CORRELATION DIVERSITY OF BACTERIA TO SECONDARY META BOLITES Nephthea spp. from SERIBU ISLANDS NATIONAL PARKS WATERS
Main Article Content
Abstract
Bioactive compounds from biota on coral reef is potentially of plasma nutfah that has high economic value. Their existence was influenced by the microbes which associated with them and the environmental conditions. The aim of the research was to analyze the correlation between the diversity of bacteria and secondary metabolites. Nephthea spp. collected from Seribu Islands National Parks (TNKpS). The analysis of bacteria diversity performed by Terminal Restriction Fragment Length Polymorphism (T-RFLP) and secondary metabolites used High Performance Liquid Chromatography (HPLC). The results of study showed that the range of diversity index (H), richness (R), and evenness index (E) of bacteria were 1,47–0,88; 13–3; 0,84–0,43. And for the secondary metabolites, the range were 2,21–1,12; 20-5; 0,95–0,63. Further study showed that there was a significant correlation (R =0.96 at p< 0.01) between bacteria and secondary metabolites group richness. The area which high diversity of bacteria and secondary metabolites were within central and southern TNKpS waters. This results were in line withthe best water quality in these areas.
Article Details
References
Hoover, C.A., M. Slattery, N.M. Targett, and A.G.
Marsh. 2008, Transcriptome and Metabolite
Responses to Predation in a South Pacific Soft
Coral, Biology Bulletin, 214: 319–328.
Widjhati R., A. Supriyono, dan Subintoro. 2004.
Pengembangan Senyawa Bioaktif dari Biota
Laut. Makalah Forum Bioteknologi Kelautan
dan Perikanan. Jakarta: 25 Maret 2004. Pusat
Riset Pengolahan Produk dan Sosial Ekonomi
Kelautan dan Perikanan, Depertemen Kelautan
dan Perikanan.
Britschgi, T. B. and S. J. Giovannoni. 1991.
Phylogenetic analysis of a natural marine bac
terioplankton population by rRNA gene cloning
and sequencing. Appl Environ Microbiol, 57:
1.707–1.713.
Urakawa, H., K.K. Tsukamoto, and K. Ohwada.
1999. Microbial diversity in marine sediments
from Sagami Bay and Tokyo Bay, Japan,
as determined by 16S rRNA gene analysis.
Microbiology, 145: 3.305–3.315.
Hentschel, U., K. M. Usher, and M. W. Taylor. 2006.
Marine sponges as microbial fermenters. FEMS
Microbiol Ecol. 55: 167–177.
Taylor, M. W., R. Radax, D. Steger, and M. Wagner.
2007. Sponge-associated microorganisms:
evolution, ecology and biotechnological
potential. Microbiol Mol Biol Rev, 71: 295–347.
Harder, T. et al. 2003. A distinctive epibiotic bacterial
community on the soft coral Dendronephthya
sp. and antibacterial activity of coral tissue
extracts suggest a chemical mechanism against
bacterial epibiosis. FEMS Microbiology Eco
logy, 43: 337–347.
Haygood, M.G., E.W. Schmidt, S.K. Davidson, and
D.J. Faulkner. 1999. Microbial Symbionts of
Marine Invetebrates: Opportunities for Micro
bial Biotecnology. Molecular Microbiology and
Biotechnology, 1(1):33–43.
Pringault, O., R. Duran, S. Jacquet, and J.P. Torreton.
2007. Temporal Variations of Microbial Activ
ity and Diversity in Marine Tropical Sediments
(New Caledonia Lagoon). Microb Ecol.
Pabel, C.T. et al. 2003. Antimicrobial Activites and
Matrix-Assisted Laser Desorption/Ionization
Mass Spectrometry of Bacullus Isolates from
the Marine Sponge Aplysina aerophoba.
Marine Biotechnology, 5: 424–434.
Januar, H.I. et al. 2010, Cytotoxic Cembranes
from Indonesia Specimens of the Soft Coral
Nephthea spp., Marine Drugs, 8: 2.142–2.152.
Chasanah, E. et al. 2010. Pengkajian Efek Perubahan
Iklim dan Stresor Antropogenik pada Senyawa
Bioaktif Ekologis Biota Invetebrata Terumbu
Karang di Lingkungan CTI. Laporan Teknis
Balai Besar Riset Pengolahan Produk dan
Bioteknologi Kelautan dan Perikanan 2010.
Jakarta.
Januar, H.I. et al. 2009, A Preliminary Study, Fine
Chemicals from Nephthea and Sarcophyton in
a Local Pressure from Environmental Stressors
at Seribu Islands Indonesia, Proceeding Paper
at Biotechnology Session -World Ocean Confer
ence, Manado 11–15 Mei 2009.
Holtrof, G. W. 2004. Jakarta Jabodetabek: Peta
Jalan dan Index. Edisi 14. PT Djambatan,
Indonesia.
Zhou, J., A. M. Bruns, and J.M. Tiedje. 1996. DNA
Recovery from Soils of Diverse Composition.
Applied and Environmental Microbiology, 62
(2): 316–322.
Allen, B., M. Kon, and B.Y. Yaneer. 2009. A new
phylogenetic diversity measure generalizing
the shannon index and its application to phyl
lostomid bats. The American Naturalist, 174(2)
Felske, A. et al.1997. Ribosome analysis reveals
prominent activity of an uncultured member
of the class Actinobacteria in grassland soils.
Microbiology. 143:2.983–2.989.
Isenbarger, T.A. et al. 2008. Miniprimer PCR, a new
lens for viewing the microbial world. Applied
and Environmental Microbiology: 840–849.
Moran, A.C. et al. 2008. Changes in Bacterial
Community Structure Associated with Coastal
Copper Enrichment. Enviromental Toxicology
and Chemistry, 27(11): 2.239–2.245.
Santosa, Y., E.P. Ramadhan, dan D.A. Rahman. 2008.
Studi Keragaman Mamalia pada Beberapa Tipe
Habitat di Stasiun Penelitian Pondok Ambung
Taman Nasional Tanjung Putting Kalimantan
Tengah. Media Konservasi, 13(3): 1–7.
Januar, H.I., T. Wikanta, dan E. Hastarini. 2005,
Analisis Spektrometri UV-Vis dan FT-IR
terhadap Gugus Fungsi Ekstrak Metanol
Rumput Laut Ulva fasciata Segar dan Kering,
Jurnal Penelitian Perikanan Indonesia Badan
Riset Kelautan dan Perikanan, 11(4): 1–9.
