MICROPROPAGATION OF POTATO (Solanum tuberosum L.) cv. GRANOLA IN LIQUID MEDIUM USING AERATION SYSTEM FOR G0 SEED PRODUCTION
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Abstract
Disease-free potato seeds of high quality can be obtained via in vitro culture. The use of liquid medium during in vitro cultures might boost the number of plantlets produced, however, the problem of hyperhydricity in plantlets was often encountered. This study aimed to investigate effects of different sucrose concentrations and application of aeration system on micropropagation of potato cv. Granola using liquid medium. Aseptic nodal explants with 3-4 nodes from established in vitro cultures were subjected to MS liquid medium with a factorial treatment of three sucrose concentrations (0, 7.5, 15 g. L-1) and two culture (with and without aeration). The results showed that MS medium with 7.5 g L-1 sucrose was the best medium to produce the highest number of shoots and nodes. Furthermore, it was found that application of aeration system in MS liquid culture decreased plantlet hyperhydricity and increased the number of shoots, number nodes, plantlet height, as well as improved plantlet morphology and vigor. Application of the aeration system in liquid medium produced 200-230 new potato plants per bioreactor in the acclimatization stage and an average of 2773.5 G0 mini tubers.
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References
Aighewi BA, Asiedu R, Maroya N, Balogun M (2015) Improved propagation methods to raise the productivity of yam (Discorea rotundata Poir.). Food Secur 7:823–834. doi: 10.1007/s12571-015-0481-6
Akhiriana E, Samanhudi, Yunus A (2019) Coconut water and IAA effect on the in vitro growth of Tribulus terrestris L. Acta Univ Agric Silvic Mendelianae Brun 67:9-18. doi: 10.11118/actaun201967010009
Bamberg JB, Martin MW, Abad J, Jenderek MM, Tanner J, Donnelly DJ, Nassar AMK, Veilleux RE, Novy RG (2016) In vitro technology at the US Potato Genebank. In vitro Cell Dev Biol Plant 52:213-225. Biol-Plant. doi: 10.1007/s11627-016-9753-x
Bhatia S, Sharma K (2015) Technical glitches in micropropagation. In: Modern Application of Plant Biotechnology in Pharmaceutical Sciences. Chapter 13 Pp. 393–404. Academic Press Boston. doi: 10.1016/b978-0-12-802221-4.00013-3
Borna RS, Hoque MI, Sarker RH (2019) In vitro microtuber induction and regeneration of plantlets from microtuber discs of cultivated potato (Solanum tuberosum L.). Plant Tissue Cult Biotechnol 29:63-72. doi: 10.3329/ptcb.v29I1.41979
BPTP (2014) Mengenal beberapa varietas kentang dan mandaatnya. Lembar Informasi Pertanian No 04/DH/2914. Badan Pengkajian Teknologi Pertanian, Sumatera Selatan
Caliscan ME, Yavuz Z, Yagiz AK, Demirel U, Caliscan S (2021) Comparison of aeroponics and convensional potato mini tuber production system at different plant densities.Potato Research 64:41-53. doi: 10.1007/s11540-020-09463-z
Charkowski A, Sharma K, Parker ML, Secor GA, Elphinstone J (2020) Bacterial diseases of potato. In: Campos H, Ortiz O (Eds). The Potato Crop. Springer Cham. doi: 10.1007/978-3-030-28683-5-10
Cheng L, Wang D, Wang Y, Xue H, Zhang F (2020) An integrative overview of physiological and proteomic changes of cytokinin-induced potato (Solanum tuberosum L.) tuber development in vitro. Physiol Plant 168:675-693. doi: 10.1111/ppl.13014
Cournac L, Dimon B, Carrier P, Lohou A, Chagvardieff P (1991) Growth and photosynthetic characteristics of Solanum tuberosum plantlets cultivated in vitro in different conditions of aeration, sucrose supply, and CO2 enrichment. Plant Physiol 97:112–117. doi: 10.1104/pp.97.1.112
De L Tapia M, Arbizu C, Beraun F, Lorenzo J, Escalona M (2018) Pre-basic seed potato (Solanum tuberosum L.) production using temporary immersion bioreactors. Peruvian J Agron 2:9-14. doi: 10.21704/pja.v2i1.1127
Dessoky ES, Attia AO, Ismail IA, El-Hallous EI (2016) In vitro propagation of potato under different hormonal combinations. Int J Adv Res 4:684-689
different concentrations of coconut water in in-vitro. IOP Conf Series: Earth and Environmental Science 711:012021. doi: 10.1088/1755-1315/711/1/012021
Forbes GA, Charkowski A, Andrade-Piedra J, Parker ML, Schulte-Geldermann E (2020) Potato seed systems. In: Campos H, Ortiz O (Eds). The Potato Crop. Springer Cham. doi: 10.1007/978-3-030-28683-5_12
Gong H, Igiraneza C, Dusengemungu L (2019) Major in vitro techniques for potato virus elimination and post eradication detection methods. A Review. Am J Potato Res 96:379-389. doi: 10.1007/s12230-019-09720-z
Hajare ST, Chauhan NM, Kassa G (2021) Effect of growth regulators on in vitro micropropagation of potato (Solanum tuberosum L.) Gudiene and Belete varieties from Ethiopia. Sci World J 2021:5928769. doi: 10.1155/2021/5928769
Hassankhah A, Vahdati K, Lotfi M, Mirmasoumi M, Preece J, Assareh MH (2014) Effect of ventilation and sucrose concentrations on the growth and plantlet anatomy of micropropagated persian walnut plants. Int J Hortic Sci Tech 1:111–120. doi: 10.22059/IJHST.2014.52781
Hossain MS, Hossain MM, Haque MM, Haque MM, Sarkar MD (2017) Varietal evaluation of potato microtuber and plantlet in seed tuber production. Int J Agron 2017:7520297. doi: 10.1155/2017/7520297
Karjadi AK (2014) Pengaruh penyemprotan GA3 dan asal tanaman induk dalam memperpanjang masa juvenile tanaman kentang. Agrin 18:97–106. doi: 10.20884/1.agrin.2014.18.2.216
Karyanti K, Kristianto YG, Khairiyah H, Novita L, Sukarnih T, Rudiyana Y, Sofia DY (2018) Pengaruh wadah kultur dan konsentrasi sumber karbon pada perbanyakan kentang atlantik secara In vitro. J Bioteknol Biosain Indones 5:177-187. doi: 10.29122/jbbi.v5i2.3012
Kazemiani S, Motallebi-Azar AR, Panahandeh J, Mokhtarzadeh S, Ozdemir FA (2018) Shoot proliferation from potato (Solanum tuberosum cv. Agria) under different concentration of MS include vitamin and BAP medium. Progr Nutr 20:160-166. doi: 10.23751/pm.v20i1-S.6686
Kim S, Da K, Mei C (2012) An efficient system for high-quality large-scale micropropagation of Miscanthus x giganteus plants. In Vitro Cell Dev Biol Plant 48:613–619. doi: 10.1007/s11627-012-9472-x
Kuncoro CBD, Sutandi T, Adristi C, Kuan YD (2021) Aeroponics root chamber temperature conditioning design for smart mini-tuber potato seed cultivation. Sustainability 13:5140. doi: 10.3390/su13095140
Mamiya K, Tanabe K, Onishi N (2020) Production of potato (Solanum tuberosum, L.) microtubers using plastic culture bags. Plant Biotechnol 37:233-238. doi: 10.5511/plantbiotechnology.20.0312a
Mbiyu M, Muthoni J, Kabira J, Muchira C, Pwaipwai P, Ngaruiya J, Onditi J, Otieno S (2012) Comparing liquid and solid media on the growth of plantlets from three Kenyan potato cultivars. Am J Exp Agric 2:81-89. doi: 10.9734/AJEA/2012/715
Mohamed MAH, Alsadon AA (2010) Influence of ventilation and sucrose on growth and leaf anatomy of micropropagated potato plantlets. Sci Hortic 123:295–300. doi: 10.1016/j.scienta.2009.09.014
Mohapatra PP, Batra VK (2017) Tissue culture of potato (Solanum tuberosum L.): A review. Int J Curr.Microbiol App Sci 6:489-495. doi: 10.20546/ijcmas.2017.604.058
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473-497. doi: 10.1111/j.1399-3054.1962.tb08052.x
Ni’mah F, Ratnasari E, Budipramana LS (2012) Pengaruh pemberian berbagai kombinasi konsentrasi sukrosa dan kinetin terhadap induksi umbi mikro kentang (Solanum tuberosum L.) kultivar Granola kembang secara in vitro. LenteraBio 1:41–48
Oraby H, Lachance A, Desjardins Y (2015) Low nutrient solution temperature and the application of stress treatments increase potato mini-tubers production in an aeroponic system. Am J Potato Res 92:387–397. doi: 10.1007/s12230-015-9444-x
Oves EV, Zhevora SV, Gaitona NA, Boyko NA, Fenina NA, Shishkina OA (2021) Assessment of potato in vitro morphogenesis. IOP Conf Series: Earth and Environmental Science 659:012093. doi: 10.1088/1755-1315/659/1/012093
Park J, Park Y, Thi L, Soundararajan, Jeong B (2018) Effect of sucrose concentration, photosynthetic photon flux density, and CO2 concentration on growth and development of micropropagated Mountain ash. Propag Ornam Plants 18:58–63
Potato tuber (Solanum tuberosum L.) formation due to the application of
Prando MAS, Chiavazza P, Faggio A, Contessa C (2014) Effect of coconut water and growth regulator supplements on in vitro propagation of Corylus avellana L. Sci Hortic 171:91-94. doi: 10.1016/j.scienta.2014.03.052
Qureshi MA, Gul Z, Ali S, Khan AR (2014) Comparative growth response of potato plantlets developed on liquid vs solidified (MS) medium using tissue culture technology. Int J Recent Sci Res 5:736–9
Rai R, Diengdoh IC, Srivastava AK, Bag TK (2012) Efficiency of different nodal segments for potato micro-propagation. Environ Ecol 30:594–597
Rai SP, Wiendi NMA, Krisantini (2015) Optimasi produksi bibit tanaman kentang (Solanum tuberosum) kultivar Granola dengan teknik fotoautotrofik. Bul Agrohorti 3:28–38. doi: 10.29244/agrob.v3i1.14822
Rao SS, Najam R (2016) Coconut water of different maturity stages ameliorates inflammatory processes in model in inflammation. J Intercult Ethnopharmacol 5:244-249. doi: 10.5455/jice.20160402120142
Saji KV, Sujatha M (1998) Embryogenesis and plant regeneration in anther culture of sunflower (Helianthus annuus L.). Euphytica 103:1–7. doi: 10.1023/1a:1018318625718
Salem J, Hassanein AM (2017) In vitro propagation, microtuberization, and molekuler characterization of three potato cultivars. Biol Plant 61:427-437. doi: 10.1007/s10535-017-0715-x
Sembiring R, Hayati M, Kesumawti E (2021)
Sharma SK, Bryan GJ, Winfield MO, Millam S (2007) Stability of potato (Solanum tunerosum L.) plants regenerated via somatic embryo s, axillary bud proliferated shoots, microtubers and true potato seeds: a comparative phenotypic, cytogenetic, and molecular assessment. Planta 226:1449-1458. doi: 10.1007/s00425-007-0583-2
Singh RK, Buckseth T, Tiwari J, Sharma AK, Singh V, Kumar D, Venkataslam EP, Singh RK, Sdawarti MJ, Challam C, Chakrabarti SK (2019) Seed potato (Solanum tuberosum) production system in India: A chronological outlook. Indian J Agric Sci 89:578-587
Steffens B, Rasmussen A (2016) The physiology of adventitious roots. Plant Physiol 170:603–167. doi: 10.1104/pp.15.01360
Sugihono C, Hasbianto A (2014) Perkembangan penggunaan teknik kultur jaringan pada tanaman kentang (Solanum tuberosum L.). Pp. 435–443. Pros Seminar Nas Inov Teknologi Pertanian Spesifik Lokasi. 6-7 August 2014, Banjarbaru.
Tierno R, Carrasco A, Ritter E, de Galarreta JIR (2014) Differential growth response and minituber production of three potato cultivars under aeroponics and greenhouse bed culture. Am J Potato Research 91:346–353. doi: 10.1007/s12230-013-9354-8
Yaseen M, Ahmad T, Sablok G, Standardi A, Hafiz IA (2013) Review: Role of carbone sources for in vitro plant growth and development. Mol Biol Rep 40:2837–2849. doi: 10.1007/s11033-012-2299-z
Zhang Z, Wang QC, Spetz C, Blystad DR (2019) In vitro therapies for virus elimination of potato-valuable germplasm in Norway. Sci Hortic 249:7-14. doi: 10.1016/j.scienta.2019.01.027