PENGARUH MEDIA DASAR DAN NAA PADA INDUKSI IN VITRO AKAR TUNAS KELAPA SAWIT (Elaeis guineensis)
Article Sidebar
Main Article Content
Abstract
Perbanyakan klonal tanaman kelapa sawit menggunakan teknik kultur jaringan menghasilkan persentase tunas berakar yang rendah. Dalam upaya meningkatkan persentase tunas berakar yang lebih seragam maka dilakukan metode induksi akar yang didukung oleh penggunaan media dasar dan penambahan zat pengatur tumbuh 1-naphthaleneacetic acid (NAA). Penelitian ini bertujuan untuk menganalisis pengaruh kombinasi media dasar dan konsentrasi NAA yang optimum dalam menginduksi akar tunas kelapa sawit secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) faktorial yang terdiri dari 2 faktor. Faktor pertama adalah jenis media dasar yang terdiri dari media Murashige and Skoog (MS) dan MS Modifikasi (MSM). Faktor kedua adalah konsentrasi NAA yang terdiri dari 0; 0,05; 0,1; dan 0,2 ppm. setiap perlakuan diulang sebanyak 10 kali. Hasil penelitian menunjukkan bahwa penggunaan media MSM lebih baik daripada MS, dan konsentrasi NAA yang paling optimum adalah 0,05 dan 0, 1 ppm dalam menginduksi akar kelapa sawit secara in vitro. Selain itu kombinasi perlakuan MSM+NAA 0,1 ppm memiliki hasil yang paling optimum dalam induksi akar kelapa sawit secara in vitro.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
a). Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Attribution-NonCommercial-ShareAlike 4.0 International that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b). Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
References
Arimarsetiowati R, Ardiyani F (2012) Pengaruh penambahan auksin terhadap pertunasan dan perakaran kopi arabika perbanyakan somatik embriogenesis. J Pelita Perkebunan 28:82-90. doi: 10.22302/iccri.jur.pelitaperkebunan.v28i2.201
Arlianti T, Syahid SF, Kristina NN, Rostiana O (2013) Pengaruh auksin IAA, IBA, dan NAA terhadap induksi perakaran tanaman Stevia (Stevia rebaudiana) secara in vitro. Jurnal Buletin Littro. 24:57–62. doi: 10.21082/bullittro.v24n2.2013.%25p
Balzon TA, Luis ZG, Scherwinski-Pereira JE (2013) New approaches to improve the efficiency of somatic embryogenesis in oil palm (Elaeis guineensis Jacq.) from mature zygotic embryos. In Vitro Cell Dev Biol – Plant 49:41–50. doi: 10.1007/s11627-012-9479-3
Basuchaudhuri P (2016) 1-Naphthaleneacetic acid in rice cultivation. Curr Sci 110:52–56. doi: 10.18520/cs/v110/i1/52-56.
Chuanjun X, Zhiwei R, Ling L, Biyu Z, Junmei H, Wen H, Ou H (2015) The effects of polyphenol oxidase and cycloheximide on the early stage of browning in Phalaenopsis explants. Hortic Plant J 1:172–180. doi: 10.16420/j.issn.2095-9885.2015-0030
Costa JM, Heuvelink E, Van de Pol P (2017) Propagation by cuttings. In Reference module in life sciences Elsevier. doi: 10.1016/B978-0-12-809633-8.05091-3
Foster BP, Sitepu B, Setiawati U, Kelanaputra ES, Nur F, Rusfiandi H, Rahmah S, Ciomas J, Anwar Y, Bahri S, Caligari PDS (2017) Oil palm (Elaeis guineensis). In Genetic improvement of tropical crops pp 241–290. Springer Int Pub. doi: 10.1007/978-3-319-59819-2_8
Gamborg OL, Murashige T, Thorpe TA, Vasil K (1976) Plant tissue culture media. In Vitro 12:473–478. doi: 10.1007/BF02796489
George EF, Hall MA, de Klerk G-J (2008) Plant propagation by tissue culture 3rd Edition. Springer, Dordrecht, The Netherlands
Gomes HT, Bartos PMC, Scherwinski-Pereira JE (2015) Optimizing rooting and survival of oil palm (Elaeis guineensis) plantlets derived from somatic embryos. In Vitro Cell Dev Biol – Plant 51:111–117. doi: 10.1007/s11627-015-9669-x
Hazarika BN (2006) Morpho-physiological disorders in in vitro culture of plants. Sci Hortic 108:105–120. doi:10.1016/j.scienta.2006.01.038
Ibrahim MSD, Hartati RS, Rubiyo, Purwito A, Sudarsono (2013). Direct and indirect somatic embryogenesis on arabica coffee (Coffea arabica). Indones J Agricultural Sci 14:79–86. doi:10.21082/ijas.v14n2.2013.p79-86
Nizam K, Te-chato S (2009) Optimizing of root induction in oil palm plantlets for acclimatization by some potent plant growth regulators (PGRs). J Agric Technol 5:371–383
Nwaoguala CNC, Shittu HO (2018) Effects of growth regulators and type-variety of oil palm (Elaeis guineensis Jacq.) on direct organogenesis. Not Sci Biol 10:251–258. doi: 10.25835/nsb10210234
Pierik RLM (1997) In vitro culture of higher plants. Springer Science, Dordrecht
Ridhawati A, Dyah Anggraeni TDA,Purwati RD (2017). Pengaruh komposisi media terhadap induksi tunas dan akar lima. genotipe tanaman Agave pada kultur in vitro. Bul Tanam Tembakau, Serat Miny Ind 9:1–9. doi: 10.21082/btsm.v9n1.2017.1-9
Riyadi I, Sumaryono (2010) Pembentukan akar in vitro planlet kelapa sawit (Elaeis guineensis Jacq.) dalam medium cair dengan penambahan auksin. Menara Perkebunan 78:19–24. doi: 10.22302/iribb.jur.mp.v78i1.76
Saad AIM, Elshahed AM (2012). Plant tissue culture media. In Leva A and Rinaldi LMR (Ed.) Recent Advances in Plant In Vitro Culture pp 29–40. IntechOpen, Winchester
Sarmast MK, Salehi H, Khosh-Khui M (2013) Seismomorphogenesis: a novel approach to acclimatization of tissue culture regenerated plants. 3 Biotech 4:599–604. doi: 10.1007/s13205-013-0191-8
Shintiavira H, Soedarjo M, Suryawati S, Winarto B (2012) Studi pengaruh substitusi hara makro dan mikro media MS dengan pupuk majemuk dalam kultur in vitro krisan. J Hort 22:334–341. doi: 10.21082/jhort.v22n4.2012.p334-341
Shirin F, Parihar NS, Shah SN (2015) Effect of nutrient media and KNO3 on in vitro plant regeneration in Saraca asoca (Roxb.) Willd. Amer J Plant Sci 6:3282–3292. doi: 10.4236/ajps.2015.619320
Thawaro S, Te-chato S (2010). Effect of culture medium and genotype on germination of hybrid oil palm zygotic embryos. Sci Asia 36:26–32. doi: 10.2306/scienceasia1513-1874.2010.36.026
Thuzar M, Vanavichit A, Tragoonrung S, Jantasuriyarat C (2011) Efficient and rapid plant regeneration of oil palm zygotic embryos cv. ‘Tenera’ through somatic embryogenesis. Acta Physiol Plant 33:123–128. doi: 10.1007/s11738-010-0526-6
Tolera B (2016) Effects of Naphthalene Acetic Acid (NAA) and Indole-3-Butyric Acid (IBA) on in vitro rooting of sugarcane (Saccharum officinarum L.) micro-shoots. J Biotecnol Biomater 6:215. doi: 10.4172/2155-952X.1000215
Yan YH, Li JL, Zhang XQ, Yang WY, Wan Y, Ma YM, Zhu YQ, Peng Y, Huang LK (2014) Effect of naphthalene acetic acid on adventitious root development and associated physiological changes in stem cutting of Hemarthria compressa. Plos One 9: e90700. doi: 10.1371/journal.pone.0090700
Yunianti (2017) Pengaruh pemberian auksin pada induksi akar secara in vitro dan mikoriza secara ex vitro pada akar bibit tanaman kelapa sawit (Elaeis guineensis Jacq.). Skripsi, Universitas Teknologi Sumbawa
Yunita R, Mariska I, Purnamaningsih R, Lestari EG, Utami S (2016) Induksi akar tunas kelapa sawit (Elaeis guinensis Jacq.) secara in vitro dan ex vitro. J Littri 22:37–42. doi: 10.21082/littri.v22n1.2016.37-42
Yusnita, Hapsoro D (2011) In vitro callus induction and embryogenesis of oil palm (Elaeis guineensis Jacq.) from leaf explants. Hayati J Biosci 18:61–65. doi: 10.4308/hjb.18.2.61
Zhang K, Wu Y, Hang H (2019) Differential contributions of NO3− /NH4+ to nitrogen use in response to a variable inorganic nitrogen supply in plantlets of two Brassicaceae species in vitro. Plant Methods 15:86. doi: 10.1186/s13007-019-0473-1