KIDNEY MICROANATOMY OF WHITE RATS ON THE ADMINISTRATION OF ETHANOL EXTRACT OF Spatholobus littoralis STEM ACUTE DOSE
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Abstract
Tampala bajakah stem (Spatholobus littoralis Hassk) is empirically used by the Indonesian people to recover from disease and maintain health, but the use of tampala bajakah has not been tested for doses that are safe for consumption. The purpose of this study was to determine the type of damage caused by acute doses of bajakah tampala stem extract on white rat kidney microanatomy (Rattus norvegicus Berkenhout). This study used bajakah stem derived from Ambawang, extraction using ethanol solvent, and Wistar strain white rat (± 150g) test as a test animal. The method used a Complete Randomized Design with 4 treatments namely normal control using distilled water, treatment of doses of 300, 2000, and 5000 mg/kg BW of bajakah tampala stem extract. Each group was given 5 replicates. The extract was shown in a single dose orally and observations were made for 14 days. The results obtained that doses of 2000 and 5000 mg/kg BW caused damage to white rat kidney tissue in the form of microanatomy dilatation of tubules and loss of the brush border, and doses of 300, 2000, and 5000 mg/kg BW caused necrosis and haemorrhage. The most severe damage to kidney microanatomy is the dose of 2000 and 5000 mg/kg BW. The administration of acute doses of bajakah extract has the potential to cause damage to kidney tissue.
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References
Anzini, N., I. Kusharyanti, and S.N. Nurbae-ti. 2014. Uji Toksisitas Akut Fraksi Etil Asetat Batang dan Daun Pacar Air (Impatiens balsamina Linn) ter-hadap Tikus Putih Betina Galur Spra-gue Dawley, J Trop Pharm Chem, 2(4): 235-247.
Assiam, N., I. Setyawati, and S.K. Sudirga. 2014. Pengaruh Dosis dan Lama Per-lakuan Ekstrak Daun Kaliandra Merah (Calliandra calothyrsus Meissn.) ter-hadap Struktur Histologi Ginjal Mencit (Mus musculus L.), Jurnal Simbiosis, 2(2): 236-246.
Ayuchecaria, N., M.M.A. Saputera, and R. Niah. 2020. Penetapan Kadar Fenolik Total Ekstrak Batang Bajakah Tampa-la (Spatholobus littoralis Hassk) menggunakan UV-Visible, Jurnal In-san Farmasi Indonesia, 3(1): 132-141.
Badan Penelitian dan Pengembangan Kesehatan Kementerian Kesehatan RI. 2023. Riset Kesehatan Dasar, Ja-karta.
BPOM (Badan Pengawas Obat dan Ma-kanan). 2012. Pedoman Uji Toksisitas Nonklinis Secara In Vivo.
BPOM (Badan Pengawas Obat dan Ma-kanan). 2014. Pedoman Uji Toksisitas Nonklinis Secara In Vivo.
Caturizani, D.S. 2016. Uji Toksisitas Akut Ekstrak Etanol 96% Daun Pasir-Pasir (Ilex cymosa Blume) pada Tikus Betina Galur Wistar, Jurnal Maha-siswa Farmasi Fakultas Kodekteran UNTAN, 3(1).
Fahriyansyah, F., S. Isdadiyanto, S.M. Mardiati, and A.J. Sitasiswi. 2021. Gambaran Histologi Ren Tikus Putih (Rattus norvegicus L.) Hiperglikemia Setelah Pemberian Ekstrak Etanol Daun Mimba (Azadirachta indica A. Juss), Buletin Anatomi dan Fisiologi, 6(2): 193-202.
Gutierrez, E., J. Egido, A. Rubio-Navarro, I. Buendia, and L.M.B. Colio. 2012. Ox-idative Stress, Macrophage Infiltration and CD163 Expression are Determi-nants of Long Term Renal Outcome Acute Kidney Injury of IgA Nephropa-thy, Nephron Clin Pract, 121: 42-53.
Ichsan, M.Z., H. Febriani, and Syukriah. 2022. Efek Pemberian Ekstrak Etanol Rimpang Jeringau terhadap Gam-baran Morfohistologi Ginjal Tikus Pasca Induksi Natrium Nitrit, Klorofil, 6(2):1-13.
Ismail, A.E.T.M., A.M. Moustafa, and B.G. Abd E-Rhman. 2003. Microscopic Studies of The Effect of some Food Additives on The Kidney of Albino Rat, The Egyptian of Hospital Medi-cine, 12(1):12-27.
Kumar, V., R. Cotran, S.L. Robbins. 2012. Buku Ajar Patologi. Edisi 7. Jakarta: EGC.
Maulina. 2019. Uji Fitokimia Kadar Ekstrak Etanol Batang Bajakah Tampala (Spatholobus littoralis Hassk), Jurnal Ilmiah Manuntung, 5(2): 167-173.
Mayori, R., N. Marusin N, D.H. Tjong. 2013. Pengaruh Pemberian Rhodamin B terhadap Struktur Histologi Ginjal Mencit Putih (Mus musculus L.), Jurnal Biologi Universitas Andalas, 2(1):1-7.
McMurry, J., and R.C. Fay. 2004. McMurry Fay Chemistry 4th Edition. Belmont: Pearson Education International.
Muthmainnah, N., H.F. Trianto, and P.I. Bangsawan. 2015. Uji Toksisitas Akut Ekstral Etanol 70% Daun Karamunt-ing (Rhodomyrtus tomentosa (Aiton) Hassk) terhadap Gambaran His-topatologi Ginjal Tikus, Jurnal Cere-bellum, 1(4): 277-292.
Pringgoutomo, S. 2006. Buku Ajar Patologi 1 (Umum). Jakarta: Sagung Seto.
Rahman, F., B. Oktomalioputri, and M. Ir-ramah. 2020. Pengaruh Pemberian Ekstrak Daun Duwet (Syzigium cumini) terhadap Gambaran Histologi Ginjal Tikus (Rattus novergicus) yang Diintokdikasikan dengan Timbal Asetat, Jurnal Kesehatan Andalas, 9(1): 171-177.
Rinaldi, S.F., and B. Mujianto. 2017. Metod-ologi dan Statistik, Jakarta: Penerbit Pusdiknakes
Sacher, R.A., and R.A. McPherson. 2004. Tinjauan Klinis Hasil Pemeriksaan Laboratorium. Edisi 11, Jakarta: EGC.
Saputera, M.M.A., and N. Ayuchecaria. 2018. Uji Efektivitas Ekstrak Etanolik Btang Bajakah (Spatholobus littoralis Hassk) terhadap Waktu Penyem-buhan Luka, Journal of Chemical In-formation and Modeling, 53(9): 1689-1699.
Saputera, M.M.A., T.W.A. Marpaung., and N. Ayuchecaria. 2019. Konsentrasi Hambat Minimum (KHM) Kadar Ekstrak Etanol Batang Bajakah Tam-pala (Spatholobus littoralis Hassk) Terhadap Bakteri Escherichia coli me-lalui Metode Sumuran, Jurnal Ilmiah Manuntung, 5(2): 167-173.
Sari, W., S. Fitri., R. Ceriana., and Sunarti. 2017. Efek Toksik Pemberian Ekstrak Batang Sipatah-patah (Cissus quad-rangula Salisb.) terhadap Gambaran Histologi Hati dan Ginjal Mencit, Bi-oleuser, 1(1): 31-37.
Septiva, E.B., A.J. Sitasiswi, and S. Isdadi-yanto. 2019. Struktur Mikroanatomi Ginjal Mencit (Mus musculus L.) Betina Setelah Paparan Ekstrak
Etanol Daun Mimba (Azadirachta in-dica A. Juss), Jurnal Pro-Life, 6(2):180-190
Setiawan, A., Siswanto, Erwando, and Mu-htarudin. 2022. Pengaruh Suplemen-tasi Tepung Krokot (Portulaca oleraceae L.) dengan Taraf yang Ber-beda terhadap Kadar Total Total Pro-tein Plasma, Albumin dan Globulin Kambing Jawarandu (Capra aegagrus hircus), Jurnal Riset dan Inovasi Pe-ternakan, 6(2): 164-172.
Shafaei, H., A. Esmaeili, J.S. Rad, A. Delazar, and M. Behjati. 2012. Citrul-lus colocynthis as A Medicine or Poi-sonous Plant, Journal of Medicinal Plants Research, 6(35): 4922-2927.
Sinaga, F.A. 2016. Stres Oksidatif dan Sta-tus Antioksidan pada Aktivitas Fisik Maksimal, Generasi Kampus, 9(2): 176-189.
WHO (World Health Organization). (2018). Traditional Drugs.
Yusro, F., and Y. Mariani Y. 2021. Tradi-tional Medical Plants Used by the Community of Sri Wangi Village, Kapuas Hulu Regency, Jurnal Biologi Tropis, 21(3): 1030-1037.