INDUCING THE IN VITRO ROOTING OF SAGO PALM (Metroxylon sagu Rottb.) USING AUXIN
Article Sidebar
Main Article Content
Abstract
ago palm (Metroxylon sagu Rottb) has huge potential as food, energy and industrial bioresources. In vitro culture of sago palm was performed in Biotech Center, BPPT in order to obtain a large-scale of mass clonal propagation of superior genotypes. Nevertheless, the main obstacle for the sago palm in vitro propagation was rooting formation. The purpose of our study was to obtain the best hormones combination for root induction on sago palm shoots in vitro. The young suckers (15-20 cm) obtained from Rangkasbitung area, Banten Province, were used as explants. In our study, in vitro rooting was induced by different types and concentrations of auxin, medium strength and solidifying agents. The shoots were cultured on Gamborg media. The result showed that the best level of both hormones IBA and NAA for root induction was 35 ppm. Moreover the solidifying agent of Gelrite gave positive response by stimulating root at the basal-end.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
a). Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Attribution-NonCommercial-ShareAlike 4.0 International that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b). Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
References
Direktorat Jenderal Perkebunan (2013) Peningkatan produksi, produktivitas dan mutu tanaman tahunan: Pedoman teknis pengembangan tanaman sagu. Pedoman teknis pengembangan tanaman sagu tahun 2014. Direktorat Jenderal Perkebunan, Kementerian Pertanian, 24p
Bintoro MH (2003) Potensi pemanfaatan sagu untuk industri dan pangan. Prosiding Seminar Nasional Sagu, Manado 06 Oktober 2003. Pusat Penelitian dan Pengembangan Perkebunan. Bogor
Gamborg OL, Murashige T, Thorpe TA, Vasil IK (1976) Plant tissue culture media. In Vitro 12:473-478
Hisajima S, Jong FS, Arai Y, Sim ES (1991) Propagation and breeding of sago palm (Metroxylon sagu Rottb) plant in vitro: 1. Embryo culture and induction of multiple shoots from sago embryos in vitro. Jap J Trop Agric 35:259-267
Johnson DV (2010) Tropical palms. 2010 revision. Food and Agricultural Organization of United Nation. 242p
Jong FS (1995) Research for the development of sago palm (Metroxylon sagu Rottb) cultivation in Sarawak, Malaysia. PhD Thesis, Wageningen Univ. 139p
Kanro MZ, Rouw A, Widjono A, Syamsuddin, Amisnaipa, Ateka (2003) Tanaman sagu dan pemanfaatannya di Propinsi Papua. J Litbang Pertanian 22:116-124
Karim AA, Tie AP, Manan DMA, Zaidul ISM (2008) Starch from sago (Metroxylon sagu) palm tree – Properties, prospect and challenges as a new industrial source for food and other uses. Comprehensive Reviews in Food Sci & Food Safety 7:215-228
Limbongan J, Hanafiah A, Nggobe M (2005) Pengembangan sagu Papua. Balai Pengkajian Teknologi Pertanian Papua. Badan Penelitian dan Pengembangan Pertanian. Departemen Pertanian
Mashud N (1991) Faktor-Faktor yang harus diperkirakan untuk membudidayakan tanaman sagu. Balai Penelitian Kelapa. Badan Penelitian dan Pengembangan Pertanian. Departemen Pertanian. Manado. Buletin Balitka No 13: 111-116
Riyadi I, Tahardi JS, Sumaryono (2005) The development of somatic embryos of sago palm (Metroxylon sagu Rottb) on solid media. Menara Perkebunan 73:33-40
Sumaryono, Muslihatin W, Ratnadewi D (2012) Effect of carbohydrate source on growth and performance of in vitro sago palm (Metroxylon sagu Rottb) plantlets. Hayati J Biosci 19:88-92
Tahardi JS, Sianipar NF, Riyadi I (2002) Somatic embryogenesis in sago palm (Metroxylon sagu Rottb). In: K Kaimuna, M Okazaki, Y Toyoda, JE Cecil (eds.), New Frontiers of Sago Palm Studies, p. 75-81. Universal Academic Press, Inc. Tokyo, Japan
Tajuddin T, Karyanti, Sukarnih T, Haska N (2014) A revised method for sucker sterilization to support the in vitro propagation of sago palm (Metroxylon sagu Rottb). J Bioteknol Bios Indon 1:21-26
Tajuddin T, Karyanti, Sukarnih T, Haska N (2015) The combination of growth hormones increased in vitro shoots multiplication on sago palm (Metroxylon sagu Rottb). J Bioteknol Bios Indon 2:73-79
Wahid AS (1987) Pengaruh besar anakan, naungan dan penyimpanan terhadap pertumbuhan bibit tanaman sagu (Metroxylon sagu Rottb). Tesis. Fakultas Pertanian, IPB, Bogor