EFFECT OF STORAGE TIME VARIATION OF ANDALIMAN FRUIT ON FREE RADICAL SCAVENGING AND PROLIFERATION INHIBITION ON MCF-7 CELLS

Authors

  • Idah Rosidah Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN)
  • Siska Andrina Kusumastuti Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN)
  • Grace Sophiwati Simanungkalit Faculty of Biotechnology, DEL Institute of Technology, Toba, North Sumatera, Indonesia
  • Maya Damayanti Rahayu Research Center for Pharmaceutical Ingredient and Traditional Medicine, National Research and Innovation Agency (BRIN)
  • Nuralih Nuralih Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), Bogor, Indonesia
  • Merry Meryam Martgrita Faculty of Biotechnology, DEL Institute of Technology, Toba, North Sumatera, Indonesia
  • Roga Florida Kembaren Faculty of Biotechnology, DEL Institute of Technology, Toba, North Sumatera, Indonesia
  • Chaidir Chaidir Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), Bogor, Indonesia
  • Rofiq Sunaryanto Research Centre for Food Technology and Process, National Research and Innovation Agency (BRIN), Yogyakarta, Indonesia
  • Agung Eru Wibowo Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), Bogor, Indonesia

DOI:

https://doi.org/10.55981/beritabiologi.2024.3334

Keywords:

Methanol extract of Andaliman fruit, storage time, radical scavenging, proliferation inhibition of MCF-7

Abstract

Andaliman (Zanthoxylum acanthopodium D.C) is a plant widely used by the Batak people in North Sumatera. Several studies demonstrated that Andaliman fruit exhibits antioxidant activities and cytotoxic effects on cancer cells. Quality of Andaliman fruit could affected by post-harvest process including storage time. This research aims to analyze the compound, antioxidant activity and proliferation inhibition on MCF-7 cells of Andaliman fruit extracts (AFE) stored at varying storage time. A series of fresh 250 g Andaliman fruit sealed in a closed container and stored in the oven at 30ºC. After storage at 0, 24, 48, 72, 96, 120 and 144 hours, Andaliman fruit macerated using methanol and obtained successive extract yields of 4.12; 3.70; 12.79; 8.17; 8.15; 3.7 and 2.21. These extracts were analyzed for total phenolic, total flavonoids, free radical scavenging activity and proliferation inhibition on MCF-7 cells. The chemical compound analysis of AFE was performed by ultra performance liquid chromatography (UPLC). Results showed that the AFE stored at zero hours had higher total phenol content (47.32 mg gallic acid equivalent (GAE)/g of extract) and free radical scavenging activity (50.38%) at 500 µg/mL significantly compared to other extracts. The total flavonoid content of all extracts showed no difference level except for the extract at 120 hours of storage. The proliferation inhibition test on MCF-7 cells at 100 µg/mL showed that AFE stored at 72, 96 and 144 hours could inhibit MCF-7 cells above 50%. The storage time variation of Andaliman fruit may affect the total phenolic and flavonoid content, and also activity of free radical scavenging and proliferation inhibition on MCF-7. The UPLC analysis founded the major compound of AFE was predicted as α-sanshool. Analysis of chemicals substance in Andaliman fruit with varying of storage time need to be conducted to evaluated alteration of secondary metabolites contained in Andaliman fruit.

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Published

2024-08-26