EFFECT OF SALINITY AND GROWTH MEDIUM ON Symbiodinium sp. ISOLATED FROM GIANT CLAM

Authors

  • Maria Goretti Lily Panggabean Marine Resources Division. Research Centre for Oceanography
  • Rory A. Hutagalung Faculty of Biotechnology. Atma Jaya Catholic University
  • Eveline Ayu Faculty of Biotechnology. Atma Jaya Catholic University

DOI:

https://doi.org/10.14203/mri.v34i2.513

Keywords:

Symbiodinium sp., Hippopus hippopus, growth medium, f/2, modified GPM

Abstract

An experiment on isolate Symbiodinium sp. from Hippopus hippopus was carried out to determine growth medium and optimum salinity for in vitro culture of the symbiont. The split-plot factorial design with two factors was applied with 3 replications. The first factor was medium with 2 levels, namely f/2 and modified GPM. The second factor was salinity with five levels i.e. 20, 25, 30, 35 and 40 PSU. All treatments were incubated under continuous light (2600 lux) and 25°C. The maximum growth was reached at day 16 and there was significant interaction (p<0.05) between medium and salinity. The maximum density (64.8 x 104cell/ml) was found in media f/2 and salinity 35 PSU.

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Published

2026-01-08

Issue

Vol. 34 No. 2 (2009)

Section

Articles