THERMOHALOPHILIC BACTERIA PRODUCING THERMOSTABLE LIPASE FROM WAWOLESEA HOT SPRING, SOUTHEAST SULAWESI
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Abstract
This study aimed to isolate and characterize thermohalophilic bacteria producing lipase and to purify thermostable lipase from the Wawolesea hot spring in Southeast Sulawesi. A total of 24 isolates were obtained, all of which demonstrated lipase-producing capability. Three selected isolates (BT2.M3, CT2.M4, and DT1.M2) were identified as Gram-negative bacilli and showed lipase activities ranging from 0.17 to 21 U/mL, with the highest activity recorded for BT2.M3 at 50°C and pH 6. Purification through ammonium sulfate fractionation yielded the highest activity in the 60–80% saturation fraction (43 U/mL) at pH 6–7 and 50–60°C. Protein analysis showed the highest concentration in the 80% dialysis fraction (0.954 mg/mL). These findings highlight the potential of Wawolesea thermohalophilic bacteria as a valuable source of thermostable lipase for biotechnological applications.
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References
Bhardwaj, K., Kumar, A., & Sharma, R. (2020). Structural stability of thermo-stable lipases under high-temperature conditions. Enzyme Research, 2020, Article ID 8851237, 1–12. https://doi.org/10.1155/2020/8851237
Cavicchioli, R. (2016). Microbial ecology of Antarctic aquatic systems. Nature Re-views Microbiology, 14, 691–706. https://doi.org/10.1038/nrmicro.2016.107
Chatelet, D., Martin, D., & Bruneau, A. (2022). Salt precipitation behavior of thermostable lipases. Biotechnology Letters, 44, 455–463.
Contesini, F. J., Melo, R. R., & Sato, H. (2020). Structure–function relation-ships in microbial lipases. Food Bio-technology, 34(3), 192–214.
Feller, G., Gerday, C., & Aittaleb, M. (2012). Adaptation of proteins to cold and heat: Extremophilic enzymes. Bio-chimie, 94, 235–245.
Fonseca, T. S., Lima, V. M., & Pereira, E. M. (2021). Olive oil as an inducer of microbial lipase production. Biocataly-sis and Agricultural Biotechnology, 36, 102–109.
Fox, P. F., Guinee, T. P., Cogan, T. M., & McSweeney, P. L. H. (2004). Funda-mentals of cheese science. Springer.
Gaur, N., Narasimhulu, K., & Chandra, T. (2016). Temperature optima of micro-bial lipases: A review. Applied Micro-biology and Biotechnology, 100(3), 1257–1270. https://doi.org/10.1007/s00253-015-7231-9
Gupta, R., Gupta, N., & Rathi, P. (2004). Bacterial lipases: An overview of pro-duction, purification and biochemical properties. Applied Microbiology and Biotechnology, 64, 763–781. https://doi.org/10.1007/s00253-004-1568-8
Gurumurthy, D. M., et al. (2020). Thermo-philic bacteria and thermostable en-zymes in industrial biotechnology: Challenges and opportunities. Bio-technology Advances, 43, 107581. https://doi.org/10.1016/j.biotechadv.2020.107581
Jaeger, K. E., & Eggert, T. (2002). Lipases for biotechnology. Current Opinion in Biotechnology, 13, 390–397. https://doi.org/10.1016/S0958-1669(02)00341-5
Jamaluddin, M., et al. (2018). Isolation and screening of thermophilic lipase-producing bacteria from Indonesian geothermal areas. Indonesian Journal of Biotechnology, 23(1), 45–52. https://doi.org/10.22146/ijbiotech.34100
Kouker, G., & Jaeger, K. E. (1987). Specific and sensitive plate assay for bacterial lipases. Applied and Environmental Microbiology, 53(1), 211–213.
Madigan, M. T., Bender, K. S., Buckley, D. H., Sattley, W. M., & Stahl, D. A. (2018). Brock biology of microorgan-isms (15th ed.). Pearson.
Muzuni, M., Jamaluddin, J., Suriana, S., Ar-diansyah, A., & Yanti, N. A. (2024). Skrining bakteri termohalofilik penghasil L-asparaginase dari sumber air panas Wawolesea Sulawesi Tenggara dan uji aktivitas enzimnya. Alchemy: Jurnal Penelitian Kimia, 20(1), 12–20.
Neale, G. H., Lampen, J. O., & Wilson, R. (1987). Protein precipitation and sta-bility in ammonium sulfate solutions. Methods in Enzymology, 148, 22–33. https://doi.org/10.1016/0076-6879(87)48039-6
Oren, A. (2015). Halophilic microbial com-munities and their environments. Cur-rent Opinion in Biotechnology, 33, 119–124. https://doi.org/10.1016/j.copbio.2014.12.005
Purkan, P., Nurfadila, N., & Dewi, S. R. (2020). Growth kinetics and lipase production in thermophilic bacteria. Journal of Applied Microbiology, 129(3), 533–542. https://doi.org/10.1111/jam.14626
Radji, M. (2010). Bioteknologi farmasi: Mikrobiologi dan aplikasinya. Pustaka Obor Indonesia.
Salwoom, L., Rahman, R. A., & Salleh, A. B. (2019). Gram-negative bacteria as sources of industrial lipases. Journal of Molecular Catalysis B: Enzymatic, 164, 68–78. https://doi.org/10.1016/j.molcatb.2019.111297
Schimel, J. (2007). Soil microbial communi-ty structure in hot-spring ecosystems. Environmental Microbiology, 9, 2941–2951. https://doi.org/10.1111/j.1462-2920.2007.01371.x
Sharma, R., Chisti, Y., & Banerjee, U. C. (2001). Production, purification,
characterization, and applications of lipases. Biotechnology Advances, 19, 627–662. https://doi.org/10.1016/S0734-9750(01)00086-6
Sharma, S., Sehgal, R., & Gupta, R. (2017). Stability and activity of bacterial lipas-es under environmental stress. Bio-chimica et Biophysica Acta (BBA) – Proteins and Proteomics, 1865, 1–12. https://doi.org/10.1016/j.bbapap.2016.09.015
Sholeha, F., & Agustini, T. W. (2021). Thermohalophilic bacteria from Indo-nesian geothermal sites. Jurnal Biolo-gi Tropis, 21(3), 522–530.
Stetter, K. O. (2013). A brief history of ex-tremophiles. FEMS Microbiology Let-ters, 364, 1–10. https://doi.org/10.1111/1574-6968.12100
Ventosa, A., Fernández, A. B., León, M. J., Sánchez-Porro, C., & Rodriguez-Valera, F. (2015). Halophilic bacteria and archaea in hypersaline environ-ments. Advances in Microbial Physi-ology, 66, 29–74. https://doi.org/10.1016/bs.ampbs.2015.03.001
White, D. E., & Brannock, W. W. (1950). Sil-ica and cation content of thermal wa-ters as indicators of geological pro-cesses. American Journal of Science, 248(10), 737–753. https://doi.org/10.2475/ajs.248.10.737
