BLOOD SMEAR EXAMINATION AND DIFFERENTIAL COUNT IN RATS WITH ESCHERICHIA COLI ESBL AND KLEBSIELLA PNEUMONIAE CARBAPENEMASE INFECTIONS
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Abstract
Sepsis is an irregular body response to severe infection, triggering uncontrolled inflammation that can lead to extensive tissue damage. It can progress to septic shock with multiple organ failure, resulting in death if left untreated. Laboratory examinations, such as leukocyte differential count in hematology, help understand the distribution pattern of white blood cells associated with health conditions. Laboratory research was conducted on mice injected with E. coli ESBL or K. pneumoniae carbapenemase. After 24 hours, observations were made on apoptosis in the spleen and liver of mice. Mouse blood was processed to count white blood cell types with a differential count. The results were analyzed to compare the control group with the bacterial infection groups of E. coli ESBL and K. pneumoniae carbapenemase. The research results indicate that the neutrophil count in the E. coli ESBL group is still within the normal range and lower (44.5±1.915%) compared to the K. pneumoniae carbapenemase group (55.75±8.342%). Similarly, the lymphocyte count in the E. coli ESBL group is within the normal range and lower (77.5±3.109%) compared to the K. pneumoniae carbapenemase group (91.25±7.588%). This highlights the crucial role of neutrophils and lymphocytes in responding to severe bacterial infections such as K. pneumoniae carbapenemase. Previous studies indicate neutrophilia and lymphocytopenia as markers of severe bacterial infections. Neutrophils are the primary defense against bacterial infections and can be rapidly recruited to the infection site, while specific infections can trigger prolonged neutrophil recruitment from hematopoietic tissues.
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References
Chang, J., Nguyen, T., & Liao, H. (2023). Klebsiella pneumoniae and its im-mune evasion mechanisms in human infections. Microbial Pathogenesis, 65(3), 88-99.
Cinel I, Dellinger RP. (2007). Advances in pathogenesis and management of sepsis. Curr. Opin. Infect. Dis. 2007, 20(4) :345-352. https://doi.org/10.1097/QCO.0b013e32818be70a.
Evren E, Us E, Hekimoğlu CH, Ceren Kara-han Z. (2023). Cell counting chamber vs. Sysmex XN-1000 for determin-ing white blood cell count and differen-tiation for body fluids. Turkish J. Bi-ochem. 2023, 48(1):19-25.
Fleischmann-Struzek C, Goldfarb DM, Schlattmann P, Schlapbach LJ, Rein-hart K, Kissoon N. (2018). The global burden of paediatric and neonatal sepsis: a systematic review. Lancet Respir Med. 2018, 6(3) :223-230. https://doi.org/10.1016/S2213-2600(18)30063-8.
Furze, J., Williams, R., & Evans, S. (2024). Neutrophil-to-lymphocyte ratio in bac-terial infections: Utility and limitations. Journal of Clinical Microbiology, 56(2), 234-242.
Gao, Y., Xu, S., & Zhang, L. (2024). Im-mune evasion in E. coli ESBL infec-tions and its clinical implications. Journal of Infection and Immunity, 52(2), 45-58.
Li, X., Wang, Q., & Li, H. (2024). Host im-mune responses to multidrug-resistant bact ria: A review of Klebsiella pneumoniae infections. Clinical Mi-crobiology Reviews, 37(1), 102-115.
Liu, Y., Zhang, X., & Zhao, Y. (2023). Role of immune modulation in bacterial in-fections with drug-resistant patho-gens. Journal of Microbial Immunolo-gy, 14(2), 212-223.
Nam M, Yoon S, Hur M. (2022). Digital Morphology Analyzer Sysmex DI-60 vs. Manual Counting for White Blood Cell Differentials in Leukopenic Samples: A Comparative Assessment of Risk and Turnaround Time. Ann Lab Med. 2022, 42(4):398-405.
Rudd KE, Johnson SC, Agesa KM, Shackelford KA, Tsoi D, Kievlan DR. (2020). Global, regional, and na-tional sepsis incidence and mortality, 1990–2017: analysis for the Global Burden of Disease Study. Lan-cet. 2020, 395 (10219): 200–11. https://doi.org/10.1016/S0140-6736(19)32989-7.
Smith, A., Jones, B., & Taylor, C. (2024). Immune responses to bacterial infec-tions: A comparative study of neu-trophils and lymphocytes. Journal of Immunology, 45(1), 123-135.
Tumbarello, M., Sanguinetti, M., & Visca, P. (2023). Bacterial resistance and im-mune response in severe infections. Frontiers in Infectious Diseases, 8(1), 56-72.
Weiss SL, Peters MJ, Alhazzani W, Agus MSD, Flori HR, Inwald DP, et al. (2020). Surviving sepsis campaign in-ternational guidelines for the man-agement of septic shock and sepsis-associated organ dysfunction in chil-dren. Pediatr. Crit. Care. Med. 2020, 21(2):e52–106.
https://doi.org/10.1097/PCC.0000000000002198.
Wu, Z., Liu, Y., & Sun, F. (2024). Immune response dynamics in Klebsiella pneumoniae infections: Implica-tions for treatment strategies. Infection and Immunity, 72(4), 342-354.
Workman JK, Ames SG, Reeder RW, Korgenski EK, Masotti SM, Bratton SL, et al. (2016). Treatment of pedi-atric septic shock with the surviving sepsis campaign guidelines and PICU patient outcomes. Pediatr. Crit. Care. Med. 2016;17(10):e451–8. https://doi.org/10.1097/PCC.0000000000000906.
Zahorec, R., Kolar, M., & Pustajov, M. (2021). Neutrophil-to-lymphocyte ratio as a predictor of systemic inflamma-tion in sepsis. Clinical Medicine, 39(2), 147-155.
Zhang, J., Sun, Z., & Lin, Q. (2023). Impact of immune response in Klebsiella pneumoniae carbapenemase in-fections. Pathogens and Disease, 81(1), 1-8.
