Enterococcus faecium 1.15 Isolated from Bakasam Showed Milk Clotting Activity

Authors

  • Wendry Setiyadi Putranto Graduate School, Bogor Agricultural University (ITB)
  • Kusmajadi Suradi Padjadjaran University
  • Hartati Chairunnisa Padjadjaran University
  • Apon Zaenal Mustopa Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI)
  • Harsi Dewantari Kusumaningrum Bogor Agricultural University
  • Maggy Thenawidjaja Suhartono Bogor Agricultural University

Keywords:

lactic acid bacteria, milk clotting activity, rennin-like protease

Abstract

Rennin-like protease of many microorganisms behave similarly to chymosin and are potential alternatives of rennet. The lactic acid bacteria with milk clotting activity were isolated from Bakasam, an Indonesian traditional fermented meat. Screening assay was carried out using modified method of skim milk agar and milk clotting activity test, and the isolate was then identified using 16S rRNA. We found 4 isolates that showed MCA of 18-20 SU/mL. Identification using 16S rRNA indicated that the isolate ALG.1.15 was 99% identical with Enterococcus faecium. The isolate potentially produced renin-like protease to subtitute renin from veal.

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Published

2025-09-11

How to Cite

Putranto, W. S., Suradi, K., Chairunnisa, H., Mustopa, A. Z., Kusumaningrum, H. D., & Suhartono, M. T. (2025). Enterococcus faecium 1.15 Isolated from Bakasam Showed Milk Clotting Activity. Annales Bogorienses, 21(1), 9–14. Retrieved from https://ejournal.brin.go.id/annales/article/view/7710

Issue

Vol. 21 No. 1 (2017): Annales Bogorienses

Section

Articles