, 2020.
J. V. Carpinelli Macedo, F. F. de Barros Ranke, B. Escaramboni, T. S. Campioni, E. G. Fernández Núñez, and P. de
Oliva Neto, “Cost-effective lactic acid production by fermentation of agro-industrial residues,” Biocatal. Agric.
Biotechnol., vol. 27, no. July, p. 101706, 2020.
J. Romo-Buchelly, M. Rodríguez-Torres, and F. Orozco-Sánchez, “Biotechnological valorization of agro industrial
and household wastes for lactic acid production,” Rev. Colomb. Biotecnol., vol. 21, no. 1, pp. 113–127, 2019.
Y. Sine and E. S. Soetarto, “Isolasi dan identifikasi kapang Rhizopus pada tempe gude (Cajanus cajan L.),” Savana
Cendana, vol. 3, no. 04, pp. 67–68, 2018.
Y. Q. Fu, L. F. Yin, H. Y. Zhu, and R. Jiang, “High-efficiency L-lactic acid production by Rhizopus oryzae using a
novel modified one-step fermentation strategy,” Bioresour. Technol., vol. 218, pp. 410–417, 2016.
A. S. Azmi, N. Yusuf, D. N. Jimat, and N. I. M. Puad, “Co-production of lactic acid and ethanol using rhizopus sp.
from hydrolyzed inedible cassava starch and leaves,” IIUM Eng. J., vol. 17, no. 2, pp. 1–10, 2016.
T. Yuwa-amornpitak and K. Chookietwatana, “Bioconversion of waste cooking oil glycerol from cabbage extract
to lactic acid by Rhizopus microsporus,” Brazilian J. Microbiol., vol. 49, pp. 178–184, 2018.
D. Junaidi, M. C. K. P. Santoso, E. S. Retnoningtyas, and S. B. Hartono, “Penurunan kadar sianida pada umbi
gadung (Dioscorea hispida) dengan proses fermentasi menggunakan kapang Rhizopus Oryzae,” Widya Tek., vol.
, no. 1, pp. 9–14, 2018.
R. Kumar and S. Shivakumar, “Production of L-Lactic acid from starch and food waste by amylolytic Rhizopus
oryzae MTCC 8784,” Int. J. ChemTech Res., vol. 6, no. 1, pp. 527–537, 2014.
V. Sridevi, M. Padmaja, A. Sahitya, N. Vardhan, and G. Rao, “Application of Box-Behnken Design for the
Optimized Production of Lactic Acid by Newly Isolated Lactobacillus plantarum JX183220 Using Cassava
(Manihot esculenta Crantz) Flour,” Br. Biotechnol. J., vol. 9, no. 2, pp. 1–9, 2015.
R. C. Orlandelli, M. S. Santos, J. C. Polonio, J. L. de Azevedo, and J. A. Pamphile, “Use of agro-industrial wastes
as substrates for α-amylase production by endophytic fungi isolated from Piper hispidum Sw,” Acta Sci. - Technol.,
vol. 39, no. 3, pp. 255–261, 2017.
T. Payot and M. Fick, “On-line estimation of lactic acid concentration by conductivity measurement in fermentation
broth,” Biotechnol. Tech., vol. 11, no. 1, pp. 17–20, 1997.
S. H. Cheong, “Physicochemical properties of calcium lactate prepared by single-phase aragonite precipitated
calcium carbonate,” Res. J. Pharm. Biol. Chem. Sci., vol. 7, no. 1, pp. 1786–1794, 2016.
Y. Vebliza, W. Sjamsuridzal, A. Oetari, I. Santoso, and I. G. Roosheroe, “Re-identification of five strains of
Rhizopus arrhizus from tempeh based on ITS regions of rDNA sequence data,” AIP Conf. Proc., vol. 2023, no.
October, 2018.
A. S. Duniaji, W. Wisaniyasa, N. N. Puspawati, and dan N. M. Indri H, “Isolation and Identification of Rhizopusoligosporus Local Isolate Derived from Several Inoculum Sources,” Int. J. Curr. Microbiol. Appl. Sci., vol. 8, no.
, pp. 1085–1098, 2019.
R. Y. Zheng, G. Q. Chen, H. Huang, and X. Y. Liu, “A monograph of Rhizopus,” Sydowia, vol. 59, no. 2, pp. 273–
, 2007.
H. Kaur, “Optimization of α-amylase and glucoamylase production in solid state fermentation of deoiled rice bran
(DRB) by Rhizopus oryzae,” Int. J. Pure Appl. Biosci., vol. 3, no. 6, pp. 249–256, 2015.
A. C. De Freitas, B. Escaramboni, A. F. A. Carvalho, V. M. G. De Lima, and P. De Oliva-Neto, “Production and
application of amylases of Rhizopus oryzae and Rhizopus microsporus var. oligosporus from industrial waste in
acquisition of glucose,” Chem. Pap., vol. 68, no. 4, pp. 442–450, 2014.
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