STUDI ABSORPSI Fe DALAM TUBUH ANAK MENGGUNAKAN METODE PERBANDINGAN ISOTOP STABIL
DOI:
https://doi.org/10.17146/jsmi.2005.6.3.4836Keywords:
Perbandingan Isotop Stabil, Stable Isotope Ratio, Perunut, Tracer, Absorpsi, Absorption, ICP-MS, ETV, Polyatomic Ions, Ion Poliatom, Susu Sapi, Cow's Milk, Jus Apel, Apple JuiceAbstract
STUDI ABSORPSI Fe DALAM TUBUH ANAK MENGGUNAKAN METODE PERBANDINGAN ISOTOP. Isotop stabil adalah bahan yang sangat berguna dalam penelitian absorpsi dan metabolisme nutrien anorganik dalam tubuh manusia. Prinsip dasar studi dengan isotop ini adalah menambahkan isotop stabil yang kelimpahannya rendah setelah diperkaya kedalam makanan. Dalam studi ini isotop stabil besi : 57Fe dan 58Fe digunakan sebagai perunut untuk mempelajari absorpsi Fe dalam tubuh anak. Perunut diberikan bersama susu sapi dan bersama jus apel. Perbandingan isotop Fe ditentukan menggunakan. ICP-MS (inductively coupled plasma-mass spectrometer) yang dilengkapi dengan ETV (elctrothermal vaporization) untuk pemasukan sampel. Pemasukan sampel menggunakan ETV mereduksi gangguan ion-ion poliatom, terutama gangguan terhadap 57Fe dari ion poliatom (40Ar16O1H). Diperoleh hasil bahwa absorpsi Fe oleh anak secara nyata lebih besar (13,8 + 3,8 %) bila diberikan bersama-sama jus apel dari pada bila diberikan bersama-sama susu sapi (5,8 + 1,3 %).
ABSTRACT
THE STABLE ISOTOP RATIOS METHOD FOR THE STUDY OF Fe ABSORPTION IN INFANTS. Stable isotopes are valuable tool for research on inorganic nutrient absorption and metabolism in human. The basic principle of an isotope study is to trace a meal or diet with the stable isotopes of enriched low natural abundance. In this study stable isotopes of iron (Fe) : 57Fe and 58Fe were used as tracer to study the Fe absorption in infants. The tracer given with a cow’s milk and an apple juice. Iron-isotope ratios were determined using ICP-MS (inductively coupled plasma-mass spectrometer) equipped with sample introduction by ETV (electrothermal vaporization). Sample introduction by ETV significantly reduces the levels of certain polyatomic ions, in particular those associated with the interference of 57Fe (40Ar16O1H). The result showed that Fe absorption by infants was significantly greater (13.8 + 3.8 %) when given with apple juice than that with cow’s milk (5.8 + 1.3 %).
Downloads
References
FRED MELLON, BRITTAMARIE SANDSTROM, Stable Isotopes in Human Nutrition Inorganic Nutrien Metabolism, Academic Press, (1996)
EHRENKRANZ, R. A., ACKERMAN, B. A.,NELLI, C. M., JANGHORBANI, Determination with Stable Isotopes of the Dietary Bioavailbility of Zinc in Premature Infants, Am. J. Clin. Nutr., 40 (72) (1984)
TING, BTG., KASPER, L.J., YOUNG, V.R., JANGHORBANI : Copper Absorption in Healty Young Men, Study with Stable Isotope 65Cu and Neutron Activation Analysis, Nutr. Res. 4 (757) (1984)
EHRENKRANZ, R.A., NELLI, C.M., JANGHORBANI : Absorption of Calcium in Premature Infants as Measured with a Stable Isotope 46Ca Extrinsic Tag, Pediatr. Res. 19 (178) (1985)
JANGHORBANI, TING, B.T.G., YOUNG, J. Nutr., 110 (2190) (1990)
CANTONE, M.C., MOHLO, N., PIROLA, L., GAMBARINI, G., HANSEN, C., ROTH, P., WERNER, E., Med. Phys., (1988)
WHITTAKER, P.G., LIND, T., WILLIAMS, J.G., GRAY,A.L., Analyst, 114 (675) (1989)
LONNERDAL, B., Iron Intake and Requirement : Nutritional Needs of the Six to Twelve Month Old
Infant, Carnation Nutrition Education Series, 2, (1991) 199-211
OSKI, F.A., Iron Deficiency in Infantcy and Childhood, J. Med. 329, (1993) 190-193
IDJRADINATA, P., POLLITT, E. Reversal of Developmental Delays in Iron-Deficient Anaemic Infants Treated with Iron, Lancet, 341 (14) (1993)
Expert Scientific Working Group, Summary of a Report on Assessment of the Nutritional Status of the United States Population, Am. J. Clin. Nutr. 42, (1985) 1318 – 1330
International Nutritional Anemia Consultative Group The Effects of Cereal and Legumes on Iron Availbility, Washington, DC., The Nutrition, (1982)
S. J FAIRWEATHER-TAIT, Iron Deficiency in Infancy; Easy to Prevent – or Is It ?, European Journal of Clinical Nutrition, 46 (4) (1992) S9-S14
HEINRICH, H. C., GABBE, E. E., WHANG, D. H., BENDER– GOTZE,C.SHAFER, K.H.,Ferrousand Hemoglobin 59Fe Absorption from Supplemented Cow Milk in Infants with Normal and Depleted Iron Stores, Z. Kinderheilkd 120 (1985) 251-258
HELBERG, L., Bioavailability of Iron in Man, Ann. Rev. Nutr., 1 (1991) 123 – 148
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
