SPERM LONGEVITY AND RECOVERY RATE OF BELGIAN BLUE CATTLE IN DIFFERENT EXTENDER
Main Article Content
Abstract
Frozen semen from Belgian Blue cattle is in high demand among farmers, as evidenced by the increasing interest in this breed's semen. However, the freezing process reduces sperm motility and viability. This study aimed to assess the viability of sperm post-freezing and the sperm recovery rate (RR) in Belgian Blue cattle using different extenders. Semen was collected, evaluated, and diluted in four extenders: three commercially available ones—BoviFree®, AndroMed®,, and Steridyl®,—and a homemade extender, Tris Egg Yolk (TEY). Longevity and RR tests were conducted 24 h after freezing. Progressive motility was assessed using a portable computer-assisted sperm analysis (CASA) device (Androscope Minitube, Germany). The results indicated that sperm motility was higher in the BoviFree®, and AndroMed®, extenders after 3 h compared to the other extenders (p <0.05). After 4 h, motility remained higher in the BoviFree®, extender than in TEY. The RR values showed no significant differences among the extenders (p< 0.05). The study concluded that sperm longevity in Belgian Blue cattle was optimal in the BoviFree®, extender, with consistently good sperm RR values across all extenders.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
a). Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Attribution-NonCommercial-ShareAlike 4.0 International that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b). Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
References
Aditya NR, Dudi D, Arifin J (2024) Kajian Kraniometri Persilangan Sapi Belgian Blue dengan Sapi Peranakan Ongole di Jawa Barat. J Produksi Ternak Terap 5:1–10. https://doi.org/10.24198/jptt.v5i1.42413
Amal AS, Arifiantini RI, Setiadi MA, Said S (2019) Characteristics of the post-thawed Balinese bull semen extended in three different extenders and equilibration times. J Indones Trop Anim Agric 44:135. https://doi.org/10.14710/jitaa.44.2.135-145
Ananda A, Gusdinal H, Ramadhan R, Abimanyu AA, Ningsih WH, Jaswandi J (2025) Impacts of Cryopreservation on Semen Quality and Sperm Protein Profiles of Pesisir Bulls. Trop Anim Sci J 48:189–198. https://doi.org/10.5398/tasj.2025.48.3.189
Arif AA, Maulana T, Kaiin EM, Purwantara B, Arifiantini RI (2022) The Quality of Frozen Semen of Limousin Bull in Various Semen Diluents. Trop Anim Sci J 45:284–290. https://doi.org/10.5398/tasj.2022.45.3.284
Arifiantini I, Yusuf TL, Graha N (2005) Longivitas dan Recovery Rate Pasca Thawing Semen Beku Sapi Fresian Holstein Menggunakan Bahan Pengencer yang Berbeda. Bul Peternak 29:53. https://doi.org/10.21059/buletinpeternak.v29i2.1163
Arifiantini RI, Karja NWK, Susilawati T, Said S MH (2024) Manual Produksi Semen beku Sapi di Indonesia. IPB Press, Bogor
Baharun A, Arifiantini RI, Yusuf TL (2017) Freezing Capability Of Pasundan Bull Sperm Using Tris-Egg Yolk, Tris-Soy, and Andromed® Diluents. J Vet Sci 11:45–49. https://doi.org/10.21157/j.ked.hewan.v11i1.5810
Badan Standarisasi Nasional (BSN) (2024) SNI 4869-1 Semen Beku Bagian 1: Sapi
Bustani GS, Baiee FH (2021) Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders. Vet World 1220–1233. https://doi.org/10.14202/vetworld.2021.1220-1233
Garner DL, Hafez ESE (2000) Chapter 7 Spermatozoa and Seminal Plasma: Reproduction in farm Animals 7 th Edition. Williams & Wilkins, Baltimore
Hanifah NF, Ratnani H, Purnama MTE, Restiadi TI, Agustono B, Prastiya RA (2020) Pengaruh Konsentrasi Gliserol dalam Pengencer Tris Terhadap Kualitas Spermatozoa Kambing Sapera Before Freezing. J Med Vet 3:154. https://doi.org/10.20473/jmv.vol3.iss2.2020.154-159
Indriastuti R, Ulum MF, Arifiantini RI, Memili E, Purwantara B (2020) Relationship among Body Weight, Scrotal Circumference and Sperm Quantity of Bali Bulls in Baturiti Artificial Insemination Center. IOP Conf Ser Earth Environ Sci 478:012004. https://doi.org/10.1088/1755-1315/478/1/012004
Larasati MD, Lestari SW, Hestiantoro A, Pangestu M (2022) Can Cryoprotectant’s modification in Spermatozoa Cryopreservation be an Alternative to Improve Embryo Quality? A Review. Int J Technol 13:1755. https://doi.org/10.14716/ijtech.v13i8.6129
Matahine T, Burhanuddin, Marawali A (2014) Efektivitas Air Buah Lontar dalam Mempertahankan Motilitas, Viabilitas dan Daya Tahan Hidup Spermatozoa Sapi Bali. J Vet 15:263–273
Miguel-Jimenez S, Rivera del Alamo MM, Álvarez-Rodríguez M, Hidalgo CO, Peña AI, Muiño R, Rodríguez-Gil JE, Mogas T (2020) In vitro assessment of egg yolk-, soya bean lecithin- and liposome-based extenders for cryopreservation of dairy bull semen. Anim Reprod Sci 215:106315. https://doi.org/10.1016/j.anireprosci.2020.106315
Mokhtassi-Bidgoli A, Sharafi M, Benson JD (2023) Optimizing Bull Semen Cryopreservation Media Using Multivariate Statistics Approaches. Animals 13:1077. https://doi.org/10.3390/ani13061077
Riwu RMJ, Arifiantini RI, Karja NWK (2023) Comparison of Different Lecithin Diluents for Cryopreservation of Toraya Buffalo Semen. Trop Anim Sci J 46:396–402. https://doi.org/10.5398/tasj.2023.46.4.396
Sharafi M, Borghei-Rad SM, Hezavehei M, Shahverdi A, Benson JD (2022) Cryopreservation of Semen in Domestic Animals: A Review of Current Challenges, Applications, and Prospective Strategies. Animals 12:3271. https://doi.org/10.3390/ani12233271
Solís JM, Sevilla F, Silvestre MA, Araya-Zúñiga I, Roldan ERS, Saborío-Montero A, Valverde A (2024) Effect of Thawing Procedure and Thermo-Resistance Test on Sperm Motility and Kinematics Patterns in Two Bovine Breeds. Animals 14:2768. https://doi.org/10.3390/ani14192768
Sukirman I, Sukmawati E, Rasad SD, Solihati N (2020) The Influence of Breed and Type of Extender on the Quality of Bull Semen. Anim Prod 21:64. https://doi.org/10.20884/1.jap.2019.21.2.641
Tahar MA, Komariah K, Nuraini H, Maulana T, Gunawan M, Arifiantini RI (2022) Assessment of sperm acrosome status, malondialdehyde and aspartate aminotransferase enzyme concentration of frozen semen from Limousin and Simmental bulls in different commercial diluents. J Ilmu Ternak dan Vet 27:122–129. https://doi.org/10.14334/jitv.v27i3.3049
Ugur MR, Amal AS, Evans HC, Gilmore AA, Hitit M, Arifiantini RI, Purwantara B, Kaya A, Memili E (2019) Advances in Cryopreservation of Bull Sperm. Front Vet Sci 6. https://doi.org/10.3389/fvets.2019.00268
Xu Z, Yan Q, Zhang K, Lei Y, Zhou C, Ren T, Gao N, Wen F, Li X (2025) Mitochondrial Regulation of Spermatozoa Function: Metabolism, Oxidative Stress and Therapeutic Insights. Animals 15:2246. https://doi.org/10.3390/ani15152246
