KARAKTERISTIK DAN SIFAT KINETIKA ENZIM KITINASE ASAL JAMUR ENTOMOPATOGEN Beauveria bassiana
Main Article Content
Abstract
Beauveria bassiana merupakan salah satu jamur entomopatogen yang memproduksi kitinase saat menginfeksi inangnya. Enzim kitinase saat ini banyak digunakan sebagai agen biokontrol karena dapat mendegradasi kitin menjadi produk yang ramah lingkungan. Penelitian ini bertujuan untuk mengkarakterisasi dan menguji kinetika enzim kitinase asal jamur B. bassiana. Metode yang digunakan dalam karakterisasi ini mencakup penentuan pH dan suhu optimum, kestabilan enzim pada suhu optimumnya, dan uji kinetika enzim yang mencakup penentuan nilai Km dan Vmaks dengan persamaan Lineweaver-Burk. Hasil penelitian karakterisasi menunjukkan bahwa enzim kitinase B. bassiana mempunyai pH dan suhu optimum masing-masing 5 dan 40ºC. Enzim ini stabil sampai pada 90 menit inkubasi pada suhu 40ºC. Nilai Km diperoleh 0,181 mg/L dan Vmaks sebesar 0,022 mg/L.detik. Nilai Km lebih tinggi daripada Vmaks, yang artinya afinitas enzim terhadap substrat rendah sehingga membutuhkan konsentrasi substrat yang tinggi untuk meningkatkan kecepatan reaksi, maka dapat disimpulkan bahwa aktivitas kitinase dari B. bassiana masih tergolong rendah.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
a). Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Attribution-NonCommercial-ShareAlike 4.0 International that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b). Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
References
de Carolina Sanchez-Perez L, Barranco-Florido JE, Rodriguez-Navarro S, Cervantes-Mayagoitia JF, Ramos-Lopez MA (2014) Enzymes of entomopathogenic fungi, advances and insights. Adv Enzyme Res 2: 65-76. Doi: 10.4236/aer.2014.22007
Haedar N, Natsir H, Fahruddin, Aryanti W (2017) Produksi dan karakterisasi enzim kitinase dari bakteri kitinolitik asal kerang Anadara granosa. J Ilmu Alam Ling 8: 14-21.
Jung WJ, Park RD (2014) Bioproduction of chitooligosaccharides: Present and perspectives. Mar Drugs 12:5328-5356. Doi: 10.3390/md12115328
Karthik N, Akanksha K, Binod P, Pandey A (2014) Production, purification and properties of fungal chitinase – A Review. Indian J Exp Biol 52: 1025-1035.
Kulu IP, Abadi AL, Afandhi A, Nooraidawati (2015) Morphological and molecular identification of Beauveria bassiana as entomopathogen agent from Central Kalimantan peatland, Indonesia. Int J ChemTech Res 8:2079-2084.
Ma GZ, Gao HL, Zhang YH, Li SD, Xie BY, Wu SJ (2012) Purification and characterization of chitinase from Gliocladium catenulatum strain HL-1-1. Afr J Microbiol Res 6: 4377–4383. Doi: 10.5897/AJMR12.605
Nguyen HQ, Quyen DT, Nguyen SLT, Vu VH (2015) An extracellular antifungal chitinase from Lecanicillium lecanii: purification, properties, and application in biocontrol against plant pathogenic fungi. Turk J Biol 39:6-14. Doi: 10.3906/biy-1402-28
Noviendri D, Fawzya YN, Chasanah E (2008) Karakteristik dan sifat kinetika enzim kitinase dari isolat bakteri T5a1 asal terasi. J Pascapanen Bioteknol Kelautan dan Perikanan 3:123-129.
Pratiwi RS, Susanto TE, Wardani YAK, Sutrisno A (2015) Enzim kitinase dan aplikasi di bidang industri: Kajian Pustaka. J Pangan Agroindustri 3: 878-887.
Purkan P, Baktir A, Sayyidah AR (2016) Produksi Enzim kitinase dari Aspergillus niger menggunakan limbah cangkang rajungan sebagai induser. J Kimia Riset 1: 34 – 41. Doi: 10.20473/jkr.v1i1.2440
Rahmawati H, Purnomo AJ, Umniyatie S, Pramiadi D, Sari N (2016) Identification and characterization of chitinase enzyme producing bacteria from bat guano and its potential to inhibit the growth of fungus Colletotrichum sp. cause anthracnose on the chili by in vitro. Int J Adv Agricultural & Environmental Engg 3: 249-254. Doi: 10.15242/IJAAEE.U0516208
Suryadi Y, Priyatno TP, Susilowati DN, Samudra IM, Yudhistira N, Purwakusumah ED (2013) Isolasi dan karakterisasi kitinase asal Bacillus cereus 11 UJ. J Biol Indon 9: 51-62. Doi: 10.14203/jbi.v9i1.146
Ueda M, Arai M (1992) Purification and some properties of chitinase from Aeromonas sp. No. 10S-24. Biosci Biotech Biochem 56: 460-464. Doi: 10.1271/bbb.56.460
Wang J, Zhang J, Song F, Gui T, Xiang J (2015) Purification and characterization of chitinases from ridgetail white prawn Exopalaemon carinicauda. Molecules 20:1955-1967. Doi: 10.3390/molecules20021955
Zhang J, Cai J, Wu K, Jin S, Pan R, Fan M (2004) Production and properties of chitinase from Beauveria bassiana Bb174 in solid state fermentation. Chin J Appl Ecol 5: 863-866.