EVALUATION OF CREAM FORMULATION FROM NANOEMULSION Euphorbia tirucalli L. WITH STEARIC ACID VARIATIONS
Main Article Content
Abstract
The macerate of Euphorbia tirucalli L. contains alkaloids, flavonoids, saponins, steroids, terpenoids, and tannins with antibacterial potential for acne management. This study formulated a nanoemulsion of E. tirucalli extract via the high-energy method, which was incorporated into cream formulations with varying stearic acid concentrations (F1;7.5%, F2;9%, F3;12%, F4;14.5%) and physical quality evaluation was carried out. The physical quality test of the cream preparations found that concentrations of stearic acid significantly affected the organoleptic properties (color and skin feels after application), spreadability 4.86-6.55 cm, water washability 8.5-19.1 mL, and viscosity 15557-31433 cPs. Meanwhile, homogeneity, phase separation, and pH of the preparation were not found the significantly different. An increase in stearic acid was directly proportional to washability and viscosity, but inversely proportional to spreadability. The formulations met the physical criteria for an acceptable cream preparation, except for pH, which was excessively alkaline. F2 and F3 establishing them as the most suitable formulations for further development. The antibacterial test against Propionibacterium acnes using the well diffusion agar assay, resulted; positive control (Mediklin; clindamycin 0.01%) in strong activity, 5% extract was in the weak activity, 10% and 20% extract was in moderate activity, 30% extract showed strong activity which is comparable to the positive control, and 20% nanoemulsion had no activity. The Spearman’s Rho test confirmed a strong correlation between extract concentration and antibacterial potency (r = 0.927).
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References
Abd Wahab NZ, Malza NMNM, Rukayadi Y (2024) Phytochemical screening and evaluation of antibacterial, antican-didal, and sporicidal properties of Eu-phorbia tirucalli extract in Terengganu, Malaysia. HAYATI J Biosci 31:903–915. doi: 10.4308/hjb.31.5.903-915
Adawiyah R, Mukti AW, Sanulingga AS (2024) Perbandingan aktivitas ekstrak ranting patah tulan (Euporbia tirucalli) dan daun yodium (Jatropha muitifida) terhadap Staphylococcus Aureus. J Kesehat Tambusai 5:10071–10077
Afifah N, Riyanta AB, Amananti W (2023) Pengaruh waktu maserasi terhadap hasil skrining fitokimia pada ekstrak daun mangga harum manis (Man-gifera Indica L.). J Cryst Publ Penelit Kim Ter 5:54–61. doi: 10.36526/jc.v5i1.2634
Arifin SHAG, Firdhausi NF, Hidayati I, Fai-zah H (2025) Phytochemical screen-ing and antimicrobial activity of com-bination of Piper betle and Moringa oleifera extracts. Indones J Pharm Sci Technol 12:184–191. doi: 10.24198/ijpst.v12i2.45288
Armillei MK, Lomakin IB, Del Rosso JQ, Grada A, Bunick CG (2024) Scientific rationale and clinical basis for clindamycin use in the treatment of dermatologic disease. Antibiotics 13:270. doi: 10.3390/antibiotics13030270
Febriyanto F, Hanifa NI, Muliasari H (2021) Penetapan kadar fenolik total ekstrak kulit buah kopi robusta (Coffea canephora L.) di pulau Lombok. Lum-bung Farm J Ilmu Kefarmasian 2:89–95
Fox L, Csongradi C, Aucamp M, Du Plessis J, Gerber M (2016) Treatment modali-ties for acne. Molecules 21:1063. doi: 10.3390/molecules21081063
Geetanjali R, Sreejit V, Sandip P, Preetha R (2021) Preparation of Aloe vera muci-lage- ethyl vanillin nano-emulsion and its characterization. Mater Today Proc 43:3766–3773. doi: 10.1016/j.matpr.2020.10.990
Husni P, Pratiwi AN, Baitariza A (2019) Formulasi krim ekstrak etanol daun kelor (Moringa oleifera Lamk). J Ilm Farm Farmasyifa 2:101–110. doi: 10.29313/jiff.v2i2.4796
Iskandar D, Putri DAM, Hidayani R (2024) Uji kualitatif senyawa metabolit sekunder ekstrak daun malapari (Pongamia pinnata L. Pierre) pada pelarut etanol dan n-heksana sebagai kandidat sunscreen. BADAA J Ilm Pendidik Dasar 6:107–114. doi: 10.37216/badaa.v6i1.1400
Kinho J, Arini DID, Tabba S, Kama H, Kafiar Y, Shabri S, Karundeng MC (2011) Tumbuhan obat tradisional di Sulawasi Utara Jilid I. Balai Penelitian Kehutanan Manado, Manado
Krutmann J, Moyal D, Liu W, Kandahari S, Lee G-S, Nopadon N, Xiang LF, Seité S (2017) Pollution and acne: is there a link? Clin Cosmet Investig Dermatol 10:199–204. doi: 10.2147/CCID.S131323
Kumalasari E, Mardiah A, Sari AK (2020) Formulasi sediaan krim ekstrak daun bawang Dayak (Eleutherine palmifolia (L) Merr) dengan basis krim tipe A/M dan Basis Krim tipe M/A. J Farm In-dones AFAMEDIS 1:23–33. doi: 10.61609/afamedis.v1i1.2
Laoli NS (2018) Uji aktivitas antibakteri ekstrak etanol daun bandotan (Agera-tum conyzoides L.) terhadap bakteri Bacillus substilis dan Proteus vulgar-is. Bachelor’s thesis, Universitas Su-matera Utara
Lestario JR, Sari IP, Andareza A, Fadillah S, Rachmaniar R (2024) Karakteristik nanoemulsi isolat brazilin dari tana-man kayu secang (Caesalpinia sap-pan L.) asli Indonesia. Maj Farmaseti-ka 9:205–215
Masniawati A, Johannes E, Winarti W (2021) Analisis fitokimia umbi talas Jepang Colocasia esculentai L.(Schott) var. antiquorum dan talas kimpul Xanthosoma sagittifolium L.(Schott) dari dataran rendah. J Ilmu Alam Lingkung 12:7–14
McFarland J (1907) The nephelometer:an Instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. J Am Med Assoc XLIX:1176–1178. doi: 10.1001/jama.1907.25320140022001f
Nola F, Putri GK, Malik LH, Andriani N (2021) Isolasi senyawa metabolit sekunder steroid dan terpenoid dari 5 tanaman. Syntax Idea 3:1612–1619
Nurhalijah SD, Nina C, Romadhona A, Mau-lani N, Rahayu MS (2024) Analisis ko-relasi Spearman untuk mengetahui hubungan antara penggunaan media sosial dan tingkat produktivitas akad-emis mahasiswa agribisnis (Studi ka-sus : Universitas Sultan Ageng Tirtayasa). J Ilm Wahana Pendidik 10:800–809. doi: 10.5281/zenodo.13766003
Qomaliyah EN, Indriani N, Rohma A, Islam-iyati R (2023) Skrining fitokimia, kadar total flavonoid dan antioksidan daun cocor bebek. Curr Biochem 10:1–10
Retnowati A, Susan D (2019) Kekayaan jenis jamur. In: Retnowati A, Ruga-yah, Rahajoe JS, Arifiani D (eds) Sta-tus keanekaragaman hayati Indone-sia: Kekayaan jenis tumbuhan dan jamur Indonesia. LIPI, Jakarta
Rini AA, Supriatno S, Rahmatan H (2017) Phytochemical screening and antibac-terial test of ethanolic extract of kawista (Limonia acidissima L.) from Aceh Besar against Escherichia coli. J Ilm Mhs Pendidik Biol 2
Rowe RC, Sheskey PJ, Weller PJ (2009) Handbook of pharmaceutical excipi-ents. Pharmaceutical Press, London
Sadiah HH, Cahyadi AI, Windria S (2022) Kajian daun sirih hijau (Piper betle L) sebagai antibakteri. J Sain Vet 40:128–138. doi: 10.22146/jsv.58745
Saepudin S, Hartono K, Wasih EA (2024) Formulasi krim anti-jerawat ekstrak daun kesum (Polygonum minus Huds) dengan variasi konsentrasi asam stearat. Farmasains J Ilm Ilmu Kefarmasian 11:66–75. doi: 10.22236/farmasains.v11i2.14707
Saryanti D, Setiawan I, Safitri RA (2019) Optimasi asam stearat dan TEA pada formula sediaan krim ekstrak kulit pi-sang kepok (Musa paradisiaca L.). J Ris Kefarmasian Indones 1:225–237
Septiyana E, Dewi ERS, Sumarno (2022) Uji daya hambat ekstrak kulit buah ketapang muda (Terminalia catappa L.) terhadap pertumbuhan bakteri Propionibacterium acnes. SNSE VIII 1:80–90
Setiorini MS, Soegihardjo C. J, Atmodjo K (2014) Potensi antimikrobia krim ekstrak ranting patah tulang (Euphor-bia tirucalli Linn.) terhadap Propioni-bacterium acnes ATCC 11827 dan Candida albicans ATCC 24433. J Farm Sains Komunitas 11:64–71
Singh D, Kumar A (2015) Effects of nano silver oxide and silver ions on growth of Vigna radiata. Bull Environ Contam Toxicol 95:379–384. doi: 10.1007/s00128-015-1595-4
Sukma IWA, Harsojuwono BA, Arnata IW (2017) Pengaruh suhu dan lama pemanasan ekstraksi terhadap rendemen dan mutu alginat dari rumput laut hijau Sargassum sp. J Rekayasa Manaj Agroindustri 5:71–80
Swastini DA, Yanti NLGT, Udayana NK, Arisanti CIS, Wirasuta IMAG (2015) Uji sifat fisik cold cream kombinasi ekstrak kulit buah manggis (Garcinia mangostana L.), daun binahong (Anredera cordifolia), herba pegagan (Centella asiatica) sebagai antiluka bakar. J Farm Udayana 4:279721
Thomas NA, Suryadi AMA, Latif MS, Hutu-ba AH, Susanti S (2024) Formulasi dan uji stabilitas fisik krim pelembab ekstrak rumput laut (Eucheuma cot-tonii). Indones J Pharm Educ 4:1–9. doi: 10.37311/ijpe.v4i1.20522
Vasam M, Korutla S, Bohara RA (2023) Ac-ne vulgaris: A review of the patho-physiology, treatment, and recent nanotechnology based advances. Bi-ochem Biophys Rep 36:101578. doi: 10.1016/j.bbrep.2023.101578
Widyastuti AI, Saryanti D (2023) Formulasi dan evaluasi sediaan nanoemulsi ekstrak umbi bawang putih (Allium sa-tivum L.): Formulation and evaluation of nanoemulsion of garlic (Allium sa-tivum L.) extract. J Sains Kesehat 5:178–185
Xu W, Gu H, Zhu X, Zhong Y, Jiang L, Xu M, Song A, Hao J (2015) CO2-controllable foaming and emulsifica-tion properties of the stearic acid soap systems. Langmuir 31:5758–5766. doi: 10.1021/acs.langmuir.5b01295
Yuliantari NWA, Widarta IWR, Permana IDGM (2017) Pengaruh suhu dan waktu ekstraksi terhadap kandungan flavonoid dan aktivitas antioksidan daun sirsak (Annona muricata L.) menggunakan ultrasonik. Media Ilm Teknol Pangan Sci J Food Technol 4:35–42
Zulfa E, Novianto D, Setiawan D (2019) Formulasi nanoemulsi natrium diklofenak dengan variasi kombinasi Tween 80 dan Span 80: Kajian karak-teristik fisik sediaan. Media Farm In-dones 14:1471–1477
