STUDI AWAL PENGARUH METODE EKSTRAKSI TERHADAP RENDEMEN DAN KADAR ASIATICOSIDE DARI CENTELLA ASIATICA (L) URB
DOI:
https://doi.org/10.17146/jsmi.2016.17.3.4193Keywords:
Ekstraksi CO2 superkritik, Asiaticoside, Rendemen, HPLCAbstract
STUDI AWAL PENGARUH METODE EKSTRAKSI TERHADAP RENDEMEN DAN KADAR ASIATICOSIDE DARI CENTELLA ASIATICA (L) URB. Proses ekstraksi Centella asiatica (L) Urb dengan metode maserasi, sonikasi, sokletasi dan CO2 superkritik telah dilakukan. Pengaruh proses ekstraksi terhadap rendemen dan kadar asiaticoside dari Centella asiatica (L) Urb (pegagan) telah dipelajari. Hasil dari perhitungan rendemen asiaticoside terlihat bahwa kandungan asiaticoside (% berat) dari proses ekstraksimaserasi, sonikasi, sokletasi dan CO2 superkritik berturut-turut sebagai berikut: 6,723%; 0,187%; 3.648%dan 9,24%. Rendemen asiaticoside paling tinggi diperoleh dari teknologi ekstraksi CO2 superkritik, karena teknologi ini dilakukan pada tekanan dan suhu tertentu sehingga kualitas hasil ekstraksi ditentukan oleh seberapa kritis penggunaan tekanan dan suhunya. Karena pada kondisi ini, selain mengubah densitas CO2, juga berpengaruh terhadap kelarutan dan selektivitas dari zat yang akan terekstrak. Semakin tinggi tekanan dan kelarutan, total hasil ekstraksi akan semakin tinggi. Untuk mengetahui adanya senyawa asiaticoside dalam Centella asiatica (L) Urb (pegagan) digunakan analisis HPLC. Dari hasil analisis kromatogram bahwa ada dua puncak yang terdeteksi, dan secara kualitatif senyawa asiaticoside yang diperoleh denganmenggunakan ekstraksi CO2 superkritik lebih tinggi kadarnya dibanding metode ekstraksi lainnya, karena teknologi proses ekstraksi CO2 superkritik memanfaatkan kekuatan pelarut dan sifat fisik dari komponenmurni atau campuran, sehinggamudah melakukan penetrasi ke dalam dinding material yang di ekstrak dan melarutkan komponen senyawa aktif secara selektif dengan kualitas produk tinggi dan tidak mengandung residu pelarut sehingga lebih murni.Downloads
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